Cytotoxic and Genotoxic Activity of Turkish Pale Liverwort (Chiloscyphus polyanthos (L.) Corda) Against Human Periferal Blood Lymphocytes
Abstract
Pale Liverwort-Chiloscyphus polyanthos is one of the most common aquatic liverworts that growing in or near the watering streams and lakes. It typically formed extensive matting appearance in the water or on the boulders in the water. In this study, Chiloscyphus polyanthos collected from the Erciyes Mountain were extracted using ethyl acetate, methanol, and water-methanol solvents. Cytotoxic and genotoxic effects of extracts against human peripheral blood lymphocytes were investigated. The genotoxic activity was determined by using the Chromosome Aberration Test. The cytotoxic activities of the extracts were determined by the Mitotic index (MI). The results showed that the extracts had cytotoxic effect and not genotoxic effect. According to this result, we estimate that Chiloscyphus polyanthos can be used as medicinal plant.
Supporting Institution
Niğde Ömer Halisdemir Üniversitesi
Project Number
YULTEP/FEB2014/28
Thanks
This work is supported by the Nigde Omer Halisdemir University (Project No: YULTEP, FEB2014/28).
References
- Alam A. 2012. Some Indian Bryophytes known for their biologically active compounds. International Journal of Applied Biology and Pharmaceutical Technology. 3, 239-246.
- Asakawa Y. Ludwiczuk A. Hashimoto T. 2013a. Cytotoxic and antiviral compounds from bryophytes and inedible fungi. Journal of Pre-clinical and Clinical Research. 7:2, 73-85.
- Asakawa Y. Ludwiczuk A. Nagashima F. 2013b. Phytochemical and biological studies of bryophytes. Phytochemistry. 91, 52-80.
- Atherton I. Bosanquet S. D. Lawley M. 2010. Mosses and Liverworts of Britain And Ireland - A Field Guide. British Bryological Society. Britain.
- Burgess E. Larsen J.L.N. Perry B. 2000. A Cytotoxic sesquiterpene caffeate from the liverwort Bazzania novae-zelandiae. Journal of Natural Products. 63, 537–539.
- Chandra S. D. Chandra A. Barh A. Pankaj R. Pandey K. Sharma I. P. 2017. Bryophytes: Hoard of remedies, an ethno-medicinal review. Journal of Traditional and Complementary Medicine. 7:1, 94–98.
- Çolak E. Kara R. Ezer T. Çelik G.Y. Elibol B. 2011. Investigation of antimicrobial activity of some Turkish pleurocarpic mosses. African Journal of Biotechnology. 10, 12905–12908.
- Dey A. De J.N. 2012. Antioxidative potential of bryophytes: stress tolerance and commercial perspectives: a review. Pharmacologia. 3, 151–159.
- Dey A. Mukherjee A. 2015. Therapeutic potential of bryophytes and derived compounds against cancer. Journal of Acute Disease. 4:3, 236–248.
- Komala I.T. Ito F. Nagashima Yagi Y. Asakawa Y. 2010. Cytotoxic, radical scavenging and antimicrobial activities of sesquiterpenoids from the Tahitian liverwort Mastigophora diclados (Brid.) Nees (Mastigophoraceae). Jornal of Natural Medicines. 64, 417–422.
- Perry N. B. Foster L.M. Lorimer S.D. May B.C. Weavers R.T. 1996. Isoprenyl phenyl ethers from liverworts of the genus Trichocolea: cytotoxic activity, structural corrections and synthesis. Jornal of Natural Medicines. 59, 729-733.
- Rencüzogulları E. İla H.B. Kayraldiz A. Diler S. B. Yavuz A. Arslan M. Kaya F.F. Topaktas M. 2006. The Mutagenic and Antimutagenic Effects of Ecballium elaterium Fruit Juice in Human Peripheral Lymphocytes. Russian Journal of Genetics. 42:6, 623–627.
- Saxena D. Harinder K. 2004. Uses of Bryophytes. Resonance. 9:6, 56-65.
- Suhalka D. 2016. Eco-Friendly Management of Maize Pathogen Thruogh Bryophyte Extract. Journal of Global Pharma Technology. 8:1, 1-4.
- Toyota M. Saito T. Asakawa Y. 1999. The Absolute Configuration of Eudesmane-type Sesquiterpenoids Found in the Japanese Liverwort Chiloscyphus polyanthus. Phytochemistry. 51, 913-920.
- Xie C.F. Sun B. Guo D.X. Gao J. Qu J.B. Lou H. X. 2011. Terpenoids from the Chinese Liverwort Chiloscyphus polyanthus. Helvetica Chimica Acta. 94: 3, 534-538.
- Yağlıoğlu Ş.E. Abay G. Demirtas İ. Yağlıoğlu Ş.A. 2017. Phytochemical screening, antiproliferative and cytotoxic activities of the mosses Rhytidiadelphus triquetrus (Hedw.) Warnst. and Tortella tortuosa (Hedw.) Limpr. Anatolian Bryology 3:1, 31-42.
Türk Soluk Ciğerotu (Chiloscyphus polyanthos (L.) Corda)’nun İnsan Periferal Kan Lenfositlerine Karşı Sitotoksik ve Genotoksik Aktivitesi
Abstract
Chiloscyphus polyanthos, göllerde ve
akarsularda su içinde veya su kenarında yetişen en yaygın akuatik ciğerotlarından
biridir. Tipik olarak, su içerisinde veya su içindeki kayaların üzerinde geniş
paspas görünümü oluşturur. Bu çalışmada, Erciyes Dağı'ndan toplanan Chiloscyphus polyanthos, etil asetat,
metanol ve su-metanol çözücüleri kullanılarak ekstre edildi. Ekstraktların
insan periferal kan lenfositlerine karşı sitotoksik ve genotoksik etkileri
araştırıldı. Genotoksik aktivite, Kromozom Aberrasyon Testi kullanılarak
belirlendi. Ekstraktların sitotoksik aktiviteleri Mitotik indeks (MI) ile
belirlendi. Sonuçlar ekstraktların sitotoksik etkiye sahip olduğunu, genotoksik
etkiye sahip olmadığını gösterdi. Bu sonuca göre Chiloscyphus polyanthos’un tıbbi bitki olarak kullanılabileceğini
tahmin etmekteyiz.
Project Number
YULTEP/FEB2014/28
References
- Alam A. 2012. Some Indian Bryophytes known for their biologically active compounds. International Journal of Applied Biology and Pharmaceutical Technology. 3, 239-246.
- Asakawa Y. Ludwiczuk A. Hashimoto T. 2013a. Cytotoxic and antiviral compounds from bryophytes and inedible fungi. Journal of Pre-clinical and Clinical Research. 7:2, 73-85.
- Asakawa Y. Ludwiczuk A. Nagashima F. 2013b. Phytochemical and biological studies of bryophytes. Phytochemistry. 91, 52-80.
- Atherton I. Bosanquet S. D. Lawley M. 2010. Mosses and Liverworts of Britain And Ireland - A Field Guide. British Bryological Society. Britain.
- Burgess E. Larsen J.L.N. Perry B. 2000. A Cytotoxic sesquiterpene caffeate from the liverwort Bazzania novae-zelandiae. Journal of Natural Products. 63, 537–539.
- Chandra S. D. Chandra A. Barh A. Pankaj R. Pandey K. Sharma I. P. 2017. Bryophytes: Hoard of remedies, an ethno-medicinal review. Journal of Traditional and Complementary Medicine. 7:1, 94–98.
- Çolak E. Kara R. Ezer T. Çelik G.Y. Elibol B. 2011. Investigation of antimicrobial activity of some Turkish pleurocarpic mosses. African Journal of Biotechnology. 10, 12905–12908.
- Dey A. De J.N. 2012. Antioxidative potential of bryophytes: stress tolerance and commercial perspectives: a review. Pharmacologia. 3, 151–159.
- Dey A. Mukherjee A. 2015. Therapeutic potential of bryophytes and derived compounds against cancer. Journal of Acute Disease. 4:3, 236–248.
- Komala I.T. Ito F. Nagashima Yagi Y. Asakawa Y. 2010. Cytotoxic, radical scavenging and antimicrobial activities of sesquiterpenoids from the Tahitian liverwort Mastigophora diclados (Brid.) Nees (Mastigophoraceae). Jornal of Natural Medicines. 64, 417–422.
- Perry N. B. Foster L.M. Lorimer S.D. May B.C. Weavers R.T. 1996. Isoprenyl phenyl ethers from liverworts of the genus Trichocolea: cytotoxic activity, structural corrections and synthesis. Jornal of Natural Medicines. 59, 729-733.
- Rencüzogulları E. İla H.B. Kayraldiz A. Diler S. B. Yavuz A. Arslan M. Kaya F.F. Topaktas M. 2006. The Mutagenic and Antimutagenic Effects of Ecballium elaterium Fruit Juice in Human Peripheral Lymphocytes. Russian Journal of Genetics. 42:6, 623–627.
- Saxena D. Harinder K. 2004. Uses of Bryophytes. Resonance. 9:6, 56-65.
- Suhalka D. 2016. Eco-Friendly Management of Maize Pathogen Thruogh Bryophyte Extract. Journal of Global Pharma Technology. 8:1, 1-4.
- Toyota M. Saito T. Asakawa Y. 1999. The Absolute Configuration of Eudesmane-type Sesquiterpenoids Found in the Japanese Liverwort Chiloscyphus polyanthus. Phytochemistry. 51, 913-920.
- Xie C.F. Sun B. Guo D.X. Gao J. Qu J.B. Lou H. X. 2011. Terpenoids from the Chinese Liverwort Chiloscyphus polyanthus. Helvetica Chimica Acta. 94: 3, 534-538.
- Yağlıoğlu Ş.E. Abay G. Demirtas İ. Yağlıoğlu Ş.A. 2017. Phytochemical screening, antiproliferative and cytotoxic activities of the mosses Rhytidiadelphus triquetrus (Hedw.) Warnst. and Tortella tortuosa (Hedw.) Limpr. Anatolian Bryology 3:1, 31-42.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Project Number | YULTEP/FEB2014/28 |
| Publication Date | June 15, 2020 |
| Submission Date | October 11, 2019 |
| Published in Issue | Year 2020 Volume: 6 Issue: 1 |
