Research Article
Katı ve Sıvı Kültür Ortamlarında LED Aydınlatmanın Alternanthera reineckii Briq.'nin Mikroçoğaltımı Üzerine Etkisi
Abstract
Işık, bitki büyümesinde önemli çevresel faktörlerden biridir. Birçok avantajından dolayı ışık yayan diyotlar (LED), bitkilerin ışık ortamının düzenlenmesi için önemli ışık kaynaklarından biri haline gelmiştir. Bu çalışmada, Thidiazuron (TDZ) içeren katı ve sıvı kültür ortamları ve farklı LED aydınlatma koşullarının Alternanthera reineckii Briq.’nin sürgün rejenerasyon yeteneği üzerindeki etkileri incelenmiştir. Boğum eksplantları 0,25-1 mg/L TDZ içeren Murashige ve Skoog (MS) besin ortamında kültüre alınmıştır. Aydınlatma için kırmızı, mavi ve beyaz LED tek olarak ve kırmızı ve mavi LED ışıkların farklı kombinasyonları kullanılmıştır. Kontrol olarak beyaz floresan tercih edilmiştir. Sürgün rejenerasyon değerleri incelendiğinde, LED ışıkların flouresan ışığa göre daha verimli oldukları görülmüştür. Daha fazla sürgün sayısı ve uzun sürgünler kırmızı ve mavi LED ışıkların kombinasyonlarında elde edilmiştir. 0,25 mg/L TDZ ve 1K:2M LED ışık uygulamaları katı kültür ortamında en fazla sayıda sürgünleri vermiştir (9,03 sürgün/eksplant). Sıvı kültür ortamında en fazla sürgün sayısı 0,50 mg/L TDZ ve 2K:1M LED uygulamalarında elde edilmiştir (11,62 sürgün/eksplant). Katı ve sıvı kültürlerde en uzun sürgünler 1K:2M LED ışık altında ve 0,25 mg/L TDZ’li MS besin ortamında tespit edilmiştir. Doku kültürü koşullarında köklendirlen bitkiler ardından akvaryum koşullarına başarışı şekilde alıştırılmıştır.
Supporting Institution
Karamanoğlu Mehmetbey Üniversitesi, Bilimsel Araştırma Projeleri Komisyonu
Project Number
12-YL-20
Thanks
Bu çalışma, Karamanoğlu Mehmetbey Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Tarafından Desteklenmiştir (Proje No: 12-YL-20).
References
- Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., ... Dobránszki, J. (2022). An academic and technical overview on plant micropropagation challenges. Horticulturae, 8(8), 677.
- Acemi, A., Bayrak, B., Çakır, M., Demiryürek, E., Gün, E., El Gueddari, N. E., … Özen, F. (2018). Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cellular & Developmental Biology-Plant, 54, 537-544.
- Al Murad, M., Razi, K., Jeong, B. R., Samy, P. M. A., & Muneer, S. (2021). Light emitting diodes (LEDs) as agricultural lighting: Impact and its potential on improving physiology, flowering, and secondary metabolites of crops. Sustainability, 13(4), 1985.
- Araújo, R. C., Rodrigues, F. A., Dória, J., & Pasqual, M. (2022). In vitro germination of Adenium obesum under the effects of culture medium and light emitting diodes of different colors. Plant Cell, Tissue and Organ Culture, 149(3), 523-533.
- Bello, A. M., Kamali Aliabad, K., Saravi, A. T., & Sodaei Zade, H. (2022). Determination of the Best Culture Medium and plant growth regulators for micropropagation of Neem Tree (Azadirachta indica A. Juss). International Journal of Horticultural Science and Technology, 9(2), 237-245.
- Choudhary, A. K., Kumari, P., & Kumari, S. (2022). In vitro propagation of two commercially important bamboo species (Bambusa tulda Roxb. and Dendrocalamus stocksii Munro.). African Journal of Biotechnology, 21(2), 83-94.
- Cioć, M., Szewczyk, A., Żupnik, M., Kalisz, A., & Pawłowska, B. (2018). LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell, Tissue and Organ Culture (PCTOC), 132, 433-447.
- de Jesús Romo-Paz, F., Folgado, R., Delgado-Aceves, L., Zamora-Natera, J. F., & Portillo, L. (2021). Tissue culture of Physalis angulata L.(Solanaceae): techniques for micropropagation and germplasm long-term preservation. Plant Cell, Tissue and Organ Culture (PCTOC), 144, 73-78.
- Dinçer, D., Bekiryazıcı, F., Dündar, H., & Ögçe, H. (2023). Germination and Micropropagation of Crataegus monogyna Jacq. Seeds by Tissue Culture Method. Forest Science, 69(2), 178-186.
- Djangalina, E. D., Kapytina, A. I., Kaigermazova, M. A., Mamirova, A. A., & Shadenova, E. A. (2023). Influence of light-emitting diodes on the efficiency of valuable woody plants micropropagation. International Journal of Biology and Chemistry, 16(1), 49-57.
- Dogan, M. (2020). The effectiveness of light emitting diodes on shoot regeneration in vitro from shoot tip tissues of Limnophila aromatica (Lamk.) Merr. and Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Biotechnic & Histochemistry, 95(3), 225-232.
- Dogan, M. (2022). In vitro micropropagation of Pogostemon erectus (Dalzell) Kuntze in liquid culture medium. Natural and Engineering Sciences, 7(1), 80-88.
- Dogan, M., & Emsen, B. (2018). Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian Journal of Biological and Chemical Sciences, 1(2), 48-53.
- Gupta, R., & Sood, H. (2023). Emerging technologies for the production of in vitro raised quality rich Swertia chirayita by using led lights. Sustainability, 15(2), 1714.
- Haque, M. I., Singh, P. K., Ghuge, S., Kumar, A., Rai, A. C., Kumar, A., & Modi, A. (2022). A general introduction to and background of plant tissue culture: Past, current, and future aspects. In Advances in Plant Tissue Culture (pp. 1-30). Academic Press.
- Ioannidis, K., Tomprou, I., Panayiotopoulou, D., Boutsios, S., & Daskalakou, E. N. (2023). Potential and constraints on in vitro micropropagation of Juniperus drupacea Labill. Forests, 14(1), 142.
- Kamal, K. Y., Khodaeiaminjan, M., El‐Tantawy, A. A., Moneim, D. A., Salam, A. A., Ash‐shormillesy, S. M., ... Ramadan, M. F. (2020). Evaluation of growth and nutritional value of Brassica microgreens grown under red, blue and green LEDs combinations. Physiologia Plantarum, 169(4), 625-638.
- Kose, M. S. H., Dogan, M., & Sadi, G. (2021). Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze. Biologia, 76(3), 1053-1061.
- Kumar, A., Chauhan, S., Rattan, S., Warghat, A. R., Kumar, D., & Bhargava, B. (2022). In vitro propagation and phyto-chemical assessment of Cymbidium aloifolium (L.) Sw.: An orchid of pharma-horticultural importance. South African Journal of Botany, 144, 261-269.
- Lim, M. J., Murthy, H. N., Song, H. Y., Lee, S. Y., & Park, S. Y. (2023). Influence of white, red, blue, and combination of led lights on in vitro multiplication of shoots, rooting, and acclimatization of Gerbera jamesonii cv.‘Shy Pink’Plants. Agronomy, 13(9), 2216.
- Longchar, T. B., & Deb, C. R. (2022). Optimization of in vitro propagation protocol of Dendrobium heterocarpum Wall. ex. Lindl. and clonal genetic fidelity assessment of the regenerates: An orchid of horticultural and medicinal importance. South African Journal of Botany, 149, 67-78.
- Martinez, L. A. M., & Andreu, L. G. I. (2022). Effect of LED lights on the in vitro growth of Pinus pseudostrobus Lindl., plants. Journal of Forest Science, 68(8), 311-317.
- Martínez-Estrada, E., Caamal-Velázquez, J. H., Morales-Ramos, V., & Bello-Bello, J. J. (2016). Light emitting diodes improve in vitro shoot multiplication and growth of Anthurium andreanum Lind. Propagation of Ornamental Plants, 16(1), 3-8.
- Mascarenhas, L. M. S., Santana, J. R. F. D., & Brito, A. L. (2019). Micropropagation of Physalis peruviana L. Pesquisa Agropecuária Tropical, 49, e55603.
- Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
- Oseni, O. M., Nailwal, T. K., & Pande, V. (2022). Callus induction and multiple shoot proliferation from nodal explants of Mansonia altissima: confirmation of genetic stability using ISSR and RAPD markers. In Vitro Cellular & Developmental Biology-Plant, 58(3), 479-488.
- Papafotiou, M., Vlachou, G., & Martini, A. N. (2023). Investigation of the effects of the explant type and different plant growth regulators on micropropagation of five mediterranean Salvia spp. Native to Greece. Horticulturae, 9(1), 96.
- Patil, A. M., Gunjal, P. P., & Das, S. (2021). In vitro micropropagation of Lilium candidum bulb by application of multiple hormone concentrations using plant tissue culture technique. International Journal for Research in Applied Sciences and Biotechnology, 8(2), 244-253.
- Schuerger, A. C., Brown, C. S., & Stryjewski, E. C. (1997). Anatomical features of pepper plants (Capsicum annuumL.) grown under red light-emitting diodes supplemented with blue or far-red light. Annals of Botany, 79(3), 273-282.
- Singh, A. (2015). Micropropagation of plants. Plant biology and biotechnology: volume II: plant genomics and biotechnology, 329-346.
- Türker, A. H., & Hatipoğlu, R. (2018). Dağ kekiği (Origanum syriacum L. var. bevanii (Holmes) Ietswaart)’nin mikroçoğaltımı. Ormancılık Araştırma Dergisi, 5(2), 97-111.
- Uğur, K., Doğan, M., & Kaya, A. (2019). Alternanthera reineckii Briq.’nin doku kültürü çalışmaları için yüzey sterilizasyonunun optimizasyonu. Eurasian Journal of Biological and Chemical Sciences, 2(1), 24-28.
- Warhade, M. I., & Badere, R. S. (2017). Seasonal variation in the shoot-regeneration potential of the nodal explants of Rosa setigera. The Journal of Indian Botanical Society, 96(3-4), 188-197.
- Xu, Y., Liang, Y., & Yang, M. (2019). Effects of composite LED light on root growth and antioxidant capacity of Cunninghamia lanceolata tissue culture seedlings. Scientific reports, 9(1), 9766.
Influence of LED Lighting on Micropropagation of Alternanthera reineckii Briq. in Solid and Liquid Culture Media
Abstract
The light is one of the important environmental factors in plant growth. Due to its many advantages, light emitting diodes (LEDs) have become one of the important light sources for regulating the light environment of plants. In this study, the effects of solid and liquid culture media containing Thidiazuron (TDZ) and different LED lighting conditions on the shoot regeneration ability of Alternanthera reineckii Briq. Nodal explants were cultured in Murashige and Skoog (MS) medium including 0.25-1 mg/L TDZ. Single red, blue and white LEDs and different combinations of red and blue LEDs are used for illumination. White fluorescent was used as a control. When the shoot regeneration values were examined, it was seen that LED lights were more efficient than fluorescent light. Higher shoot numbers and longer shoots were obtained in combinations of red and blue LED lights. 0.25 mg/L TDZ and 1R:2B LED light treatments gave the highest number of shoots in solid culture medium (9.03 shoots/explants). The maximum number of shoots in liquid culture medium was obtained in 0.50 mg/L TDZ and 2K:1M LED applications (11.62 shoots/explants). The longest shoots in solid and liquid cultures were determined under 1R:2B LED light and in MS nutrient medium with 0.25 mg/L TDZ. The plants rooted in tissue culture conditions were then successfully acclimatized to aquarium conditions.
Project Number
12-YL-20
References
- Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., ... Dobránszki, J. (2022). An academic and technical overview on plant micropropagation challenges. Horticulturae, 8(8), 677.
- Acemi, A., Bayrak, B., Çakır, M., Demiryürek, E., Gün, E., El Gueddari, N. E., … Özen, F. (2018). Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cellular & Developmental Biology-Plant, 54, 537-544.
- Al Murad, M., Razi, K., Jeong, B. R., Samy, P. M. A., & Muneer, S. (2021). Light emitting diodes (LEDs) as agricultural lighting: Impact and its potential on improving physiology, flowering, and secondary metabolites of crops. Sustainability, 13(4), 1985.
- Araújo, R. C., Rodrigues, F. A., Dória, J., & Pasqual, M. (2022). In vitro germination of Adenium obesum under the effects of culture medium and light emitting diodes of different colors. Plant Cell, Tissue and Organ Culture, 149(3), 523-533.
- Bello, A. M., Kamali Aliabad, K., Saravi, A. T., & Sodaei Zade, H. (2022). Determination of the Best Culture Medium and plant growth regulators for micropropagation of Neem Tree (Azadirachta indica A. Juss). International Journal of Horticultural Science and Technology, 9(2), 237-245.
- Choudhary, A. K., Kumari, P., & Kumari, S. (2022). In vitro propagation of two commercially important bamboo species (Bambusa tulda Roxb. and Dendrocalamus stocksii Munro.). African Journal of Biotechnology, 21(2), 83-94.
- Cioć, M., Szewczyk, A., Żupnik, M., Kalisz, A., & Pawłowska, B. (2018). LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell, Tissue and Organ Culture (PCTOC), 132, 433-447.
- de Jesús Romo-Paz, F., Folgado, R., Delgado-Aceves, L., Zamora-Natera, J. F., & Portillo, L. (2021). Tissue culture of Physalis angulata L.(Solanaceae): techniques for micropropagation and germplasm long-term preservation. Plant Cell, Tissue and Organ Culture (PCTOC), 144, 73-78.
- Dinçer, D., Bekiryazıcı, F., Dündar, H., & Ögçe, H. (2023). Germination and Micropropagation of Crataegus monogyna Jacq. Seeds by Tissue Culture Method. Forest Science, 69(2), 178-186.
- Djangalina, E. D., Kapytina, A. I., Kaigermazova, M. A., Mamirova, A. A., & Shadenova, E. A. (2023). Influence of light-emitting diodes on the efficiency of valuable woody plants micropropagation. International Journal of Biology and Chemistry, 16(1), 49-57.
- Dogan, M. (2020). The effectiveness of light emitting diodes on shoot regeneration in vitro from shoot tip tissues of Limnophila aromatica (Lamk.) Merr. and Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Biotechnic & Histochemistry, 95(3), 225-232.
- Dogan, M. (2022). In vitro micropropagation of Pogostemon erectus (Dalzell) Kuntze in liquid culture medium. Natural and Engineering Sciences, 7(1), 80-88.
- Dogan, M., & Emsen, B. (2018). Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian Journal of Biological and Chemical Sciences, 1(2), 48-53.
- Gupta, R., & Sood, H. (2023). Emerging technologies for the production of in vitro raised quality rich Swertia chirayita by using led lights. Sustainability, 15(2), 1714.
- Haque, M. I., Singh, P. K., Ghuge, S., Kumar, A., Rai, A. C., Kumar, A., & Modi, A. (2022). A general introduction to and background of plant tissue culture: Past, current, and future aspects. In Advances in Plant Tissue Culture (pp. 1-30). Academic Press.
- Ioannidis, K., Tomprou, I., Panayiotopoulou, D., Boutsios, S., & Daskalakou, E. N. (2023). Potential and constraints on in vitro micropropagation of Juniperus drupacea Labill. Forests, 14(1), 142.
- Kamal, K. Y., Khodaeiaminjan, M., El‐Tantawy, A. A., Moneim, D. A., Salam, A. A., Ash‐shormillesy, S. M., ... Ramadan, M. F. (2020). Evaluation of growth and nutritional value of Brassica microgreens grown under red, blue and green LEDs combinations. Physiologia Plantarum, 169(4), 625-638.
- Kose, M. S. H., Dogan, M., & Sadi, G. (2021). Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze. Biologia, 76(3), 1053-1061.
- Kumar, A., Chauhan, S., Rattan, S., Warghat, A. R., Kumar, D., & Bhargava, B. (2022). In vitro propagation and phyto-chemical assessment of Cymbidium aloifolium (L.) Sw.: An orchid of pharma-horticultural importance. South African Journal of Botany, 144, 261-269.
- Lim, M. J., Murthy, H. N., Song, H. Y., Lee, S. Y., & Park, S. Y. (2023). Influence of white, red, blue, and combination of led lights on in vitro multiplication of shoots, rooting, and acclimatization of Gerbera jamesonii cv.‘Shy Pink’Plants. Agronomy, 13(9), 2216.
- Longchar, T. B., & Deb, C. R. (2022). Optimization of in vitro propagation protocol of Dendrobium heterocarpum Wall. ex. Lindl. and clonal genetic fidelity assessment of the regenerates: An orchid of horticultural and medicinal importance. South African Journal of Botany, 149, 67-78.
- Martinez, L. A. M., & Andreu, L. G. I. (2022). Effect of LED lights on the in vitro growth of Pinus pseudostrobus Lindl., plants. Journal of Forest Science, 68(8), 311-317.
- Martínez-Estrada, E., Caamal-Velázquez, J. H., Morales-Ramos, V., & Bello-Bello, J. J. (2016). Light emitting diodes improve in vitro shoot multiplication and growth of Anthurium andreanum Lind. Propagation of Ornamental Plants, 16(1), 3-8.
- Mascarenhas, L. M. S., Santana, J. R. F. D., & Brito, A. L. (2019). Micropropagation of Physalis peruviana L. Pesquisa Agropecuária Tropical, 49, e55603.
- Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.
- Oseni, O. M., Nailwal, T. K., & Pande, V. (2022). Callus induction and multiple shoot proliferation from nodal explants of Mansonia altissima: confirmation of genetic stability using ISSR and RAPD markers. In Vitro Cellular & Developmental Biology-Plant, 58(3), 479-488.
- Papafotiou, M., Vlachou, G., & Martini, A. N. (2023). Investigation of the effects of the explant type and different plant growth regulators on micropropagation of five mediterranean Salvia spp. Native to Greece. Horticulturae, 9(1), 96.
- Patil, A. M., Gunjal, P. P., & Das, S. (2021). In vitro micropropagation of Lilium candidum bulb by application of multiple hormone concentrations using plant tissue culture technique. International Journal for Research in Applied Sciences and Biotechnology, 8(2), 244-253.
- Schuerger, A. C., Brown, C. S., & Stryjewski, E. C. (1997). Anatomical features of pepper plants (Capsicum annuumL.) grown under red light-emitting diodes supplemented with blue or far-red light. Annals of Botany, 79(3), 273-282.
- Singh, A. (2015). Micropropagation of plants. Plant biology and biotechnology: volume II: plant genomics and biotechnology, 329-346.
- Türker, A. H., & Hatipoğlu, R. (2018). Dağ kekiği (Origanum syriacum L. var. bevanii (Holmes) Ietswaart)’nin mikroçoğaltımı. Ormancılık Araştırma Dergisi, 5(2), 97-111.
- Uğur, K., Doğan, M., & Kaya, A. (2019). Alternanthera reineckii Briq.’nin doku kültürü çalışmaları için yüzey sterilizasyonunun optimizasyonu. Eurasian Journal of Biological and Chemical Sciences, 2(1), 24-28.
- Warhade, M. I., & Badere, R. S. (2017). Seasonal variation in the shoot-regeneration potential of the nodal explants of Rosa setigera. The Journal of Indian Botanical Society, 96(3-4), 188-197.
- Xu, Y., Liang, Y., & Yang, M. (2019). Effects of composite LED light on root growth and antioxidant capacity of Cunninghamia lanceolata tissue culture seedlings. Scientific reports, 9(1), 9766.
There are 34 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Botany (Other) |
| Journal Section | Articles |
| Authors | |
| Project Number | 12-YL-20 |
| Publication Date | December 31, 2023 |
| Published in Issue | Year 2023 Volume: 6 Issue: 2 |
