Akut Gastroenteritli Çocuklarda Grup A Rota Virus Antijen Sıklığının İmmünokromatik Yöntemle Araştırılması
Abstract
Rotavirüs dünyadaki çocuk ölümlerinin önemli bir faktörüdür ve çocukluk çağında görülen akut gastroenteritin en büyük sebebidir. Rotavirüs enfeksiyonları yatış gerektiren ağır enfeksiyonlar şeklinde görüldüğü gibi hafif enfeksiyon şeklinde de seyredebilir. Mortalite ve morbidite yanında bir takım ekonomik kayıplarada sebeb olabilir. Bu çalışma çocuklarda görülen rotavirüs gastroenteritinin bölgemizdeki epidemiyolojisinin tespiti amacıyla yapılmıştır. Akut gastoenterit şikayeti ile acil kliniğine başvuran 0-14 yaş arası çocuklardan elde edilen dışkı örnekleri rotavirüs açısından değerlendirildi. Üretici firmanın talimatlarına göre Rota virüs A antijeni immünokramatografik yöntemle incelendi. 3102 örnekten 318’inde viral antijenler saptandı (%10.2). Hastaların 179’u (%56.3) erkek, 139’u (%43.7) kadındı. Rotavirüs antijeni en yüksek olarak 2 yaşından küçüklerde gözlemlendi (%44.3; n = 141). Pozitif oran özellikle Ocak, Şubat, Mart ta en yüksek düzeyine ulaşmıştır. Özellikle kış aylarında görülen gastroenteritlerde Rota virüs önemli bir faktör olarak karşımıza çıkmaktadır. Gastroenteritin en büyük sebebi olan rota virüs, dışkıdaki serogrup A antijeninden saptanabilir. İmmünokramatografik yöntemle antijeni hızlı bir şekilde belirlemek mümkündür. Çok kısa sürede dışkıdan antijenin belirleyen ve ishali teşhis eden immünokramatografik yöntemler son derece önemlidir. Gasroenteritli çocuklarda viral faktörlerin hızlı bir şekilde teşhis edilmesinin gereksiz antibiyotik kullanımının önüne geçeceğine inanıyoruz.
References
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- 2. Cook SM, Glass RI, LeBaron CW, Ho MS. Gobal seasonality of rotavirüs infecti¬ons. Bull World Healt Organ 1990; 68: 171-7.
- 3. Gray J, Vesikari T, Van Damme P, Giaquinto C, Mrukowicz J, Guarino A, Dagan R, Szajewska H, Usonis V. Rotavirus. J Pediatr Gastroenterol Nu, 2008;46:S24-31.
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- 15.Vesikari T, Rautanen T, Von Bonsdorff CH: Rotavirus gastroenteritis in Finland: burden of disease and epidemiological features, Acta Paediatr Suppl 1999;88(426):24-30.
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Investigation of Group A Rotavirus Antigen Frequency in Children Who Have Acute Gastroenteritis with Immunochromatographic Methods
Abstract
Rotavirus is the major
cause of acute gastroenteritis in infants and young children worldwide and is
the important agent of child deaths. Rotavirus infections represent in
different clinical pictures ranging between mild diarrhea to serious cases
requiring hospitalization. In addition to causing morbidity and mortality in
children, rotavirus gastroenteritis creates a major economic burden on health
care systems and families. To investigate the epidemiology of rotavirus
infections in children with acute gastroenteritis in our region. Feces samples
of 0- to 14-year-old children who were admitted to emergency department with
acute gastroenteritis were examined in terms of rotavirus A antigen with the
immunochromatographic method, using the manufacturer’s instructions. Viral
antigens were detected in 318 of 3102 samples (10.2%). Of the patients, 179
(56.3%) were male and 139 (43.7%) female. The highest rate of rotavirus antigen
positivity was observed in patients younger than 2 years (44.3%; n = 141).
Positivity rate peaked in the January-February-March, especially in winter
months, rotavirus is an important agent in children with acute gastroenteritis.
Diarrhea due to rotavirus, the most common cause, can be detected from
serogroup A antigen in the feces. It is possible to detect with the
immunochromatographic method, which is a rapid testing method.
immunochromatographic methods that can detect antigens from feces and diagnose
diarrhea in a short time period is important. We believe that when patients
present with diarrhea, diagnosed the viral factor accurately and quickly, which
could also prevent unnecessary use of antibiotics.
References
- 1. Kosek M, Bern C, Guerrant RL. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ 2003; 81: 197-204.
- 2. Cook SM, Glass RI, LeBaron CW, Ho MS. Gobal seasonality of rotavirüs infecti¬ons. Bull World Healt Organ 1990; 68: 171-7.
- 3. Gray J, Vesikari T, Van Damme P, Giaquinto C, Mrukowicz J, Guarino A, Dagan R, Szajewska H, Usonis V. Rotavirus. J Pediatr Gastroenterol Nu, 2008;46:S24-31.
- 4. Saronio-Gabarro MS, Mrukowicz J, Vesikari T, Verstraeten T. Burden of rotavirus disease in Eu-ropean Union countries, Pediatr Infect Dis J 2006;25(Suppl 1):7-11.
- 5. Glass RI, Kilgore PE, Holman RC et al: The epide-miology of rotavirus diarrhoea in the United Sta-tes: surveillance and of disease burden, J Infect Dis 1996;174(Suppl 1):5-11
- 6. Ellen S, Dante A, Sharon, Humiston, Dante A, Sharon G. Rotavirus. Pediatrics in Review, 2007; 8 No.5.
- 7. Ramsay M, Brown D. Epidemiology of group A rotaviruses. In: Gray J, Desselberger U, eds. Rotaviruses: Methods and Protocols. Totowa, NJ: Humana Press Inc., 2000: 217-36.
- 8. Sénécal M, Brisson M, Lebel MH, et al. Measuring the impact of rotavirus acute gastroenteritis episodes (MIRAGE): A prospective community-based study. Can J Infect Dis Med Microbiol. 2008; 19(6): 397-404.
- 9. Global networks for surveillance of rotavirus gastroenteritis, 2001-2008. Wkly Epidemiol Rec. 2008; 83(47): 421-5.
- 10. Regagnon C, Chambon M, Archimbaud C, et al. Rapid diagno-sis of rotavirus infections: comparative prospective study of two techniques for antigen detection in stool. Pathol Biol (Paris). 2006; 54(6): 343-6
- 11. Lee SY, Hong JH, Lee SW, Lee M. Comparisons of latex agglutination, immunochromatography and enzyme immunoassay methods for the detection of rotavirus antigen. Korean J Lab Med 2007; 27: 437-441.
- 12. Fischer TK: Incidence of hospitalizations due to rotavirus gastroenteritis in Denmark, Acta Paediatr 2001;90(9):1073-5.
- 13. Gil A, Carrasco P, Jimenez R, San-Martin M, Oyaguez I, Gonzalez A: Burden of hospitalizations attributable to rotavirus infection in children in Spain, period 1999-2000, Vaccine 2004;22(17-18):2221-5.
- 14.Nakagomi T, Nakagomi O, Takahashi Y, Enoki M, Suzuki T, Kilgore PE: Incidence and burden of rotavirus gastroenteritis in Japan, as estimated from a prospective sentinel hospital study, J Infect Dis 2005;192(Suppl 1):S106-10.
- 15.Vesikari T, Rautanen T, Von Bonsdorff CH: Rotavirus gastroenteritis in Finland: burden of disease and epidemiological features, Acta Paediatr Suppl 1999;88(426):24-30.
- 16. Akıncı N, Ercan TE, Yalman N, Eren A, Severge B, Ercan G. Akut gastroenteritli çocuklarda adenovirüs ve rotavirüs. Çocuk Enfeks Derg. 2007; 1: 98-101.
- 17. Altındiş M, Yavru S, Şimşek A, Özkul A, Çeri A, Koç H. Rotavirus infection in children with acute diarrhea as detected by latex agglutination, ELISA and poliacrylamide gel electrophoresis. Indian Pediatr. 2004; 41(6): 590-4.
- 18. Kurugöl Z, Geylani S, Karaca Y, et al. Rotavirus gastroenteritis among children under fi ve years of age in İzmir, Turkey. Turk J Pediatr. 2003; 45(4): 290-4.
- 19. William CJ, Gray J, Pebody RG, Lobanov A. Survey of rotavirus surveillance, laboratory capacity and disease burden in eastern part of the WHO European Region. Euro Surveill. 2008; 13(34): pii: 18959.
- 20. Ramsay M, Brown D. Epidemiology of group A rotaviruses. In: Gray J, Desselberger U, eds. Rotaviruses: Methods and Protocols. Totowa, NJ: Humana Press Inc.2000: 217-36.
- 21. Rosenfeldt V, Vesikari T, Pang XL, Zeng SQ, Tvede M, Paerregaard A. Viral etiology and incidence of acute gastroenteritis in young children attending day-care centers. Pediatr Infect Dis J. 2005; 24(11): 962-5.
- 22. Gül M, Garipardıç M, Çıragil P, Aral M, Karabiber H, Güler Y. 0-5 Yaş Arası Gastroenteritli Çocuklarda Rotavirüs ve Adenovirüs Tip 40/41 Araştırılması. ANKEM Derg 2005; 19: 64-7.
- 23. Koopmans M, Brown D. Seasonality and diversity of group A rotaviruses in Europe. Acta Pediatr Suppl. 1999; 88(426): 14-9.
Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | September 24, 2017 |
| Submission Date | November 2, 2016 |
| Published in Issue | Year 2017 Volume: 7 Issue: 2 |
