Research Article
Year 2022,
Volume: 5 Issue: 2, 95 - 99, 28.12.2022
Abstract
Investigation various nitrogen sources effects on the production of invertase by Aspergillus niger was researched in this study. Invertase is a precious enzyme used in many industries like food, pharmacy, confectionery, invert syrup production. Taguchi design of experiment (DOE) was preferred to optimize the cultivation conditions. L16 (43) orthogonal array was selected in the current study including nitrogen source, initial pH of the medium and incubation time at four levels for statistical optimization. The data showed that optimized version of invertase production was achieved using proteose peptone, 5.5 initial pH and 3 days for incubation time. Bacto peptone had higher enzyme activity than casein and yeast extract. pH of the medium was found as the most efficient factor among nitrogen source and incubation time. Besides, percentage contribution of the nitrogen source and incubation time were indicated at similar rates (9 and 10%, respectively). The highest enzyme activity was defined as 45.87 U/ml, which was found to be closer to the predicted result (46.33 U/ml). As a conclusion, proteose peptone increased the invertase activity and use of Taguchi DOE supported quick and effective optimization.
References
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Year 2022,
Volume: 5 Issue: 2, 95 - 99, 28.12.2022
Abstract
References
- Abarca ML, Accensi F, Cano J, Cabanes F J. 2004. Taxonomy and significance of black aspergilli. Antonie van Leeuwenhoek. 86(1), 33–49.
- Abdel-Rahman, MA, Hassan SE, El-Din MN, Azab M S, El-Belely EF, Alrefaey HMA, Elsakhawy T. 2020. One-factor-at-a-time and response surface statistical designs for improved lactic acid production from beet molasses by Enterococcus hirae ds10. SN Appl Sci. 2(4), 1–14.
- Aranda C, Robledo A, Loera O, Contreas-Esquivel JC, Rodriguez, R, Aguilar CN. 2006. Fungal Invertase Expression in Solid-State Fermentation. Food Tech Biotech 44(2), 229–233.
- Ashokkumar B, Kayalvizhi N, Gunasakaran P. 2001. Optimization of media for B-fructofuranosidase production by Aspergillus niger in submerged and solid state fermentation. Process Biochem. 37, 331–338.
- Boddy LM, Berges T, Barreau C, Vainstein MH, Donbson MJ, Ballance DJ, Pederdy JF 1993. Purification and characterization of an Aspergillus niger invertase and its DNA sequence. Curr Genet. 24, 60–66.
- Canli O, Erdal S, Taskin M, Kurbanoglu EB 2011. Effects of extremely low magnetic field on the production of invertase by Rhodotorula glutinis. Toxicol Ind Health. 27(1), 35–39. Das D, Bose A, Chattopadhyay P, Ray L. 2016. Production and Characterization of a Crude Thermostable Invertase from Cryptococcus laurentii S23. Indian Chem Eng. 58(2), 118–135.
- Farid MA, Ghoneimy EA, El-Khawaga MA, Negm-Eldein A Awad GEA. 2013. Statistical optimization of glucose oxidase production from Aspergillus niger NRC9 under submerged fermentation using response surface methodology. Ann Microbiol. 63(2), 523–531.
- Ge XY, Zhang WG. 2005. Effects of octadecanoylsucrose derivatives on the production of inulinase by Apergillus niger SL-09. World J Microbiol Biotechnol 21(8–9), 1633–1638.
- Ikram-Ul-Haq, Baig MA, Ali S. 2005. Effect of cultivation conditions on invertase production by hyperproducing Saccharomyces cerevisiae isolates. World J Microbiol Biotechnol. 21(4), 487–492.
- Jean MD, Tzeng YF. 2003. Use of Tacuchi methods and multiple regression analysis for optimal process development of high energy electron beam case hardening of cast iron. Surface Eng. 19(2), 150–156.
- Kotwal SM, Shankar V. 2009. Immobilized invertase. Biotechnol Adv. 27, 311–322.
- Laothanachareon T, Bunterngsook B, Champreda V. 2022. Profiling multi-enzyme activities of Aspergillus niger strains growing on various agro-industrial residues. 3 Biotech, 12(1), 1–16.
- Li C, Zhou J, Du G, Chen J, Takahashi S. 2020. Developing Aspergillus niger as a cell factory for food enzyme production. Biotechnol Adv. 44(107630).
- Miller GL. 1959. Use of dinitro salicylic acid reagent for determination of reducing sugar. Analytical Chem. 31, 426–428.
- Nadeem H, Rashid M H, Siddique MH, Azeem F, Muzammil S, Javed MR., Riaz M. 2015. Microbial invertases: A review on kinetics, thermodynamics, physiochemical properties. Process Biochem. 50(8), 1202–1210.
- Oyedeji O, Bakare MK, Adewale IO, Olutiola PO, Omoboye OO. 2017. Optimized production and characterization of thermostable invertase from Aspergillus niger IBK1, using pineapple peel as alternate substrate. Biocat Agri Biotechnol. 9(July 2016), 218–223.
- Rao RS, Kumar CG, Prakasham RS, Hobbs PJ. 2008. The Taguchi methodology as a statistical tool for biotechnological applications: A critical appraisal. Biotechnol J. 3(4), 510–523.
- Rubio MC, Navaroo AR. 2006. Regulation of invertase synthesis in Aspergillus niger. Enzyme Microb Technol. 39, 601–606.
- Rubio MC, Runco R, Navarro AR. 2002. Invertase from a strain of Rhodotorula glutinis. Phytochem. 61, 605–609.
- Shafiq K, Ali S, Haq I 2001. Effect of Different Mineral Nutrients on Invertase Production by Saccharomyces cerevisiae GCB-K5. Biotechnol (Faisalabad). 1 (1) 40-44.
- Tan O, Zaimoglu AS, Hinislioglu S, Altun S. 2005. Taguchi approach for optimization of the bleeding on cement-based grouts. Tunnel Underground Space Technol 20(2), 167–173.
- Tasar OC. 2017. Enhanced β-fructofuranosidase biosynthesis by Rhodotorula glutinis using Taguchi robust design method. Biocat Biotransformation 35(3), 191–196.
- Tasar OC. 2022. Glucose oxidase production using a medicinal plant: Inula viscosa and optimization with Taguchi DOE . J Food Process Preserv. 46:e16375.
- Tasar ÖC. 2020. Inulinase production capability of a promising medicinal plant: Inula viscosa. Commagene J Biol. 4, 67–73.
- Taskin M, Esim N, Genisel M, Ortucu S, Hasenekoglu I, Canli O, Erdal S. 2013. Enhancement of invertase production by aspergillus niger OZ-3 using low-intensity static magnetic fields. Prep Biochem Biotechnol. 43(2), 177–188.
There are 25 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 28, 2022 |
| Acceptance Date | July 31, 2022 |
| Published in Issue | Year 2022 Volume: 5 Issue: 2 |
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