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Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze

Year 2020, Volume: 3 Issue: 2, 106 - 109, 29.12.2020

Merve Şifa Hane Köse Muhammet Doğan Gökhan Sadi

https://doi.org/10.46239/ejbcs.728152

Abstract

Staurogyne repens (Nees) Kuntze is an aquatic plant belonging to Acanthaceae family. It is a valuable plant in the aquarium industry. Therefore, there has been a significant demand for this plant. This study was designed for multiple and efficient productions of S. repens under in vitro conditions. He used nodal meristems as the type of explant. Nodal meristems were preferred as explant type. The explants were transferred to Murashige and Skoog (MS) food media with TDZ single (0-1.50 mg/L) and TDZ (0.25-1.50 mg/L) + IAA (0.25 mg/L) combinations. In TDZ application, the most number of shoots per explant (15.36 shoots/explants) was recorded in cultures with 1.50 mg/L TDZ, while in TDZ + IAA application, the most number of shoots (9.44 shoots/explants) was determined in culture with 1.0 mg/L TDZ + 0.25 mg/L IAA. In general, shoot lengths in TDZ + IAA combination was measured higher than the single application of TDZ. The longest shoot (1.67 cm) was obtained in the culture medium with 1.0 mg/L TDZ + 0.25 mg/L IAA. The extended shoots were transposed to rooting media with 0.25 mg/L IAA, and multiple root formations were determined after four weeks. The rooted plants were transferred to the aquarium and successfully accustomed to ex vitro conditions.

Keywords

Acclimatization propagation shoot regeneration tissue culture

References

  • Aasim M, Karatas M, Khawar KM, Dogan M. 2013. Optimization of sterilization and micropropagation of water lettuce (Pistia stratiotes L.). J Appl Biol Sci. 7(3):71-74.
  • Akaracharanya A, Choi YE, Kusano T, Shinmyo A, Sano H. 2001. Efficient plant regeneration of ıpomoea aquatica by direct shoot formation from cotyledon segments. Plant Biotechnol. 18(1):77-79.
  • Altman A. 2019. Plant tissue culture and biotechnology: perspectives in the history and prospects of the International Association of Plant Biotechnology (IAPB). In Vitro Cell Dev Biol Plant. 55(5):590-594.
  • Arigundam U, Variyath AM, Siow YL, Marshall D, Debnath SC. 2020. Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors. Sci Hortic. 264:109199.
  • Cheruvathur MK, Abraham J, Mani B, Thomas TD. 2010. Adventitious shoot induction from cultured internodal explants of Malaxis acuminata D. Don, a valuable terrestrial medicinal orchid. Plant Cell Tiss Org. 101:163-170.
  • Dewir YH, Naidoo Y, da Silva, JAT 2018. Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Rep. 37(11):1451-1470.
  • Dogan M. 2018. In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech. 8:77-84.
  • Dogan M. 2019. Multiple shoot regeneration via ındirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. J Anim Plant Sci, 29(2):568-577.
  • Efferth T. 2019. Biotechnology applications of plant callus cultures. Engineering, 5(1):50-59.
  • El-Sherif NA. 2018. Impact of plant tissue culture on agricultural sustainability. In Sustainability of Agricultural Environment in Egypt: Part II, Springer, Cham. pp. 93-107.
  • Gaikwad AV, Singh SK, Gilhotra R. 2017. Plant tissue culture-A review. Indian J Pharm Educ. 2:217-220.
  • Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev Biol Plant. 32(4):272-289.
  • Gehl C, Li G, Serek M. 2020. An efficient protocol for Agrobacterium-mediated transformation and regeneration of Campanula medium (Canterbury bells) based on leaf disc explants. Plant Cell Tiss Org. 140(3):635-645.
  • Hasancebi S, Kara NT, Çakır Ö, Arı Ş. 2011. Micropropagation and root culture of turkish endemic Astragalus chrysochlorus (Leguminosae). Turk J Bot. 35(2):203-210.
  • Hesami. M, Daneshvar MH. 2018. In vitro adventitious shoot regeneration through direct and indirect organogenesis from seedling-derived hypocotyl segments of Ficus religiosa L.: an important medicinal plant. HortScience. 53(1):55-61.
  • Kapruwan S, Bakshi M, Kaur M. 2014. Rapid in vitro propagation of the solid bamboo, dendrocalamus strictusnees, through axillary shoot proliferation. Biotechnol Int. 7(3):58-68.
  • Kher MM, Joshi D, Nekkala S, Nataraj M, Raykundaliya DP. 2014. Micropropagation of Pluchea lanceolata (Oliver & Hiern.) using nodal explant. Hortic Res. 22(1):35-39.
  • Kumar Mishra A, Nath Tiwari K, Mishra P, Kumar Tiwari S, Kumar Mishra S, Singh J. 2020. Factors affecting the efficiency of in vitro regeneration from seedling-derived nodal explants of Nyctanthes arbor-tristis L. and evaluation of genetic fidelity. Plant Biosyst. 154(2):197-205.
  • Luan VQ, Tung HT, Hien VT, Hieu T, Nhut DT. 2019. Effects of shoot tip removal, wounding manipulation, and plant growth regulators on shoot regeneration and plantlet development in Paphiopedilum species. Sci Hortic. 256:108648.
  • Mahna N, Vahed SZ, Hani S. 2013. Plant in vitro culture goes nano: nanosilver-mediated decontamination of ex vitro explants. Nanomed-Nanotechnol. 4(161):1.
  • Matand K, Shoemake M, Li C. 2020. High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron. BMC Plant Biol. 20(1):31.
  • Mineo L. 1990. Plant tissue culture techniques. Tested Studies For Laboratory Teaching, 11:151-174.
  • Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15(3):473-497.
  • Pandey S, Pandey VC. 2018. Plant tissue culture techniques and its achievements. In Advanced Molecular Plant Breeding: Meeting the Challenge of Food Security, CRC Press.
  • Phillips GC, Garda, M. 2019. Plant tissue culture media and practices: an overview. In Vitro Cell Dev Biol Plant. 55(3):242-257.
  • Praveen N, Naik PM, Manohar SH, Nayeem A. Murthy, HN. 2009. In Vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant. 31(4):723-728.
  • Siddique I, Anis M, Aref I. M. 2010. In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl.—a potential medicinal plant. Appl Biochem Biotechnol. 162(7):2067-2074.
  • Siwach P. Gill AR. 2011. Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine and phloroglucinol. Physiol Mol Biol Pla, 17(3):271.
  • Snedecor GW, Cochran WG. 1967. Statistical methods. The Iowa State Univ Press, pp 327-329.
  • Wang J, Seliskar DM, Gallagher JL. 2003. Tissue culture and plant regeneration of Spartina alterniflora: implications for wetland restoration. Wetlands. 23(2):386-393.

Year 2020, Volume: 3 Issue: 2, 106 - 109, 29.12.2020

Merve Şifa Hane Köse Muhammet Doğan Gökhan Sadi

https://doi.org/10.46239/ejbcs.728152

Abstract

References

  • Aasim M, Karatas M, Khawar KM, Dogan M. 2013. Optimization of sterilization and micropropagation of water lettuce (Pistia stratiotes L.). J Appl Biol Sci. 7(3):71-74.
  • Akaracharanya A, Choi YE, Kusano T, Shinmyo A, Sano H. 2001. Efficient plant regeneration of ıpomoea aquatica by direct shoot formation from cotyledon segments. Plant Biotechnol. 18(1):77-79.
  • Altman A. 2019. Plant tissue culture and biotechnology: perspectives in the history and prospects of the International Association of Plant Biotechnology (IAPB). In Vitro Cell Dev Biol Plant. 55(5):590-594.
  • Arigundam U, Variyath AM, Siow YL, Marshall D, Debnath SC. 2020. Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors. Sci Hortic. 264:109199.
  • Cheruvathur MK, Abraham J, Mani B, Thomas TD. 2010. Adventitious shoot induction from cultured internodal explants of Malaxis acuminata D. Don, a valuable terrestrial medicinal orchid. Plant Cell Tiss Org. 101:163-170.
  • Dewir YH, Naidoo Y, da Silva, JAT 2018. Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Rep. 37(11):1451-1470.
  • Dogan M. 2018. In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech. 8:77-84.
  • Dogan M. 2019. Multiple shoot regeneration via ındirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. J Anim Plant Sci, 29(2):568-577.
  • Efferth T. 2019. Biotechnology applications of plant callus cultures. Engineering, 5(1):50-59.
  • El-Sherif NA. 2018. Impact of plant tissue culture on agricultural sustainability. In Sustainability of Agricultural Environment in Egypt: Part II, Springer, Cham. pp. 93-107.
  • Gaikwad AV, Singh SK, Gilhotra R. 2017. Plant tissue culture-A review. Indian J Pharm Educ. 2:217-220.
  • Gaspar T, Kevers C, Penel C, Greppin H, Reid DM, Thorpe TA 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev Biol Plant. 32(4):272-289.
  • Gehl C, Li G, Serek M. 2020. An efficient protocol for Agrobacterium-mediated transformation and regeneration of Campanula medium (Canterbury bells) based on leaf disc explants. Plant Cell Tiss Org. 140(3):635-645.
  • Hasancebi S, Kara NT, Çakır Ö, Arı Ş. 2011. Micropropagation and root culture of turkish endemic Astragalus chrysochlorus (Leguminosae). Turk J Bot. 35(2):203-210.
  • Hesami. M, Daneshvar MH. 2018. In vitro adventitious shoot regeneration through direct and indirect organogenesis from seedling-derived hypocotyl segments of Ficus religiosa L.: an important medicinal plant. HortScience. 53(1):55-61.
  • Kapruwan S, Bakshi M, Kaur M. 2014. Rapid in vitro propagation of the solid bamboo, dendrocalamus strictusnees, through axillary shoot proliferation. Biotechnol Int. 7(3):58-68.
  • Kher MM, Joshi D, Nekkala S, Nataraj M, Raykundaliya DP. 2014. Micropropagation of Pluchea lanceolata (Oliver & Hiern.) using nodal explant. Hortic Res. 22(1):35-39.
  • Kumar Mishra A, Nath Tiwari K, Mishra P, Kumar Tiwari S, Kumar Mishra S, Singh J. 2020. Factors affecting the efficiency of in vitro regeneration from seedling-derived nodal explants of Nyctanthes arbor-tristis L. and evaluation of genetic fidelity. Plant Biosyst. 154(2):197-205.
  • Luan VQ, Tung HT, Hien VT, Hieu T, Nhut DT. 2019. Effects of shoot tip removal, wounding manipulation, and plant growth regulators on shoot regeneration and plantlet development in Paphiopedilum species. Sci Hortic. 256:108648.
  • Mahna N, Vahed SZ, Hani S. 2013. Plant in vitro culture goes nano: nanosilver-mediated decontamination of ex vitro explants. Nanomed-Nanotechnol. 4(161):1.
  • Matand K, Shoemake M, Li C. 2020. High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron. BMC Plant Biol. 20(1):31.
  • Mineo L. 1990. Plant tissue culture techniques. Tested Studies For Laboratory Teaching, 11:151-174.
  • Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15(3):473-497.
  • Pandey S, Pandey VC. 2018. Plant tissue culture techniques and its achievements. In Advanced Molecular Plant Breeding: Meeting the Challenge of Food Security, CRC Press.
  • Phillips GC, Garda, M. 2019. Plant tissue culture media and practices: an overview. In Vitro Cell Dev Biol Plant. 55(3):242-257.
  • Praveen N, Naik PM, Manohar SH, Nayeem A. Murthy, HN. 2009. In Vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant. 31(4):723-728.
  • Siddique I, Anis M, Aref I. M. 2010. In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl.—a potential medicinal plant. Appl Biochem Biotechnol. 162(7):2067-2074.
  • Siwach P. Gill AR. 2011. Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine and phloroglucinol. Physiol Mol Biol Pla, 17(3):271.
  • Snedecor GW, Cochran WG. 1967. Statistical methods. The Iowa State Univ Press, pp 327-329.
  • Wang J, Seliskar DM, Gallagher JL. 2003. Tissue culture and plant regeneration of Spartina alterniflora: implications for wetland restoration. Wetlands. 23(2):386-393.
There are 30 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Merve Şifa Hane Köse

Muhammet Doğan

Gökhan Sadi

Publication Date December 29, 2020
Acceptance Date November 19, 2020
Published in Issue Year 2020 Volume: 3 Issue: 2

Cite

APA Köse, M. Ş. H., Doğan, M., & Sadi, G. (2020). Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze. Eurasian Journal of Biological and Chemical Sciences, 3(2), 106-109. https://doi.org/10.46239/ejbcs.728152
AMA Köse MŞH, Doğan M, Sadi G. Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze. Eurasian J. Bio. Chem. Sci. December 2020;3(2):106-109. doi:10.46239/ejbcs.728152
Chicago Köse, Merve Şifa Hane, Muhammet Doğan, and Gökhan Sadi. “Multiple Shoot Induction and Plant Regeneration of Staurogyne Repens (Nees) Kuntze”. Eurasian Journal of Biological and Chemical Sciences 3, no. 2 (December 2020): 106-9. https://doi.org/10.46239/ejbcs.728152.
EndNote Köse MŞH, Doğan M, Sadi G (December 1, 2020) Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze. Eurasian Journal of Biological and Chemical Sciences 3 2 106–109.
IEEE M. Ş. H. Köse, M. Doğan, and G. Sadi, “Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze”, Eurasian J. Bio. Chem. Sci., vol. 3, no. 2, pp. 106–109, 2020, doi: 10.46239/ejbcs.728152.
ISNAD Köse, Merve Şifa Hane et al. “Multiple Shoot Induction and Plant Regeneration of Staurogyne Repens (Nees) Kuntze”. Eurasian Journal of Biological and Chemical Sciences 3/2 (December 2020), 106-109. https://doi.org/10.46239/ejbcs.728152.
JAMA Köse MŞH, Doğan M, Sadi G. Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze. Eurasian J. Bio. Chem. Sci. 2020;3:106–109.
MLA Köse, Merve Şifa Hane et al. “Multiple Shoot Induction and Plant Regeneration of Staurogyne Repens (Nees) Kuntze”. Eurasian Journal of Biological and Chemical Sciences, vol. 3, no. 2, 2020, pp. 106-9, doi:10.46239/ejbcs.728152.
Vancouver Köse MŞH, Doğan M, Sadi G. Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze. Eurasian J. Bio. Chem. Sci. 2020;3(2):106-9.
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