Year 2019, Volume 22 , Issue , Pages 388 - 394 2019-12-31

Gölevez [(Colocasia esculenta (L.)] Yumrularının Etanol Ekstresinin Antioksidan Aktivitesinin Belirlenmesi
Determination of Antioxidant Activity of Ethanol Extract of Gölevez [(Colocasia esculenta (L.)] Tubers

Mehmet AKYÜZ [1]


Bu çalışmada Gölevez (Colocasia esculenta) yumrularının etanol ekstresinin antioksidan aktivitesi DPPH ve ABTS radikallerini giderme aktiviteleri, metal şelatlama aktivitesi ve indirgeme gücü gibi dört farklı antioksidan metotla belirlendi. C.esculenta yumrularının ekstreleri ve standartların en yüksek konsantrasyonda (600 µg mL-1) DPPH radikali üzerindeki giderme etkisi Vitamin C ˃Trolox˃C.esculenta˃BHA sırasına göre azaldı ve sırasıyla % 95.4, 93.6, 83.8 ve 78.8 olarak bulundu. C. esculenta yumrularının ekstreleri ve standartların en yüksek konsantrasyonda (100 µg mL-1) ABTS radikali üzerindeki giderme etkisi Trolox=BHA˃C.esculenta şeklinde azaldı ve sırasıyla % 100, 100 ve 94.6 olarak bulundu. C.esculenta yumrularının ekstreleri ve standartların en düşük konsantrasyonda (100 µg mL-1) metal şelatlama aktiviteleri C. esculenta˃BHA˃Trolox sırasına göre azaldı ve sırasıyla  % 78.0, 76.0 ve 63.5 olarak bulundu. C. esculenta yumrularının ekstreleri ve standartların en yüksek konsantrasyonda (600 µg mL-1) indirgeme güçleri BHA˃Trolox˃C.esculenta şeklinde sıralandı. C.esculenta yumru ekstrelerinin toplam fenolik bileşik ve flavonoid miktarları sırasıyla 2400 mg GAE/kg ekstre ve 2050 mgQE/kg ekstre olarak belirlendi.

In this study, the antioxidant activity of the ethanolic extract of Colocasia esculenta tubers were determined by four different antioxidant tests including DPPH and ABTS radicals scavenging activities, metal chelating activity and reducing power. The scavenging effect of extract of C. esculenta tubers and standards on DPPH radical at the highest concentration (600 µg mL-1) decreased in the following order: Vitamin C˃Trolox˃C. esculenta˃BHA and were found as 95.4, 93.6, 83.8 and 78.8 %, respectively. The scavenging effect of C. esculenta tuber extract and standards on ABTS radical at the highest concentration (100 µg mL-1) decreased in the order: Trolox = BHA˃C. esculenta and were found as 100, 100, 94.6%, respectively. The metal chelating capacity of extract of C. esculenta tubers and standards decreased in the order of C. esculenta˃BHA˃Trolox at lowest concentration (100 µg mL-1) and was found to be 78.0, 76.0, 63.5 %, respectively. Reducing power of extract of C. esculenta tubers and standards at the highest concentration (600 µg mL-1) followed the order: BHA˃Trolox˃C. esculenta. Total phenolic compound and flavonoid amounts of C. esculenta tubers were designated as 2400 mg GAE/kg extract and 2050 mg QE/kg extract, respectively.

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Primary Language en
Subjects Basic Sciences
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-7971-8389
Author: Mehmet AKYÜZ (Primary Author)
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey


Dates

Application Date : July 9, 2019
Acceptance Date : August 23, 2019
Publication Date : December 31, 2019

APA AKYÜZ, M . (2019). Determination of Antioxidant Activity of Ethanol Extract of Gölevez [(Colocasia esculenta (L.)] Tubers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 () , 388-394 . DOI: 10.18016/ksutarimdoga.vi.589216