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The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice

Year 2014, Volume: 35 Issue: 1, 1 - 11, 19.03.2014

Abstract

References

  • Zhang, L., Eastmond, D.A., Smith, M.T., 2002. The nature of chromosomal aberrations detected in humans exposed to benzene. Crit. Rev. Toxicol. 32, 1–42.
  • Holeckova, B., Piesova, E., Sivikova, K., Dianovsky, J., 2004. Chromosomal aberrations in humans induced by benzene. Ann. Agric. Environ. 11, 175–179.
  • Dere, E., Gyborova, S., Aydın, H., 2003. The effect of benzene on serum hormones and the activity of some enzymes in different tissues of rats. Acta Veterinaria. 53, 87-101.
  • Abousalem, M., Elgerwi, A., El-Mashad, A., 2012. Biotoxic and haemotoxic effects of air pollutants at a benzene station on albino rat. Int. J. Anim. Ve.t Adv. 4, 187-190.
  • Turhan, A., Dere, E., 2007. The Effect of benzene on the activity of adenosine deaminase in tissues of rats. J Biochem. Mol. Biol. 40, 295-301.
  • Farris, G.M., Robinson, S.N., Gaido, K.W., Wong, B.A., Wong, V.A., Hahn, W.P., Shah, R.S., 1997. Benzene-induced hematotoxicity and bone marrow compensation in B6C3F1 mice. Fund. Appl. Toxicol. 36, 119-129.
  • Forni, A., 1996. Benzene-induced chromosome aberrations: A follow-up study. Environ. Health Perspect. 104, 1309-1312.
  • Oshiro, Y., Balwierz, P.S., Witz, G., 2001. Micronucleus formation in mouse bone marrow cells in vivo in response to trans, trans-muconaldehyde. Toxicol Lett. 121, 159–166.
  • Ibrahim, K.S., Saleh, Z.A., Farrag, A.R., Shaban, E.E., 2011. Protective effects of zinc and selenium against benzene toxicity in rats. Toxicol. Ind. Health. 27, 537-545.
  • Saha, S., Mukhopadhyay, M.K., Ghosh, P.D., Nath, D., Effect of methanolic leaf extract of Ocimum basilicum L. on benzene-induced hematotoxicity in mice. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine 2012; Article ID 176385, doi:10.1155/2012/176385.
  • Abd El Kader, M.A., El-Sammad, N.M., Fyiad, A.A., 2011. Effect of grape seeds extract in the modulation of matrix metalloproteinase-9 activity and oxidative stress induced by doxorubicin in mice. Life Sci. J. 8, 510-515.
  • Ahmed, A.A.E., Fatani, A.J., 2007. Protective effect of grape seeds proanthocyanidins against naphthalene-induced hepatotoxicity in rats. Saudi Pharm. J. 15, 38–47.
  • Raina, K., Singh, R.P., Agarwal, R., Agarwal, C., 2007. Oral grape seed extract inhibits prostate tumor growth and progression in TRAMP mice. Cancer Res. 67, 5976–5982.
  • Ariga, T., 2004. The antioxidative function, preventive action on disease and utilization of proanthocyanidins. Biofactors. 21, 197–201.
  • Bagchi, D., Bagchi, M., Stohs, S.J., Das, D.K., Ray, SD., Kuszynski, C.A., Joshi, S.S., 2000. Pruess HG: Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology. 148, 187–197.
  • Angelosanto, F.A., Blackburn, G.R., Schreiner, C.A., Mackerer, C.R., 1996. Benzene induces a dose-responsive increase in the frequency of micronucleated cells in rat Zymbal glands. Environ. Health Pers. 104, 1331-1336.
  • Yalçın, E., Oruç, E., Çavuşoğlu, K., Yapar, K., 2010. Protective role of grape seed extract against doxorubicin-induced cardiotoxicity and genotoxicity in albino mice. J. Med. Food. 13, 917-9
  • Te-Hsiu, M.A., Zhou, X., Loarco, G.F., Arreola, G.G., Lecona, S.U., 1995. Mouseerythrocyte micronucleus (MUS-EMN) assay on the clastogenicity of industrial wastewater. Rev. Int. Contam. Ambient. 11, 95–98.
  • Titenko-Holland, N., Windham, G., Kolachana, P., Reinisch, F., Parvatham, S., Osorio, A.M., Smith, M.T., 1997. Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mutat. Res. 388, 85–95.
  • Ozkul, Y., Donmez, H., Erenmemisoglu, A., Demirtas, H., Imamoglu, N., 1997. Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis. 12, 285–287.
  • Fenech, M., Chang, W.P., Kirsch-Volders, M., Holland, N., Bonassi, S., Zeiger, E., 2003. Human micronucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 534, 65–75.
  • Beyersmann, D., Hechtenberg, S., 1997. Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicol. Appl. Pharmacol. 144, 247–261.
  • Savage, J.R., 1976. Classification and relationships of induced chromosomal structural changes. J. Med. Genet. 13, 103–122.
  • Morgan, A.M., El-Tawil, O.S., 2006. Extract againist acrylonitrile-induced mutagenicity in rats. potential protective effect of grape seed. J. Egypt Soc. Toxicol. 34, 23-31.
  • Ahmed, A.A.E., Fatani, A.J., 2007. Protective effect of grape seeds proanthocyanidins against naphthalene-induced hepatotoxicity in rats. Saudi Pharm. J. 15, 38–47.
  • Zhao, Z., Wang, J., Chen, Y., Agarwal, R., 1999. Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiationpromotion protocol and identification of procyanidin B5-3-gallate as the most effective antioxidant constituent. Carcinogenesis. 20, 1737–1745.
  • Arii, M., Miki, M., Hosoyama, R., Ariga, H., Yamaji, N., Kataoka, S., 1998. Chemopreventive effect of grape seed extract on intestinal carcinogenesis in the APCMin mouse. Proc. Am. Assoc. Cancer Res. 39,20.
  • Raina, K., Singh, R.P., Agarwal, R., Agarwa,l C., 2007. Oral grape seed extract inhibits prostate tumor growth and progression in TRAMP mice. Cancer Res. 67, 5976–5982.
  • Hassan, N.S., Raafat, B.M., Aziz, S.W., 2010. Modulator role of grape seed extract on erythrocyte hemolysis and oxidative stress induced by microwave irradiation in rats. New York Sci J. 3, 66-73.

The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice

Year 2014, Volume: 35 Issue: 1, 1 - 11, 19.03.2014

Abstract

Benzene is an aromatic hydrocarbon often used for industrial purposes. The objective of the present study was to investigate the possible in vivo protective effects of grape seed extract (GSE) against benzene-induced toxicity in Swiss albino mice. 36 mice were divided into six groups of six animals of each, having free access to food and water ad libitum. The control group received distilled water alone, whereas mice in the treatment groups received GSE alone (50 or 150 mg/kg of body weight), benzene alone (250 mg/kg of body weight), or GSE + benzene for 50 weeks. All mice (100%) survived until the end of experiment and were sacrificed at the end of 24 hours. Bone marrow tissue was analyzed for micronucleus (MN) frequency, chromosomal aberrations (CAs), and pathological damages. Benzene alone–treated mice presented higher frequencies (P<.05) of MNs, CAs, and abnormal metaphases compared with the controls; moreover, the mitotic index was lower than in controls (P<.05). Oral treatment with GSE significantly ameliorated the indices of hepatotoxicity, nephrotoxicity, lipid peroxidation, and genotoxicity induced by benzene. Both doses of GSE tested provided significant protection against benzene induced toxicity, and its strongest effect was observed at the dose level of 150 mg/kg of body weight. Consequently, it was found that GSE has a significant protective effect against benzene-induced toxicity, and its protective effect is dose dependent.

References

  • Zhang, L., Eastmond, D.A., Smith, M.T., 2002. The nature of chromosomal aberrations detected in humans exposed to benzene. Crit. Rev. Toxicol. 32, 1–42.
  • Holeckova, B., Piesova, E., Sivikova, K., Dianovsky, J., 2004. Chromosomal aberrations in humans induced by benzene. Ann. Agric. Environ. 11, 175–179.
  • Dere, E., Gyborova, S., Aydın, H., 2003. The effect of benzene on serum hormones and the activity of some enzymes in different tissues of rats. Acta Veterinaria. 53, 87-101.
  • Abousalem, M., Elgerwi, A., El-Mashad, A., 2012. Biotoxic and haemotoxic effects of air pollutants at a benzene station on albino rat. Int. J. Anim. Ve.t Adv. 4, 187-190.
  • Turhan, A., Dere, E., 2007. The Effect of benzene on the activity of adenosine deaminase in tissues of rats. J Biochem. Mol. Biol. 40, 295-301.
  • Farris, G.M., Robinson, S.N., Gaido, K.W., Wong, B.A., Wong, V.A., Hahn, W.P., Shah, R.S., 1997. Benzene-induced hematotoxicity and bone marrow compensation in B6C3F1 mice. Fund. Appl. Toxicol. 36, 119-129.
  • Forni, A., 1996. Benzene-induced chromosome aberrations: A follow-up study. Environ. Health Perspect. 104, 1309-1312.
  • Oshiro, Y., Balwierz, P.S., Witz, G., 2001. Micronucleus formation in mouse bone marrow cells in vivo in response to trans, trans-muconaldehyde. Toxicol Lett. 121, 159–166.
  • Ibrahim, K.S., Saleh, Z.A., Farrag, A.R., Shaban, E.E., 2011. Protective effects of zinc and selenium against benzene toxicity in rats. Toxicol. Ind. Health. 27, 537-545.
  • Saha, S., Mukhopadhyay, M.K., Ghosh, P.D., Nath, D., Effect of methanolic leaf extract of Ocimum basilicum L. on benzene-induced hematotoxicity in mice. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine 2012; Article ID 176385, doi:10.1155/2012/176385.
  • Abd El Kader, M.A., El-Sammad, N.M., Fyiad, A.A., 2011. Effect of grape seeds extract in the modulation of matrix metalloproteinase-9 activity and oxidative stress induced by doxorubicin in mice. Life Sci. J. 8, 510-515.
  • Ahmed, A.A.E., Fatani, A.J., 2007. Protective effect of grape seeds proanthocyanidins against naphthalene-induced hepatotoxicity in rats. Saudi Pharm. J. 15, 38–47.
  • Raina, K., Singh, R.P., Agarwal, R., Agarwal, C., 2007. Oral grape seed extract inhibits prostate tumor growth and progression in TRAMP mice. Cancer Res. 67, 5976–5982.
  • Ariga, T., 2004. The antioxidative function, preventive action on disease and utilization of proanthocyanidins. Biofactors. 21, 197–201.
  • Bagchi, D., Bagchi, M., Stohs, S.J., Das, D.K., Ray, SD., Kuszynski, C.A., Joshi, S.S., 2000. Pruess HG: Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology. 148, 187–197.
  • Angelosanto, F.A., Blackburn, G.R., Schreiner, C.A., Mackerer, C.R., 1996. Benzene induces a dose-responsive increase in the frequency of micronucleated cells in rat Zymbal glands. Environ. Health Pers. 104, 1331-1336.
  • Yalçın, E., Oruç, E., Çavuşoğlu, K., Yapar, K., 2010. Protective role of grape seed extract against doxorubicin-induced cardiotoxicity and genotoxicity in albino mice. J. Med. Food. 13, 917-9
  • Te-Hsiu, M.A., Zhou, X., Loarco, G.F., Arreola, G.G., Lecona, S.U., 1995. Mouseerythrocyte micronucleus (MUS-EMN) assay on the clastogenicity of industrial wastewater. Rev. Int. Contam. Ambient. 11, 95–98.
  • Titenko-Holland, N., Windham, G., Kolachana, P., Reinisch, F., Parvatham, S., Osorio, A.M., Smith, M.T., 1997. Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mutat. Res. 388, 85–95.
  • Ozkul, Y., Donmez, H., Erenmemisoglu, A., Demirtas, H., Imamoglu, N., 1997. Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users. Mutagenesis. 12, 285–287.
  • Fenech, M., Chang, W.P., Kirsch-Volders, M., Holland, N., Bonassi, S., Zeiger, E., 2003. Human micronucleus project. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 534, 65–75.
  • Beyersmann, D., Hechtenberg, S., 1997. Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicol. Appl. Pharmacol. 144, 247–261.
  • Savage, J.R., 1976. Classification and relationships of induced chromosomal structural changes. J. Med. Genet. 13, 103–122.
  • Morgan, A.M., El-Tawil, O.S., 2006. Extract againist acrylonitrile-induced mutagenicity in rats. potential protective effect of grape seed. J. Egypt Soc. Toxicol. 34, 23-31.
  • Ahmed, A.A.E., Fatani, A.J., 2007. Protective effect of grape seeds proanthocyanidins against naphthalene-induced hepatotoxicity in rats. Saudi Pharm. J. 15, 38–47.
  • Zhao, Z., Wang, J., Chen, Y., Agarwal, R., 1999. Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiationpromotion protocol and identification of procyanidin B5-3-gallate as the most effective antioxidant constituent. Carcinogenesis. 20, 1737–1745.
  • Arii, M., Miki, M., Hosoyama, R., Ariga, H., Yamaji, N., Kataoka, S., 1998. Chemopreventive effect of grape seed extract on intestinal carcinogenesis in the APCMin mouse. Proc. Am. Assoc. Cancer Res. 39,20.
  • Raina, K., Singh, R.P., Agarwal, R., Agarwa,l C., 2007. Oral grape seed extract inhibits prostate tumor growth and progression in TRAMP mice. Cancer Res. 67, 5976–5982.
  • Hassan, N.S., Raafat, B.M., Aziz, S.W., 2010. Modulator role of grape seed extract on erythrocyte hemolysis and oxidative stress induced by microwave irradiation in rats. New York Sci J. 3, 66-73.
There are 29 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Editorial
Authors

Kültiğin Çavuşoğlu

Emine Yalçın This is me

Kürşad Yapar This is me

Birgül Gür This is me

Figen Çiçek This is me

Publication Date March 19, 2014
Published in Issue Year 2014 Volume: 35 Issue: 1

Cite

APA Çavuşoğlu, K., Yalçın, E., Yapar, K., Gür, B., et al. (2014). The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, 35(1), 1-11.
AMA Çavuşoğlu K, Yalçın E, Yapar K, Gür B, Çiçek F. The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. March 2014;35(1):1-11.
Chicago Çavuşoğlu, Kültiğin, Emine Yalçın, Kürşad Yapar, Birgül Gür, and Figen Çiçek. “The Protective Role of Grape Seed Extract Against Chronic Toxicity of Benzene in Swiss Albino Mice”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 35, no. 1 (March 2014): 1-11.
EndNote Çavuşoğlu K, Yalçın E, Yapar K, Gür B, Çiçek F (March 1, 2014) The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 35 1 1–11.
IEEE K. Çavuşoğlu, E. Yalçın, K. Yapar, B. Gür, and F. Çiçek, “The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice”, Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 35, no. 1, pp. 1–11, 2014.
ISNAD Çavuşoğlu, Kültiğin et al. “The Protective Role of Grape Seed Extract Against Chronic Toxicity of Benzene in Swiss Albino Mice”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 35/1 (March 2014), 1-11.
JAMA Çavuşoğlu K, Yalçın E, Yapar K, Gür B, Çiçek F. The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2014;35:1–11.
MLA Çavuşoğlu, Kültiğin et al. “The Protective Role of Grape Seed Extract Against Chronic Toxicity of Benzene in Swiss Albino Mice”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 35, no. 1, 2014, pp. 1-11.
Vancouver Çavuşoğlu K, Yalçın E, Yapar K, Gür B, Çiçek F. The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2014;35(1):1-11.