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Researching germination properties of bean (Phaseolus vulgaris L.) under polyethylene glycol osmotic stress and saline conditions

Year 2020, Volume: 9 Issue: 2, 251 - 258, 30.12.2020
https://doi.org/10.29278/azd.671472

Abstract

Plant growth is negatively affected by environmental factors such as salinity and drought. This research was carried out in the laboratory to determine the germination characteristics of bean cultivar called “Akkuş” under drought and salt stress conditions. The experiment was set up using a completely randomized experimental design with ten replications. For the purpose of the study, germination rate (%), radicle length (cm), plumule length (cm), hypocotyl length (cm), radicle fresh weight (g), plumule fresh weight (g), and hypocotyl fresh weight (g) were calculated. Polyethylene glycol (PEG) 6000 was used to create a dry environment and NaCI was used for a saline environment. Different doses of NaCl (0 (control), 50, 100, 150, and 200 mM) and different osmotic potential levels of PEG 6000 (0 (control), -0.2, -0.4, -0.6, and -0.8 bar) were used in the experiment. The germination rate of bean decreased with increasing salt concentrations. Similarly, an increase in PEG 6000 osmotic potential levels caused a decrease in the parameters. The highest values were obtained in the control groups. As a result of the study, increase in salinity and drought stress cause negative effects on bean germination properties.

References

  • Chen, J., Wu, W., Zheng, Y., Hou, K., Xu, Y., Zai, J., 2010. Drought resistance of Angelica aahurica during seedling stage under polyethylene glycol (PEG-6000) simulated drought stress. China journal of Chinese Materia Medica, 35(2): 149-53.
  • Çokkızgın, A., 2012. Salinity stress in common bean (Phaseolus vulgaris L.) seed Germination. Not Bot Horti Agrobo Cluj-Napoca, 40(1): 177–182.
  • Çolak, M.S., Avcı, S., Öztürk, A., Yurtseven, E., 2018. Effects of abiotic stress conditions on plants. 1. International GAP Agriculture and Livestock Congress. 25-27 April 2018, Şanlıurfa, Turkey, 171-175.
  • Çulha, Ş., Çakırlar, H., 2011. Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe University, Journal of Sciences, 11-34.
  • Ghanbari, A.A., Mousavi, S.H., Mousapou-Gorji, A., Rao, I., 2013. Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops, 18(1): 73-77.
  • Ghiyasi, M, Tajbakhsh, M., 2013. Osmopriming alleviates drought stress in soybean (Glycine max L.) seeds during germination and early growth stages. Journal of Applied Biological Sciences. 7(1): 27-32.
  • Kalefetoğlu Macar, T., Turan, O., Ekmekci, Y., 2009. Effect of water deficit induced by PEG and NaCl on chickpea (Cicer arieitinum L.) cultivar and lines at early seedling stage. Gazi Univercity Journal of Science, 22(1): 5-14.
  • Kaya, M.D., Okçu, G., Atak, M., Çıkılı Y., Kolsarıcı Ö., 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). The European Journal of Agronomy. 24: 291–295.
  • Kaymakanova, M., 2014. Effect of salinity on germination and seed physiology in bean (Phaseolus vulgaris L.). Biotechnology & Biotechnological Equipment. Special Edition.
  • Khan, H., Ayup, A., Pervez, C.M., Bilal, M.A., Shahid, R.M., Ziaf, M.A., 2009. Effect of seed priming with NaCI on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environment, 28: 81-87.
  • Mena, E., Leiva-Mora, M., Dilhara-Jayawardana, E.K., García, L., Veitía, N., Bermúdez-Caraballoso, I., Collado, R., Cárdenas-Ortíz, R., 2015. Effect of salt stress on seed germination and seedlings growth of Phaseolus vulgaris L., Cultivos Tropicales, 36: 71-74.
  • Murillo-Amador, B., Lopez-Aguilar, R., Kaya, C., Larrinaga-Mayoral, J., Flores-Hernandez, A., 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. Journal of Agronomy and Crop Science, 188: 235-247.
  • Osakabe, Y., Arinaga. N., Umezawa, T., Katsura, S., Nagamachi, K., Tanaka, H., 2013. Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis. Plant Cell 25: 609–624.
  • Petrovic, G., Jovicic, D., Nikolic, Z., Tamindzic, G., Ignjatov, M., Milosevic, D., Milosevic, B., 2016. Comparative study of drought and salt stress effects on germination and seedling growth of pea. Genetika, 48(1): 373-381.
  • Yavas, İ., Unay, A., 2011. Evaluation of some properties for drought resistance in bread wheat. Journal of Adnan Menderes University Agricultural Faculty. 8(1): 67-72.
  • Yılmaz, E., Tuna, A.L., Bürün, B., 2011. Bitkilerin tuz stresi etkilerine karşı geliştirdikleri tolerans stratejileri. Celal Bayar Üniversitesi, Fen Bilimleri Dergisi, 7(1): 47–66.
  • Zhu, J.J.K., 2005. Understanding and improving salt tolerance in plants. Crop Science, 45: 437-448.

Polietilen glikol osmoz stresi ve tuzlu koşullar altındaki fasulye (Phaseolus vulgaris L.) bitkisinin çimlenme özelliklerinin araştırılması

Year 2020, Volume: 9 Issue: 2, 251 - 258, 30.12.2020
https://doi.org/10.29278/azd.671472

Abstract

Bitki gelişimi tuzluluk, kuraklık gibi stres faktörleri dediğimiz çevresel etmenlerden olumsuz yönde etkilenmektedir. Bu araştırma kurak ve tuzlu stres koşulları altında fasulye bitkisinin çimlenme özelliklerinin belirlenmesi amacıyla 2019 yılında Ordu Üniversitesi Tarla Bitkileri laboratuvarında yürütülmüştür. Çalışma tesadüf parselleri deneme desenine göre 10 tekrarlı olarak yürütülmüştür. Çalışmada çimlenme oranı (%), radikula uzunluğu (cm), plumula uzunluğu (cm), hipokotil uzunluğu (cm), radikula yaş ağırlığı (g), plumula yaş ağırlığı (g) ve hipokotil yaş ağırlığı (g) değerleri ölçülmüştür. Kurak ortam oluşturmak amacıyla Polietilen glikol (PEG) 6000, tuzlu ortam oluşturmak amacıyla da NaCI kullanılmıştır. Denemede 5 doz tuz (0, 50, 100, 150, 200 mM) ve 5 doz PEG ( 0, 0.2, 0.4, 0.6, 0.8 mPa ) uygulanmıştır. Artan tuz konsantrasyonları karşısında fasulye tohumlarında çimlenme oranı düşüş göstermiştir. En yüksek çimlenme oranı tuz konsantrasyonunun en düşük seviyesinde olurken, en düşük çimlenme oranı tuz konsantrasyonunun en yüksek olduğu konsantrasyonda gerçekleşmiştir. Benzer şekilde PEG dozlarında da dozlar arttıkça incelenen parametrelerde düşüşler meydana gelmiş, en yüksek ölçümler düşük dozlarda elde edilmiştir. Çalışma sonucunda tuzluluk ve kuraklık stresinin artmasıyla birlikte fasulye bitkisinde çimlenme özelliklerinde olumsuz etkiler meydana geldiği saptanmıştır.

References

  • Chen, J., Wu, W., Zheng, Y., Hou, K., Xu, Y., Zai, J., 2010. Drought resistance of Angelica aahurica during seedling stage under polyethylene glycol (PEG-6000) simulated drought stress. China journal of Chinese Materia Medica, 35(2): 149-53.
  • Çokkızgın, A., 2012. Salinity stress in common bean (Phaseolus vulgaris L.) seed Germination. Not Bot Horti Agrobo Cluj-Napoca, 40(1): 177–182.
  • Çolak, M.S., Avcı, S., Öztürk, A., Yurtseven, E., 2018. Effects of abiotic stress conditions on plants. 1. International GAP Agriculture and Livestock Congress. 25-27 April 2018, Şanlıurfa, Turkey, 171-175.
  • Çulha, Ş., Çakırlar, H., 2011. Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe University, Journal of Sciences, 11-34.
  • Ghanbari, A.A., Mousavi, S.H., Mousapou-Gorji, A., Rao, I., 2013. Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops, 18(1): 73-77.
  • Ghiyasi, M, Tajbakhsh, M., 2013. Osmopriming alleviates drought stress in soybean (Glycine max L.) seeds during germination and early growth stages. Journal of Applied Biological Sciences. 7(1): 27-32.
  • Kalefetoğlu Macar, T., Turan, O., Ekmekci, Y., 2009. Effect of water deficit induced by PEG and NaCl on chickpea (Cicer arieitinum L.) cultivar and lines at early seedling stage. Gazi Univercity Journal of Science, 22(1): 5-14.
  • Kaya, M.D., Okçu, G., Atak, M., Çıkılı Y., Kolsarıcı Ö., 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). The European Journal of Agronomy. 24: 291–295.
  • Kaymakanova, M., 2014. Effect of salinity on germination and seed physiology in bean (Phaseolus vulgaris L.). Biotechnology & Biotechnological Equipment. Special Edition.
  • Khan, H., Ayup, A., Pervez, C.M., Bilal, M.A., Shahid, R.M., Ziaf, M.A., 2009. Effect of seed priming with NaCI on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environment, 28: 81-87.
  • Mena, E., Leiva-Mora, M., Dilhara-Jayawardana, E.K., García, L., Veitía, N., Bermúdez-Caraballoso, I., Collado, R., Cárdenas-Ortíz, R., 2015. Effect of salt stress on seed germination and seedlings growth of Phaseolus vulgaris L., Cultivos Tropicales, 36: 71-74.
  • Murillo-Amador, B., Lopez-Aguilar, R., Kaya, C., Larrinaga-Mayoral, J., Flores-Hernandez, A., 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. Journal of Agronomy and Crop Science, 188: 235-247.
  • Osakabe, Y., Arinaga. N., Umezawa, T., Katsura, S., Nagamachi, K., Tanaka, H., 2013. Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis. Plant Cell 25: 609–624.
  • Petrovic, G., Jovicic, D., Nikolic, Z., Tamindzic, G., Ignjatov, M., Milosevic, D., Milosevic, B., 2016. Comparative study of drought and salt stress effects on germination and seedling growth of pea. Genetika, 48(1): 373-381.
  • Yavas, İ., Unay, A., 2011. Evaluation of some properties for drought resistance in bread wheat. Journal of Adnan Menderes University Agricultural Faculty. 8(1): 67-72.
  • Yılmaz, E., Tuna, A.L., Bürün, B., 2011. Bitkilerin tuz stresi etkilerine karşı geliştirdikleri tolerans stratejileri. Celal Bayar Üniversitesi, Fen Bilimleri Dergisi, 7(1): 47–66.
  • Zhu, J.J.K., 2005. Understanding and improving salt tolerance in plants. Crop Science, 45: 437-448.
There are 17 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Makaleler
Authors

Ferda Özkorkmaz 0000-0003-4345-9711

Nuri Yılmaz 0000-0002-0597-6884

Fatih Öner

Publication Date December 30, 2020
Published in Issue Year 2020 Volume: 9 Issue: 2

Cite

APA Özkorkmaz, F., Yılmaz, N., & Öner, F. (2020). Researching germination properties of bean (Phaseolus vulgaris L.) under polyethylene glycol osmotic stress and saline conditions. Akademik Ziraat Dergisi, 9(2), 251-258. https://doi.org/10.29278/azd.671472