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Bazı Tıbbi Ve Aromatik Bitki Yağlarının Sterol Profili Üzerine Silyl Türevlendirmenin Etkisi

Year 2019, Issue: 17, 360 - 365, 31.12.2019
https://doi.org/10.31590/ejosat.597071

Abstract

Bu çalışmada, bazı tıbbi ve aromatik bitkilerin (çemen, rezene, kişniş, çörek otu ve anason) sterol kompozisyonu (brassicasterol, campesterol, stigmasterol, β-Sitosterol and β-sitostanol) belirlenmiş ve numune hazırlama işleminde kullanılan iki farklı yöntem karşılaştırılmıştır. İlk yöntemde silyl türevlendirme işlemi uygulanmış ve diğer yöntemde türevlendirme maddeleri kullanılmamıştır. Numunelerin ham yağ oranlarının çemen için % 4,27 - 4,63, rezene için % 8,60 - 9,10, kişniş için % 9,57 - 10,70, çörek otu için % 22,77 - 23,50 ve anason için % 11,10 - 11,50 arasında değiştiği tespit edilmiştir. Her iki yöntemde de, sitosterol, tüm yağlar için major sterol bileşiği olarak tespit edilmiştir. Türevlendirme maddeleri kullanılarak gerçekleştirilen yöntem için daha düşük sterol konsantrasyonlarının kaydedildiği görülmüştür. Brassicasterol, türevlendirme ajanları olmadan gerçekleştirilen yöntemde sadece anason ve rezene yağlarında tanımlanırken, diğer yöntemde sadece çörek otu yağında tespit edilmiştir. Literatüre göre, türevlendirme işleminin daha uyumlu olduğu sonucuna varılmıştır.

Supporting Institution

Erciyes Üniversitesi

Project Number

FHD-2017-7086

References

  • Alonso L., Fontecha J., Lozada L. and Juárez M. (1997). Determination of mixtures in vegetable oils and milk fat by analysis of sterol fraction by Gas Chromatography, Journal of American Oil Chemist’s Society, 74(2), 131-135.
  • Alvarez-Sala, A., Attanzio, A., Tesoriere, L., Garcia-Llatas, G., Barberá, R. and Cilla, A. (2018). Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines, International Journal of Food Sciences and Nutrition, 70(3), 323-334.
  • Awad A.B., and Fink, C.S. (2000). Phytosterols as anticancer dietary components: Evidence and mechanism of action, Journal of Nutrition, 130, 2127–2130.
  • Cheikh-Rouhou, S., Besbes, S., Hentati, B., Blecker, C., Deroanne, C., Attia, H. (2007). Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction, Food Chemistry, 101, 673–681.
  • Choudhary, S.P. and Tran, L.S. (2011). Phytosterols: perspectives in human nutrition and clinical therapy, Current Medicinal Chemistry, 18(29), 4557-4567.
  • Klaudija, C.S., Marko, P., Grdiša, M., Jasna, P., Dalibor, B., Mirjana, H.C., Zlatko, Š. (2016). Medicinal plants of the family Lamiaceaeas functional foods – A review, Czech Journal of Food Sciences, 34 (5), 377-390.
  • Gao, F., Wang, G., Wang, L. and Guo, N. (2017). Phytosterol nutritional supplement improves pregnancy and neonatal complications of gestational diabetes mellitus in a double-blind and placebo-controlled clinical study. Food Function, 8, 424-428.
  • Huang, J., Xu, M., Fang, Y., Lu, M., Pan, Z., Huang, W., Chem, Y.M. and Zhang, C. (2017). Association between phytosterol intake and colorectal cancer risk: A case–control study, British Journal of Nutrition, 117(6), 839-850.
  • Sriti, J., Talou, T., Msaada, K., and Marzouk, B. (2011). Comparative analysis of fatty acid, sterol and tocol composition of Tunisian and Canadian Coriander (Coriandrum sativum L.) fruit, Analytical Chemistry Letters, 1(5-6), 375-383.
  • Jones P.J.H. and AbuMweis S.S. (2009). Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer, Current Opinion in Clinical Nutrition and Metabolic Care, 12,147–151.
  • Kıralan, M., Yorulmaz, A., Çalıkoğlu, E. and Bayrak, A. (2017). Çemen otu (Trigonella foenum-graecum L.) tohumunun yağ asitleri ve sterol bileşimi, Derim 34(2),138-141.
  • Kozłowska, M., Gruczyńska, E., Ścibisz, I. and Rudzińska M. (2016). Fatty acids and sterols composition, and antioxidant activity of oils extracted from plant seeds, Food Chemistry, 213, 450-456.
  • Sujith Kumar, M.S., Mawlong, I. and Singh, D. (2017). Phytosterol recovery from oilseeds: Recent advances, Journal of Food Process Engineer, 40 (3), e12466.
  • Li, D., Park, J. and Oh, J. R. (2001). Silyl derivatization of alkylphenols, chlorophenols, and bisphenol A for simultaneous GC/MS determination, Analytical Chemistry, 73(13), 3089-3095.
  • Orem, A., Alasalvar, C., Kural, B. V., Yaman, S., Orem, C., Karadag, A., ... & Zawistowski, J. (2017). Cardio-protective effects of phytosterol-enriched functional black tea in mild hypercholesterolemia subjects, Journal of Functional Foods, 31, 311-319.
  • Piironen, V., Toivo, J. and Lampi, A.M. (2000). Natural sources of dietary plant sterols, Journal of Food Compositon and Analysis, 13, 619-624.
  • Poojary M.M. and Passamonti P. (2016). Improved conventional and microwave-assisted silylation protocols for simultaneous gas chromatographic determination of tocopherols and sterols: Method development and multi-response optimization, Journal of Chromatography A, 9, 88-104.
  • SAS (2000). SAS Institute Inc.: SAS Version 8.02: SAS/STAT Software: changes and enhancements through Release 8.02. Cary, NC, SAS Institute Inc, USA.
  • Rideout, T.C., Carrier, B., Wen, S., Raslawsky, A., Browne, R.W. and Harding, S.V. (2016). Complementary cholesterol-lowering response of a phytosterol/α-lipoic acid combination in obese Zucker rats, Journal of Dietary Supplements, 13, 283-299.
  • Uitterhaegen, E., Sampaio, K.A., Delbeke, E.I.P., De Greyt, W., Cerny, M., Evon, P., Merah, O., Talou, T. and Stevens, C.V. (2016). Characterization of French coriander oil as source of petroselinic acid, Molecules, 2, 1202.
  • Yang, R., Zhang, L., Li, P., Yu, L., Mao, J., Wang, X. and Zhang, Q. (2018). A review of chemical composition and nutritional properties of minor vegetable oils in China, Trends in Food Science & Technology, 74, 26-32,
  • Wojciechowski, Z. A. (1991) Biochemistry of phytosterol conjugates, in Physiology and Biochemistry of Sterols (Patterson, G.W. and Nes, W.D., eds), pp. 361–395, American Oil Chemists' Society.

Sterol Profile of Some Medicinal and Aromatic Plant Oils: Effect of Silyl Derivatization Process

Year 2019, Issue: 17, 360 - 365, 31.12.2019
https://doi.org/10.31590/ejosat.597071

Abstract

In this study, sterol composition (brassicasterol, campesterol, stigmasterol, β-Sitosterol and β-sitostanol) of the crude oils extracted from different parts (seed or fruit) of some medicinal and aromatic plants (fenugreek, fennel, coriander, black cumin and anise) was determined and two different methods used in sample preparation process were compared. In the first method, silyl derivatization process was applied and in the other method derivatizing agents were not used. It was determined that the oil levels of the samples ranged between 4.27 - 4.63% for fenugreek, 8.60 - 9.10% for fennel, 9.57 - 10.70% for coriander, 22.77 - 23.50% for black cumin and 11.10 - 11.50% for anise. In both methods, β- sitosterol was the major sterol compound for all oils. It was observed that lower sterol concentrations were recorded for the method performed using derivatizing agents. Brassicasterol was identified only in anise and fennel oils in the method performed without the derivatizing agents, while it was detected in only black cumin oil in the other method. According to literature, it was concluded that the derivatization process was more compatible.

Project Number

FHD-2017-7086

References

  • Alonso L., Fontecha J., Lozada L. and Juárez M. (1997). Determination of mixtures in vegetable oils and milk fat by analysis of sterol fraction by Gas Chromatography, Journal of American Oil Chemist’s Society, 74(2), 131-135.
  • Alvarez-Sala, A., Attanzio, A., Tesoriere, L., Garcia-Llatas, G., Barberá, R. and Cilla, A. (2018). Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines, International Journal of Food Sciences and Nutrition, 70(3), 323-334.
  • Awad A.B., and Fink, C.S. (2000). Phytosterols as anticancer dietary components: Evidence and mechanism of action, Journal of Nutrition, 130, 2127–2130.
  • Cheikh-Rouhou, S., Besbes, S., Hentati, B., Blecker, C., Deroanne, C., Attia, H. (2007). Nigella sativa L.: Chemical composition and physicochemical characteristics of lipid fraction, Food Chemistry, 101, 673–681.
  • Choudhary, S.P. and Tran, L.S. (2011). Phytosterols: perspectives in human nutrition and clinical therapy, Current Medicinal Chemistry, 18(29), 4557-4567.
  • Klaudija, C.S., Marko, P., Grdiša, M., Jasna, P., Dalibor, B., Mirjana, H.C., Zlatko, Š. (2016). Medicinal plants of the family Lamiaceaeas functional foods – A review, Czech Journal of Food Sciences, 34 (5), 377-390.
  • Gao, F., Wang, G., Wang, L. and Guo, N. (2017). Phytosterol nutritional supplement improves pregnancy and neonatal complications of gestational diabetes mellitus in a double-blind and placebo-controlled clinical study. Food Function, 8, 424-428.
  • Huang, J., Xu, M., Fang, Y., Lu, M., Pan, Z., Huang, W., Chem, Y.M. and Zhang, C. (2017). Association between phytosterol intake and colorectal cancer risk: A case–control study, British Journal of Nutrition, 117(6), 839-850.
  • Sriti, J., Talou, T., Msaada, K., and Marzouk, B. (2011). Comparative analysis of fatty acid, sterol and tocol composition of Tunisian and Canadian Coriander (Coriandrum sativum L.) fruit, Analytical Chemistry Letters, 1(5-6), 375-383.
  • Jones P.J.H. and AbuMweis S.S. (2009). Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer, Current Opinion in Clinical Nutrition and Metabolic Care, 12,147–151.
  • Kıralan, M., Yorulmaz, A., Çalıkoğlu, E. and Bayrak, A. (2017). Çemen otu (Trigonella foenum-graecum L.) tohumunun yağ asitleri ve sterol bileşimi, Derim 34(2),138-141.
  • Kozłowska, M., Gruczyńska, E., Ścibisz, I. and Rudzińska M. (2016). Fatty acids and sterols composition, and antioxidant activity of oils extracted from plant seeds, Food Chemistry, 213, 450-456.
  • Sujith Kumar, M.S., Mawlong, I. and Singh, D. (2017). Phytosterol recovery from oilseeds: Recent advances, Journal of Food Process Engineer, 40 (3), e12466.
  • Li, D., Park, J. and Oh, J. R. (2001). Silyl derivatization of alkylphenols, chlorophenols, and bisphenol A for simultaneous GC/MS determination, Analytical Chemistry, 73(13), 3089-3095.
  • Orem, A., Alasalvar, C., Kural, B. V., Yaman, S., Orem, C., Karadag, A., ... & Zawistowski, J. (2017). Cardio-protective effects of phytosterol-enriched functional black tea in mild hypercholesterolemia subjects, Journal of Functional Foods, 31, 311-319.
  • Piironen, V., Toivo, J. and Lampi, A.M. (2000). Natural sources of dietary plant sterols, Journal of Food Compositon and Analysis, 13, 619-624.
  • Poojary M.M. and Passamonti P. (2016). Improved conventional and microwave-assisted silylation protocols for simultaneous gas chromatographic determination of tocopherols and sterols: Method development and multi-response optimization, Journal of Chromatography A, 9, 88-104.
  • SAS (2000). SAS Institute Inc.: SAS Version 8.02: SAS/STAT Software: changes and enhancements through Release 8.02. Cary, NC, SAS Institute Inc, USA.
  • Rideout, T.C., Carrier, B., Wen, S., Raslawsky, A., Browne, R.W. and Harding, S.V. (2016). Complementary cholesterol-lowering response of a phytosterol/α-lipoic acid combination in obese Zucker rats, Journal of Dietary Supplements, 13, 283-299.
  • Uitterhaegen, E., Sampaio, K.A., Delbeke, E.I.P., De Greyt, W., Cerny, M., Evon, P., Merah, O., Talou, T. and Stevens, C.V. (2016). Characterization of French coriander oil as source of petroselinic acid, Molecules, 2, 1202.
  • Yang, R., Zhang, L., Li, P., Yu, L., Mao, J., Wang, X. and Zhang, Q. (2018). A review of chemical composition and nutritional properties of minor vegetable oils in China, Trends in Food Science & Technology, 74, 26-32,
  • Wojciechowski, Z. A. (1991) Biochemistry of phytosterol conjugates, in Physiology and Biochemistry of Sterols (Patterson, G.W. and Nes, W.D., eds), pp. 361–395, American Oil Chemists' Society.
There are 22 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Erman Beyzi 0000-0002-0248-4227

Selma Büyükkılıç Beyzi 0000-0002-4622-0645

Kevser Karaman 0000-0003-0729-6185

Project Number FHD-2017-7086
Publication Date December 31, 2019
Published in Issue Year 2019 Issue: 17

Cite

APA Beyzi, E., Büyükkılıç Beyzi, S., & Karaman, K. (2019). Sterol Profile of Some Medicinal and Aromatic Plant Oils: Effect of Silyl Derivatization Process. Avrupa Bilim Ve Teknoloji Dergisi(17), 360-365. https://doi.org/10.31590/ejosat.597071

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