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Kuzey Kıbrıs arpa ve buğday üretim alanlarında Akdeniz tahıl kist nematodu, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae)’un tanımlanması ve genetik çeşitliliği

Year 2020, Volume: 44 Issue: 2, 273 - 281, 01.06.2020
https://doi.org/10.16970/entoted.661964

Abstract

Akdeniz tahıl kist nematodu, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) özellikle buğday ve arpada olmak üzere tahıllarda önemli zararlara neden olan bir bitki paraziti nematod olup, dünyada tahıl yetiştirilen alanların büyük çoğunluğunda tespit edilmiştir. Bu çalışmada, Kıbrıs’ın tahıl alanlarındaki patojen nematodları belirlemek için bir sürvey yapılmıştır. Gazimağusa, Girne, Güzelyurt ve Lefkoşa tahıl üretim alanlarından toplam 45 adet toprak ve kök örneği 2017 yılının mayıs ayı sonu-haziran ayı başı arasında tahıl hasadı öncesinde alınmıştır. Toprak ve kök örneklerinden Fenwick yöntemii kullanılarak kistler toplanılmış, 37 adet örnekte (%82) kiste rastlanılmış olup, tüm örnekler ribozomal DNA’nın ITS bölgesi kullanılarak, tahıl kist nematodu H. latipons moleküler düzeyde tanımlanarak belirlenmiştir. Çalışmada Kuzey Kıbrıs arpa ve buğday alanlarından elde edilen H. latipons örneklerine ait ITS bölgesi sekans dizilerinin Fas, Rusya, Suriye ve Türkiye’den elde edilen izolatlara oldukça benzerlik gösterdiği tespit edilmiştir. Kuzey Kıbrıs'ın Gazimağusa, Girne, Güzelyurt ve Lefkoşa illeri tahıl alanlarında H. latipons'un ilk olarak tespit edildiği bu çalışma, nematodun yaygınlığını ortaya koyan ve zararlının mücadelesinde başta dayanıklı çeşit kullanımı olmak üzere diğer mücadele yöntemlerinin uygulanmasında temel oluşturacak verileri içermektedir.

References

  • Abidou, H., A. El-Ahmed, J. M. Nicol, N. Bolat, R. Rivoal & A. Yahyaoui, 2005. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematologia Mediterránea, 33: 197-203.
  • Al-Banna, L., A. T. Ploeg, V. M. Williamson & I. Kaloshian, 2004. Discrimination of six Pratylenchus species using PCR and species-specific primers. Journal of Nematology, 36 (2): 142-146.
  • Baklawa, M., B. Niere, H. Heuer & S. Massoud, 2015. Characterisation of cereal cyst nematodes in Egypt based on morphometrics, RFLP and rDNA-ITS sequence analyses. Nematology, 17 (1): 103-115.
  • Chitwood, D. J., 2003. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science (Pesticide Science), 59 (6-7): 748-753.
  • Cohn, E. & R. Ausher, 1973. Longidorus cohni and Heterodera latipons, economic nematode pests of oats in Israel. Plant Disease Reporter, 57 (1): 53-54.
  • Dababat, A. A. & H. Fourie, 2018. “Nematode Parasites of Cereals, 163-221”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. R. A. Sikora, D. Coyne, J. Hallmann & P. Timper). CAB International, 898 pp.
  • Dababat, A. A., M. Imren, G. Erginbas-Orakci, S. Ashrafi, E. Yavuzaslanoglu, H. Toktay & T. Mekete, 2015 The importance and management strategies of cereal cyst nematodes, Heterodera spp., in Turkey. Euphytica, 202:173-188.
  • Fenwick, D. W., 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology, 18: 155-172.
  • Franklin, M. T., 1969. Heterodera latipons n. sp., a cereal cyst nematode from the Mediterranean Region. Nematologica, 15: 535-542.
  • Gabler, C., D. Sturhan, S. A. Subbotin & H. J. Rumpenhorst, 2000. Heterodera pratensis sp. n., a new cyst nematode of the H. avenae complex (Nematoda: Heteroderidae). Russian Journal of Nematology, 8 (2): 115-126.
  • Gair, R., P. L. Mathias & P. N. Harvey, 1969. Studies of cereal cyst-nematode populations and cereal yields under continuous or intensive culture. Annals of Applied Biology, 63: 503-512.
  • Ganguly, A. K. & U. Rao, 2003. “Application of Molecular Biology in Nematology, 1-14”. In: Advances in Nematology (Ed. P. C. Trivedi). Scientific Publishers (India), Jodhpur, New Delhi, India, 317pp.
  • Greco, N., N. Vovlas, A. Troccoli & R. N. Inserra, 2002. The Mediterraean Cereal Cyst Nematode, Heterodera latipons: A Menace to Cool Season Cereals of the United States. Florida Department of Agriculture & Consumer Services, Division of Plant Industry, Circular No: 221, 6 pp.
  • Imren, M., H. Toktay, A. Özarslandan, J. M. Nicol & İ. H. Elekcioğlu, 2012. Determination of the cereal cyst nematode species, Heterodera avenae group in cereal fields of South East Anatolia. Turkish Journal of Entomology, 36 (2): 265-276.
  • Imren, M., L. Waeyenberge, N. Viaene, İ. H. Elekcioğlu & A. Dababat, 2015. Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey. Turkish Journal of Agriculture and Forestry, 39 (1): 91-98.
  • Imren, M., Ş. Yildiz, H. Toktay, N. Duman & A. A. Dababat, 2018. Morphometric and genetic variability among Mediterranean cereal cyst nematode (Heterodera latipons) populations in Turkey. Turkish Journal of Zoology, 42 (6): 625-636.
  • Kyndt, T., P. Vieira, G. Gheysen & J. de Almeida-Engler, 2013. Nematode feeding sites: unique organs in plant roots. Planta, 238 (5): 807-818.
  • Madani, M., N. Vovlas, P. Castillo, S. A. Subbotin & M. Moens, 2004. Molecular characterization of cyst nematode Species (Heterodera spp.) from the Mediterranean Basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152 (4): 229-234.
  • McDonald, A. H. & J. M. Nicol, 2005. “Nematode Parasites of Cereals, 131-191”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. M. Luc, R. A. Sikora & J. Bridge). CAB International, Wallingford, UK, 896 pp.
  • Mokrini, F., N. Viaene, L. Waeyenberge, A. A. Dababat & M. Moens, 2017. Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis. Journal of Plant Protection Research, 57 (3): 219-227.
  • NCBI, 2019. National Center for Biotechnology Information. (Web page: www.ncbi.nlm.nih.gov) (Date accessed: December 2019).
  • Peng, D. L., D. Zhang, J. M. Nicol, S. L. Chen, L. Waeyenberge, M. Moens, H. L. Li, W. H. Tang & I. T. Riley, 2007. “Occurrence, distribution and research situation of cereal cyst nematode in China, 350-351”. Proceedings of the XVI International Plant Protection Congress (15-18 October 2007, Glasgow, Scotland, UK), 874 pp.
  • Philis, I., 1988a. Occurrence of Hederodera latipons on barley in Cyprus. Nematologia Mediterranea, 16: 223.
  • Philis, I., 1988b. “Presence and role of plant parasitic nematodes in cereals and food and forage legumes in Cyprus, 143-146”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Philis, J., 1995. An up-dated list of plant parasitic nematodes from Cyprus and their economic importance. Nematologia Mediterranea, 23 (2): 307-314.
  • Rivoal, R., S. Valette, S. Bekal, J. P. Gauthier & A. Yahyaoui, 2003. Genetic and phenotypic diversity in the graminaceous cyst nematode complex, inferred from PCR-RFLP of ribosomal DNA and morphometric analysis. European Journal of Plant Pathology, 109: 227-241.
  • Rumpenhorst, H. J., I. H. Elekçioğlu, D. Sturhan, G. Öztürk & S. Enneli, 1996. The Cereal Cyst Nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterranea, 24: 135-138.
  • Sahin, E., Nicol, İ. H. Elekcioğlu & R. Rivoal, 2010. Hatching of Heterodera filipjevi in controlled and natural temperature conditions in Turkey. Nematology, 12 (2): 193-200.
  • Saxena, M. E., A. A. EI-Moneim, O. F. Mamluk & S. B. Hanounik, 1988. “A review of nematology research in ICARDA, 69-84”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Sikora, R. A., 1988. “Plant parasitic nematodes of wheat and barley in temperate and semi-arid regions. A comparative analysis, 46-48”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Smiley, R. W. & J. M. Nicol, 2009. Nematodes which challenge global wheat production. Wheat Science and Trade, 171-187.
  • Smiley, R. W., G. P. Yan & Z. A. Handoo, 2008. First record of the cyst nematode Heterodera filipjevi on wheat in Oregon. Plant Disease, 92 (7): 1136-1136.
  • Sturhan, D. & E. Krall, 2002. Heterodera iri Mathews, 1971 a junior synonym of H. ustinovi Kirjanova, 1969. Russian Journal of Nematology, 10 (1): 55-57.
  • Subbotin, S. A., A. Mundo-Ocampo & J. G. Baldwin, 2010. Systematics of Cyst Nematodes (Nematode: Heteroderinae). Nematology Monographs and Perspectives, 8A. Leiden, the Netherlands, Brill, 364 pp.
  • Subbotin, S. A., D. Sturhan, H. J. Rumpenhorst & M. Moens, 2002. Description of the Australian cereal cyst nematode Heterodera australis sp. n. (Tylenchida: Heteroderidae). Russian Journal of Nematology, 10 (2): 139-148.
  • Subbotin, S. A., D. Sturhan, H. J. Rumpenhorst & M. Moens, 2003. Molecular and morphological characterization of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology, 5: 515-538.
  • Subbotin, S. A., A. Vierstraete, P. De Ley, J. Rowe, L. Waeyenberge, M. Moens & J. R. Vanfleteren, 2001. Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA. Molecular Phylogenetics and Evolution, 21: 1-16.
  • Subbotin, S. A., L. Waeyenberge, I. A. Molokanova & M. Moens, 1999. Identification of Heterodera avenae group species by morphometrics and rDNARFLPs. Nematology, 1: 195-207.
  • Szalanski, A. L., D. D. Sui, T. S. Harris & T. O. Powers, 1997. Identification of cyst nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 29 (3): 255-267.
  • Tamura, K. & M. Nei, 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3): 512-526.
  • Thompson, J. D., D. G. Higgins & T. J. Gibson, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22 (22): 4673-4680.
  • Toktay, H., M. İmren, A. Öcal, L. Waeyenberge, N. Viaene & A. Dababat, 2015. Incidence of cereal cyst nematodes in the East Anatolia Region in Turkey. Russian Journal of Nematology, 23 (1): 29-40.
  • Wouts, W. M. & D. Sturhan, 1995. Heterodera aucklandica sp. n. (Nematoda: Heteroderidae) from a New Zealand native grass, with notes on the species of the H. avenae group. New Zealand Journal of Zoology, 22 (2): 199-207.

Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus

Year 2020, Volume: 44 Issue: 2, 273 - 281, 01.06.2020
https://doi.org/10.16970/entoted.661964

Abstract

The Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) is a destructive plant-parasitic nematode on cereal crops in particularly wheat and barley. It has a global distribution with a severe negative impact on yield quantity. In this study, a survey was conducted to identify plant-parasitic nematodes in cereal-growing areas in Cyprus. Forty-five samples including roots and soil from the root zone of plants were collected from cereal fields located in Gazimağusa, Girne, Güzelyurt and Lefkoşa Provinces before crop harvesting from late-May and early-June in 2017. Cyst-forming nematodes were determined by Fenwick’s flotation and decanting techniques from 37 soil samples (82%). The internal transcribed spacer (ITS) regions of the ribosomal DNA of isolates were amplified and sequenced and subjected to a BLASTn search of the NCBI database for species identification, and the analyses showed that all samples were identified as H. latipons. Phylogenetic analyses based on ITS sequences revealed that H. latipons isolates from Northern Cyprus were closely related to isolates obtained from Morocco, Russia, Syria and Turkey. Data of this study demonstrated for the first time the presence of H. latipons in the cereal fields of Gazimağusa, Girne, Güzelyurt and Lefkoşa Provinces, where the nematode most likely causes serious economic problems in the cereal production. These results were the most up-to-dated analyses on the occurrence of H. latipons in cereal fields of Northern Cyprus and provided basic data for breeding programs to improve the resistant levels in the local cultivars.

References

  • Abidou, H., A. El-Ahmed, J. M. Nicol, N. Bolat, R. Rivoal & A. Yahyaoui, 2005. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey. Nematologia Mediterránea, 33: 197-203.
  • Al-Banna, L., A. T. Ploeg, V. M. Williamson & I. Kaloshian, 2004. Discrimination of six Pratylenchus species using PCR and species-specific primers. Journal of Nematology, 36 (2): 142-146.
  • Baklawa, M., B. Niere, H. Heuer & S. Massoud, 2015. Characterisation of cereal cyst nematodes in Egypt based on morphometrics, RFLP and rDNA-ITS sequence analyses. Nematology, 17 (1): 103-115.
  • Chitwood, D. J., 2003. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science (Pesticide Science), 59 (6-7): 748-753.
  • Cohn, E. & R. Ausher, 1973. Longidorus cohni and Heterodera latipons, economic nematode pests of oats in Israel. Plant Disease Reporter, 57 (1): 53-54.
  • Dababat, A. A. & H. Fourie, 2018. “Nematode Parasites of Cereals, 163-221”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. R. A. Sikora, D. Coyne, J. Hallmann & P. Timper). CAB International, 898 pp.
  • Dababat, A. A., M. Imren, G. Erginbas-Orakci, S. Ashrafi, E. Yavuzaslanoglu, H. Toktay & T. Mekete, 2015 The importance and management strategies of cereal cyst nematodes, Heterodera spp., in Turkey. Euphytica, 202:173-188.
  • Fenwick, D. W., 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology, 18: 155-172.
  • Franklin, M. T., 1969. Heterodera latipons n. sp., a cereal cyst nematode from the Mediterranean Region. Nematologica, 15: 535-542.
  • Gabler, C., D. Sturhan, S. A. Subbotin & H. J. Rumpenhorst, 2000. Heterodera pratensis sp. n., a new cyst nematode of the H. avenae complex (Nematoda: Heteroderidae). Russian Journal of Nematology, 8 (2): 115-126.
  • Gair, R., P. L. Mathias & P. N. Harvey, 1969. Studies of cereal cyst-nematode populations and cereal yields under continuous or intensive culture. Annals of Applied Biology, 63: 503-512.
  • Ganguly, A. K. & U. Rao, 2003. “Application of Molecular Biology in Nematology, 1-14”. In: Advances in Nematology (Ed. P. C. Trivedi). Scientific Publishers (India), Jodhpur, New Delhi, India, 317pp.
  • Greco, N., N. Vovlas, A. Troccoli & R. N. Inserra, 2002. The Mediterraean Cereal Cyst Nematode, Heterodera latipons: A Menace to Cool Season Cereals of the United States. Florida Department of Agriculture & Consumer Services, Division of Plant Industry, Circular No: 221, 6 pp.
  • Imren, M., H. Toktay, A. Özarslandan, J. M. Nicol & İ. H. Elekcioğlu, 2012. Determination of the cereal cyst nematode species, Heterodera avenae group in cereal fields of South East Anatolia. Turkish Journal of Entomology, 36 (2): 265-276.
  • Imren, M., L. Waeyenberge, N. Viaene, İ. H. Elekcioğlu & A. Dababat, 2015. Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey. Turkish Journal of Agriculture and Forestry, 39 (1): 91-98.
  • Imren, M., Ş. Yildiz, H. Toktay, N. Duman & A. A. Dababat, 2018. Morphometric and genetic variability among Mediterranean cereal cyst nematode (Heterodera latipons) populations in Turkey. Turkish Journal of Zoology, 42 (6): 625-636.
  • Kyndt, T., P. Vieira, G. Gheysen & J. de Almeida-Engler, 2013. Nematode feeding sites: unique organs in plant roots. Planta, 238 (5): 807-818.
  • Madani, M., N. Vovlas, P. Castillo, S. A. Subbotin & M. Moens, 2004. Molecular characterization of cyst nematode Species (Heterodera spp.) from the Mediterranean Basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152 (4): 229-234.
  • McDonald, A. H. & J. M. Nicol, 2005. “Nematode Parasites of Cereals, 131-191”. In: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. M. Luc, R. A. Sikora & J. Bridge). CAB International, Wallingford, UK, 896 pp.
  • Mokrini, F., N. Viaene, L. Waeyenberge, A. A. Dababat & M. Moens, 2017. Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis. Journal of Plant Protection Research, 57 (3): 219-227.
  • NCBI, 2019. National Center for Biotechnology Information. (Web page: www.ncbi.nlm.nih.gov) (Date accessed: December 2019).
  • Peng, D. L., D. Zhang, J. M. Nicol, S. L. Chen, L. Waeyenberge, M. Moens, H. L. Li, W. H. Tang & I. T. Riley, 2007. “Occurrence, distribution and research situation of cereal cyst nematode in China, 350-351”. Proceedings of the XVI International Plant Protection Congress (15-18 October 2007, Glasgow, Scotland, UK), 874 pp.
  • Philis, I., 1988a. Occurrence of Hederodera latipons on barley in Cyprus. Nematologia Mediterranea, 16: 223.
  • Philis, I., 1988b. “Presence and role of plant parasitic nematodes in cereals and food and forage legumes in Cyprus, 143-146”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Philis, J., 1995. An up-dated list of plant parasitic nematodes from Cyprus and their economic importance. Nematologia Mediterranea, 23 (2): 307-314.
  • Rivoal, R., S. Valette, S. Bekal, J. P. Gauthier & A. Yahyaoui, 2003. Genetic and phenotypic diversity in the graminaceous cyst nematode complex, inferred from PCR-RFLP of ribosomal DNA and morphometric analysis. European Journal of Plant Pathology, 109: 227-241.
  • Rumpenhorst, H. J., I. H. Elekçioğlu, D. Sturhan, G. Öztürk & S. Enneli, 1996. The Cereal Cyst Nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterranea, 24: 135-138.
  • Sahin, E., Nicol, İ. H. Elekcioğlu & R. Rivoal, 2010. Hatching of Heterodera filipjevi in controlled and natural temperature conditions in Turkey. Nematology, 12 (2): 193-200.
  • Saxena, M. E., A. A. EI-Moneim, O. F. Mamluk & S. B. Hanounik, 1988. “A review of nematology research in ICARDA, 69-84”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Sikora, R. A., 1988. “Plant parasitic nematodes of wheat and barley in temperate and semi-arid regions. A comparative analysis, 46-48”. Proceedings of Nematodes Parasitic to Cereals and Legumes in Temperate Semi-Arid Regions Workshop (Eds. M. C. Saxena, R. A. Sikora & J. P. Srivastava) (1-5 March 1987, Larnaca, Cyprus), 217 pp.
  • Smiley, R. W. & J. M. Nicol, 2009. Nematodes which challenge global wheat production. Wheat Science and Trade, 171-187.
  • Smiley, R. W., G. P. Yan & Z. A. Handoo, 2008. First record of the cyst nematode Heterodera filipjevi on wheat in Oregon. Plant Disease, 92 (7): 1136-1136.
  • Sturhan, D. & E. Krall, 2002. Heterodera iri Mathews, 1971 a junior synonym of H. ustinovi Kirjanova, 1969. Russian Journal of Nematology, 10 (1): 55-57.
  • Subbotin, S. A., A. Mundo-Ocampo & J. G. Baldwin, 2010. Systematics of Cyst Nematodes (Nematode: Heteroderinae). Nematology Monographs and Perspectives, 8A. Leiden, the Netherlands, Brill, 364 pp.
  • Subbotin, S. A., D. Sturhan, H. J. Rumpenhorst & M. Moens, 2002. Description of the Australian cereal cyst nematode Heterodera australis sp. n. (Tylenchida: Heteroderidae). Russian Journal of Nematology, 10 (2): 139-148.
  • Subbotin, S. A., D. Sturhan, H. J. Rumpenhorst & M. Moens, 2003. Molecular and morphological characterization of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology, 5: 515-538.
  • Subbotin, S. A., A. Vierstraete, P. De Ley, J. Rowe, L. Waeyenberge, M. Moens & J. R. Vanfleteren, 2001. Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA. Molecular Phylogenetics and Evolution, 21: 1-16.
  • Subbotin, S. A., L. Waeyenberge, I. A. Molokanova & M. Moens, 1999. Identification of Heterodera avenae group species by morphometrics and rDNARFLPs. Nematology, 1: 195-207.
  • Szalanski, A. L., D. D. Sui, T. S. Harris & T. O. Powers, 1997. Identification of cyst nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 29 (3): 255-267.
  • Tamura, K. & M. Nei, 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3): 512-526.
  • Thompson, J. D., D. G. Higgins & T. J. Gibson, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22 (22): 4673-4680.
  • Toktay, H., M. İmren, A. Öcal, L. Waeyenberge, N. Viaene & A. Dababat, 2015. Incidence of cereal cyst nematodes in the East Anatolia Region in Turkey. Russian Journal of Nematology, 23 (1): 29-40.
  • Wouts, W. M. & D. Sturhan, 1995. Heterodera aucklandica sp. n. (Nematoda: Heteroderidae) from a New Zealand native grass, with notes on the species of the H. avenae group. New Zealand Journal of Zoology, 22 (2): 199-207.
There are 43 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Adem Özarslandan 0000-0003-4125-1028

Mustafa İmren 0000-0002-7217-9092

Göksel Özer 0000-0002-3385-2520

Cem Karaca 0000-0003-3824-8290

Abdelfattah Dababat 0000-0002-3172-0452

Publication Date June 1, 2020
Submission Date December 20, 2019
Acceptance Date March 11, 2020
Published in Issue Year 2020 Volume: 44 Issue: 2

Cite

APA Özarslandan, A., İmren, M., Özer, G., Karaca, C., et al. (2020). Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus. Turkish Journal of Entomology, 44(2), 273-281. https://doi.org/10.16970/entoted.661964
AMA Özarslandan A, İmren M, Özer G, Karaca C, Dababat A. Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus. TED. June 2020;44(2):273-281. doi:10.16970/entoted.661964
Chicago Özarslandan, Adem, Mustafa İmren, Göksel Özer, Cem Karaca, and Abdelfattah Dababat. “Identification and Genetic Diversity of the Mediterranean Cereal Cyst Nematode, Heterodera Latipons Franklin, 1969 (Tylenchida: Heteroderidae) in Cereal Production Areas of Northern Cyprus”. Turkish Journal of Entomology 44, no. 2 (June 2020): 273-81. https://doi.org/10.16970/entoted.661964.
EndNote Özarslandan A, İmren M, Özer G, Karaca C, Dababat A (June 1, 2020) Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus. Turkish Journal of Entomology 44 2 273–281.
IEEE A. Özarslandan, M. İmren, G. Özer, C. Karaca, and A. Dababat, “Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus”, TED, vol. 44, no. 2, pp. 273–281, 2020, doi: 10.16970/entoted.661964.
ISNAD Özarslandan, Adem et al. “Identification and Genetic Diversity of the Mediterranean Cereal Cyst Nematode, Heterodera Latipons Franklin, 1969 (Tylenchida: Heteroderidae) in Cereal Production Areas of Northern Cyprus”. Turkish Journal of Entomology 44/2 (June 2020), 273-281. https://doi.org/10.16970/entoted.661964.
JAMA Özarslandan A, İmren M, Özer G, Karaca C, Dababat A. Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus. TED. 2020;44:273–281.
MLA Özarslandan, Adem et al. “Identification and Genetic Diversity of the Mediterranean Cereal Cyst Nematode, Heterodera Latipons Franklin, 1969 (Tylenchida: Heteroderidae) in Cereal Production Areas of Northern Cyprus”. Turkish Journal of Entomology, vol. 44, no. 2, 2020, pp. 273-81, doi:10.16970/entoted.661964.
Vancouver Özarslandan A, İmren M, Özer G, Karaca C, Dababat A. Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus. TED. 2020;44(2):273-81.