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The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum

Year 2022, Volume: 15 Issue: 2, 65 - 69, 31.12.2022
https://doi.org/10.47027/duvetfd.1140857

Abstract

This study is aimed to evaluate the relationship between VEGF and TLR4 expression in the ileum during broiler post-hatching development. The material for the study was taken from the ileum tissue of 7-, 21-, and 42-day-old broilers. In tissue sections VEGF and TLR4 expression were demonstrated by Streptavidin-biotin complex immunohistochemistry method. Beginning on the 7th day after hatching, the number of stained cells and staining intensity in the epithelial cells lining the villus intestinalis increased in TLR4 immunostaining. On the 7th day following hatching, TLR4 protein expression was not seen in crypt epithelial cells. At day 21, crypt epithelial cells began to stain and gave a more intense immunoreaction at day 42. In VEGF-stained sections, the ileum villus epithelial cells, crypt, and smooth muscle tissue showed a brown intracytoplasmic response. The expression of the VEGF protein in the upper villus epithelial cells started to increase on the 7th day, and it stained intensely, especially on the 42nd day. In addition, it was observed that the staining intensity of the tunica muscularis layer was the same on the 7th and 21st days, and increased on the 42nd day. It was remarkable that goblet cells gave negative results in both immunostaining. In summary it seen that TLR4 and VEGF expression were found to be increase in this study from the 7th to the 42nd day following hatching. Thus, it was concluded that angiogenesis mechanisms and the development of innate and adaptive defense systems continue throughout the post-hatching period.

References

  • 1. Dabravolski SA, Khotina VA, Omelchenko AV, Kalmykov VA, Orekhov AN. (2022). The role of the VEGF family in atherosclerosis development and ıts potential as treatment targets. Int J Mol Sci. 23(2): 931. 2. El-Zayat SR, Sibaii H, Mannaa FA. (2019). Toll-like receptors activation, signaling, and targeting: an overview. Bull Natl Res Cent. 43(187): 1-12.

Broiler İleumunda Kuluçka Sonrası Farklı Dönemlerin TLR4 ve VEGF Ekspresyon Paternleri Üzerine Etkisi

Year 2022, Volume: 15 Issue: 2, 65 - 69, 31.12.2022
https://doi.org/10.47027/duvetfd.1140857

Abstract

Bu çalışmanın amacı, etlik piliçlerin kuluçka sonrası gelişimi sırasında ileumda VEGF ve TLR4 ekspresyonu arasındaki ilişkiyi değerlendirmektir. Çalışma materyali 7-, 21- ve 42 günlük piliçlerin ileum dokusundan alındı. Doku kesitlerinde VEGF ve TLR4 ekspresyonu, Streptavidin-biotin kompleksi immünohistokimya yöntemi ile gösterildi. Kuluçkadan sonraki yedinci günden başlayarak, TLR4 immün boyamasında villus intestinalisi kaplayan epitel hücrelerinde boyanan hücre sayısı ve boyama yoğunluğu arttı. Yumurtadan çıkmayı takip eden 7. günde, kript epitel hücrelerinde TLR4 protein ekspresyonu yoktu. 21. günde kript epitel hücreleri lekelenmeye başladı ve 42. günde daha yoğun bir immünreaksiyon verdi. VEGF ile boyanmış bölümlerde, ileum villus epitel hücreleri, kript ve düz kas dokusu kahverengi bir intrasitoplazmik tepki gösterdi. VEGF proteininin üst villus epitel hücrelerinde ekspresyonu 7. günde artmaya başladı ve özellikle 42. günde yoğun boyandı. Ayrıca tunika muskularis tabakasının boyanma yoğunluğunun 7. ve 21. günlerde aynı olduğu, 42. günde arttığı gözlendi. Goblet hücrelerinin her iki immün boyamada da negatif sonuç vermesi dikkat çekiciydi. Özetle bu çalışmada kuluçkadan sonraki 7. günden 42. güne kadar TLR4 ve VEGF ekspresyonunun arttığı görülmüştür. Böylece anjiyogenez mekanizmalarının ve doğuştan gelen ve adaptif savunma sistemlerinin gelişiminin kuluçka sonrası dönem boyunca devam ettiği sonucuna varılmıştır.

References

  • 1. Dabravolski SA, Khotina VA, Omelchenko AV, Kalmykov VA, Orekhov AN. (2022). The role of the VEGF family in atherosclerosis development and ıts potential as treatment targets. Int J Mol Sci. 23(2): 931. 2. El-Zayat SR, Sibaii H, Mannaa FA. (2019). Toll-like receptors activation, signaling, and targeting: an overview. Bull Natl Res Cent. 43(187): 1-12.
There are 1 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Tuğrul Ertuğrul 0000-0002-9310-1200

Ali Çalık 0000-0003-4550-9321

Ahmet Ceylan 0000-0001-5878-8775

Şerife Tütüncü 0000-0001-6834-7244

Publication Date December 31, 2022
Acceptance Date September 9, 2022
Published in Issue Year 2022 Volume: 15 Issue: 2

Cite

APA Ertuğrul, T., Çalık, A., Ceylan, A., Tütüncü, Ş. (2022). The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 15(2), 65-69. https://doi.org/10.47027/duvetfd.1140857
AMA Ertuğrul T, Çalık A, Ceylan A, Tütüncü Ş. The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum. Dicle Üniv Vet Fak Derg. December 2022;15(2):65-69. doi:10.47027/duvetfd.1140857
Chicago Ertuğrul, Tuğrul, Ali Çalık, Ahmet Ceylan, and Şerife Tütüncü. “The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 15, no. 2 (December 2022): 65-69. https://doi.org/10.47027/duvetfd.1140857.
EndNote Ertuğrul T, Çalık A, Ceylan A, Tütüncü Ş (December 1, 2022) The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum. Dicle Üniversitesi Veteriner Fakültesi Dergisi 15 2 65–69.
IEEE T. Ertuğrul, A. Çalık, A. Ceylan, and Ş. Tütüncü, “The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum”, Dicle Üniv Vet Fak Derg, vol. 15, no. 2, pp. 65–69, 2022, doi: 10.47027/duvetfd.1140857.
ISNAD Ertuğrul, Tuğrul et al. “The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 15/2 (December 2022), 65-69. https://doi.org/10.47027/duvetfd.1140857.
JAMA Ertuğrul T, Çalık A, Ceylan A, Tütüncü Ş. The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum. Dicle Üniv Vet Fak Derg. 2022;15:65–69.
MLA Ertuğrul, Tuğrul et al. “The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 15, no. 2, 2022, pp. 65-69, doi:10.47027/duvetfd.1140857.
Vancouver Ertuğrul T, Çalık A, Ceylan A, Tütüncü Ş. The Effect of Different Post-Hatching Periods on TLR4 and VEGF Expression Patterns in Broiler Ileum. Dicle Üniv Vet Fak Derg. 2022;15(2):65-9.