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Yüksek Performanslı Sıvı Kromatografisi ile Origanum bilgeri'deki Biyoaktif Bileşiklerin Kantitatif Analizi

Year 2022, Volume: 4 Issue: 1, 15 - 20, 15.12.2022

Ramazan Erenler Esma Nur Geçer Mehmet Hakkı Alma İbrahim Demirtas

https://doi.org/10.53445/batd.1210373

Abstract

Origanum cinsi önemli biyoaktif bileşikler içeren aromatik ve tıbbi bir bitkidir. Origanum türleri yıllardır halk ilacı olarak kullanılmaktadır. Bu çalışmada, Origanum bilgeri P.H. Davis sırasıyla heksan ve metanol ile ekstrakte edildi, metanol ekstraktı, Yüksek Performanslı Sıvı Kromatografisi ile biyoaktif bileşiklerin kantitatif analizi için kullanıldı. Ana ürün olarak rosmarinik asit bulundu (26.61 mg/g ekstrakt). Diğer ana bileşikler kateşin hidrit (5,65 mg/g ekstrakt), trans-sinnamik asit (2,65 mg/g ekstrakt), resveratrol (1,33 mg/g ekstrakt) olarak tespit edildi. Sonuç olarak, Origanum bilgeri ilaç geliştirme ve gıda için değerli bir materyal olabilme potansiyeline sahiptir.

Keywords

Origanum bilgeri Doğal ürünler Kantitatif analiz

References

  • De, P., Baltas, M., & Bedos-Belval, F. (2011). Cinnamic acid derivatives as anticancer agents-a review. Current Medicinal Chemistry, 18(11), 1672-1703. doi:https://doi.org/10.2174/092986711795471347
  • Demirtas, I., Erenler, R., Elmastas, M., & Goktasoglu, A. (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry, 136(1), 34-40. doi:https://doi.org/10.1016/j.foodchem.2012.07.086
  • Elmastas, M., Celik, S. M., Genc, N., Aksit, H., Erenler, R., & Gulcin, İ. (2018). Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties, 21(1), 374-384. doi:https://doi.org/10.1080/10942912.2017.1416399
  • Elmastas, M., Ozturk, L., Gokce, I., Erenler, R., & Aboul‐Enein, H. Y. (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters, 37(9), 1859-1869. doi:https://doi.org/10.1081/AL-120039431
  • Elmastaş, M., Telci, İ., Akşit, H., & Erenler, R. (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry, 40(6), 456-462. doi:https://doi.org/10.1515/tjb-2015-0034
  • Erenler, R., Adak, T., Karan, T., Elmastas, M., Yildiz, I., Aksit, H., . . . Sanda, M. A. (2017). Chemical Constituents isolated from Origanum solymicum with Antioxidant activities. The Eurasia Proceedings of Science, Technology, Engineering & Mathematics, 1, 139-145.
  • Erenler, R., Demirtas, I., Karan, T., Gul, F., Kayir, O., & Karakoc, O. C. (2018). Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends in Phytochemical Research, 2(2), 91-96.
  • Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), 1646-1653. doi:https://doi.org/10.1080/13880209.2017.1310906
  • Erenler, R., Sen, O., Aksit, H., Demirtas, I., Yaglioglu, A. S., Elmastas, M., & Telci, İ. (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture, 96(3), 822-836. doi:https://doi.org/10.1002/jsfa.7155
  • Erenler, R., Telci, I., Ulutas, M., Demirtas, I., Gul, F., Elmastas, M., & Kayir, O. (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry, 39(5), 622-630. doi:https://doi.org/10.1111/jfbc.12168
  • Franz, C., Baser, K., & Windisch, W. (2010). Essential oils and aromatic plants in animal feeding–a European perspective. A review. Flavour and Fragrance Journal, 25(5), 327-340. doi:https://doi.org/10.1002/ffj.1967
  • Karan, T., Altuner, Z., & Erenler, R. (2017). Growth and Metabolite Production of Chroococcus minutus Under Different Temperature and Light Conditions. Journal of New Results in Science, 6(1), 47-52.
  • Karan, T., & Erenler, R. (2017). Screening of norharmane from seven cyanobacteria by high-performance liquid chromatography. Pharmacognosy Magazine, 13(Suppl 3), 723-725. doi:https://doi.org/10.4103/pm.pm_214_17
  • Karan, T., Erenler, R., & Bozer, B. M. (2022). Synthesis and characterization of silver nanoparticles using curcumin: cytotoxic, apoptotic, and necrotic effects on various cell lines. Zeitschrift für Naturforschung C, 77(7-8), 343-350. doi:https://doi.org/10.1515/znc-2021-0298
  • Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, Article ID 162750. doi:https://doi.org/10.1155/2013/162750
  • Lin, L.-Z., Mukhopadhyay, S., Robbins, R. J., & Harnly, J. M. (2007). Identification and quantification of flavonoids of Mexican oregano (Lippia graveolens) by LC-DAD-ESI/MS analysis. Journal of food composition and analysis, 20(5), 361-369. Petersen, M., & Simmonds, M. S. (2003). Rosmarinic acid. Phytochemistry, 62(2), 121-125. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12482446
  • Phillips, I. (2007). Withdrawal of growth-promoting antibiotics in Europe and its effects in relation to human health. International journal of antimicrobial agents, 30(2), 101-107. doi:https://doi.org/10.1016/j.ijantimicag.2007.02.018 Saidi, A., Hambaba, L., Kucuk, B., Cacan, E., & Erenler, R. (2022). Phenolic Profile, Acute Toxicity, and Hepatoprotective and Antiproliferative Activities of Algerian Ruta tuberculata Forssk. Current Bioactive Compounds, 18(3), 72-83. doi:https://doi.org/10.2174/1573407217666211119092552
  • Topçu, G., Erenler, R., Çakmak, O., Johansson, C. B., Çelik, C., Chai, H.-B., & Pezzuto, J. M. (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry, 50(7), 1195-1199. doi:https://doi.org/10.1016/S0031-9422(98)00675-X Türkmen, N., Öz, A., Sönmez, A., Erol, T., Gülümser, D., Yurdakul, B., . . . Erenler, R. (2014). Chemical Composition of Essential Oil from Rosmarinus Officinalis L. Leaves. Journal of New Results in Science, 6(6), 27-31.
  • Zhang, X.-L., Guo, Y.-S., Wang, C.-H., Li, G.-Q., Xu, J.-J., Chung, H. Y., . . . Wang, G.-C. (2014). Phenolic compounds from Origanum vulgare and their antioxidant and antiviral activities. Food Chemistry, 152, 300-306. doi:https://doi.org/10.1016/j.foodchem.2013.11.153

Quantitative Analysis of Bioactive Compounds by High‑Performance Liquid Chromatography in Origanum bilgeri

Year 2022, Volume: 4 Issue: 1, 15 - 20, 15.12.2022

Ramazan Erenler Esma Nur Geçer Mehmet Hakkı Alma İbrahim Demirtas

https://doi.org/10.53445/batd.1210373

Abstract

Origanum genus is an aromatic and medicinal plant with significant bioactive compounds. Origanum species have been used as a folk medicine for years. In this study, the aerial part of Origanum bilgeri P.H. Davis was extracted with hexane and methanol successively, the methanol extract was used for quantitative analysis of bioactive compounds by High‑Performance Liquid Chromatography. Rosmarinic acid was found as a major product (26.61 mg/g extract). The other major compounds were detected as catechin hydride (5.65 mg/g extract), trans-cinnamic acid (2.65 mg/g extract), and resveratrol (1.33 mg/g extract). Consequently, Origanum bilgeri may be a valuable material for drug development and food.

Keywords

Origanum bilgeri Natural products Quantitative analysis

References

  • De, P., Baltas, M., & Bedos-Belval, F. (2011). Cinnamic acid derivatives as anticancer agents-a review. Current Medicinal Chemistry, 18(11), 1672-1703. doi:https://doi.org/10.2174/092986711795471347
  • Demirtas, I., Erenler, R., Elmastas, M., & Goktasoglu, A. (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry, 136(1), 34-40. doi:https://doi.org/10.1016/j.foodchem.2012.07.086
  • Elmastas, M., Celik, S. M., Genc, N., Aksit, H., Erenler, R., & Gulcin, İ. (2018). Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties, 21(1), 374-384. doi:https://doi.org/10.1080/10942912.2017.1416399
  • Elmastas, M., Ozturk, L., Gokce, I., Erenler, R., & Aboul‐Enein, H. Y. (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters, 37(9), 1859-1869. doi:https://doi.org/10.1081/AL-120039431
  • Elmastaş, M., Telci, İ., Akşit, H., & Erenler, R. (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry, 40(6), 456-462. doi:https://doi.org/10.1515/tjb-2015-0034
  • Erenler, R., Adak, T., Karan, T., Elmastas, M., Yildiz, I., Aksit, H., . . . Sanda, M. A. (2017). Chemical Constituents isolated from Origanum solymicum with Antioxidant activities. The Eurasia Proceedings of Science, Technology, Engineering & Mathematics, 1, 139-145.
  • Erenler, R., Demirtas, I., Karan, T., Gul, F., Kayir, O., & Karakoc, O. C. (2018). Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends in Phytochemical Research, 2(2), 91-96.
  • Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), 1646-1653. doi:https://doi.org/10.1080/13880209.2017.1310906
  • Erenler, R., Sen, O., Aksit, H., Demirtas, I., Yaglioglu, A. S., Elmastas, M., & Telci, İ. (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture, 96(3), 822-836. doi:https://doi.org/10.1002/jsfa.7155
  • Erenler, R., Telci, I., Ulutas, M., Demirtas, I., Gul, F., Elmastas, M., & Kayir, O. (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry, 39(5), 622-630. doi:https://doi.org/10.1111/jfbc.12168
  • Franz, C., Baser, K., & Windisch, W. (2010). Essential oils and aromatic plants in animal feeding–a European perspective. A review. Flavour and Fragrance Journal, 25(5), 327-340. doi:https://doi.org/10.1002/ffj.1967
  • Karan, T., Altuner, Z., & Erenler, R. (2017). Growth and Metabolite Production of Chroococcus minutus Under Different Temperature and Light Conditions. Journal of New Results in Science, 6(1), 47-52.
  • Karan, T., & Erenler, R. (2017). Screening of norharmane from seven cyanobacteria by high-performance liquid chromatography. Pharmacognosy Magazine, 13(Suppl 3), 723-725. doi:https://doi.org/10.4103/pm.pm_214_17
  • Karan, T., Erenler, R., & Bozer, B. M. (2022). Synthesis and characterization of silver nanoparticles using curcumin: cytotoxic, apoptotic, and necrotic effects on various cell lines. Zeitschrift für Naturforschung C, 77(7-8), 343-350. doi:https://doi.org/10.1515/znc-2021-0298
  • Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, Article ID 162750. doi:https://doi.org/10.1155/2013/162750
  • Lin, L.-Z., Mukhopadhyay, S., Robbins, R. J., & Harnly, J. M. (2007). Identification and quantification of flavonoids of Mexican oregano (Lippia graveolens) by LC-DAD-ESI/MS analysis. Journal of food composition and analysis, 20(5), 361-369. Petersen, M., & Simmonds, M. S. (2003). Rosmarinic acid. Phytochemistry, 62(2), 121-125. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12482446
  • Phillips, I. (2007). Withdrawal of growth-promoting antibiotics in Europe and its effects in relation to human health. International journal of antimicrobial agents, 30(2), 101-107. doi:https://doi.org/10.1016/j.ijantimicag.2007.02.018 Saidi, A., Hambaba, L., Kucuk, B., Cacan, E., & Erenler, R. (2022). Phenolic Profile, Acute Toxicity, and Hepatoprotective and Antiproliferative Activities of Algerian Ruta tuberculata Forssk. Current Bioactive Compounds, 18(3), 72-83. doi:https://doi.org/10.2174/1573407217666211119092552
  • Topçu, G., Erenler, R., Çakmak, O., Johansson, C. B., Çelik, C., Chai, H.-B., & Pezzuto, J. M. (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry, 50(7), 1195-1199. doi:https://doi.org/10.1016/S0031-9422(98)00675-X Türkmen, N., Öz, A., Sönmez, A., Erol, T., Gülümser, D., Yurdakul, B., . . . Erenler, R. (2014). Chemical Composition of Essential Oil from Rosmarinus Officinalis L. Leaves. Journal of New Results in Science, 6(6), 27-31.
  • Zhang, X.-L., Guo, Y.-S., Wang, C.-H., Li, G.-Q., Xu, J.-J., Chung, H. Y., . . . Wang, G.-C. (2014). Phenolic compounds from Origanum vulgare and their antioxidant and antiviral activities. Food Chemistry, 152, 300-306. doi:https://doi.org/10.1016/j.foodchem.2013.11.153
There are 19 citations in total.

Details

Primary Language English
Subjects Analytical Chemistry, Chemical Engineering
Journal Section Research Articles
Authors

Ramazan Erenler 0000-0002-0505-3190

Esma Nur Geçer 0000-0002-0095-079X

Mehmet Hakkı Alma 0000-0001-6323-7230

İbrahim Demirtas 0000-0001-8946-647X

Publication Date December 15, 2022
Published in Issue Year 2022 Volume: 4 Issue: 1

Cite

APA Erenler, R., Geçer, E. N., Alma, M. H., Demirtas, İ. (2022). Quantitative Analysis of Bioactive Compounds by High‑Performance Liquid Chromatography in Origanum bilgeri. Bütünleyici Ve Anadolu Tıbbı Dergisi, 4(1), 15-20. https://doi.org/10.53445/batd.1210373
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Bütünleyici ve Anadolu Tıbbı Dergisi

Journal of Integrative and Anatolian Medicine