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Impact of Growth Regulators on Rapid Clonal Propagation of Rotala rotundifolia (Buch-Ham. ex Roxb) in Liquid Culture Medium

Year 2022, Volume: 18 Issue: 2, 79 - 86, 31.12.2022

Muhammet Doğan

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb) is water plant used for medicinal and ornamental purposes. The aim of the work was to investigate the impacts of different growth regulators on the in vitro clonal propagation of R. rotundifolia. All assays were performed in liquid Murashige and Skoog (MS) media (without agar). Nodal explants were transferred to liquid MS nutrient media. Different combinations of Gibberellic Acid (GA3) + Thidiazuron (TDZ) and Kinetin (KIN) + Gibberellic Acid (GA3) were added to the nutrient solutions as growth regulators. The best results in terms of shoot numbers and shoot lengths were determined in nutrient solution including 0.25 mg/L GA3+ 0.25 mg/L TDZ and 0.25 mg/L KIN+ 0.25 mg/L GA3. When both hormone combinations were compared, higher shoot numbers were found in nutrient solutions including GA3+TDZ. On the other hand, longer shoots were obtained in nutrient solutions including KIN+GA3 After the propagation trials, the plants formed roots in the media with 0.25 mg/L indole-3-butyric acid (IBA). The plants that have been produced have been successfully adapted to the aquarium.

Keywords

in vitro propagation medicinal plant MS medium nodal explant tissue culture

Supporting Institution

Technological Research Council of Turkey (TUBITAK)

Project Number

2130190

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant (Project no: 2130190).

References

  • [1]. Oseni, O. M., Pande, V., Nailwal, T. K., “A review on plant tissue culture, a technique for propagation and conservation of endangered plant species”, International Journal of Current Microbiology and Applied Sciences, 7(7): 3778-3786, 2018.
  • [2]. Bidabadi, S. S., Jain, S. M., “Cellular, molecular, and physiological aspects of in vitro plant regeneration”, Plants, 9(6): 702, 2020.
  • [3]. Murashige, T., Skoog, F., “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiologia Plantarum, 15: 473-497, 1962.
  • [4]. Phillips, G. C., Garda, M., “Plant tissue culture media and practices: an overview”, In Vitro Cellular & Developmental Biology-Plant, 55(3): 242-257, 2019.
  • [5]. Akin, M., Eyduran, S. P., Eyduran, E., Reed, B. M., “Analysis of macro nutrient related growth responses using multivariate adaptive regression splines”, Plant Cell, Tissue and Organ Culture (PCTOC), 140(3): 661-670, 2020.
  • [6]. Abubakar, A. S., Yahaya, S. U., Shaibu, A. S., Ibrahim, H., Ibrahim, A. K., Lawan, Z. M., Isa, A. M., “In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars”, Agricultural Science Digest-A Research Journal, 38(1): 17-21, 2018.
  • [7]. Jing, C. H. E. N., “Efficient somatic embryogenesis and plant regeneration through callus initiation from seedling-derived leaf materials of maize (Zea mays L.)”, Agricultural Biotechnology, 8(3): 2164-4993, 2019.
  • [8]. Zong, X., Chen, Q., Nagaty, M. A., Kang, Y., Lang, G., Song, G. Q., “Adventitious shoot regeneration and Agrobacterium tumefaciens-mediated transformation of leaf explants of sweet cherry (Prunus avium L.)”, The Journal of Horticultural Science and Biotechnology, 94(2): 229-236, 2019.
  • [9]. Pérez-Caselles, C., Faize, L., Burgos, L., Alburquerque, N., “Improving adventitious shoot regeneration and transient Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) hypocotyl sections”, Agronomy, 11(7): 1338, 2021.
  • [10]. Debnath, S. C., Arigundam, U., “In vitro Propagation strategies of medicinally important berry crop, lingonberry (Vaccinium vitis-idaea L.)”, Agronomy, 10(5): 744, 2020.
  • [11]. Dogan, M., Emsen, B., “Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes”, Eurasian Journal of Biological and Chemical Sciences, 1(2): 48-53, 2018.
  • [12]. Dogan, M., “In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze”, Anatolian Journal of Botany, 3(1): 1-6, 2019.
  • [13]. Talla, S. K., Madam, E., Manga, S., Aileni, M., Mamidala, P., “Efficient TDZ-induced regeneration from capitulum explants of Gerbera jamesonii Bolus ex Hooker F.-an ornamental plant with high aesthetic value”, Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 153(5): 679-685, 2019.
  • [14]. Yang, Y., Zhang, X., Xiao, J., Chu, S., Huang, Z., Nutrient removal by Rotala rotundifolia: a superior candidate for ecosystem remediation at low temperatures”, RSC Advances, 10(49): 29139-29146, 2020.
  • [15]. Chen, Y. M., Huang, J. Z., Hou, T. W., Pan, I. C., “Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia”, Botanical studies, 60(1): 1-8, 2019.
  • [16]. Karakas, F. P., “Efficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulators”, Plant Physiology and Biochemistry, 146: 384-391, 2020.
  • [17]. Nowakowska, K., Pacholczak, A., Tepper, W., “The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L.‘Alba’, Rendiconti Lincei. Scienze Fisiche e Naturali, 30(1): 197-205, 2019.
  • [18]. Amghar, I., Diria, G., Boumlik, I., Gaboun, F., Iraqi, D., Labhilili, M., ... Abdelwahd, R., “An efficient regeneration pathway through adventitious organogenesis for the endangered Argania spinosa (L.) Skeels”, Vegetos, 1-13, 2021.
  • [19]. Ahmad, A., Ahmad, N., Anis, M., Alatar, A. A., Abdel-Salam, E. M., Qahtan, A. A., Faisal, M., “Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings”, Plant Cell, Tissue and Organ Culture (PCTOC), 144(2): 449-462, 2021.
  • [20]. Mei-Yin, C., Sani, H., “In vitro plantlet regeneration from nodal explant and callus induction of Vernonia amygdalina Delile”, Journal of Plant Sciences, 6(1): 1-6, 2018.
  • [21]. Dogan, M., “In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants”, Journal of the Institute of Science and Technology, 8: 77-84, 2018.
  • [22]. Foo, P. C., Lee, Z. H., Chin, C. K., Subramaniam, S., Chew, B. L., “Shoot induction in white eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-benzylaminopurine and kinetin”, Tropical Life Sciences Research, 29(2): 119, 2018.
  • [23]. Shukla, S., Prasad, Y., Yadav, A. K., Dixit, P., Patel, B., Yadav, V., “Effect of different auxins and cytokinins in callus induction and shoot regeneration in banana (Musa paradisiaca L.) variety Udhayam under in vitro condition”, International Journal of Chemical Studies, 6(4): 2041-2045, 2018.
  • [24]. Unal, S., Turkmen, G., Yagmur, B., Bayraktar, M., Gurel, A., “Improved in vitro propagation and direct acclimatization of Cryptocoryne wendtii in aquarium in the presence of aquarium fish Puntius tetrazona (Bleeker)”, Indian Journal of Experimental Biology, 57: 330-337, 2019.
  • [25]. Haque, S. M., Ghosh, B., “A submerged culture system for rapid micropropagation of the commercially important aquarium plant,‘Amazon sword’(Echinodorus ‘Indian Red’)”, In Vitro Cellular & Developmental Biology-Plant, 55(1): 81-87, 2019.

Sıvı Kültür Ortamında Büyüme Düzenleyicilerinin Rotala rotundifolia (Buch-Ham. Ex Roxb)’nın Hızlı Klonal Çoğaltımı Üzerine Etkisi

Year 2022, Volume: 18 Issue: 2, 79 - 86, 31.12.2022

Muhammet Doğan

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb) tıbbi ve süs amaçlı kullanılan önemli bir su bitkisidir. Bu çalışmanın amacı farklı büyüme düzenleyicilerinin R. rotundifolia’nın in vitro klonal çoğaltımı üzerine farklı büyüme düzenleyicilerinin etkilerini araştırmaktır. Tüm dememeler sıvı Murashige ve Skoog (MS) ortamında yürütülmüştür (agarsız). Boğum eksplantları sıvı besin ortamlarına aktarılmıştır. Besin solüsyonlarına büyüme düzenleyici olarak farklı kombinasyonlarda Gibberellik asit (GA3) + Thidiazuron (TDZ) ve Kinetin (KIN) + Gibberellik asit (GA3) eklenmiştir. Sürgün sayısı ve uzunlukları açısından en iyi sonuçlar 0.25 mg/L GA3 + 0.25 mg/L TDZ ve 0.25 mg/L KIN+ 0.25 mg/L GA3 içeren besin solüsyonunda belirlenmiştir. Her iki hormon kombinasyonları karşılaştırıldığında, daha fazla sürgün sayıları GA3+TDZ içeren besin solüsyonlarında bulunmuştur. Buna karşın daha uzun sürgünler KIN+GA3 içeren besin solüsyonlarında elde edilmiştir. Çoğaltım denemelerinden sonra bitkiler 0.25 mg/L indol-3-bütirik asit (IBA)’lı ortamda kök oluşturdu. Üretimi tamamlanan bitkiler akvaryuma başarıyla adapte edilmiştir.

Keywords

in vitro çoğaltım tıbbi bitki MS medium boğum eksplant doku kültürü

Project Number

2130190

References

  • [1]. Oseni, O. M., Pande, V., Nailwal, T. K., “A review on plant tissue culture, a technique for propagation and conservation of endangered plant species”, International Journal of Current Microbiology and Applied Sciences, 7(7): 3778-3786, 2018.
  • [2]. Bidabadi, S. S., Jain, S. M., “Cellular, molecular, and physiological aspects of in vitro plant regeneration”, Plants, 9(6): 702, 2020.
  • [3]. Murashige, T., Skoog, F., “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiologia Plantarum, 15: 473-497, 1962.
  • [4]. Phillips, G. C., Garda, M., “Plant tissue culture media and practices: an overview”, In Vitro Cellular & Developmental Biology-Plant, 55(3): 242-257, 2019.
  • [5]. Akin, M., Eyduran, S. P., Eyduran, E., Reed, B. M., “Analysis of macro nutrient related growth responses using multivariate adaptive regression splines”, Plant Cell, Tissue and Organ Culture (PCTOC), 140(3): 661-670, 2020.
  • [6]. Abubakar, A. S., Yahaya, S. U., Shaibu, A. S., Ibrahim, H., Ibrahim, A. K., Lawan, Z. M., Isa, A. M., “In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars”, Agricultural Science Digest-A Research Journal, 38(1): 17-21, 2018.
  • [7]. Jing, C. H. E. N., “Efficient somatic embryogenesis and plant regeneration through callus initiation from seedling-derived leaf materials of maize (Zea mays L.)”, Agricultural Biotechnology, 8(3): 2164-4993, 2019.
  • [8]. Zong, X., Chen, Q., Nagaty, M. A., Kang, Y., Lang, G., Song, G. Q., “Adventitious shoot regeneration and Agrobacterium tumefaciens-mediated transformation of leaf explants of sweet cherry (Prunus avium L.)”, The Journal of Horticultural Science and Biotechnology, 94(2): 229-236, 2019.
  • [9]. Pérez-Caselles, C., Faize, L., Burgos, L., Alburquerque, N., “Improving adventitious shoot regeneration and transient Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) hypocotyl sections”, Agronomy, 11(7): 1338, 2021.
  • [10]. Debnath, S. C., Arigundam, U., “In vitro Propagation strategies of medicinally important berry crop, lingonberry (Vaccinium vitis-idaea L.)”, Agronomy, 10(5): 744, 2020.
  • [11]. Dogan, M., Emsen, B., “Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes”, Eurasian Journal of Biological and Chemical Sciences, 1(2): 48-53, 2018.
  • [12]. Dogan, M., “In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze”, Anatolian Journal of Botany, 3(1): 1-6, 2019.
  • [13]. Talla, S. K., Madam, E., Manga, S., Aileni, M., Mamidala, P., “Efficient TDZ-induced regeneration from capitulum explants of Gerbera jamesonii Bolus ex Hooker F.-an ornamental plant with high aesthetic value”, Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 153(5): 679-685, 2019.
  • [14]. Yang, Y., Zhang, X., Xiao, J., Chu, S., Huang, Z., Nutrient removal by Rotala rotundifolia: a superior candidate for ecosystem remediation at low temperatures”, RSC Advances, 10(49): 29139-29146, 2020.
  • [15]. Chen, Y. M., Huang, J. Z., Hou, T. W., Pan, I. C., “Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia”, Botanical studies, 60(1): 1-8, 2019.
  • [16]. Karakas, F. P., “Efficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulators”, Plant Physiology and Biochemistry, 146: 384-391, 2020.
  • [17]. Nowakowska, K., Pacholczak, A., Tepper, W., “The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L.‘Alba’, Rendiconti Lincei. Scienze Fisiche e Naturali, 30(1): 197-205, 2019.
  • [18]. Amghar, I., Diria, G., Boumlik, I., Gaboun, F., Iraqi, D., Labhilili, M., ... Abdelwahd, R., “An efficient regeneration pathway through adventitious organogenesis for the endangered Argania spinosa (L.) Skeels”, Vegetos, 1-13, 2021.
  • [19]. Ahmad, A., Ahmad, N., Anis, M., Alatar, A. A., Abdel-Salam, E. M., Qahtan, A. A., Faisal, M., “Gibberellic acid and thidiazuron promote micropropagation of an endangered woody tree (Pterocarpus marsupium Roxb.) using in vitro seedlings”, Plant Cell, Tissue and Organ Culture (PCTOC), 144(2): 449-462, 2021.
  • [20]. Mei-Yin, C., Sani, H., “In vitro plantlet regeneration from nodal explant and callus induction of Vernonia amygdalina Delile”, Journal of Plant Sciences, 6(1): 1-6, 2018.
  • [21]. Dogan, M., “In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants”, Journal of the Institute of Science and Technology, 8: 77-84, 2018.
  • [22]. Foo, P. C., Lee, Z. H., Chin, C. K., Subramaniam, S., Chew, B. L., “Shoot induction in white eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-benzylaminopurine and kinetin”, Tropical Life Sciences Research, 29(2): 119, 2018.
  • [23]. Shukla, S., Prasad, Y., Yadav, A. K., Dixit, P., Patel, B., Yadav, V., “Effect of different auxins and cytokinins in callus induction and shoot regeneration in banana (Musa paradisiaca L.) variety Udhayam under in vitro condition”, International Journal of Chemical Studies, 6(4): 2041-2045, 2018.
  • [24]. Unal, S., Turkmen, G., Yagmur, B., Bayraktar, M., Gurel, A., “Improved in vitro propagation and direct acclimatization of Cryptocoryne wendtii in aquarium in the presence of aquarium fish Puntius tetrazona (Bleeker)”, Indian Journal of Experimental Biology, 57: 330-337, 2019.
  • [25]. Haque, S. M., Ghosh, B., “A submerged culture system for rapid micropropagation of the commercially important aquarium plant,‘Amazon sword’(Echinodorus ‘Indian Red’)”, In Vitro Cellular & Developmental Biology-Plant, 55(1): 81-87, 2019.
There are 25 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Articles
Authors

Muhammet Doğan 0000-0003-3138-5903

Project Number 2130190
Publication Date December 31, 2022
Submission Date January 17, 2022
Published in Issue Year 2022 Volume: 18 Issue: 2

Cite

APA Doğan, M. (2022). Impact of Growth Regulators on Rapid Clonal Propagation of Rotala rotundifolia (Buch-Ham. ex Roxb) in Liquid Culture Medium. Electronic Letters on Science and Engineering, 18(2), 79-86.
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