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Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits

Year 2019, Volume: 50 Issue: 1, 11 - 17, 30.01.2019
https://doi.org/10.17097/ataunizfd.413006

Abstract

Sufficient knowledge of genetic variation and germplasm classification is necessary to select suitable parents for breeding purposes. Effective and repeatable tissue culture method is a prerequisite for genetic engineering of pea genotypes (Pisum arvense L.). In this study, genetic diversity of forty-two pea genotypes (Pisum arvense L.) was evaluated based on callus induction capacity, embryogenic callus production and plant regeneration ability. Significant variation was observed among genotypes based on tissue culture parameters. The results of ANOVA indicated significant (P<0.01) differences among genotypes for traits callus induction, embryogenic callus, responded embryogenic callus, number of somatic embryos, responded somatic embryogenesis, regeneration efficiency and number of regenerated plantlet. Cluster analysis based on the tissue culture traits classified the genotypes into four groups. The highest genetic distance was observed between Subatan and Ovaçevirme-3 genotypes. The relationships among parameters related to tissue culture were investigated by principle component analysis (PCA). The PCA1 and PCA2 axes accounted 80.43% of total variation, mainly distinguish the indices in different groups.

References

  • Ahloowalia, B.S., 1982. Plant regeneration from callus culture in wheat. Crop Sci., 22:405–410.
  • Anand, I.J., Murrty, B.R., 1968. Genetic divergence and hybrid performance in linseed. Ind J Genet Plant Breed., 28: 178-185.
  • Anonymous, 2017. http://royanstore.com
  • Benadeki, S., 1992. Evaluation of genetic and geographic diversity of wheat genotypes of central region of Iran. M.Sc thesis, University of Tehran, Iran.
  • Bhatt, G.M., 1970. Multivariate analysis approach to selection of parents for hybridization aiming at yield component in self-pollination crops. Aus J Agric Rec., 21: 1-7.
  • Carman, J.G., Jefferson, N.E., Campbell, W.F., 1987. Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects. Plant Cell Tiss. Org. Cult., 10: 101–113.
  • Carves, B.F., Smith, E.L., England, H.O., 1987. Regression and cluster analysis of environmental responses of hybrid and pure line winter wheat cultivars. Crop Sci., 27: 659-664.
  • De la Vega, AJ., Chapman, S.C., 2001. Genotype by environment interaction and indirect selection for yield in sunflower: II.Three-mode principal component analysis of oil and biomass yield across environments in Argentina. Field Crops Research., 72 (1): 39-50.
  • Eivazi, A.R., Naghavi, M.R., Hajheidari, M., Pirseyedi, S.M., Ghaffari, M.R., Mohammadi, S.A., Majidi, I., Salekdeh, G.H., Mardi, M., 2007. Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Ann Appl Biol., 152:81-91.
  • Fotokian, M., Shahnejat bushehri, A., Taleie, A., 2002. Cluster analysis based on PCA in rice genotypes. Paper presented at the 6rd international conference of Statistics, University of Tarbiat modares, Iran, 26-28 August 2002.
  • Graham, P.H., Vance, C.P., 2003. Legumes: importance and constraints to greater use. Plant Physiol., 131: 872-877.
  • Griga, M.., 1998. Direct somatic embryogenesis from shoot apical meristems of pea and thidiazuron induced high conversion rate of somatic embryos. Biol. Plant., 41: 481-495.
  • Griga, M. Morphology and anatomy of Pisum sativum L. somatic embryos. Biol. Plant., 45: 173-182. 2002.
  • He, D.G., Yang, Y.M., Bertram, J., Scott, K.J., 1990. The histological development of the regenerative tissue derived from cultured immature embryos of wheat (Triticum aestivum L.). Plant Sci., 68:103-111
  • Islam, M.R., 2004. Genetic diversity in irrigated rice. Pak J Biol, Sci., 2: 226-229.
  • Joshi, A.B., Dhawan, N.L., 1966. Genetic improvement of yield with special reference to self-fertilizing crops. Ind J Genet and Plant Breed., 26: 101-113.
  • Joshi, B.K., Mudwari, A., Bhatta, M.R., Ferrara, G.O., 2004. Genetic diversity in Nepalese wheat cultivars based on agromorphological traits and coefficients of parentage. Nep Agric Res., J 5: 7-17.
  • Khodadadi, M., Fotokian, M.H., Miransari, M., 2011. Genetic diversity of wheat (Triticum aestivum L.) genotypes based on cluster and principal component analyses for breeding strategies. Aust J Crop Sci., 5(1):17-24.
  • Larkin, P.J., Scowcroft, W.R., 1981. Somaclonal variation: A novel source of variability from cell cultures for plant improvement. Theor. Appl.Genet., 60 :197–214.
  • Loridon, K., McPhee, K. Morin, J., Dubreuil, P., 2005. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor. Appl. Genet., 111: 1022-1031.
  • Maddock, S.E., Lanchester, V.A., Risiott, R., Franklin, J., 1983. Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum L.). J. Exp. Bot., 34: 915–926.
  • Maheshwari, N., Rajyalakshmi, K., Bawoja, K., Dhir, S.K., Chowdhry, C.N., Maheshwari, S.C., 1995. In vitro culture of wheat and genetic transformation – retrospect and prospect. Plant Sci., 14: 149–178
  • Mohammadi, S.A., Prasanna, B.M., 2003. Analysis of genetic diversity in crop plants: salient statical tools and considerations. Crop Sci 43; 1235-1248.Rahim, M.A., Mia, A.A., Mahmud, F., Zeba, N., Afrin, K., 2010. Genetic variability, character association and genetic divergence in Mungbean (Vigna radiate L. Wilczek). Plant Omic; 3: 1-6.

Bezelye (Pisum arvense L.) Genotiplerinin Doku Kültürü Özelliklerine Göre Genetik Çeşitliliğinin Belirlenmesi

Year 2019, Volume: 50 Issue: 1, 11 - 17, 30.01.2019
https://doi.org/10.17097/ataunizfd.413006

Abstract

Islah amaçlarına uygun ebevenyleri seçmek için gen kaynakları ve mevcut genetik çeşitlilik hakkında yeterli bilgiye sahip olmak gereklidir. Etkin ve tekrarlanabilir doku kültürü sistemi bezelye genetik mühendisliği için ön koşullardan biridir. Bu çalışmada, 42 bezelye (Pisum arvense L.) genotipinin genetik çeşitliliği kallus oluşum kapasitesi, embriyogenik kallus oluşumu ve bitki rejenerasyon yeteneğine göre değerlendirilmiştir. İstatistik analiz sonuçlarına göre; genotipler arasında, kallus oluşumu, embriyogenik kallus, cevap veren embriyojenik kallus, somatik embriyogenesis sayısı, cevap veren somatik embriyogenez, rejenerasyon etkinliği ve rejenere bitkicik sayısı özellikleri bakımından önemli (P<0.01) farklılıklar gözlenmiştir. Doku kültürü özellikleri dikkate alınarak yapılan kümeleme analizine göre genotipler 4 gruba ayrılmıştır. En yüksek genetik uzaklık Subatan ve Ovaçevirme-3 genotipleri arasında görülmüştür. Test edilen doku kültürü parametreleri arasındaki ilişkiler temel bileşenler analizi tespit edilmiş ve iki bileşenin (PCA1 ve PCA2) toplam varyasyonun %80.43’lük kısmını açıkladığı gözlenmiştir. Kallus oluşum kabiliyetinde gözlenen yüksek varyasyon, ıslah programlarında ebeveynlerin seçiminde etkin olarak kullanılabileceği sonucuna ulaşılmıştır.

References

  • Ahloowalia, B.S., 1982. Plant regeneration from callus culture in wheat. Crop Sci., 22:405–410.
  • Anand, I.J., Murrty, B.R., 1968. Genetic divergence and hybrid performance in linseed. Ind J Genet Plant Breed., 28: 178-185.
  • Anonymous, 2017. http://royanstore.com
  • Benadeki, S., 1992. Evaluation of genetic and geographic diversity of wheat genotypes of central region of Iran. M.Sc thesis, University of Tehran, Iran.
  • Bhatt, G.M., 1970. Multivariate analysis approach to selection of parents for hybridization aiming at yield component in self-pollination crops. Aus J Agric Rec., 21: 1-7.
  • Carman, J.G., Jefferson, N.E., Campbell, W.F., 1987. Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects. Plant Cell Tiss. Org. Cult., 10: 101–113.
  • Carves, B.F., Smith, E.L., England, H.O., 1987. Regression and cluster analysis of environmental responses of hybrid and pure line winter wheat cultivars. Crop Sci., 27: 659-664.
  • De la Vega, AJ., Chapman, S.C., 2001. Genotype by environment interaction and indirect selection for yield in sunflower: II.Three-mode principal component analysis of oil and biomass yield across environments in Argentina. Field Crops Research., 72 (1): 39-50.
  • Eivazi, A.R., Naghavi, M.R., Hajheidari, M., Pirseyedi, S.M., Ghaffari, M.R., Mohammadi, S.A., Majidi, I., Salekdeh, G.H., Mardi, M., 2007. Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Ann Appl Biol., 152:81-91.
  • Fotokian, M., Shahnejat bushehri, A., Taleie, A., 2002. Cluster analysis based on PCA in rice genotypes. Paper presented at the 6rd international conference of Statistics, University of Tarbiat modares, Iran, 26-28 August 2002.
  • Graham, P.H., Vance, C.P., 2003. Legumes: importance and constraints to greater use. Plant Physiol., 131: 872-877.
  • Griga, M.., 1998. Direct somatic embryogenesis from shoot apical meristems of pea and thidiazuron induced high conversion rate of somatic embryos. Biol. Plant., 41: 481-495.
  • Griga, M. Morphology and anatomy of Pisum sativum L. somatic embryos. Biol. Plant., 45: 173-182. 2002.
  • He, D.G., Yang, Y.M., Bertram, J., Scott, K.J., 1990. The histological development of the regenerative tissue derived from cultured immature embryos of wheat (Triticum aestivum L.). Plant Sci., 68:103-111
  • Islam, M.R., 2004. Genetic diversity in irrigated rice. Pak J Biol, Sci., 2: 226-229.
  • Joshi, A.B., Dhawan, N.L., 1966. Genetic improvement of yield with special reference to self-fertilizing crops. Ind J Genet and Plant Breed., 26: 101-113.
  • Joshi, B.K., Mudwari, A., Bhatta, M.R., Ferrara, G.O., 2004. Genetic diversity in Nepalese wheat cultivars based on agromorphological traits and coefficients of parentage. Nep Agric Res., J 5: 7-17.
  • Khodadadi, M., Fotokian, M.H., Miransari, M., 2011. Genetic diversity of wheat (Triticum aestivum L.) genotypes based on cluster and principal component analyses for breeding strategies. Aust J Crop Sci., 5(1):17-24.
  • Larkin, P.J., Scowcroft, W.R., 1981. Somaclonal variation: A novel source of variability from cell cultures for plant improvement. Theor. Appl.Genet., 60 :197–214.
  • Loridon, K., McPhee, K. Morin, J., Dubreuil, P., 2005. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theor. Appl. Genet., 111: 1022-1031.
  • Maddock, S.E., Lanchester, V.A., Risiott, R., Franklin, J., 1983. Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum L.). J. Exp. Bot., 34: 915–926.
  • Maheshwari, N., Rajyalakshmi, K., Bawoja, K., Dhir, S.K., Chowdhry, C.N., Maheshwari, S.C., 1995. In vitro culture of wheat and genetic transformation – retrospect and prospect. Plant Sci., 14: 149–178
  • Mohammadi, S.A., Prasanna, B.M., 2003. Analysis of genetic diversity in crop plants: salient statical tools and considerations. Crop Sci 43; 1235-1248.Rahim, M.A., Mia, A.A., Mahmud, F., Zeba, N., Afrin, K., 2010. Genetic variability, character association and genetic divergence in Mungbean (Vigna radiate L. Wilczek). Plant Omic; 3: 1-6.
There are 23 citations in total.

Details

Primary Language English
Journal Section ARAŞTIRMALAR
Authors

Parisa Bolouri This is me

Arash Hossein Pour

Gholamreza Jannati This is me

Kamil Haliloğlu

Publication Date January 30, 2019
Published in Issue Year 2019 Volume: 50 Issue: 1

Cite

APA Bolouri, P., Hossein Pour, A., Jannati, G., Haliloğlu, K. (2019). Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 50(1), 11-17. https://doi.org/10.17097/ataunizfd.413006
AMA Bolouri P, Hossein Pour A, Jannati G, Haliloğlu K. Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. January 2019;50(1):11-17. doi:10.17097/ataunizfd.413006
Chicago Bolouri, Parisa, Arash Hossein Pour, Gholamreza Jannati, and Kamil Haliloğlu. “Genetic Diversity of Pea (Pisum Arvense L.) Genotypes According to the Tissue Culture Traits”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 50, no. 1 (January 2019): 11-17. https://doi.org/10.17097/ataunizfd.413006.
EndNote Bolouri P, Hossein Pour A, Jannati G, Haliloğlu K (January 1, 2019) Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 50 1 11–17.
IEEE P. Bolouri, A. Hossein Pour, G. Jannati, and K. Haliloğlu, “Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 50, no. 1, pp. 11–17, 2019, doi: 10.17097/ataunizfd.413006.
ISNAD Bolouri, Parisa et al. “Genetic Diversity of Pea (Pisum Arvense L.) Genotypes According to the Tissue Culture Traits”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 50/1 (January 2019), 11-17. https://doi.org/10.17097/ataunizfd.413006.
JAMA Bolouri P, Hossein Pour A, Jannati G, Haliloğlu K. Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2019;50:11–17.
MLA Bolouri, Parisa et al. “Genetic Diversity of Pea (Pisum Arvense L.) Genotypes According to the Tissue Culture Traits”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 50, no. 1, 2019, pp. 11-17, doi:10.17097/ataunizfd.413006.
Vancouver Bolouri P, Hossein Pour A, Jannati G, Haliloğlu K. Genetic Diversity of Pea (Pisum arvense L.) Genotypes According to the Tissue Culture Traits. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2019;50(1):11-7.

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