Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi

Sevinç Kıran; Ş.şebnem Ellialtıoğlu

doi:10.16882/derim.2019.611536

Research Article

Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi

Year 2019, Volume: 36 Issue: 2, 141 - 145, 10.12.2019

Sevinç Kıran Ş.şebnem Ellialtıoğlu

https://doi.org/10.16882/derim.2019.611536

Abstract

Bu çalışmanın amacı; Verticillium solgunluğuna dayanımın, bitki dokularında sentezlenen
fitoaleksin miktarıyla ilişkisini araştırmaktır. Çalışmada Long Purple patlıcan
çeşidi (hastalığa duyarlı) ile S. sisymbriifolium
yabani türü (hastalığa dayanıklı) bitkisel materyal olarak kullanılmıştır.
Elisitör olarak, araşidonik asit (5 ve 10 mM) çalışmada yer almıştır.
Elisitör ile uyarım işlemi 24, 48 ve 72 saat olarak uygulanmıştır. Ekstrakte
edilen maddeler gaz likit kromotografisi (GLC) cihazında analiz edilmiştir.
Dayanıklı türün kallus süspansiyon kültüründe fitoaleksin oluşumu yeterli
düzeyde uyarılamamıştır. Elde edilen bulgular araşidonik asidin kallus
süspansiyon kültüründe sadece Long Purple çeşitinde fitoaleksinlerin oluşumunu
uyarmada etkili olduğu, in vitro
koşularda doku fazında solavetivon ve süzüntü fazında ise lubimin maddelerinin
biriktiği belirlenmiştir. En yüksek madde birikimleri her iki fazda ‘10 mM
araşidonik asit x 72 saat uyarı süresi’ kombinasyonunda elde edilmiş, ‘doz x
uygulama süresi’ etkileşimi önemli bulunmuştur.

Keywords

Dayanıklılık, Kallus kültürü, Lubimin, Patlıcan, Solavetivon

Supporting Institution

Ankara Üniv. Fen Bilimleri Enst. (doktora tezi)

References

Ahmed, E.S., El- Essaway, A.A., Abou El-Hawa, M.E., Ezzat, S.M., & Metwaly, M.B. (1997). Biotic and abiotic initiators for rishitin formation and accumulation in tomato. Folia Microbiologica, 42(5):468-472.
Bletsos, F.A.,Stavropoulos, N.I., Papdopoulou, P.P., & Antonopoulou, P.D. (2004). Evaluation of eggplant (Solanum melongena L.) germplasm for resistance to Verticillium wilt. Advances in Horticultural Science, 18(1):33-37.
Bostock, R.M., & Kuc, J.A. (1981). Eicosapentaenoic and arachidonic acid from Phytophthora infestans elicit fungutoxic sesquiterpenoids in the potato. Science, 212(4490):67-69.
Desjardins, A.E., Mccormick, S.P., Plaisted, R.L., & Brodie, B.B. (1997). Association between solavetivone production and resistance to Globodera rostochiensis in potato. Journal of Agricultural and Food Chemistry, 45(6):2322-2326.
D'harlinque, A., Mamdouh, A.M., Malfatti, P., Souline, M.C., & Bombeix, G. (1995). Evidence for rishitin biosynthesis in tomato cultures. Phytochemistry, 39(1):69-70.
Ebel, J., Cosio, E.G., Grab, D., & Habereder, H. (1989). Stimulation of phytoalexin accumulation in fungus-infected roots and elicitor-treated cell cultures of soybean (Glycine max L.). Primary and Secondary Metabolism of Plant Cell Cultures II (ed: Kurz, W.G.W.), p. 229-236. Berlin, Springer Verlag.
Elekçioğlu, I.H., Canihoş, Y., Özgönen, H., Söğüt, M.A., Albajes, R., & Şekeroğlu, E. (2000). Induction of resistance on eggplants against Verticillium wilt disease and root-knot nematodes using biotic and abiotic factors. Bulletin OILB-SROP, 23(1):63-69.
Freed, R., Einensmith, S.P., Guets, S., Reicosky, D., Smail, V.W., & Wolberg, P. (1989). User’s guide to MSTAT-C, an analysis of agronomic research experiment. Michigan State University, USA.
González-Lamothe, R., Mitchell, G., Gattuso, M., Diarra, M.S., Malouin, F., & Bouarab, K. (2009). Plant antimicrobial agents and their effects on plant and human pathogens. International Journal of Molecular Sciences,10(8):3400-3419.
Hoshino, T., Chida, M., Yamaura, T., Yoshizaura, Y., & Mizutani, J. (1994). Phytoalexin induction in green pepper cell cultures treated with arachidonic acid. Phytochemistry, 36(6):1417-1419.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiology Plantarum, 15(3):473-497.
Nagaoka, T., Goto, K., Watanabe, A., Sakata, Y., & Yoshihara, T. (2001). Sesquiterpenoids in root exudates of Solanum aethiopicum. Zeitschrift für Naturforschung. Section C Biosciences, 56(9-10):707-713.
Ozeretskovskaya, O.L., Varlamov, V.P., Vasyukova, N.I., Chalenko, G.I., Gerasimova, N.G., & Panina, Y.S. (2004). Influence of systemic signal molecules on the rate of spread of the immunizing effect of elicitors over potato tissues. Applied Biochemistry and Microbiology. 40(2):213-216.
Raaymakers, T.M., & Van den Ackerveken, G. (2015). Extracellular recognition of oomycetes during biotrophic infection of plants. Frontiers in Plant Science, 7:906. doi: 10.3389/fpls.2016.00906.
Robinson, R.W., Shail, J.W., Gao, Y., & Doganlar, S. (2000). Interspecific hybridization of eggplant for Verticillium wilt resistance and other useful traits. http:// bgard-sci.kund.nl/eggnet/egginfo. Erişim tarihi: 01.09.2003.
Rozhnova, N.A., Gerashchenkov, G.A., & Babosha, A.V. (2003). The effect of arachidonic acid and viral infection on the phytohemagglutinin activity during the development of tobacco acquired resistance. Russian Journal of Plant Physiology, 50(5): 661-665.
Sevin, M., Sevin, N., Uslu Kıran, S., & Ellialtıoğlu, Ş. (2010). Determination of relation between resistance to Verticillium dahliae Kleb. and phytoalexins accumulation in some Solanum species (in Turkish). Proceedings of the VIII. National Vegetable Growing Symposium, Van, Turkey. p: 444-447.
Uslu Kıran, S., Ellialtıoğlu, Ş., Dolar, F.S., Üstün, A.S., Mehmetoğlu, Ü., & Bayraktar, H. (2007). Patlıcanda Verticillium solgunluğuna dayanıklılık ile kallus kültüründe solavetivon birikimi arasındaki ilişkinin incelenmesi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(1):45-53.
Uslu Kıran, S., Ellialtıoğlu, Ş., Mehmetoğlu, & Ü., Üstün, S. (2010). Patlıcandaki bazı fitoaleksinlerin hastalıklara dayanıklılık özelliği ile ilişkileri ve analiz yöntemleri. VIII.Sebze Tarımı Sempozyumu, 23-26 Haziran 2010, Van. Bildiri Kitabı (Eds: Yaşar, F., Çavuşoğlu, Ş., Biçim, M.), s. 448-454.
Yakose, T., Kamoto, K., Park, S., Matsuura, H., & Yoshihara, T. (2004). Anti-fungal sesquiterpenoid from the root exudate of Solanum abutiloides. Bioscience Biotechnology and Biochemistry, 68(12): 2640-2642.
Zhang, W.W., Wang, S.Z., Liu, K., Si, N., Qi, F.J., & Jian, G.J. (2012). Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Physiological and Molecular Plant Pathology, 80: 50-57 doi.org/10.1016/j.pmpp.2012.09.001

The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture

Year 2019, Volume: 36 Issue: 2, 141 - 145, 10.12.2019

Sevinç Kıran Ş.şebnem Ellialtıoğlu

https://doi.org/10.16882/derim.2019.611536

Abstract

The
aim of this study was to investigate the relationship between the resistance of
Verticillium wilt and the amount of
phytoalexin, which is the defense molecules synthesized in plant tissues. In
this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (disease
resistant) were used as plant material. As elicitor, arachidonic acid (5 and
10 mM) was included in the study. Stimulation with elicitor lasted 24, 48
and 72 hours. Extracted substances were analyzed in gas liquid chromatography
(GLC). The formation of phytoalexin was not adequately stimulated in the callus
suspension culture of the resistant species. The results showed that
arachidonic acid was effective in stimulating the formation of phytoalexins in
the callus suspension culture in Long Purple variety, and in the in vitro conditions solavetivone was
formed in the tissue phase and lubimin substances in the filtrate
phase. The highest material accumulations were obtained in both phases:
’10 mM arachidonic acid x 72 hours’ ‘elicitor dose x stimulation period’
interaction was found to be significant.

Keywords

Callus culture, Eggplant, Lubimin, Resistance, Solavetivone

References

Ahmed, E.S., El- Essaway, A.A., Abou El-Hawa, M.E., Ezzat, S.M., & Metwaly, M.B. (1997). Biotic and abiotic initiators for rishitin formation and accumulation in tomato. Folia Microbiologica, 42(5):468-472.
Bletsos, F.A.,Stavropoulos, N.I., Papdopoulou, P.P., & Antonopoulou, P.D. (2004). Evaluation of eggplant (Solanum melongena L.) germplasm for resistance to Verticillium wilt. Advances in Horticultural Science, 18(1):33-37.
Bostock, R.M., & Kuc, J.A. (1981). Eicosapentaenoic and arachidonic acid from Phytophthora infestans elicit fungutoxic sesquiterpenoids in the potato. Science, 212(4490):67-69.
Desjardins, A.E., Mccormick, S.P., Plaisted, R.L., & Brodie, B.B. (1997). Association between solavetivone production and resistance to Globodera rostochiensis in potato. Journal of Agricultural and Food Chemistry, 45(6):2322-2326.
D'harlinque, A., Mamdouh, A.M., Malfatti, P., Souline, M.C., & Bombeix, G. (1995). Evidence for rishitin biosynthesis in tomato cultures. Phytochemistry, 39(1):69-70.
Ebel, J., Cosio, E.G., Grab, D., & Habereder, H. (1989). Stimulation of phytoalexin accumulation in fungus-infected roots and elicitor-treated cell cultures of soybean (Glycine max L.). Primary and Secondary Metabolism of Plant Cell Cultures II (ed: Kurz, W.G.W.), p. 229-236. Berlin, Springer Verlag.
Elekçioğlu, I.H., Canihoş, Y., Özgönen, H., Söğüt, M.A., Albajes, R., & Şekeroğlu, E. (2000). Induction of resistance on eggplants against Verticillium wilt disease and root-knot nematodes using biotic and abiotic factors. Bulletin OILB-SROP, 23(1):63-69.
Freed, R., Einensmith, S.P., Guets, S., Reicosky, D., Smail, V.W., & Wolberg, P. (1989). User’s guide to MSTAT-C, an analysis of agronomic research experiment. Michigan State University, USA.
González-Lamothe, R., Mitchell, G., Gattuso, M., Diarra, M.S., Malouin, F., & Bouarab, K. (2009). Plant antimicrobial agents and their effects on plant and human pathogens. International Journal of Molecular Sciences,10(8):3400-3419.
Hoshino, T., Chida, M., Yamaura, T., Yoshizaura, Y., & Mizutani, J. (1994). Phytoalexin induction in green pepper cell cultures treated with arachidonic acid. Phytochemistry, 36(6):1417-1419.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiology Plantarum, 15(3):473-497.
Nagaoka, T., Goto, K., Watanabe, A., Sakata, Y., & Yoshihara, T. (2001). Sesquiterpenoids in root exudates of Solanum aethiopicum. Zeitschrift für Naturforschung. Section C Biosciences, 56(9-10):707-713.
Ozeretskovskaya, O.L., Varlamov, V.P., Vasyukova, N.I., Chalenko, G.I., Gerasimova, N.G., & Panina, Y.S. (2004). Influence of systemic signal molecules on the rate of spread of the immunizing effect of elicitors over potato tissues. Applied Biochemistry and Microbiology. 40(2):213-216.
Raaymakers, T.M., & Van den Ackerveken, G. (2015). Extracellular recognition of oomycetes during biotrophic infection of plants. Frontiers in Plant Science, 7:906. doi: 10.3389/fpls.2016.00906.
Robinson, R.W., Shail, J.W., Gao, Y., & Doganlar, S. (2000). Interspecific hybridization of eggplant for Verticillium wilt resistance and other useful traits. http:// bgard-sci.kund.nl/eggnet/egginfo. Erişim tarihi: 01.09.2003.
Rozhnova, N.A., Gerashchenkov, G.A., & Babosha, A.V. (2003). The effect of arachidonic acid and viral infection on the phytohemagglutinin activity during the development of tobacco acquired resistance. Russian Journal of Plant Physiology, 50(5): 661-665.
Sevin, M., Sevin, N., Uslu Kıran, S., & Ellialtıoğlu, Ş. (2010). Determination of relation between resistance to Verticillium dahliae Kleb. and phytoalexins accumulation in some Solanum species (in Turkish). Proceedings of the VIII. National Vegetable Growing Symposium, Van, Turkey. p: 444-447.
Uslu Kıran, S., Ellialtıoğlu, Ş., Dolar, F.S., Üstün, A.S., Mehmetoğlu, Ü., & Bayraktar, H. (2007). Patlıcanda Verticillium solgunluğuna dayanıklılık ile kallus kültüründe solavetivon birikimi arasındaki ilişkinin incelenmesi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(1):45-53.
Uslu Kıran, S., Ellialtıoğlu, Ş., Mehmetoğlu, & Ü., Üstün, S. (2010). Patlıcandaki bazı fitoaleksinlerin hastalıklara dayanıklılık özelliği ile ilişkileri ve analiz yöntemleri. VIII.Sebze Tarımı Sempozyumu, 23-26 Haziran 2010, Van. Bildiri Kitabı (Eds: Yaşar, F., Çavuşoğlu, Ş., Biçim, M.), s. 448-454.
Yakose, T., Kamoto, K., Park, S., Matsuura, H., & Yoshihara, T. (2004). Anti-fungal sesquiterpenoid from the root exudate of Solanum abutiloides. Bioscience Biotechnology and Biochemistry, 68(12): 2640-2642.
Zhang, W.W., Wang, S.Z., Liu, K., Si, N., Qi, F.J., & Jian, G.J. (2012). Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Physiological and Molecular Plant Pathology, 80: 50-57 doi.org/10.1016/j.pmpp.2012.09.001

There are 21 citations in total.

Details

Primary Language	Turkish
Journal Section	Research Articles
Authors	Sevinç Kıran 0000-0002-6756-0235 Ş.şebnem Ellialtıoğlu This is me 0000-0002-3851-466X
Publication Date	December 10, 2019
Published in Issue	Year 2019 Volume: 36 Issue: 2

Cite

APA	Kıran, S., & Ellialtıoğlu, Ş. (2019). Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi. Derim, 36(2), 141-145. https://doi.org/10.16882/derim.2019.611536
AMA	Kıran S, Ellialtıoğlu Ş. Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi. DERİM. December 2019;36(2):141-145. doi:10.16882/derim.2019.611536
Chicago	Kıran, Sevinç, and Ş.şebnem Ellialtıoğlu. “Araşidonik Asidin patlıcan Kallus süspansiyon kültüründe Fitoaleksin uyartımı üzerine Etkisi”. Derim 36, no. 2 (December 2019): 141-45. https://doi.org/10.16882/derim.2019.611536.
EndNote	Kıran S, Ellialtıoğlu Ş (December 1, 2019) Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi. Derim 36 2 141–145.
IEEE	S. Kıran and Ş. Ellialtıoğlu, “Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi”, DERİM, vol. 36, no. 2, pp. 141–145, 2019, doi: 10.16882/derim.2019.611536.
ISNAD	Kıran, Sevinç - Ellialtıoğlu, Ş.şebnem. “Araşidonik Asidin patlıcan Kallus süspansiyon kültüründe Fitoaleksin uyartımı üzerine Etkisi”. Derim 36/2 (December 2019), 141-145. https://doi.org/10.16882/derim.2019.611536.
JAMA	Kıran S, Ellialtıoğlu Ş. Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi. DERİM. 2019;36:141–145.
MLA	Kıran, Sevinç and Ş.şebnem Ellialtıoğlu. “Araşidonik Asidin patlıcan Kallus süspansiyon kültüründe Fitoaleksin uyartımı üzerine Etkisi”. Derim, vol. 36, no. 2, 2019, pp. 141-5, doi:10.16882/derim.2019.611536.
Vancouver	Kıran S, Ellialtıoğlu Ş. Araşidonik asidin patlıcan kallus süspansiyon kültüründe fitoaleksin uyartımı üzerine etkisi. DERİM. 2019;36(2):141-5.