Research Article
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Year 2022, Volume: 12 Issue: 2, 390 - 395, 30.06.2022
https://doi.org/10.33808/clinexphealthsci.915721

Abstract

Supporting Institution

Gaziantep Üniversitesi

References

  • [1] Rautava S. Early microbial contact, the breast milk microbiome and child health. J Dev Orig Health Dis 2016;7(1):5-14.
  • [2] Aslan NN. Yardımcı H. Anne Sütü ve Mikrobiyota. Turk Klin J Nutr Diet-Special Topics 2017;3(2):95-100. (Turkish)
  • [3] Arrieta MC, Stiemsma LT, Amenyogbe N, Brown EM, Finlay B. The intestinal microbiome in early life: health and disease. Front Immunol 2014;5:427.
  • [4] Jeurink PV, Van Bergenhenegouwen J, Jimenez E, Knippels LM, Fernández L, Garssen J. Human milk: a source of more life than we imagine. Benef Microbes 2012;4(1):17-30.
  • [5] Sherman PM, Johnson-Henry KC, Yeong HP, Ngo PSC, Goulet J, Tompkins TA. Probiotics reduce enterohemorrhagic Escherichia coli O157:H7 – and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements. Infect Immun 2005;73:5183-5188.
  • [6] Fernández L, Delgado S, Herrero H, Maldonado A, Rodríguez JM. The bacteriocin nisin, an effective agent for the treatment of staphylococcal mastitis during lactation. J Hum Lact 2008;24(3):311-316.
  • [7] Lim YM, Barnes MB, Gras SL, McSweeney C, Lockett T, Augustin MA. Esterification of high amylose starch with short chain fatty acids modulates degradation by Bifidobacterium spp. J Funct Foods 2014;6:137-146.
  • [8] Bergmann H, Rodríguez JM, Salminen S, Szajewska H. Probiotics in human milk and probiotic supplementation in infant nutrition: a workshop report. Br J Nutr 2014;112(7),1119- 1128.
  • [9] Jacobi SK, Odle J. Nutritional factors influencing intestinal health of the neonate. Adv Nutr 2012;3:687-696.
  • [10] Martín R, Langa S, Reviriego C, Jimínez E, Marín ML, Xaus J. Human milk is a source of lactic acid bacteria for the infant gut. J Pediatr 2003;143(6):754-8.
  • [11] Urbaniak C, Burton JP, Reid G. Breast, milk and microbes: A complex relationship that does not end with lactation. Womens Health (Lond.) 2012;8(4):385-398.
  • [12] Holzapfel WH, Haberer P, Snel J, Schillinger U, Huis in’t Veld JH. Overview of gut flora and probiotics. Int J Food Microbiol 1998;41(2):85-101.
  • [13] Osmanagaoglu O, Kiran F, Ataoglu H. Evaluation of in vitro Probiotic Potential of Pediococcus pentosaceus OZF Isolated from Human Breast Milk. Probiotics Antimicrob Proteins 2010;2(3):162-174.
  • [14] Castellote C, Casillas R, Ramírez-Santana C, Pérez-Cano FJ, Castell M, Moretones MG. Premature delivery influences the immunological composition of colostrum and transitional and mature human milk. J Nutr 2011;141(6):1181-1187.
  • [15] Kwon HS, Yang EH, Yeon SW, Kang BH, Kim TY. Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA. FEMS Microbiol Lett 2004;239(2):267-275.
  • [16] Gomez-Gallego C, Garcia-Mantrana I, Salminen S, Collado MC. The human milk microbiome and factors influencing its composition and activity. Semin Fetal Neonatal Med 2016;21(6):400-405.
  • [17] Fernández L, Langa S, Martín V, Maldonado A, Jiménez E, Martín R. The human milk microbiota: origin and potential roles in health and disease. Pharmacol Res 2013;69(1):1-10.
  • [18] Soto A, Martín V, Jiménez E, Mader I, Rodríguez JM, Fernández L. Lactobacilli and bifidobacteria in human breast milk: influence of antibiotherapy and other host and clinical factors. J Pediatr Gastroenterol Nutr 2014;59(1):78-88.
  • [19] Bode L, McGuire M, Rodriguez JM, Geddes DT, Hassiotou F, Hartmann PE. It’s alive: microbes and cells in human milk and their potential benefits to mother and infant. Adv Nutr 2014;5(5):571-573.
  • [20] Murphy K, Curley D, O’Callaghan TF, O’Shea CA, Dempsey EM, O’Toole PW. The Composition of human milk and infant faecal microbiota over the first three months of life: A pilot study. Sci Rep 2017;7:40597
  • [21] Boix-Amorós A, Collado MC, Mira A. Relationship between milk microbiota, bacterial load, macronutrients, and human cells during lactation. Front Microbiol 2016;7:492.
  • [22] Khodayar-Pardo P, Mira-Pascual L, Collado MC, Martínez-Costa C. Impact of lactation stage, gestational age and mode of delivery on breast milk microbiota. J Perinatol 2014;34(8):599- 605.
  • [23] Latuga MS, Stuebe A, Seed PC. A review of the source and function of microbiota in breast milk. Semin Reprod Med 2014;32(1):68-73.
  • [24] Isolauri E. Development of healthy gut microbiota early in life. J Paediatr Child Health 2012;48 Suppl 3:1-6.
  • [25] Martín V, Maldonado-Barragán A, Moles L, Rodriguez-Baños M, Campo RD, Fernández L. Sharing of bacterial strains between breast milk and infant feces. J Hum Lact 2012;28(1):36-44.
  • [26] Schanche M, Avershina E, Dotterud C, Øien T, Storrø O, Johnsen R. High-resolution analyses of overlap in the microbiota between mothers and their children. Curr Microbiol 2015;71(2):283-290.
  • [27] Martin R, Heilig GH, Zoetendal EG, Smidt H, Rodríguez JM. Diversity of the Lactobacillus group in breast milk and vagina of healthy women and potential role in the colonization of the infant gut. J Appl Microbiol 2007;103(6):2638-2644.
  • [28] Goldsmith F, O’Sullivan A, Smilowitz JT, Freeman SL. Lactation and Intestinal Microbiota: How Early Diet Shapes the Infant Gut. J Mammary Gland Biol Neoplasia 2015;20(3-4):149-158.
  • [29] Mueller NT, Bakacs E, Combellick J, Grigoryan Z, Dominguez Bello MG. The infant microbiome development: mom matters. Trends Mol Med 2015;21(2):109-117.
  • [30] Guaraldi F, Salvatori G. Effect of breast and formula feeding on gut microbiota shaping in newborns. Front Cell Infect Microbiol 2012;2:94.
  • [31] Coppa GV, Bruni S, Morelli L, Soldi S, Gabrielli O. The first prebiotics in humans: human milk oligosaccharides. J Clin Gastroenterol 2004;38(6 Suppl):S80-S83.
  • [32] Cabrera-Rubio R, Collado MC, Laitinen K, Salminen S, Isolauri E, Mira A. The human milk microbiome changes over lactation and is shaped by maternal weight and mode of delivery. Am J Clin Nutr 2012;96(3):544-551.
  • [33] Collado MC, Laitinen K, Salminen S, Isolauri E. Maternal weight and excessive weight gain during pregnancy modify the immunomodulatory potential of breast milk. Pediatr Res 2012;72(1):77-85.
  • [34] Laitinen K, Collado MC, Isolauri E. Early nutritional environment: focus on health effects of microbiota and probiotics. Benef Microbes 2010;1(4):383-90.
  • [35] Bai DL, Wu KM, Tarrant M. Association between intrapartum interventions and breastfeeding duration. J Midwifery Womens Health 2013;58(1):25-32.
  • [36] Neu J, Rushing J. Cesarean versus vaginal delivery: long-term infant outcomes and the hygiene hypothesis. Clin Perinatol 2011;38(2):321-331.
  • [37] Urbaniak C, Angelini M, Gloor GB, Reid G. Human milk microbiota profiles in relation to birthing method, gestation and infant gender. Microbiome 2016; 4:1

Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?

Year 2022, Volume: 12 Issue: 2, 390 - 395, 30.06.2022
https://doi.org/10.33808/clinexphealthsci.915721

Abstract

Objective; Breast milk has an important function in the formation of the intestinal flora. Cesarean section bypasses the vertical transition of vaginal flora to the baby also usually causes the late start of lactation. The difference is in birth style and lactation period may affect the microbiota of breast milk. In this study, it was aimed to investigate how Lactobacillus species found in breast milk differ by the birth style and stages of milk.
Methods; Milk samples were taken from 72 mothers who had a vaginal birth (n:36) and cesarean (n:36) were divided into three groups as colostrum (n:12), early milk (n:12), and mature milk (n:12). Lactobacillus species were investigated from milk samples by real-time PCR.
Results: While Lactobacillus was detected in 70 (97.2%) of the samples, it was not detected in 2 (2.8%) of the samples taken from women with cesarean delivery. L. acidophilus and L. rhamnosus were detected simultaneously in all transitional milk samples of women who had a vaginal birth, and 82 Lactobacillus species were detected. The species identified were 33(39.3%) L. rhamnosus, 25(29.8%) Lactobacillus spp., and 24(28.6%) L. acidophilus. The rate of detection of L. acidophilus in milk samples taken from women who gave birth was found to be significantly higher than that found in milk samples taken from women who gave birth by cesarean section (p <0.05).
Conlusion: Breast milk is not only a nutritional source but an important source of probiotics. Lactobacilli were found to be concentrated in breast milk. Also, Lactobacillus species detected in breast milk may differ according to the mode of delivery.

References

  • [1] Rautava S. Early microbial contact, the breast milk microbiome and child health. J Dev Orig Health Dis 2016;7(1):5-14.
  • [2] Aslan NN. Yardımcı H. Anne Sütü ve Mikrobiyota. Turk Klin J Nutr Diet-Special Topics 2017;3(2):95-100. (Turkish)
  • [3] Arrieta MC, Stiemsma LT, Amenyogbe N, Brown EM, Finlay B. The intestinal microbiome in early life: health and disease. Front Immunol 2014;5:427.
  • [4] Jeurink PV, Van Bergenhenegouwen J, Jimenez E, Knippels LM, Fernández L, Garssen J. Human milk: a source of more life than we imagine. Benef Microbes 2012;4(1):17-30.
  • [5] Sherman PM, Johnson-Henry KC, Yeong HP, Ngo PSC, Goulet J, Tompkins TA. Probiotics reduce enterohemorrhagic Escherichia coli O157:H7 – and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements. Infect Immun 2005;73:5183-5188.
  • [6] Fernández L, Delgado S, Herrero H, Maldonado A, Rodríguez JM. The bacteriocin nisin, an effective agent for the treatment of staphylococcal mastitis during lactation. J Hum Lact 2008;24(3):311-316.
  • [7] Lim YM, Barnes MB, Gras SL, McSweeney C, Lockett T, Augustin MA. Esterification of high amylose starch with short chain fatty acids modulates degradation by Bifidobacterium spp. J Funct Foods 2014;6:137-146.
  • [8] Bergmann H, Rodríguez JM, Salminen S, Szajewska H. Probiotics in human milk and probiotic supplementation in infant nutrition: a workshop report. Br J Nutr 2014;112(7),1119- 1128.
  • [9] Jacobi SK, Odle J. Nutritional factors influencing intestinal health of the neonate. Adv Nutr 2012;3:687-696.
  • [10] Martín R, Langa S, Reviriego C, Jimínez E, Marín ML, Xaus J. Human milk is a source of lactic acid bacteria for the infant gut. J Pediatr 2003;143(6):754-8.
  • [11] Urbaniak C, Burton JP, Reid G. Breast, milk and microbes: A complex relationship that does not end with lactation. Womens Health (Lond.) 2012;8(4):385-398.
  • [12] Holzapfel WH, Haberer P, Snel J, Schillinger U, Huis in’t Veld JH. Overview of gut flora and probiotics. Int J Food Microbiol 1998;41(2):85-101.
  • [13] Osmanagaoglu O, Kiran F, Ataoglu H. Evaluation of in vitro Probiotic Potential of Pediococcus pentosaceus OZF Isolated from Human Breast Milk. Probiotics Antimicrob Proteins 2010;2(3):162-174.
  • [14] Castellote C, Casillas R, Ramírez-Santana C, Pérez-Cano FJ, Castell M, Moretones MG. Premature delivery influences the immunological composition of colostrum and transitional and mature human milk. J Nutr 2011;141(6):1181-1187.
  • [15] Kwon HS, Yang EH, Yeon SW, Kang BH, Kim TY. Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA. FEMS Microbiol Lett 2004;239(2):267-275.
  • [16] Gomez-Gallego C, Garcia-Mantrana I, Salminen S, Collado MC. The human milk microbiome and factors influencing its composition and activity. Semin Fetal Neonatal Med 2016;21(6):400-405.
  • [17] Fernández L, Langa S, Martín V, Maldonado A, Jiménez E, Martín R. The human milk microbiota: origin and potential roles in health and disease. Pharmacol Res 2013;69(1):1-10.
  • [18] Soto A, Martín V, Jiménez E, Mader I, Rodríguez JM, Fernández L. Lactobacilli and bifidobacteria in human breast milk: influence of antibiotherapy and other host and clinical factors. J Pediatr Gastroenterol Nutr 2014;59(1):78-88.
  • [19] Bode L, McGuire M, Rodriguez JM, Geddes DT, Hassiotou F, Hartmann PE. It’s alive: microbes and cells in human milk and their potential benefits to mother and infant. Adv Nutr 2014;5(5):571-573.
  • [20] Murphy K, Curley D, O’Callaghan TF, O’Shea CA, Dempsey EM, O’Toole PW. The Composition of human milk and infant faecal microbiota over the first three months of life: A pilot study. Sci Rep 2017;7:40597
  • [21] Boix-Amorós A, Collado MC, Mira A. Relationship between milk microbiota, bacterial load, macronutrients, and human cells during lactation. Front Microbiol 2016;7:492.
  • [22] Khodayar-Pardo P, Mira-Pascual L, Collado MC, Martínez-Costa C. Impact of lactation stage, gestational age and mode of delivery on breast milk microbiota. J Perinatol 2014;34(8):599- 605.
  • [23] Latuga MS, Stuebe A, Seed PC. A review of the source and function of microbiota in breast milk. Semin Reprod Med 2014;32(1):68-73.
  • [24] Isolauri E. Development of healthy gut microbiota early in life. J Paediatr Child Health 2012;48 Suppl 3:1-6.
  • [25] Martín V, Maldonado-Barragán A, Moles L, Rodriguez-Baños M, Campo RD, Fernández L. Sharing of bacterial strains between breast milk and infant feces. J Hum Lact 2012;28(1):36-44.
  • [26] Schanche M, Avershina E, Dotterud C, Øien T, Storrø O, Johnsen R. High-resolution analyses of overlap in the microbiota between mothers and their children. Curr Microbiol 2015;71(2):283-290.
  • [27] Martin R, Heilig GH, Zoetendal EG, Smidt H, Rodríguez JM. Diversity of the Lactobacillus group in breast milk and vagina of healthy women and potential role in the colonization of the infant gut. J Appl Microbiol 2007;103(6):2638-2644.
  • [28] Goldsmith F, O’Sullivan A, Smilowitz JT, Freeman SL. Lactation and Intestinal Microbiota: How Early Diet Shapes the Infant Gut. J Mammary Gland Biol Neoplasia 2015;20(3-4):149-158.
  • [29] Mueller NT, Bakacs E, Combellick J, Grigoryan Z, Dominguez Bello MG. The infant microbiome development: mom matters. Trends Mol Med 2015;21(2):109-117.
  • [30] Guaraldi F, Salvatori G. Effect of breast and formula feeding on gut microbiota shaping in newborns. Front Cell Infect Microbiol 2012;2:94.
  • [31] Coppa GV, Bruni S, Morelli L, Soldi S, Gabrielli O. The first prebiotics in humans: human milk oligosaccharides. J Clin Gastroenterol 2004;38(6 Suppl):S80-S83.
  • [32] Cabrera-Rubio R, Collado MC, Laitinen K, Salminen S, Isolauri E, Mira A. The human milk microbiome changes over lactation and is shaped by maternal weight and mode of delivery. Am J Clin Nutr 2012;96(3):544-551.
  • [33] Collado MC, Laitinen K, Salminen S, Isolauri E. Maternal weight and excessive weight gain during pregnancy modify the immunomodulatory potential of breast milk. Pediatr Res 2012;72(1):77-85.
  • [34] Laitinen K, Collado MC, Isolauri E. Early nutritional environment: focus on health effects of microbiota and probiotics. Benef Microbes 2010;1(4):383-90.
  • [35] Bai DL, Wu KM, Tarrant M. Association between intrapartum interventions and breastfeeding duration. J Midwifery Womens Health 2013;58(1):25-32.
  • [36] Neu J, Rushing J. Cesarean versus vaginal delivery: long-term infant outcomes and the hygiene hypothesis. Clin Perinatol 2011;38(2):321-331.
  • [37] Urbaniak C, Angelini M, Gloor GB, Reid G. Human milk microbiota profiles in relation to birthing method, gestation and infant gender. Microbiome 2016; 4:1
There are 37 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Aya Daif This is me 0000-0001-5550-9785

Yasemin Zer 0000-0002-9078-9900

Mehmet Erinmez 0000-0002-3570-3510

Publication Date June 30, 2022
Submission Date April 14, 2021
Published in Issue Year 2022 Volume: 12 Issue: 2

Cite

APA Daif, A., Zer, Y., & Erinmez, M. (2022). Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?. Clinical and Experimental Health Sciences, 12(2), 390-395. https://doi.org/10.33808/clinexphealthsci.915721
AMA Daif A, Zer Y, Erinmez M. Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?. Clinical and Experimental Health Sciences. June 2022;12(2):390-395. doi:10.33808/clinexphealthsci.915721
Chicago Daif, Aya, Yasemin Zer, and Mehmet Erinmez. “Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?”. Clinical and Experimental Health Sciences 12, no. 2 (June 2022): 390-95. https://doi.org/10.33808/clinexphealthsci.915721.
EndNote Daif A, Zer Y, Erinmez M (June 1, 2022) Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?. Clinical and Experimental Health Sciences 12 2 390–395.
IEEE A. Daif, Y. Zer, and M. Erinmez, “Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?”, Clinical and Experimental Health Sciences, vol. 12, no. 2, pp. 390–395, 2022, doi: 10.33808/clinexphealthsci.915721.
ISNAD Daif, Aya et al. “Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?”. Clinical and Experimental Health Sciences 12/2 (June 2022), 390-395. https://doi.org/10.33808/clinexphealthsci.915721.
JAMA Daif A, Zer Y, Erinmez M. Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?. Clinical and Experimental Health Sciences. 2022;12:390–395.
MLA Daif, Aya et al. “Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?”. Clinical and Experimental Health Sciences, vol. 12, no. 2, 2022, pp. 390-5, doi:10.33808/clinexphealthsci.915721.
Vancouver Daif A, Zer Y, Erinmez M. Lactobacillus Species in Breast Milk: Do They Get Affected by Birth Style?. Clinical and Experimental Health Sciences. 2022;12(2):390-5.

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