Research Article
Development of methodology for resistance screening of chickpea genotypes collected in Turkey to the root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae)
Abstract
The root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) are considered economically important plant parasitic nematodes affecting chickpea [Cicer arietinum L. (Fabales: Fabaceae)] production. A major strategy to develop resistance to root lesion nematodes in chickpea is to assess and exploit their natural variation. Therefore, nine accessions of wild Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis (Fabales: Fabaceae) and domesticate C. arietinum were assessed for resistance to P. thorneiaccording to multiplication rate of the nematode. This study was conducted during 2014-2015 to detect the suitable initial inoculum density (150, 225 and 300) per plant duration of experiment (16 and 20 weeks) for resistance test to P. thornei in chickpea cultivars. There was no significant difference between growing times 16 and 20 weeks and between the initial inoculum density of 225 and 300 nematodes. The only significant difference was observed at a low initial inoculum density of 150 nematodes in all tested cultivars. Therefore, the initial inoculum density of 225 and the growing time of 16 weeks were selected to access of chickpea genotypes for resistance study to P. thornei.
Supporting Institution
GRDC
Project Number
CSP00185
Thanks
We thank Dr. Abdullah Kahraman (Department of Field Crops, Agriculture Faculty, Harran University) for his help and provision of chickpea seeds.
References
- Atkinson, H. J., E. P. Urwin, E. Hansen & M. J. McPherson, 1995. Designs for engineered resistance to root-parasitic nematodes. Trends Biotechnology, 13: 369-374.
- Barker, K. R. & J. P. Noe, 1987. “Establishing and Using Threshold Population Levels, 75-81” In: Vistas on Nematology (Eds. J. A. Veech & S. W. Dickson). Society of Nematologists, Maryland, USA, 300 pp.
- Behmand T., N. Z. Elekcioğlu, J. Berger & I. H. Elekcioğlu, 2019. Determination of plant-parasitic nematodes associated with chickpea in Turkey. Turkish Journal of Entomology, 43 (4): 357-366.
- Castillo, P. & N. Vovlas, 2007. Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management. Nematology Monographs and Perspectives, Brill Leiden-Boston, The Netherlands-USA, 6: 529 pp.
- Castillo, P., N. Vovlas & R. M. Jimenez-Diaz,1998. Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea genotypes. Plant Pathology, 47: 370-376.
- FAO, 2019. Food and Agriculture Organization of the United Nations Statistical Data. (Web page: www.fao.org/faostat) (Date accessed: 24 April 2019).
- Hooper, D. J., 1986. “Extraction of Free-Living Stages from Soil, 5-30”. In: Laboratory Methods for Work with Plant and Soil Nematodes (Ed. J. F. Southey). Ministry of Agriculture, Fisheries, and Food Technology, Reference Book 402, Her Majesty’s Stationary Office, London, UK, 629 pp.
- Jatala, P. & J. Bridge, 1990. “Nematode Parasites of Root and Tuber Crops, 137-180”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. M. Luc, A. Sikora & J. Bridge). CAB International, Wallingford, UK, 221 pp.
- Nicol, J., R. Rivoal & S. Taylor, 2004. Global importance of cyst (Heterodera spp.) and lesion nematodes (Pratylenchus spp.) on cereals: yield loss, population dynamics, use of host resistance and integration of molecular tools. Nematology Monographs and Perspectives, 2: 233-251.
- Nicol, J. M. & V. A. Vanstone, 1993. “Carrot piece and chickpea callus culture for Pratylenchus thornei and P. neglectus, 254-264”. In: Proceedings of the Pratylenchus Workshop, 9th Biennial Conference of the Australasian Plant Pathology Society (8-9 July 1993, Hobart, Tasmania, Australia), 347 pp.
- Oostenbrink, M., 1966. Major characteristics of the relation between nematodes and plants. Mededelingen Landbouwhogeschool Wageningen, 66: 1-46.
- Reen, R. A. & J. P. Thompson, 2009. “A comparative study of methods for screening chickpea and wheat for resistance to root-lesion nematode Pratylenchus thornei, 119-120”. In: Proceedings of Plant Health Management an Integrated Approach, 17th Biennial Australasian Plant Pathology Society Conference (29 September–1 October 2009, Newcastle), 196 pp.
- Sasser, J. N. & D. W. Freckman, 1987. “A world perspective on nematology: the role of the society, 7-14”. In: Vistas on Nematology: A Commemoration of the 25th Anniversary of the Society of Nematologists (Eds. J. A. Veech & S. W. Dickson). Society of Nematologists, Hyattsville, MD, USA, 299 pp.
- Schomaker, C. H. & T. H. Been, 2006. “Plant Growth and Population Dynamics, 275-295". In: Plant Nematology (Eds. R. Perry & M. Moens). CAB International, Wallingford, UK, 536 pp.
- Seinhorst, J. W.,1965. The relation between nematode density and damage to plants. Nematologica, 11: 137-154.
- Sikora, R. A., M. Luc & J. Bridge, 2005. “Nematode Parasites of Food Legumes, 259-318". In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (2nd Edition) (Eds. M. Luc, R.A. Sikora & J. Bridge) CABI Publishing, Wallingford, UK, 871 pp.
- Singh, K. B. & B. Ocampo, 1997. Exploitation of wild Cicer species for yield improvement in chickpea. Theoretical and Applied Genetics, 95: 418-423.
- Smýkal, P., C. J. Coyne, M. J. Ambrose, N. Maxted, H. Schaefer, M. W. Blair, J. Berger, S. L. Greene, M. N. Nelson, N. Besharat, T. Vymyslický, C. Toker, R. K. Saxena, M. Roorkiwal, M. K. Pandey, J. Hu, Y. H. Li, L. X. Wang, Y. Guo, L. J. Qiu, R. J. Redden & R. K. Varshney, 2015. Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences, 34 (1-3): 43-104.
- Tanha Maafi, Z., J. M. Nicol, H. Kazemi, N. Ebrahimi, M. Gitty, M. Ghalandar, M. Mohammadi-Pour & Z. Khoshkhabar, 2009. “Cereal cyst nematodes, root rot pathogens and root lesion nematodes affecting cereal production in Iran, 51-55". In: Cereal Cyst Nematodes: Status, Research and Outlook (Eds. I. T. Riley, J. M. Nicol & A. A. Dababat). CIMMYT, Ankara, Turkey, 245 pp.
- Taylor, S. P., G. J. Hollaway & C. H. Hunt, 2000. Effect of field crops on population densities of Pratylenchus neglectus and P. thornei in southeastern Australia; Part 1 P. neglectus. Journal of Nematology, 32: 591-599.
- Thompson, J. P., T. G. Clewett & J. G. Sheedy, 2010. Occurrence of root-lesion nematodes (Pratylenchus thornei and P. neglectus) and stunt nematode (Merlinius brevidens) in the northern grain region of Australia. Australasian Plant Pathology, 39: 254-264.
- Thompson, J. P., N. Greco, R. Eastwood, S. B. Sharma & M. Scurrah, 2000. “Integrated control of nematodes of cool-season food legumes, 491-506". In: Linking Research and Marketing Opportunities for Pulses in the 21st Century (Ed. R. Knight). Proceedings Third International Food Legumes Research Conference (22–26 September 1997, Australia), Kluwer Academic Publishers, Dordrecht, the Netherlands, 611 pp.
- Thompson, J. P., J. MacKenzie & R. Amos, 1995. Root lesion nematode (Pratylenchus thornei) limits the response of wheat but not barley to stored soil moisture in the Hermitage long-term tillage experiment. Australian Journal of Experimental Agriculture, 35: 149-155.
- Thompson, J. P., R. A. Reen, T. G. Clewett, J. G. Sheedy, A. M. Kelly, B. J. Gogel & E. J. Knights, 2011. Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus). Australasian Plant Pathology, 40 (6): 601-611.
- Toktay, H., M. İmren, J. Nicol, A. Dababat & İ. H. Elekcioğlu, 2012. Improved methodology for resistance screening in spring wheat against the root lesion nematode, Pratylenchus thornei (Sher et Allen) (Tylenchida: Pratylenchidae). Turkish Journal of Entomology, 36 (4): 533-540.
- Trudgill, D. L., 1992. Resistance to and tolerance of plant-parasitic nematodes in plants. Annual Review of Phytopathology, 29: 167-192.
- Vanstone, V. A., A. J. Rathjen, A. H. Ware & R. D. Wheeler, 1998. Relationship between root lesion nematodes (Pratylenchus negleclus and P. thornei) and performance of wheat varieties. Australian Journal of Experimental Agriculture, 38: 181-188.
Türkiye'den toplanan nohut genotiplerinin kök yara nematoduna, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) karşı taranması
Abstract
Kök yara nematodu, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) dünyada nohut [Cicer arietinum L. (Fabales: Fabaceae)] üretimini etkileyen, ekonomik açıdan önemli bitki paraziti nematod türü olarak kabul edilmektedir. Nohutta kök yara nematodlarına karşı direnç geliştirmenin temel stratejisi, doğal çeşitliliklerini değerlendirmek ve kullanmaktır. Bundan dolayı P. thornei'ye karşı dayanıklılık çalışmalarında, üreme oranı dikkate alınarak, dokuz adet yabani Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis(Fabales: Fabaceae) ve yerli C. arietinum türleri değerlendirilmiştir. Bu çalışma, nohut çeşitlerinin P. thornei'ye karşı dayanıklılık denemeleri için, uygun hasat süresi (16 ve 20 hafta) ve bitki başına başlangıç inokulum yoğunluğunu (150, 225 ve 300) belirlemek için 2014-2015 yılları arasında yürütülmüştür. Hasat zamanı 16 ve 20 hafta ile 225 ve 300 başlangıç inokulum yoğunluğu arasında anlamlı bir fark olmadığı, buna karşın bitki başına 150 inokulum yoğunluğunun istatistiki olarak daha düşük etki gösterdiği saptanmıştır. Bu nedenle, nohut çeşitlerinde P. thornei'ye karşı dayanıklılık çalışmaları için en uygun deneme parametrelerinin, 225 başlangıç inokulum yoğunluğu ve 16 hafta hasat zamanı olduğu belirlenmiştir.
Project Number
CSP00185
References
- Atkinson, H. J., E. P. Urwin, E. Hansen & M. J. McPherson, 1995. Designs for engineered resistance to root-parasitic nematodes. Trends Biotechnology, 13: 369-374.
- Barker, K. R. & J. P. Noe, 1987. “Establishing and Using Threshold Population Levels, 75-81” In: Vistas on Nematology (Eds. J. A. Veech & S. W. Dickson). Society of Nematologists, Maryland, USA, 300 pp.
- Behmand T., N. Z. Elekcioğlu, J. Berger & I. H. Elekcioğlu, 2019. Determination of plant-parasitic nematodes associated with chickpea in Turkey. Turkish Journal of Entomology, 43 (4): 357-366.
- Castillo, P. & N. Vovlas, 2007. Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management. Nematology Monographs and Perspectives, Brill Leiden-Boston, The Netherlands-USA, 6: 529 pp.
- Castillo, P., N. Vovlas & R. M. Jimenez-Diaz,1998. Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea genotypes. Plant Pathology, 47: 370-376.
- FAO, 2019. Food and Agriculture Organization of the United Nations Statistical Data. (Web page: www.fao.org/faostat) (Date accessed: 24 April 2019).
- Hooper, D. J., 1986. “Extraction of Free-Living Stages from Soil, 5-30”. In: Laboratory Methods for Work with Plant and Soil Nematodes (Ed. J. F. Southey). Ministry of Agriculture, Fisheries, and Food Technology, Reference Book 402, Her Majesty’s Stationary Office, London, UK, 629 pp.
- Jatala, P. & J. Bridge, 1990. “Nematode Parasites of Root and Tuber Crops, 137-180”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. M. Luc, A. Sikora & J. Bridge). CAB International, Wallingford, UK, 221 pp.
- Nicol, J., R. Rivoal & S. Taylor, 2004. Global importance of cyst (Heterodera spp.) and lesion nematodes (Pratylenchus spp.) on cereals: yield loss, population dynamics, use of host resistance and integration of molecular tools. Nematology Monographs and Perspectives, 2: 233-251.
- Nicol, J. M. & V. A. Vanstone, 1993. “Carrot piece and chickpea callus culture for Pratylenchus thornei and P. neglectus, 254-264”. In: Proceedings of the Pratylenchus Workshop, 9th Biennial Conference of the Australasian Plant Pathology Society (8-9 July 1993, Hobart, Tasmania, Australia), 347 pp.
- Oostenbrink, M., 1966. Major characteristics of the relation between nematodes and plants. Mededelingen Landbouwhogeschool Wageningen, 66: 1-46.
- Reen, R. A. & J. P. Thompson, 2009. “A comparative study of methods for screening chickpea and wheat for resistance to root-lesion nematode Pratylenchus thornei, 119-120”. In: Proceedings of Plant Health Management an Integrated Approach, 17th Biennial Australasian Plant Pathology Society Conference (29 September–1 October 2009, Newcastle), 196 pp.
- Sasser, J. N. & D. W. Freckman, 1987. “A world perspective on nematology: the role of the society, 7-14”. In: Vistas on Nematology: A Commemoration of the 25th Anniversary of the Society of Nematologists (Eds. J. A. Veech & S. W. Dickson). Society of Nematologists, Hyattsville, MD, USA, 299 pp.
- Schomaker, C. H. & T. H. Been, 2006. “Plant Growth and Population Dynamics, 275-295". In: Plant Nematology (Eds. R. Perry & M. Moens). CAB International, Wallingford, UK, 536 pp.
- Seinhorst, J. W.,1965. The relation between nematode density and damage to plants. Nematologica, 11: 137-154.
- Sikora, R. A., M. Luc & J. Bridge, 2005. “Nematode Parasites of Food Legumes, 259-318". In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (2nd Edition) (Eds. M. Luc, R.A. Sikora & J. Bridge) CABI Publishing, Wallingford, UK, 871 pp.
- Singh, K. B. & B. Ocampo, 1997. Exploitation of wild Cicer species for yield improvement in chickpea. Theoretical and Applied Genetics, 95: 418-423.
- Smýkal, P., C. J. Coyne, M. J. Ambrose, N. Maxted, H. Schaefer, M. W. Blair, J. Berger, S. L. Greene, M. N. Nelson, N. Besharat, T. Vymyslický, C. Toker, R. K. Saxena, M. Roorkiwal, M. K. Pandey, J. Hu, Y. H. Li, L. X. Wang, Y. Guo, L. J. Qiu, R. J. Redden & R. K. Varshney, 2015. Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences, 34 (1-3): 43-104.
- Tanha Maafi, Z., J. M. Nicol, H. Kazemi, N. Ebrahimi, M. Gitty, M. Ghalandar, M. Mohammadi-Pour & Z. Khoshkhabar, 2009. “Cereal cyst nematodes, root rot pathogens and root lesion nematodes affecting cereal production in Iran, 51-55". In: Cereal Cyst Nematodes: Status, Research and Outlook (Eds. I. T. Riley, J. M. Nicol & A. A. Dababat). CIMMYT, Ankara, Turkey, 245 pp.
- Taylor, S. P., G. J. Hollaway & C. H. Hunt, 2000. Effect of field crops on population densities of Pratylenchus neglectus and P. thornei in southeastern Australia; Part 1 P. neglectus. Journal of Nematology, 32: 591-599.
- Thompson, J. P., T. G. Clewett & J. G. Sheedy, 2010. Occurrence of root-lesion nematodes (Pratylenchus thornei and P. neglectus) and stunt nematode (Merlinius brevidens) in the northern grain region of Australia. Australasian Plant Pathology, 39: 254-264.
- Thompson, J. P., N. Greco, R. Eastwood, S. B. Sharma & M. Scurrah, 2000. “Integrated control of nematodes of cool-season food legumes, 491-506". In: Linking Research and Marketing Opportunities for Pulses in the 21st Century (Ed. R. Knight). Proceedings Third International Food Legumes Research Conference (22–26 September 1997, Australia), Kluwer Academic Publishers, Dordrecht, the Netherlands, 611 pp.
- Thompson, J. P., J. MacKenzie & R. Amos, 1995. Root lesion nematode (Pratylenchus thornei) limits the response of wheat but not barley to stored soil moisture in the Hermitage long-term tillage experiment. Australian Journal of Experimental Agriculture, 35: 149-155.
- Thompson, J. P., R. A. Reen, T. G. Clewett, J. G. Sheedy, A. M. Kelly, B. J. Gogel & E. J. Knights, 2011. Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus). Australasian Plant Pathology, 40 (6): 601-611.
- Toktay, H., M. İmren, J. Nicol, A. Dababat & İ. H. Elekcioğlu, 2012. Improved methodology for resistance screening in spring wheat against the root lesion nematode, Pratylenchus thornei (Sher et Allen) (Tylenchida: Pratylenchidae). Turkish Journal of Entomology, 36 (4): 533-540.
- Trudgill, D. L., 1992. Resistance to and tolerance of plant-parasitic nematodes in plants. Annual Review of Phytopathology, 29: 167-192.
- Vanstone, V. A., A. J. Rathjen, A. H. Ware & R. D. Wheeler, 1998. Relationship between root lesion nematodes (Pratylenchus negleclus and P. thornei) and performance of wheat varieties. Australian Journal of Experimental Agriculture, 38: 181-188.
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Project Number | CSP00185 |
| Publication Date | March 1, 2020 |
| Submission Date | September 17, 2019 |
| Acceptance Date | November 18, 2019 |
| Published in Issue | Year 2020 Volume: 44 Issue: 1 |
