Research Article
BibTex RIS Cite

Tekirdağ ilinde kirazda Bakteriyel kanser hastalığına neden olan hastalık etmenlerinin karakterizasyonu

Year 2016, Volume: 56 Issue: 4, 385 - 397, 31.12.2016

Abstract



Kiraz (Prunus avium L.) Tekirdağ’da yetiştirilen önemli bir meyve
türüdür. Kirazda verim ve
kaliteyi düşüren, ağaçları kurutan bakteriyel
kanser hastalığı etmen(ler)i bu çalışma ile
araştırılmıştır. Tekirdağ ili kiraz bahçelerinde 2012-2013 yıllarında gerçekleştirilen sürvey
araştırmaları sonucu 150 hasta bitki örneği
belirti gösteren ağaçlardan toplanmıştır. Tekirdağ ilinde
surveye dahil edilen bahçelerin
tümünde hastalığın görüldüğü hastalık oranının %20-57, hastalık şiddetinin ise %20-85 oranında
olduğu tespit edilmiştir. Laboratuvarda yapılan
izolasyon çalışmaları sonucu, bu örneklerden 60 adet Pseudomonas syringae
izolatı elde edilmiştir. Yapılan
LOPAT testleri sonucunda izolatlar oksidaz, pektolitik aktivite, arginin
dehidrolaz negatif, tütünde aşırı duyarlılık ve levan pozitif olarak
değerlendirilmiştir. Test edilen
izolatların GATTa özellikleri, Pseudomonas syringae pv. syringae
olarak tanılanan 32 izolat için (+
+- -) olarak kaydedilirken
Pseudomonas syringae pv. morsprunorum olarak tanılanan 28 izolat için (- -+ +) olarak bulunmuştur. Tesadüfen seçilen izolatlar yağ
asidi analizlerine göre %62-90 oranında benzerlik göstermiştir. Moleküler
testlerde kullanılan spesifik primerler ile 28 adet izolat 650 bp
tekrarlanabilir bant oluşturarak P. syringae pv. morsprunorum ve
32 adet izolat 752 bp tekrarlanabilir bant oluşturması nedeniyle P. syringae
pv. syringae olarak tanılanmıştır.




References

  • Abbasi V., Rahimian H. and Tajick-Ghanbari M.A. 2013. Genetic variability of Iranian strains of Pseudomonas syringae pv. syringae causing bacterial canker disease of stone fruits. European Journal of Plant Pathology, 135, 225-235.

Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province

Year 2016, Volume: 56 Issue: 4, 385 - 397, 31.12.2016

Abstract



Sweet cherry (Prunus
avium
L.) is one of the most important fruit trees grown in Tekirdağ.
Causal disease agent(s) of bacterial canker which
reduces yield and quality of sweet cherry fruit and cause death of trees were
investigated. For this purpose a survey study was conducted in 2012-2013 and
150 infected plant samples were collected from symptomatic trees. As a result
of survey studies, bacterial canker was determined in all orchards, while the
prevalence
rate of diseases was determined as 20-57%
and its severity estimated as 20-85%
in
surveyed orchards. As a result of isolation studies, 60 bacterial isolates of
Pseudomonas syringae were obtained.
Results obtained from LOPAT, oxidase, pectolytic activity and arginine
dihydrolase revealed that all isolates were recorded as negative for oxidase,
pectolytic activity and arginine dihydrolase, but were positive on tobacco and
levan production. Further GATTa characters of tested isolates were found as (+
+ - -) for 28 isolates identified as
Pseudomonas syringae pv. morsprunorum, while
32 isolates were recorded as (- - + +), identified as
Pseudomonas
syringae
pv. syringae.
Coincidentally 62-90% of selected isolates showed similarity according to fatty
asit methyl ester analaysis. By using specific primers, 28 isolates formed 650
bp repeatable band so identified as
Pseudomonas syringae pv. morsprunorum,
32 isolates formed 752 bp repeatable
band so identified as
Pseudomonas syringae pv. syringae.




References

  • Abbasi V., Rahimian H. and Tajick-Ghanbari M.A. 2013. Genetic variability of Iranian strains of Pseudomonas syringae pv. syringae causing bacterial canker disease of stone fruits. European Journal of Plant Pathology, 135, 225-235.
There are 1 citations in total.

Details

Journal Section Makaleler
Authors

Mustafa Mirik

Cansu Öksel This is me

Merve Özdemir This is me

Publication Date December 31, 2016
Submission Date January 18, 2017
Acceptance Date November 18, 2016
Published in Issue Year 2016 Volume: 56 Issue: 4

Cite

APA Mirik, M., Öksel, C., & Özdemir, M. (2016). Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province. Plant Protection Bulletin, 56(4), 385-397.
AMA Mirik M, Öksel C, Özdemir M. Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province. Plant Protection Bulletin. December 2016;56(4):385-397.
Chicago Mirik, Mustafa, Cansu Öksel, and Merve Özdemir. “Characterization of Causal Agents of Bacterial Canker on Sweet Cherry Trees in Tekirdağ Province”. Plant Protection Bulletin 56, no. 4 (December 2016): 385-97.
EndNote Mirik M, Öksel C, Özdemir M (December 1, 2016) Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province. Plant Protection Bulletin 56 4 385–397.
IEEE M. Mirik, C. Öksel, and M. Özdemir, “Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province”, Plant Protection Bulletin, vol. 56, no. 4, pp. 385–397, 2016.
ISNAD Mirik, Mustafa et al. “Characterization of Causal Agents of Bacterial Canker on Sweet Cherry Trees in Tekirdağ Province”. Plant Protection Bulletin 56/4 (December 2016), 385-397.
JAMA Mirik M, Öksel C, Özdemir M. Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province. Plant Protection Bulletin. 2016;56:385–397.
MLA Mirik, Mustafa et al. “Characterization of Causal Agents of Bacterial Canker on Sweet Cherry Trees in Tekirdağ Province”. Plant Protection Bulletin, vol. 56, no. 4, 2016, pp. 385-97.
Vancouver Mirik M, Öksel C, Özdemir M. Characterization of causal agents of bacterial canker on sweet cherry trees in Tekirdağ province. Plant Protection Bulletin. 2016;56(4):385-97.

136481365013649