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Year 2020, Volume: 1 Issue: 2, 39 - 42, 30.12.2020

Merve Şifa Hane Köse Muhammet Doğan Gökhan Sadi

Abstract

References

  • Ahmad S, Jakhar ML, Limenie AD, Ram M, Mtilimbanya KY, Jat HR (2020) Systematic review on micropropagation of Aloe barbadensis Mill a medicinal lily of desert. J Pharmacogn Phytochem 9(2):801-804
  • Al Ghasheem N, Stănică F, Peticilă AG, Venat O (2018) In vitro effect of various sterilization techniques on peach (Prunus persica (L.) Batsch) explants. Sci Papers 63:227-234.
  • Boedeltje G, Ozinga WA, Prinzing A (2008) The trade‐off between vegetative and generative reproduction among angiosperms influences regional hydrochorous propagule pressure. Global Ecol Biogeogr 17(1):50-58
  • Carvalho PPD, Antoniazzi CA, Faria RBD, Carvalho IFD, Rocha DI, Silva MLD (2019) In vitro organogenesis from root explants of Passiflora miniata Mast, an amazonian species with ornamental potential. Braz Arch Biol Technol 62:e19170803
  • Chen LR, Hsiung TC, Lin KH, Huang TB, Huang MY, Wakana A (2017) Supplementary effect of hydrogen peroxide as a pre–disinfectant for sterilizing rhizome bud explants of Zantedeschia aethiopica L. with chlorine dioxide. J Fac Agr Kyushu U 62(1):81-86
  • Dogan M (2018) In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech 8:77-84
  • Dogan, M (2019) In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Ant J Bot 3(1), 1-6
  • Gupta N, Jain V, Joseph MR, Devi S (2020) A review on micropropagation culture method. Asian J Pharm Res 8(1):86-93
  • Gutiérrez B, Cobo MM, Orellana M, Vega J, Arahana V, Jaramillo V, de Lourdes Torres M (2019) Micropropagation of Solanum quitoense var. quitoense by apical bud, petiole and hypocotyl culture. Plant Biotechnol 36(2):91-97
  • Halim MA, Alam MF, Rahman MH, Hossain MB, Uddin MB (2016) Sterilization process for in vitro regeneration of Stevia (Stevia rebundiana Bertoni). Int J Bus 4(4):320-323
  • Javed SB, Alatar AA, Basahi R, Anis M, Faisal M, Husain FM (2017) Copper induced suppression of systemic microbial contamination in Erythrina variegata L. during in vitro culture. Plant Cell Tiss Org Cult 128(2):249-258
  • Klaocheed S, Jehsu W, Choojun W, Thammasiri K, Prasertsongskun S, Rittirat S (2020) Induction of direct shoot organogenesis from shoot tip explants of an ornamental aquatic plant, Cryptocoryne wendtii. Walailak J Sci & Tech 17(4)
  • Koike I, Shimomura K, Umehara M (2018) Quantification of endogenous auxin and cytokinin during internode culture of ipecac. J Vis Exp 133:e56902
  • Mahmoud SN, Al-Ani NK (2016) Effect of different sterilization methods on contamination and viability of nodal segments of Cestrum nocturnum L. Int J Res Stud Biosci 4(1):4-9
  • Mandeh M, Omidi M, Rahaie M (2012) In vitro influences of TiO2 nanoparticles on barley (Hordeum vulgare L.) tissue culture. Biol Trace Elem Res 150(1-3):376-380
  • McCall AG (1915) A new method for the study of plant nutrients in sand cultures 1. J Agron 7(5):249-252
  • Metwaly A, Salama GM, Ali GA (2018) Using hydrogen peroxide for reducing bacterial contamination in date palm tissue culture. Int J Adv Agric Sci Technol 5:25-33
  • Mishra AK, Tiwari KN, Mishra P, Tiwari SK, Mishra SK, Saini R (2019) Effect of cytokinin and MS medium composition on efficient shoot proliferation of Nyctanthes arbor-tristis L. through cotyledonary node explant and evaluation of genetic fidelity and antioxidant capacity of regenerants. S Afr J Bot 127:284-292
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473-497
  • Orlikowska T, Nowak K, Reed B (2017) Bacteria in the plant tissue culture environment. Plant Cell Tiss Org Cult 128(3):487-508
  • Oyebanji OB, Nweke O, Odebunmi O, Galadima NB, Idris MS, Nnodi UN, Afolabi AS, Ogbadu GH (2009) Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds. Afr J Biotechnol 8(20):5395-5399.
  • Pathak A, Joshi A, Sharma A (2019) Development of shoot cultures from leaf explant of Portulaca quadrifida L. Not Sci Biol 11(1):45-50
  • Phillips GC, Garda M (2019) Plant tissue culture media and practices: an overview. In Vitro Cell Dev Biol Plant 55(3):242-257
  • Priyadarshan PM (2019) Tissue Culture. In Plant Breeding: Classical to Modern (pp. 475-491). Springer, Singapore
  • Rojsanga P, Bunsupa S, Sithisarn P (2020) Flavones contents in extracts from Oroxylum indicum seeds and plant tissue cultures. Molecules 25(7):1545
  • Sax MS, Bassuk N, Bridgen M (2019) Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment. HortScience 54(12):2214-2223
  • Sereda MM, Lutsenko EV, Chokheli VA, Vereschagina AV, Rachkovskaya KY, Lysenko VS. Varduny TV (2017) A method for microclonal propagation of Staurogyne repens in tissue culture. J Plant Sci 12(1):17-21
  • Shukla S, Singh M, Kumar A, Rajbhar Y, Kumar M, Singh SP (2019) Effects of surface sterilization agents under in vitro culture of banana (Musa paradisiaca L.) variety “Udhayam”. J Pharmacogn Phytochem 8(1):843-846
  • Singh G, Gupta S (2019) Evaluation of sterilant effect on in-vitro culture establishment in sugarcane variety Co 0118. Int J Curr Microbiol App Sci 8(7):1226-1233
  • Sirimat S, Sakulsathaporn A (2019) Optimization of explant surface sterilization conditions and multiple shoot ınduction in threatened plant Phanera sirindhorniae. J Adv Agric Technol 6(4):263-266
  • Vandana S, Ashwini K, Arun K, Sumit K (2020) An efficient in vitro propagation protocol for Catharanthus roseus (L.). Res J Biotechnol 15:1

Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide

Year 2020, Volume: 1 Issue: 2, 39 - 42, 30.12.2020

Merve Şifa Hane Köse Muhammet Doğan Gökhan Sadi

Abstract

Sterilization is the killing, destruction or removal of all forms of microorganisms with a substance or an object. In tissue culture studies, the environment and equipment are sterilized. Likewise, the plant to be produced must be sterilized. It uses many methods and chemicals for the surface sterilization of plants. In this study, surface sterilization of Staurogyne repens (Nees) Kuntze was investigated using hydrogen peroskite (H2O2) at different times (10-30 min) and concentrations (3.7- 7.4% v/v). Nodal explants were used in trials. No plant growth regulator was added to the culture medium. Contaminations in food media started to be observed after 5 days and all data were collected after four weeks. While bacterial contaminations were recorded, a few fungal contaminations were also observed. High levels of H2O2 negatively affected the regeneration capabilities of explants. Also, some explants died due to H2O2. Contamination percentages were recorded between 40-100% in H2O2 applied environments. The highest sterilization rate (25%) were obtained in explants exposed to 5.5% H2O2 for 20 min. It was then recorded on explants treated with 5.5% H2O2 for 30 minutes (20%). As a result, surface sterilization of S. repens was accomplished using H2O2. These results can be helpful for surface sterilization and tissue culture studies of S.repens.

Keywords

Contamination nodal explan sterilization tissue culture

References

  • Ahmad S, Jakhar ML, Limenie AD, Ram M, Mtilimbanya KY, Jat HR (2020) Systematic review on micropropagation of Aloe barbadensis Mill a medicinal lily of desert. J Pharmacogn Phytochem 9(2):801-804
  • Al Ghasheem N, Stănică F, Peticilă AG, Venat O (2018) In vitro effect of various sterilization techniques on peach (Prunus persica (L.) Batsch) explants. Sci Papers 63:227-234.
  • Boedeltje G, Ozinga WA, Prinzing A (2008) The trade‐off between vegetative and generative reproduction among angiosperms influences regional hydrochorous propagule pressure. Global Ecol Biogeogr 17(1):50-58
  • Carvalho PPD, Antoniazzi CA, Faria RBD, Carvalho IFD, Rocha DI, Silva MLD (2019) In vitro organogenesis from root explants of Passiflora miniata Mast, an amazonian species with ornamental potential. Braz Arch Biol Technol 62:e19170803
  • Chen LR, Hsiung TC, Lin KH, Huang TB, Huang MY, Wakana A (2017) Supplementary effect of hydrogen peroxide as a pre–disinfectant for sterilizing rhizome bud explants of Zantedeschia aethiopica L. with chlorine dioxide. J Fac Agr Kyushu U 62(1):81-86
  • Dogan M (2018) In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech 8:77-84
  • Dogan, M (2019) In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Ant J Bot 3(1), 1-6
  • Gupta N, Jain V, Joseph MR, Devi S (2020) A review on micropropagation culture method. Asian J Pharm Res 8(1):86-93
  • Gutiérrez B, Cobo MM, Orellana M, Vega J, Arahana V, Jaramillo V, de Lourdes Torres M (2019) Micropropagation of Solanum quitoense var. quitoense by apical bud, petiole and hypocotyl culture. Plant Biotechnol 36(2):91-97
  • Halim MA, Alam MF, Rahman MH, Hossain MB, Uddin MB (2016) Sterilization process for in vitro regeneration of Stevia (Stevia rebundiana Bertoni). Int J Bus 4(4):320-323
  • Javed SB, Alatar AA, Basahi R, Anis M, Faisal M, Husain FM (2017) Copper induced suppression of systemic microbial contamination in Erythrina variegata L. during in vitro culture. Plant Cell Tiss Org Cult 128(2):249-258
  • Klaocheed S, Jehsu W, Choojun W, Thammasiri K, Prasertsongskun S, Rittirat S (2020) Induction of direct shoot organogenesis from shoot tip explants of an ornamental aquatic plant, Cryptocoryne wendtii. Walailak J Sci & Tech 17(4)
  • Koike I, Shimomura K, Umehara M (2018) Quantification of endogenous auxin and cytokinin during internode culture of ipecac. J Vis Exp 133:e56902
  • Mahmoud SN, Al-Ani NK (2016) Effect of different sterilization methods on contamination and viability of nodal segments of Cestrum nocturnum L. Int J Res Stud Biosci 4(1):4-9
  • Mandeh M, Omidi M, Rahaie M (2012) In vitro influences of TiO2 nanoparticles on barley (Hordeum vulgare L.) tissue culture. Biol Trace Elem Res 150(1-3):376-380
  • McCall AG (1915) A new method for the study of plant nutrients in sand cultures 1. J Agron 7(5):249-252
  • Metwaly A, Salama GM, Ali GA (2018) Using hydrogen peroxide for reducing bacterial contamination in date palm tissue culture. Int J Adv Agric Sci Technol 5:25-33
  • Mishra AK, Tiwari KN, Mishra P, Tiwari SK, Mishra SK, Saini R (2019) Effect of cytokinin and MS medium composition on efficient shoot proliferation of Nyctanthes arbor-tristis L. through cotyledonary node explant and evaluation of genetic fidelity and antioxidant capacity of regenerants. S Afr J Bot 127:284-292
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473-497
  • Orlikowska T, Nowak K, Reed B (2017) Bacteria in the plant tissue culture environment. Plant Cell Tiss Org Cult 128(3):487-508
  • Oyebanji OB, Nweke O, Odebunmi O, Galadima NB, Idris MS, Nnodi UN, Afolabi AS, Ogbadu GH (2009) Simple, effective and economical explant-surface sterilization protocol for cowpea, rice and sorghum seeds. Afr J Biotechnol 8(20):5395-5399.
  • Pathak A, Joshi A, Sharma A (2019) Development of shoot cultures from leaf explant of Portulaca quadrifida L. Not Sci Biol 11(1):45-50
  • Phillips GC, Garda M (2019) Plant tissue culture media and practices: an overview. In Vitro Cell Dev Biol Plant 55(3):242-257
  • Priyadarshan PM (2019) Tissue Culture. In Plant Breeding: Classical to Modern (pp. 475-491). Springer, Singapore
  • Rojsanga P, Bunsupa S, Sithisarn P (2020) Flavones contents in extracts from Oroxylum indicum seeds and plant tissue cultures. Molecules 25(7):1545
  • Sax MS, Bassuk N, Bridgen M (2019) Tissue Culture Clonal Propagation of Hybrid White Oaks for the Urban Environment. HortScience 54(12):2214-2223
  • Sereda MM, Lutsenko EV, Chokheli VA, Vereschagina AV, Rachkovskaya KY, Lysenko VS. Varduny TV (2017) A method for microclonal propagation of Staurogyne repens in tissue culture. J Plant Sci 12(1):17-21
  • Shukla S, Singh M, Kumar A, Rajbhar Y, Kumar M, Singh SP (2019) Effects of surface sterilization agents under in vitro culture of banana (Musa paradisiaca L.) variety “Udhayam”. J Pharmacogn Phytochem 8(1):843-846
  • Singh G, Gupta S (2019) Evaluation of sterilant effect on in-vitro culture establishment in sugarcane variety Co 0118. Int J Curr Microbiol App Sci 8(7):1226-1233
  • Sirimat S, Sakulsathaporn A (2019) Optimization of explant surface sterilization conditions and multiple shoot ınduction in threatened plant Phanera sirindhorniae. J Adv Agric Technol 6(4):263-266
  • Vandana S, Ashwini K, Arun K, Sumit K (2020) An efficient in vitro propagation protocol for Catharanthus roseus (L.). Res J Biotechnol 15:1
There are 31 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Merve Şifa Hane Köse

Muhammet Doğan

Gökhan Sadi This is me

Publication Date December 30, 2020
Acceptance Date September 10, 2020
Published in Issue Year 2020 Volume: 1 Issue: 2

Cite

APA Köse, M. Ş. H., Doğan, M., & Sadi, G. (2020). Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide. Bulletin of Biotechnology, 1(2), 39-42.
AMA Köse MŞH, Doğan M, Sadi G. Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide. Bull. Biotechnol. December 2020;1(2):39-42.
Chicago Köse, Merve Şifa Hane, Muhammet Doğan, and Gökhan Sadi. “Surface Sterilization of Staurogyne Repens (Nees) Kuntze With Hydrogen Peroxide”. Bulletin of Biotechnology 1, no. 2 (December 2020): 39-42.
EndNote Köse MŞH, Doğan M, Sadi G (December 1, 2020) Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide. Bulletin of Biotechnology 1 2 39–42.
IEEE M. Ş. H. Köse, M. Doğan, and G. Sadi, “Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide”, Bull. Biotechnol., vol. 1, no. 2, pp. 39–42, 2020.
ISNAD Köse, Merve Şifa Hane et al. “Surface Sterilization of Staurogyne Repens (Nees) Kuntze With Hydrogen Peroxide”. Bulletin of Biotechnology 1/2 (December 2020), 39-42.
JAMA Köse MŞH, Doğan M, Sadi G. Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide. Bull. Biotechnol. 2020;1:39–42.
MLA Köse, Merve Şifa Hane et al. “Surface Sterilization of Staurogyne Repens (Nees) Kuntze With Hydrogen Peroxide”. Bulletin of Biotechnology, vol. 1, no. 2, 2020, pp. 39-42.
Vancouver Köse MŞH, Doğan M, Sadi G. Surface sterilization of Staurogyne repens (Nees) Kuntze with hydrogen peroxide. Bull. Biotechnol. 2020;1(2):39-42.
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