Research Article
BibTex RIS Cite

In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

Year 2022, Volume: 5 Issue: 1, 12 - 18, 30.06.2022
https://doi.org/10.38093/cupmap.1057290

Abstract

Lysimachia nummularia L. is a perennial medicinal and aromatic herb. In this study, the effects of solid and liquid culture environments on micropropagation of L. nummularia were investigated. Nodes were used as an explant type in the experiments. Different combinations of thidiazuron (TDZ: 0.05-0.80 mg L-1) and indole-3-butyric acid (IBA: 0.10 mg L-1) were used as growth regulators in Murashige and Skoog (MS) basal environment. Agar was put in to solidify the solid nutrient media. No agar was put in the liquid environment. In solid and liquid experiments, the most count of shoots was obtained in MS nutrient environment with 0.40 mg L-1 TDZ + 0.10 mg L-1 IBA. The longest shoot was detected in the nutrient environment including 0.05 mg L-1 TDZ + 0.10 mg L-1 IBA in both culture media. The best results for shoot count and percentage of regeneration were determined in the solid nutrient environment according to in vitro propagation values. On the other hand, the best results for shoot length were found in the liquid culture environment. The use of high TDZ in both culture media negatively affected shoot lengths. The regenerated plants were successfully acclimatized to aquatic conditions. The results of this study may help more efficient propagation of L. nummularia by tissue culture.

Supporting Institution

Technological Research Council of Turkey (TUBITAK)

Project Number

2130190

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant (Project no: 2130190).

References

  • 1. Ab Aziz, R., Kandasamy, K. I., Qamaruz Zaman, F., Namasivayam, P., (2021). In vitro shoot proliferation of Begonia pavonina: a comparison of semisolid, liquid, and temporary immersion medium system. Journal of Academia, 9, 39-48.
  • 2. Baskaran, P., Kumari, A., Van Staden, J., (2018). In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (Lf) Baker: a threatened medicinal plant. 3 Biotech, 8(1), 1-8.
  • 3. Beigmohamadi, M., Movafeghi, A., Jafari, S., Sharafi, A., (2021). Efficient in vitro organogenesis, micropropagation, and plumbagin production in Plumbago europaea L. In Vitro Cellular Developmental Biology-Plant, 57(5), 820-830.
  • 4. Dogan, M., (2018). In vitro micropropagation from nodal explants of the medicinal plant Lysimachia nummularia L. Journal Of Agriculture and Nature, 21(6), 875-881.
  • 5. Dogan, M., (2019a). Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.'nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian Journal of Biological and Chemical Sciences, 2(1), 11-16.
  • 6. Dogan, M., (2019b). Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29(2), 568-577.
  • 7. Emsen, B., Dogan, M., (2018). Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 17(1), 23-33.
  • 8. Gupta, V., Guleri, R., Gupta, M., Kaur, N., Kaur, K., Kumar, P., ... Pati, P.K., (2020). Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use. Plos ONE, 15(3), e0230142.
  • 9. Hamad, A. M., (2021). Effect of pH, Saccharose Concentrations and Medium States on in vitro Root-ing of Pineapple (Ananas comosus (L) Merr) cv Queen. Al-Mukhtar Journal of Sciences, 36(2), 135-147.
  • 10. Hussain, S. A., Ahmad, N., Anis, M., (2018). Synergetic effect of TDZ and BA on minimizing the post-exposure effects on axillary shoot proliferation and assessment of genetic fidelity in Rauvolfia tetraphylla (L.). Rendiconti Lincei. Scienze Fisiche e Naturali, 29(1), 109-115.
  • 11. Kala, C. P., (2015). Medicinal and aromatic plants: Boon for enterprise development. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 134-139.
  • 12. Khare, S. R., Kharate, P. S., kumar Sahu, R., Jha, Z., (2021). The rapid in-vitro micropropagation of Bamboo (Dendrocalamus strictus) and its genetic fidelity testing using ISSR markers. Environment Conservation Journal, 22(3), 69-77.
  • 13. Kodela, P., Jobson, R. W., (2016). Lysimachia nummularia (Primulaceae) Naturalised in New South Wales, Australia. Telopea, 19, 153-157.
  • 14. Lin, J., Zou, J., Zhang, B., Que, Q., Zhang, J., Chen, X., Zhou, W., (2021). An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent. Industrial Crops and Products, 170, 113686.
  • 15. Moniruzzaman, M., Yaakob, Z., Anuar, N., (2021). Factors affecting in vitro regeneration of Ficus carica L. and genetic fidelity studies using molecular marker. Journal of Plant Biochemistry and Biotechnology, 30(2), 304-316.
  • 16. Murashige, T., Skoog, F., (1962). A revised medium for rapid growth bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 473–497.
  • 17. Nithya, V., Kamalam, M., (2021). Standardization of a protocol for micropropagation of Eupatorium glandulosum L. an important medicinal plant. Plant Cell, Tissue and Organ Culture (PCTOC), 146, 339–344.
  • 18. Patricia, D., Stephen, B., John, A., (2021). Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook. f.) Skeels), an endangered medicinal plant. In Vitro Cellular Developmental Biology-Plant, 57(3), 493-498.
  • 19. Podolak, I., Koczurkiewicz, P., Michalik, M., Galanty, A., Zajdel, P., Janeczko, Z., (2013). A new cytotoxic triterpene saponin from Lysimachia nummularia L. Carbohydrate research, 375, 16-20.
  • 20. Rezali, N. I., Sidik, N. J., Saleh, A., Osman, N. I., Adam, N. A. M., (2017). The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pacific Journal of Tropical Biomedicine, 7(2), 151-156.
  • 21. Shekhawat, M. S., Kannan, N., Manokari, M., Ravindran, C. P., (2015). Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 174-181.
  • 22. Sivanesan, I., Saini, R. K., Noorzai, R., Zamany, A. J., Kim, D. H., (2016). In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge. 3 Biotech, 6(1), 91.
  • 23. Solomou, A. D., Martinos, K., Skoufogianni, E., Danalatos, N. G., (2016). Medicinal and aromatic plants diversity in Greece and their future prospects: A review. Agricultural Science, 4(1), 9-21.
  • 24. Tippani, R., Thammidala, C., (2021). TDZ induced plant regeneration from immature cotyledons of Pterocarpus marsupium Roxb. and validation of genetic homogeneity using ISSR markers. Vegetos, 34(1), 144-152.
  • 25. Yimam, T., (2018). Effect of solid and liquid media on in vitro propagation of Plectranthus edulis (Vatke) (Agnew). Journal of Biology, Agriculture and Healthcare, 8(3), 23-28.
Year 2022, Volume: 5 Issue: 1, 12 - 18, 30.06.2022
https://doi.org/10.38093/cupmap.1057290

Abstract

Project Number

2130190

References

  • 1. Ab Aziz, R., Kandasamy, K. I., Qamaruz Zaman, F., Namasivayam, P., (2021). In vitro shoot proliferation of Begonia pavonina: a comparison of semisolid, liquid, and temporary immersion medium system. Journal of Academia, 9, 39-48.
  • 2. Baskaran, P., Kumari, A., Van Staden, J., (2018). In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (Lf) Baker: a threatened medicinal plant. 3 Biotech, 8(1), 1-8.
  • 3. Beigmohamadi, M., Movafeghi, A., Jafari, S., Sharafi, A., (2021). Efficient in vitro organogenesis, micropropagation, and plumbagin production in Plumbago europaea L. In Vitro Cellular Developmental Biology-Plant, 57(5), 820-830.
  • 4. Dogan, M., (2018). In vitro micropropagation from nodal explants of the medicinal plant Lysimachia nummularia L. Journal Of Agriculture and Nature, 21(6), 875-881.
  • 5. Dogan, M., (2019a). Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.'nın boğum eksplantlarından çoklu sürgün rejenerasyonu. Eurasian Journal of Biological and Chemical Sciences, 2(1), 11-16.
  • 6. Dogan, M., (2019b). Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29(2), 568-577.
  • 7. Emsen, B., Dogan, M., (2018). Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Scientiarum Polonorum-Hortorum Cultus, 17(1), 23-33.
  • 8. Gupta, V., Guleri, R., Gupta, M., Kaur, N., Kaur, K., Kumar, P., ... Pati, P.K., (2020). Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use. Plos ONE, 15(3), e0230142.
  • 9. Hamad, A. M., (2021). Effect of pH, Saccharose Concentrations and Medium States on in vitro Root-ing of Pineapple (Ananas comosus (L) Merr) cv Queen. Al-Mukhtar Journal of Sciences, 36(2), 135-147.
  • 10. Hussain, S. A., Ahmad, N., Anis, M., (2018). Synergetic effect of TDZ and BA on minimizing the post-exposure effects on axillary shoot proliferation and assessment of genetic fidelity in Rauvolfia tetraphylla (L.). Rendiconti Lincei. Scienze Fisiche e Naturali, 29(1), 109-115.
  • 11. Kala, C. P., (2015). Medicinal and aromatic plants: Boon for enterprise development. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 134-139.
  • 12. Khare, S. R., Kharate, P. S., kumar Sahu, R., Jha, Z., (2021). The rapid in-vitro micropropagation of Bamboo (Dendrocalamus strictus) and its genetic fidelity testing using ISSR markers. Environment Conservation Journal, 22(3), 69-77.
  • 13. Kodela, P., Jobson, R. W., (2016). Lysimachia nummularia (Primulaceae) Naturalised in New South Wales, Australia. Telopea, 19, 153-157.
  • 14. Lin, J., Zou, J., Zhang, B., Que, Q., Zhang, J., Chen, X., Zhou, W., (2021). An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent. Industrial Crops and Products, 170, 113686.
  • 15. Moniruzzaman, M., Yaakob, Z., Anuar, N., (2021). Factors affecting in vitro regeneration of Ficus carica L. and genetic fidelity studies using molecular marker. Journal of Plant Biochemistry and Biotechnology, 30(2), 304-316.
  • 16. Murashige, T., Skoog, F., (1962). A revised medium for rapid growth bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 473–497.
  • 17. Nithya, V., Kamalam, M., (2021). Standardization of a protocol for micropropagation of Eupatorium glandulosum L. an important medicinal plant. Plant Cell, Tissue and Organ Culture (PCTOC), 146, 339–344.
  • 18. Patricia, D., Stephen, B., John, A., (2021). Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook. f.) Skeels), an endangered medicinal plant. In Vitro Cellular Developmental Biology-Plant, 57(3), 493-498.
  • 19. Podolak, I., Koczurkiewicz, P., Michalik, M., Galanty, A., Zajdel, P., Janeczko, Z., (2013). A new cytotoxic triterpene saponin from Lysimachia nummularia L. Carbohydrate research, 375, 16-20.
  • 20. Rezali, N. I., Sidik, N. J., Saleh, A., Osman, N. I., Adam, N. A. M., (2017). The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pacific Journal of Tropical Biomedicine, 7(2), 151-156.
  • 21. Shekhawat, M. S., Kannan, N., Manokari, M., Ravindran, C. P., (2015). Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 174-181.
  • 22. Sivanesan, I., Saini, R. K., Noorzai, R., Zamany, A. J., Kim, D. H., (2016). In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge. 3 Biotech, 6(1), 91.
  • 23. Solomou, A. D., Martinos, K., Skoufogianni, E., Danalatos, N. G., (2016). Medicinal and aromatic plants diversity in Greece and their future prospects: A review. Agricultural Science, 4(1), 9-21.
  • 24. Tippani, R., Thammidala, C., (2021). TDZ induced plant regeneration from immature cotyledons of Pterocarpus marsupium Roxb. and validation of genetic homogeneity using ISSR markers. Vegetos, 34(1), 144-152.
  • 25. Yimam, T., (2018). Effect of solid and liquid media on in vitro propagation of Plectranthus edulis (Vatke) (Agnew). Journal of Biology, Agriculture and Healthcare, 8(3), 23-28.
There are 25 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Muhammet Doğan 0000-0003-3138-5903

Project Number 2130190
Publication Date June 30, 2022
Published in Issue Year 2022 Volume: 5 Issue: 1

Cite

APA Doğan, M. (2022). In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium. Current Perspectives on Medicinal and Aromatic Plants, 5(1), 12-18. https://doi.org/10.38093/cupmap.1057290

-------------------------------------------------------------------------------------------------------------------------------

csm_neu_ezb_logo_670e8bf80b.jpg  Google_Scholar_logo_2015.PNG index_copernicus.jpg wclogo_block.png  logo.png  

Akademia_sosyal_bilimler_indeksi_logosu.gif  wide.png424-4243430_reviewers-for-these-journals-can-track-verify-and.png  orcid_logo.png?version=1&modificationDate=1473862307894&api=v2  1*mvsP194Golg0Dmo2rjJ-oQ.jpeg  aji.png citefactor-e1553074491226.png    logo1.jpg  semantci.png

-------------------------------------------------------------------------------------------------------------------------

88x31.png CUPMAP Journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

-----------------------------------------------------------------------------------------------------------------------------------------

Open_Access_PLoS.svg

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or  use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.