Kurkumin’in U251 hücrelerinde antineoplastik potansiyellerinin mekanizması olarak anti-oksidatif ve apoptotik özellikleri
Abstract
Glioblastoma beyin kanser türleri arasında en agresif olanıdır. Özellikle kanser araştırmalarında in vitro çalışmalar ile yeni ilaç adaylarının etkinliği araştırılmaktadır. Bu çalışma ile U251 hücreleri üzerinde farklı dozlarda kurkumin uygulamasının antineoplastik etkilerini araştırmayı amaçladık. Kurkumin’in, prooksidan-antioksidan mekanizmaları modüle ederek ROS üretimini, apoptoz aktivitesini ve inflamasyonu tetikleyerek U251 hücre canlılığını inhibe ettiğini varsayıyoruz. Bu çalışmada, IL-1β, TNF-α, kaspaz 3/9 seviyeleri, toplam antioksidan (TAS) ve toplam oksidan seviyeleri (TOS) ölçüldü. MTT ile hücre canlılıkları belirlendi ve 10, 20 ve 40 µM kurkumin dozlarının efektif dozlar olduğu tespit edildi. 40 µM kurkumin uygulamasının kontrol grubu ve diğer doz grupları ile karşılaştırıldığında oksidatif stresi, inflamasyonu ve apoptozu indüklediği bulundu (p<0.05). 10 µM kurkumin grubunda istatistiksel olarak fark tespit edilmedi. 40 µM kurkuminin U251 glioblastoma hücrelerinin proliferasyonu ve migrasyonu üzerinde doza bağlı bir etkiye sahip olduğunu ve proliferasyonunu önemli ölçüde inhibe ettiğini bulduk
Keywords
References
- [1] Ostrom, Q. T., Bauchet, L., Davis, F. G., Deltour, I., Fisher, J. L., Langer, C. E., ... & Barnholtz-Sloan, J. S. (2014). The epidemiology of glioma in adults: a “state of the science” review. Neuro-oncology, 16(7), 896-913.
- [2] Vitovcova, B., Skarkova, V., Rudolf, K., & Rudolf, E. (2020). Biology of Glioblastoma Multiforme—Exploration of Mitotic Catastrophe as a Potential Treatment Modality. International Journal of Molecular Sciences, 21(15), 5324.
Anti-oxidative and apoptotic properties of curcumin as a mechanism of its antineoplastic potential in U251 cells
Abstract
Glioblastoma is the most aggressive of all types of brain cancer. Especially in cancer research, the effectiveness of new drug candidates is being investigated with in vitro studies. In this study, we aimed to investigate the antineoplastic effects of curcumin administration at different doses on U251 cells. We hypothesize that curcumin inhibits U251 cell viability by modulating prooxidant-antioxidant mechanisms, triggering ROS production, apoptosis activity, and inflammation. In this study, IL-1β, TNF-α, caspase 3/9 levels, total antioxidant (TAS) and total oxidant levels (TOS) were measured. Cell viability was determined by MTT and 10, 20 and 40 µM curcumin doses were found to be effective doses. It was found that the administration of 40 µM curcumin induced oxidative stress, inflammation and apoptosis compared to the control group and other dose groups (p<0.05). No statistical difference was found in the 10 µM curcumin group. We found that 40 µM curcumin had a dose-dependent effect on proliferation and migration of U251 glioblastoma cells and significantly inhibited their proliferation.
Keywords
References
- [1] Ostrom, Q. T., Bauchet, L., Davis, F. G., Deltour, I., Fisher, J. L., Langer, C. E., ... & Barnholtz-Sloan, J. S. (2014). The epidemiology of glioma in adults: a “state of the science” review. Neuro-oncology, 16(7), 896-913.
- [2] Vitovcova, B., Skarkova, V., Rudolf, K., & Rudolf, E. (2020). Biology of Glioblastoma Multiforme—Exploration of Mitotic Catastrophe as a Potential Treatment Modality. International Journal of Molecular Sciences, 21(15), 5324.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Biochemistry and Cell Biology (Other) |
| Journal Section | Research Article |
| Authors | |
| Publication Date | August 15, 2021 |
| Submission Date | June 28, 2021 |
| Acceptance Date | July 21, 2021 |
| Published in Issue | Year 2021 Volume: 14 Issue: 2 |
❖ Abstracted-Indexed in
Web of Science {Zoological Records Indexed] Clavariate Analytic, Medical Reads (RRS), CrossRef;10.46309/biodicon.
❖ Libraries
Aberystwyth University; All libraries; Bath University; Birmingham University; Cardiff University; City University London; CONSER (Not UK Holdings); Edinburgh University; Essex University; Exeter University; Eskişehir Technical University Library; EZB Electronic Journals Library; Feng Chia University Library; GAZİ Gazi University Library; Glasgow University; HEC-National Digital Library; Hull University; Imperial College London; Kaohsinug Medical University Library; ANKOS; Anadolu University Library; Lancaster University; Libros PDF; Liverpool University; London Metropolitan University; London School of Economics and Political Science; Manchester University; National Cheng Kung University Library; National ILAN University Library; Nottingham University; Open University; Oxford University; Queen Mary,University of London;Robert Gordon University; Royal Botanic Gardens, Kew; Sheffield Hallam University; Sheffield University; Shih Hsin University Library; Smithsonian Institution Libraries; Southampton University; Stirling University; Strathclyde University; Sussex University; The National Agricultural Library (NAL); The Ohio Library and Information NetWork; Trinity College Dublin; University of Washington Libraries; Vaughan Memorial Library; York University..
❖ The article processing is free.
❖ Web of Science-Clarivate Analytics, Zoological Record
❖ This journal is a CrossRef;10.46309/biodicon. member
❖ Please visit ” http:// www.biodicon.com“ ; "https://dergipark.org.tr/en/pub/biodicon" for instructions about articles and all of the details about journal
❖ Correspondance Adres: Prof. Ersin YÜCEL, Sazova Mahallesi, Ziraat Caddesi, No.277 F Blok, 26005 Tepebaşı-Eskişehir/Türkiye
❖ E-posta / E-mail: biodicon@gmail.com;
❖ Web Address: http://www.biodicon.com; https://dergipark.org.tr/en/pub/biodicon
❖ Biological Diversity and Conservation/ Biyolojik Çeşitlilik ve Koruma
❖ ISSN 1308-5301 Print; ISSN 1308-8084 Online
❖ Start Date Published 2008
❖ © Copyright by Biological Diversity and Conservation/Biyolojik Çeşitlilik ve Koruma-Available online at www.biodicon.com/All rights reserved
❖ Publisher : ERSİN YÜCEL (https://www.ersinyucel.com.tr/)
❖ This journal is published three numbers in a year. Printed in Eskişehir/Türkiye.
❖ All sorts of responsibilities of the articles published in this journal are belonging to the authors
❖ Editör / Editor-In-Chief : Prof.Dr. Ersin YÜCEL, https://orcid.org/0000-0001-8274-7578