Research Article
Prevalence and molecular characterization of Tomato spotted wilt virus in pepper fields in Tokat province
Abstract
The study was conducted in Tokat Center, Niksar, Erbaa and Pazar districts where peppers were grown in the summer of 2016, and leaf samples were collected from plants suspected of the virus. During the surveys, a total of 324 plant samples were collected and the infected pepper samples were subjected to DAS-ELISA test with (Tomato spotted wilt virus) TSWV-specific antiserum, and RT-PCR was performed with virus-specific primers. In DAS-ELISA studies, 324 plants were tested and 13% of the samples were found to be TSWV infected. Samples that were positive in ELISA test were subjected to RT-PCR with nucleocapsid gene specific primers in the S segment and three samples were sent for sequence analysis. According to results, Turkey TSWV isolates Np gene region have shown 98-99% nucleotide identity with the isolates from France and South Korea and grouped with them same group.
Supporting Institution
Tokat Gaziosmanpasa University Scientific Research Project
Project Number
2016/25
References
- Aramburu J., Marti M., 2003. The occurrence in northeast Spain of a variant of Tomato spotted wilt virus (TSWV) that breaks resistance in tomato (Lycopersicon esculentum) containing the Sw-5 gene. Plant Pathol 52: 407–407.
- Arli-Sokmen M A, Mennan H, Sevik M A, Ecevit O., 2005. Occurrence of viruses in field-grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey, Phytoparasitica 33(4): 347-358
- Astruc N., Marcos J.F., Macquaıre G., Candresse T., Pallás V., 1996. Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology 102:837-846
- Azeri T., 1981. Preliminary report of tomato spotted wilt virus and its epidemiy on tobacco in the Çanakkale region of Türkiye. J. Turkish Phytopath., 10 (2-3): 79-87.
- Bogatzevska N., Stoimenova E., Mitrev S., 2007. Bacterial and virus diseases spread in Bulgaria and Macedonia on field and greenhouse pepper. Plant Prot 18:17–21
- Boukema I.W., 1982. Resistance to a new strain of TMV in Capsicum chacoense Hunz. Capsicum News, l1:49-51
- Brittlebank C.C., 1919. Tomato diseases. Journal of Department of Agriculture of Victoria Australia 17: 231-235.
- Buzkan N., Arpacı B.B., Simon V., Fakhfakh H., Moury B., 2013. High prevalence of poleroviruses in field-grown pepper in Turkey and Tunisia. Arch Virol. 158(4): 881-885.
- Chen L.F., Vivoda E., Gilbertson R.L., 2011. Genetic diversity in curtoviruses: a highly divergent strain of Beet mild curly top virus associated with an outbreak of curly top disease in pepper in Mexico. Archive Virology, 156:547555.
- Clark M.F., Adams A.N., 1977. Characteristic of microplate method of Enzyme-Linked Immunosorbent Assay for detection of plant viruses. Journal of General Virology, 34: 475-483
- Debreczeni D. E., Rubio L., Aramburu J., Lopez C., Galipienso L., Soler, S., Belliure B., (2014): Transmission of Tomato spotted wilt virus isolates Able and Unable To Overcome Tomato or Pepper Resistance by Its Vector Frankliniella occidentalis. – Annals of Applied Biology.164(2): 182-189.
- Deligoz I., Arli Sokmen M., Sari S., 2014. First report of resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant sweetpepper cultivars in Turkey. New Disease Reports 30, 26.
- Dombrovsky A., Glanz E., Pearlsman M., Lachman O., Antignus Y., 2010. Characterization of Pepper yellow leaf curl virus, a tentative new Polerovirus species causing a yellowing disease of pepper. Phytoparasitica. Volume 38, Issue 5, 477-486
- Edwardson J.R., Christie R.G., 1986. Viruses Infecting Forage Legumes. University of Florida Agricultural Experiment Station, Gainesville, 742
- Fajinmi A.A., 2013. Pepper veinal mottle virus, a potyvirus of pepper under tropical conditions. International Journal of Vegetable, 19:150–156
- Fereres A., Perez P., Gemeno C., Ponz F., 1993. Transmission of Spanish pepper‐PVY and potato‐PVY isolates by aphid (Homoptera, Aphididae) vectors—epidemiological implications. Environ Entomol., 22:1260–1265
- Fidan H., 2016. Antalya’da Örtü Altı Domates ve Biber Alanlarında Dayanıklılık Kıran Tomato spotted wilt virus (TSWV) İzolatların Genetik Kıyaslanması, VI. Türkiye Bitki Koruma Kongresi Konya, Türkiye, s. 560.
- Gabor B., Krizbai L., Horvath J., Takacs A., 2012. Resistance breaking strain of Tomato spotted wilt virus (TSWV) on resistant pepper cultivars in Hungary. Proceedings of the International Symposium on Current Trends in Plant Protection, Belgrade, Serbia, 25-28th September, 239-241.
- Garcia-Arenal F., Escriu F., Aranda M.A., Alonso-Prados J.L., Malpica J.M., Fraile A., 2000. Molecular epidemiology of Cucumber mosaic virus and its satellite RNA. Virus Res., 71:1-8
- Genda Y., Kanda A., Hamada H., Sato K., Ohnishi J., Tsuda S., 2007. Two amino acid substitutions in the coat protein of pepper mild mottle virus are responsible for overcoming the L4 gene-mediated resistance in Capsicum spp. Phytopathology, 97:787-793
- Gilardi P., Garcia-Luque I., Serra M.T., 2004. The coat protein of Tobamovirus acts as elicitor of both L2 and L4 gene-mediated resistance in Capsicum. Journal of General Virology, 85:2077-2085
- Guldur M.E., Caglar B.K., 2006. Outbreaks of Pepper mild mottle virus in greenhouses in Sanliurfa, Turkey. Journal Plant Pathology, 88:339-342
- Hobbs H.A., Black L.L., Johnson R.R., Valverde R.A., 1994. Differences in reactions among Tomato spotted wilt virus isolates to three resistant Capsicum chinense lines. Plant Disease 78, 1220.
- Kamberoğlu M.A., Alan B., 2011. Occurrence of Tomato spotted wilt virus in lettuce in Cukurova region of Turkey. International Journal of Agriculture and Biology, 13: 431–434.
- Kamberoğlu M.A., Çalışkan F., Alan B., 2009. First report of tomato spotted wilt virus on eggplant in Turkey. Journal of Plant Pathology, Disease Note, 91 (1), 231-240.
- Karavina C., Kubba A., 2017. Detection and characterization of Tomato spotted wilt virus infecting field and greenhouse-grown crops in Zimbabwe. European Journal Plant Pathology. 149:933–944
- Lian S., Lee J. S., Cho W. K., Kim M. K., Choi H. S., Kim K. H., 2013. Phylogenetic and recombination analysis of tomato spotted wilt virus. Plos One 8: 1-11.
- Lopez C., Aramburu J., Galipienso L., Soler S., Nuez F., Rubio,L., 2011. Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of tomato spotted wilt virus. Journal of General Virology 92: 210-215.
- Maiss E., Ivanova L., Breyel E., Adam G., 1991. Cloning and sequencing of the S RNA from a Bulgarian isolate of Tomato spotted wilt virus. Journal of General Virology 72, 461-464.
- Margaria P., Ciuffo M., Turina M., 2004. Resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant pepper cultivars in Almeria Spain, Plant Pathology 53(6): 795-795
- Margaria P., Ciuffo M., Turina M., 2004. Resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant pepper cultivars in Almeria (Spain), Plant Pathology 53, 795.
- Milne R.G., Francki R.I., (1984). Should tomato spotted wilt virus be considered as a possible member of the family Bunyaviridae? Intervirology, 22, 72–76
- Nakazono-Nagaoka E., Suzuki M., Kosaka Y., Natsuaki T., 2005. RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus. Journal of General Plant Pathology., 71:243-246
- Oreshkoviky B.K., Rusevski R., Kuzmanovska B., Jankulovska M., Popovski Z., Bandzo K., 2018. Occurrence of plant viruses on pepper cultivated in open fields in R. Macedonia and partial characterization of cucumber mosaic virus isolates. Journal Plant Patholog, 100, 485–491
- Ozdag Y., Sertkaya G., 2017. Investigation on Viruses Causing Yellowing Disease in Pepper in Hatay-Turkey. Journal of Agricultural Faculty of Mustafa Kemal University. 22(1):16-22
- Palloix A., Abak K., Gognalons P., Daubeze A.M., Guldur M., Memouchi G., Gebre-Selaissie K., 1994. Virus diseases infecting pepper crops in Turkey. Proceedings of 9th Congress of the Mediterranean Phytopathological Union, Kuşadası, Aydın, 469-472
- Peiró A., Cañizares M.C., Rubio L., López C., Moriones E., Aramburu J., Sánchez-Navarro J., 2014. The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance. Molecular Plant Pathology 15(8), 802–813
- Roggero P., Masenga V., Tavella L., 2002. Field isolates of Tomato spotted wilt virus overcoming resistance in pepper and their spread to other hosts in Italy. Plant Disease 86, 950-954.
- Sharman M., Persley D.M., 2006. Field isolates of Tomato spotted wilt virus overcoming resistance in Capsicum in Australia. Australasian Plant Pathology 35, 123-128.
- Sin B., 2015. Characterization of Tomato Spotted Wilt Virus (TSWV) Isolates Causing Infection In Tomato Fields in Amasya Samsun and Tokat Provinces. Master Thesis. Ondokuz Mayıs University. Samsun
- Singh R.P., Valkonen J.P.T., Gray S.M., Boonham N., Jones R.A.C., Kerlan C., Schubert J., 2008. Discussion Paper: The naming of Potato virus Y strains infecting potato. Arch Virol., 153:1-13
- Tekinel N., Dolar M. S., Sagsoz S., Salcan Y., 1969. Mersin bölgesinde ekonomik bakımdan önemli bazı sebzelerin virüsleri üzerinde araştırmalar. Bitki Koruma Bülteri, 9: 37-49.
- Turhan P., Korkmaz S., 2006. Determination of Tomato Spotted Wilt Virus Using Serological and Biological Methods in Tomatoes Grown in Çanakkale Province. Journal of Agricultural Sciences, 12: 130-136
- Yardımcı N., Çulal-Kılıç H., 2009. Tomato spotted wilt virüs in vegetable growing areas in the West mediterranean region of Turkey. African Jorunal of Biotecnology 8 (18), s:4539-4541.
Tokat ilinde biber alanlarında Tomato spotted wilt virus’ın yaygınlığı ve moleküler karakterizasyonu
Abstract
Çalışma kapsamında 2016 yılı yaz döneminde biber yetiştirilen Tokat ili Merkez, Niksar, Erbaa ve Pazar ilçelerine sürveyler düzenlenmiş ve virüs şüphesi gösteren biber bitkilerinden yaprak örneği toplanmıştır. Sürvey çalışmaları sonucunda toplanan 324 adet bitki yaprak örneği Tomato spotted wilt virus (TSWV)’e spesifik antiserum ile DAS-ELISA testine tabii tutulduktan sonra virüse spesifik primerlerle RT-PCR işlemi gerçekleştirilmiştir. Testlenen örneklerin %13’nün TSWV ile enfekteli olduğu tespit edilmiştir. ELISA testinde pozitif çıkan örnekler S segmentinde yer alan Nucleocapsid proteine (Np) spesifik primerlerle RT-PCR işlemine tabi tutulmuş ve pozitif bant elde edilen örneklerden 3’ü sekans analizine gönderilmiştir. Analiz sonuçlarına göre Türkiye TSWV izolatlarının Np gen bölgesi Fransa ve Güney Kore izolatları ile %98-99 nükleotid benzerliği göstermiştir ve filogenetik ağaçta aynı grupta kümelenmiştir.Anahtar kelimeler: biber, RT-PCR, Tomato spotted wilt virus, Tokat
Keywords
Project Number
2016/25
References
- Aramburu J., Marti M., 2003. The occurrence in northeast Spain of a variant of Tomato spotted wilt virus (TSWV) that breaks resistance in tomato (Lycopersicon esculentum) containing the Sw-5 gene. Plant Pathol 52: 407–407.
- Arli-Sokmen M A, Mennan H, Sevik M A, Ecevit O., 2005. Occurrence of viruses in field-grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey, Phytoparasitica 33(4): 347-358
- Astruc N., Marcos J.F., Macquaıre G., Candresse T., Pallás V., 1996. Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology 102:837-846
- Azeri T., 1981. Preliminary report of tomato spotted wilt virus and its epidemiy on tobacco in the Çanakkale region of Türkiye. J. Turkish Phytopath., 10 (2-3): 79-87.
- Bogatzevska N., Stoimenova E., Mitrev S., 2007. Bacterial and virus diseases spread in Bulgaria and Macedonia on field and greenhouse pepper. Plant Prot 18:17–21
- Boukema I.W., 1982. Resistance to a new strain of TMV in Capsicum chacoense Hunz. Capsicum News, l1:49-51
- Brittlebank C.C., 1919. Tomato diseases. Journal of Department of Agriculture of Victoria Australia 17: 231-235.
- Buzkan N., Arpacı B.B., Simon V., Fakhfakh H., Moury B., 2013. High prevalence of poleroviruses in field-grown pepper in Turkey and Tunisia. Arch Virol. 158(4): 881-885.
- Chen L.F., Vivoda E., Gilbertson R.L., 2011. Genetic diversity in curtoviruses: a highly divergent strain of Beet mild curly top virus associated with an outbreak of curly top disease in pepper in Mexico. Archive Virology, 156:547555.
- Clark M.F., Adams A.N., 1977. Characteristic of microplate method of Enzyme-Linked Immunosorbent Assay for detection of plant viruses. Journal of General Virology, 34: 475-483
- Debreczeni D. E., Rubio L., Aramburu J., Lopez C., Galipienso L., Soler, S., Belliure B., (2014): Transmission of Tomato spotted wilt virus isolates Able and Unable To Overcome Tomato or Pepper Resistance by Its Vector Frankliniella occidentalis. – Annals of Applied Biology.164(2): 182-189.
- Deligoz I., Arli Sokmen M., Sari S., 2014. First report of resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant sweetpepper cultivars in Turkey. New Disease Reports 30, 26.
- Dombrovsky A., Glanz E., Pearlsman M., Lachman O., Antignus Y., 2010. Characterization of Pepper yellow leaf curl virus, a tentative new Polerovirus species causing a yellowing disease of pepper. Phytoparasitica. Volume 38, Issue 5, 477-486
- Edwardson J.R., Christie R.G., 1986. Viruses Infecting Forage Legumes. University of Florida Agricultural Experiment Station, Gainesville, 742
- Fajinmi A.A., 2013. Pepper veinal mottle virus, a potyvirus of pepper under tropical conditions. International Journal of Vegetable, 19:150–156
- Fereres A., Perez P., Gemeno C., Ponz F., 1993. Transmission of Spanish pepper‐PVY and potato‐PVY isolates by aphid (Homoptera, Aphididae) vectors—epidemiological implications. Environ Entomol., 22:1260–1265
- Fidan H., 2016. Antalya’da Örtü Altı Domates ve Biber Alanlarında Dayanıklılık Kıran Tomato spotted wilt virus (TSWV) İzolatların Genetik Kıyaslanması, VI. Türkiye Bitki Koruma Kongresi Konya, Türkiye, s. 560.
- Gabor B., Krizbai L., Horvath J., Takacs A., 2012. Resistance breaking strain of Tomato spotted wilt virus (TSWV) on resistant pepper cultivars in Hungary. Proceedings of the International Symposium on Current Trends in Plant Protection, Belgrade, Serbia, 25-28th September, 239-241.
- Garcia-Arenal F., Escriu F., Aranda M.A., Alonso-Prados J.L., Malpica J.M., Fraile A., 2000. Molecular epidemiology of Cucumber mosaic virus and its satellite RNA. Virus Res., 71:1-8
- Genda Y., Kanda A., Hamada H., Sato K., Ohnishi J., Tsuda S., 2007. Two amino acid substitutions in the coat protein of pepper mild mottle virus are responsible for overcoming the L4 gene-mediated resistance in Capsicum spp. Phytopathology, 97:787-793
- Gilardi P., Garcia-Luque I., Serra M.T., 2004. The coat protein of Tobamovirus acts as elicitor of both L2 and L4 gene-mediated resistance in Capsicum. Journal of General Virology, 85:2077-2085
- Guldur M.E., Caglar B.K., 2006. Outbreaks of Pepper mild mottle virus in greenhouses in Sanliurfa, Turkey. Journal Plant Pathology, 88:339-342
- Hobbs H.A., Black L.L., Johnson R.R., Valverde R.A., 1994. Differences in reactions among Tomato spotted wilt virus isolates to three resistant Capsicum chinense lines. Plant Disease 78, 1220.
- Kamberoğlu M.A., Alan B., 2011. Occurrence of Tomato spotted wilt virus in lettuce in Cukurova region of Turkey. International Journal of Agriculture and Biology, 13: 431–434.
- Kamberoğlu M.A., Çalışkan F., Alan B., 2009. First report of tomato spotted wilt virus on eggplant in Turkey. Journal of Plant Pathology, Disease Note, 91 (1), 231-240.
- Karavina C., Kubba A., 2017. Detection and characterization of Tomato spotted wilt virus infecting field and greenhouse-grown crops in Zimbabwe. European Journal Plant Pathology. 149:933–944
- Lian S., Lee J. S., Cho W. K., Kim M. K., Choi H. S., Kim K. H., 2013. Phylogenetic and recombination analysis of tomato spotted wilt virus. Plos One 8: 1-11.
- Lopez C., Aramburu J., Galipienso L., Soler S., Nuez F., Rubio,L., 2011. Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of tomato spotted wilt virus. Journal of General Virology 92: 210-215.
- Maiss E., Ivanova L., Breyel E., Adam G., 1991. Cloning and sequencing of the S RNA from a Bulgarian isolate of Tomato spotted wilt virus. Journal of General Virology 72, 461-464.
- Margaria P., Ciuffo M., Turina M., 2004. Resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant pepper cultivars in Almeria Spain, Plant Pathology 53(6): 795-795
- Margaria P., Ciuffo M., Turina M., 2004. Resistance breaking strain of Tomato spotted wilt virus (Tospovirus; Bunyaviridae) on resistant pepper cultivars in Almeria (Spain), Plant Pathology 53, 795.
- Milne R.G., Francki R.I., (1984). Should tomato spotted wilt virus be considered as a possible member of the family Bunyaviridae? Intervirology, 22, 72–76
- Nakazono-Nagaoka E., Suzuki M., Kosaka Y., Natsuaki T., 2005. RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus. Journal of General Plant Pathology., 71:243-246
- Oreshkoviky B.K., Rusevski R., Kuzmanovska B., Jankulovska M., Popovski Z., Bandzo K., 2018. Occurrence of plant viruses on pepper cultivated in open fields in R. Macedonia and partial characterization of cucumber mosaic virus isolates. Journal Plant Patholog, 100, 485–491
- Ozdag Y., Sertkaya G., 2017. Investigation on Viruses Causing Yellowing Disease in Pepper in Hatay-Turkey. Journal of Agricultural Faculty of Mustafa Kemal University. 22(1):16-22
- Palloix A., Abak K., Gognalons P., Daubeze A.M., Guldur M., Memouchi G., Gebre-Selaissie K., 1994. Virus diseases infecting pepper crops in Turkey. Proceedings of 9th Congress of the Mediterranean Phytopathological Union, Kuşadası, Aydın, 469-472
- Peiró A., Cañizares M.C., Rubio L., López C., Moriones E., Aramburu J., Sánchez-Navarro J., 2014. The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance. Molecular Plant Pathology 15(8), 802–813
- Roggero P., Masenga V., Tavella L., 2002. Field isolates of Tomato spotted wilt virus overcoming resistance in pepper and their spread to other hosts in Italy. Plant Disease 86, 950-954.
- Sharman M., Persley D.M., 2006. Field isolates of Tomato spotted wilt virus overcoming resistance in Capsicum in Australia. Australasian Plant Pathology 35, 123-128.
- Sin B., 2015. Characterization of Tomato Spotted Wilt Virus (TSWV) Isolates Causing Infection In Tomato Fields in Amasya Samsun and Tokat Provinces. Master Thesis. Ondokuz Mayıs University. Samsun
- Singh R.P., Valkonen J.P.T., Gray S.M., Boonham N., Jones R.A.C., Kerlan C., Schubert J., 2008. Discussion Paper: The naming of Potato virus Y strains infecting potato. Arch Virol., 153:1-13
- Tekinel N., Dolar M. S., Sagsoz S., Salcan Y., 1969. Mersin bölgesinde ekonomik bakımdan önemli bazı sebzelerin virüsleri üzerinde araştırmalar. Bitki Koruma Bülteri, 9: 37-49.
- Turhan P., Korkmaz S., 2006. Determination of Tomato Spotted Wilt Virus Using Serological and Biological Methods in Tomatoes Grown in Çanakkale Province. Journal of Agricultural Sciences, 12: 130-136
- Yardımcı N., Çulal-Kılıç H., 2009. Tomato spotted wilt virüs in vegetable growing areas in the West mediterranean region of Turkey. African Jorunal of Biotecnology 8 (18), s:4539-4541.
There are 44 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Makaleler |
| Authors | |
| Project Number | 2016/25 |
| Publication Date | September 30, 2021 |
| Submission Date | March 17, 2021 |
| Acceptance Date | June 3, 2021 |
| Published in Issue | Year 2021 Volume: 61 Issue: 3 |
