Research Article
BibTex RIS Cite

Investigation of Genetic Diversity in Lentil Genotypes Obtain from Different Countries Using SDS-PAGE Methodology

Year 2024, Volume: 17 Issue: 1, 80 - 90, 28.03.2024

Mehmet Zahit Yeken Faheem Baloch Muhammad Azhar Nadeem Muhammad Sameeullah Ekrem Gürel

https://doi.org/10.18185/erzifbed.1323490

Abstract

Baklagiller, tüm dünyada milyonlarca insan için yüksek kaliteli gıda kaynağı sunan tarımsal üretim sisteminin önemli bir parçasıdır. Baklagiller arasında yer alan mercimek, yüksek amino asit, protein ve diyet lifi ve düşük yağ oranına sahip, tek yıllık ve kendi kendine tozlaşan bir bitkidir. Bu çalışmada, 44 mercimek genotipinin karakterizasyonu, SDS-PAGE kullanılarak gerçekleştirilmiştir. Protein bantları, var (1) veya yok (0) şeklinde puanlanmış ve mercimek genotiplerinin bant aralığının 12-20 bant arasında olduğu belirlenmiştir. Jaccard’ın genetik benzerlik katsayısı (GD), ortalama 0.216 olarak tespit edilmiştir. Suriye2 ile Irak3 genotipleri arasında maksimum genetik uzaklık 0.526 olarak bulunmuş ve gelecekteki mercimek ıslahı çalışmalarında kullanılabileceği belirlenmiştir. AMOVA mercimek genotiplerindeki yüksek genetik çeşitliliğini varlığını araştırmış ve populasyon içindeki (83%) varyasyonun populasyonlar arasındaki (17%) varyasyona kıyasla yüksek olduğunu göstermiştir. STRUCTURE algoritması, mercimek genotiplerini coğrafi bölgelerine göre iki gruba ayırmıştır. UPGMA kümelemesi, mercimek genotiplerini iki ana kümeye (A ve B) ayırmıştır. STRUCTURE ve UPGMA analizlerinin sonuçlarını doğrulamak için PCoA gerçekleştirilmiş ve sonuçların uyum içerisinde olduğu belirlenmiştir. Araştırma sonuçları dünyanın farklı yerlerinde mercimek ıslahı ile ilgilenen araştırmacılara önemli katkılar sağlayacaktır.

Keywords

Genetik çeşitlilik germplazm karakterizasyonu populasyon yapısı protein

References

  • [1] Maphosa, Y., Jideani, V.A., (2017) Functional Food. María Chávarri Hueda, The Role of Legumes in Human Nutrition (pp: 1-13) IntechOpen: London, UK.
  • [2] Atnaf, M., Tesfaye, K., Dagne, K., (2015) The importance of legumes in the Ethiopian farming system and overall economy: An overview, American J. Exp. Agric., 7, 347-358.
  • [3] Singh, M., Sharma, S. K., Singh, B., Malhotra, N., Chandora, R., Sarker, A., Singh, K., Gupta, D., (2018) Widening the genetic base of cultivated gene pool following introgression from wild Lens taxa, Plant Breed., 137, 470-485.
  • [4] Ford-Lloyd, B., (2011) Wild Crop Relatives. Genomic and Breeding Resources. Legume Crops and Forages. Experimental Agriculture, 48(1), 152-153. Springer, Heidelberg, Dordrecht, London, New York.
  • [5] Pal, T., Ghosh, S., Mondal, A., De, K. K., (2016) Evaluation of genetic diversity in some promising varieties of lentil using karyological characters and protein profiling, J. Genetic Eng. Biotechnol., 14, 39-48.
  • [6] Dissanayake, R., Braich, S., Cogan, N. O., Smith, K., Kaur, S., (2020) Characterization of genetic and allelic diversity amongst cultivated and wild lentil accessions for germplasm enhancement, Front. Gen., 11, 546.
  • [7] Shah, Z., Shah, S., Peoples, M., Schwenke, G., Herridge, D., (2003) Crop residue and fertiliser N effects on nitrogen fixation and yields of legume–cereal rotations and soil organic fertility, Field Crops Res., 83, 1-11.
  • [8] FAO (2023). Food and Agriculture Statistics. Retrieved October 06, 2023 from: https:// www.fao.org/faostat/en/#home
  • [9] Nadeem, M. A., Yeken, M. Z., Shahid, M. Q., Habyarimana, E., Yılmaz, H., Alsaleh, A., Hatipoğlu, R., Çilesiz, Y., Khawar, K.M., Ludidi, N., (2021) Common bean as a potential crop for future food security: an overview of past, current and future contributions in genomics, transcriptomics, transgenics and proteomics, Biotechnol. Biotechnol. Equip., 35, 759-787.
  • [10] Yeken, M. Z., Emiralioğlu, O., Çiftçi, V., Bayraktar, H., Palacioğlu, G., Özer, G. (2022). Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers. Mol.Biol. Rep., 49(5), 3839-3847.
  • [11] Shah, M. A., Khan, A. I., Awan, F. S., Sadaqat, H. A., Bahadur, S., Baloch, F. S., (2015) Genetic diversity of some tomato cultivars and breeding lines commonly used in Pakistani breeding program, Turk J Agric For., 3, 126-132.
  • [12] Nadeem, M. A., Karaköy, T., Yeken, M. Z., Habyarimana, E., Hatipoğlu, R., Çiftçi, V., Nawaz, M. A., Sönmez, F., Shahid, M. Q., Yang, S. H., (2020) Phenotypic characterization of 183 Turkish common bean accessions for agronomic, trading, and consumer-preferred plant characteristics for breeding purposes, Agronomy., 10, 272.
  • [13] Khazaei, H., Caron, C. T., Fedoruk, M., Diapari, M., Vandenberg, A., Coyne, C.J. McGee, R. Bett, K.E. (2016) Genetic diversity of cultivated lentil (Lens culinaris Medik.) and its relation to the world's agro-ecological zones, Front. Plant Sci., 7, 1093.
  • [14] Ali, A. Altaf, M. T., Nadeem, M. A., Karaköy, T., Shah, A. N., Azeem, H., Baloch, F. S. Baran, N., Hussain, T., (2022) Duangpan, S. Recent advancement in OMICS approaches to enhance abiotic stress tolerance in legumes, Front. Plant Sci., 13, 952759.
  • [15] Havey, M., Muehlbauer, F., (1989) Variability for restriction fragment lengths and phylogenies in lentil, Theo. Appl. Genet., 77, 839-843.
  • [16] Yüzbaşıoğlu, E., Özcan, S., Açık, L., (2006) Analysis of genetic relationships among Turkish cultivars and breeding lines of Lens culinatis Mestile using RAPD markers, Genet. Resou. Crop Evo., 53, 507-514.
  • [17] Alghamdi, S. S., Khan, A. M., Ammar, M. H., El-Harty, E. H., Migdadi, H. M., Abd El-Khalik, S. M., Al-Shameri, A. M., Javed, M. M., Al-Faifi, S. A., (2013) Phenological, nutritional and molecular diversity assessment among 35 introduced lentil (Lens culinaris Medik.) genotypes grown in Saudi Arabia, Inter. J. Mol Sci., 15, 277-295.
  • [18] Seyedimoradi, H., Talebi, R., (2014) Detecting DNA polymorphism and genetic diversity in Lentil (Lens culinaris Medik.) germplasm: comparison of ISSR and DAMD marker, Physiol. Mol. Biol. Plant., 20, 495-500.
  • [19] Tsanakas, G. F., Mylona, P. V., Koura, K., Gleridou, A., Polidoros, A. N., (2018) Genetic diversity analysis of the Greek lentil (Lens culinaris) landrace ‘Eglouvis’ using morphological and molecular markers, Plant Gen. Res., 16, 469-477.
  • [20] Duygu, A., (2019) Genetic diversity in lentil landraces revealed by diversity array technology (DArT), Turkish J. Field Crop., 24, 252-260.
  • [21] Lombardi, M., Materne, M., Cogan, N. O., Rodda, M., Daetwyler, H. D., Slater, A. T., Forster, J. W., Kaur, S., (2014) Assessment of genetic variation within a global collection of lentil (Lens culinaris Medik.) cultivars and landraces using SNP markers, BMC Gen., 15, 1-10.
  • [22] Wong, M. M., Gujaria-Verma, N., Ramsay, L., Yuan, H. Y., Caron, C., Diapari, M., Vandenberg, A., Bett, K. E., (2015) Classification and characterization of species within the genus Lens using genotyping-by-sequencing (GBS), PLoS One., 10, e0122025.
  • [23] Jan, S. A., Shinwari, Z. K., Rabbani, M. A., Shah, S. H., Ibrahim, M. I., Ilyas, M., (2016) Optimization of an efficient SDS-PAGE protocol for rapid protein analysis of Brassica rapa., J. Bio. Env. Sci., 9, 17-24.
  • [24] Kakaei, M., Kahrizi, D., (2011) Study of seed proteins pattern of Brassica napus varieties via sodium dodecyl sulfate polyacrylamid gel electrophoresis, Inter. Res. J. Biotech., 2, 026-028.
  • [25] Javaid, A., Ghafoor, A., Anwar, R., (2004) Seed storage protein electrophoresis in groundnut for evaluating genetic diversity, Pak. J. Bot., 36, 25-30.
  • [26] Berber, İ., Yaşar, F., (2011) Characterization of Bean (Phaseolus vulgaris L.) cultivars grown in Turkey by SDS-PAGE of seed proteins, Pak. J. Bot., 43, 1085-1090.
  • [27] Sher, K., Nisar, M., Subhan, M., Hazrat, A., Muhammad, A., Hancı, F., Fazal, Z., (2020) Protein Profiling of Indegnous Common Bean Through SDS-PAGE, Biosci. Res., 17, 688-898.
  • [28] Yaldiz, G., Sameeullah, M., Çamlıca, M., Baloch, F. S., (2016) Lack of population structure in coriander populations based on SDS (Seed Storage Protein) page analysis, Turkish J.Agric. Food Sci.Tech., 4, 656-661.
  • [29] Zaccardelli, M., Lupo, F., Piergiovanni, A. R., Laghetti, G., Sonnante, G., Daminati, M. G., Sparvoli, F., Lioi, L., (2012) Characterization of Italian lentil (Lens culinaris Medik.) germplasm by agronomic traits, biochemical and molecular markers, Genet. Resou. Crop Evo., 59, 727-738.
  • [30] Kumar, P., Trivedi, A., Adarsh, A., Verma, R., Kumar, R., Vyas, R., Yadav, M., (2018) Genetic characterization of lentil genotypes based on SDS-PAGE, J. Pharm. Phytochem., 7, 274-278.
  • [31] Ghafoor, A., Ahmad, Z., Qureshi, A. S., Bashir, M., (2002) Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE, Euphytica., 123, 367-388.
  • [32] Sameeullah, M., Sasaki, T., Yamamoto, Y., (2013) Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.), Soil Sci. Plant Nut., 59, 756-770.
  • [33] Laemmli, U. K., (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature., 227, 680-685.
  • [34] Jaccard, P., (1908) Nouvelles recherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44, 223-270.
  • [35] Evanno, G., Regnaut, S., Goudet, J., (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study, Mol. Ecol., 14, 2611-2620.
  • [36] Scippa, G. S., Rocco, M., Ialicicco, M., Trupiano, D., Viscosi, V., Di Michele, M., Arena, S., Chiatante, D., Scaloni, A., (2010) The proteome of lentil (Lens culinaris Medik.) seeds: discriminating between landraces, Electrophoresis., 31, 497-506.
  • [37] Fikiru, E., Tesfaye, K., Bekele, E., (2007) Genetic diversity and population structure of Ethiopian lentil (Lens culinaris Medikus) landraces as revealed by ISSR marker, African J. Biotechnol., 6, 1460-1468.
  • [38] Yüzbaşioğlu, E., Acik, L., Özcan, S., (2008) Seed protein diversity among lentil cultivars, Biologia Plantarum., 52, 126-128.
  • [39] Toklu, F., Karaköy, T., Haklı, E., Bicer, T., Brandolini, A., Kilian, B., Özkan, H., (2009) Genetic variation among lentil (Lens culinaris Medik) landraces from Southeast Turkey, Plant Breed., 128, 178-186.
  • [40] Kushwaha, U., Ghimire, S., Yadav, N., Ojha, B., (2013) Genetic relatedness of lentil (Lens culinaris L.) germplasm by using SSR markers, Int. J. Appl. Sci. Biotechnol., 1, 132-136.
  • [41] Tahir, N. A. R., Omer, D., (2017) Genetic variation in lentil genotypes by morpho-agronomic traits and RAPD-PCR, J Anim. Plant Sci. 27, 468-480.

Investigation of genetic diversity in lentil genotypes obtain from different countries using SDS-PAGE methodology

Year 2024, Volume: 17 Issue: 1, 80 - 90, 28.03.2024

Mehmet Zahit Yeken Faheem Baloch Muhammad Azhar Nadeem Muhammad Sameeullah Ekrem Gürel

https://doi.org/10.18185/erzifbed.1323490

Abstract

Pulses are an important component of the agricultural production system, providing high-quality food to millions of people worldwide. Among pulses, lentil is an annual and self-pollinated crop known to be its high proteins, amino acids, dietary fibers and low fat in the world. In the current study, the characterization of 44 lentil genotypes was performed using SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) technique. The protein bands were scored according to a binary system as the present (1) or absent (0) that ranged 12-20 bands in lentil genotypes. The Jaccard’s coefficient of genetic dissimilarity (GD) was measured, and the mean GD was 0.216. Maximum genetic distance was found as 0.526 between Syria2 with Iraq3 and these genotypes might be recommended for future lentil breeding. AMOVA explored the presence of higher genetic variety within genotypes (83%) than among genotypes (17% variations). STRUCTURE algorithm separated lentil genotypes into two groups mainly on the basis of their geographic. The UPGMA clustering separated lentil genotypes into two main clusters A and B. The PCoA was also conducted to confirm the results of structure and UPGMA analyses. Findigs from the PCoA and structure analyses were in full agreement with those obtained by UPGMA. The results might be useful for researchers worldwide who are interested in lentil breeding.

Keywords

Genetic diversity germplasm characterization population structure

References

  • [1] Maphosa, Y., Jideani, V.A., (2017) Functional Food. María Chávarri Hueda, The Role of Legumes in Human Nutrition (pp: 1-13) IntechOpen: London, UK.
  • [2] Atnaf, M., Tesfaye, K., Dagne, K., (2015) The importance of legumes in the Ethiopian farming system and overall economy: An overview, American J. Exp. Agric., 7, 347-358.
  • [3] Singh, M., Sharma, S. K., Singh, B., Malhotra, N., Chandora, R., Sarker, A., Singh, K., Gupta, D., (2018) Widening the genetic base of cultivated gene pool following introgression from wild Lens taxa, Plant Breed., 137, 470-485.
  • [4] Ford-Lloyd, B., (2011) Wild Crop Relatives. Genomic and Breeding Resources. Legume Crops and Forages. Experimental Agriculture, 48(1), 152-153. Springer, Heidelberg, Dordrecht, London, New York.
  • [5] Pal, T., Ghosh, S., Mondal, A., De, K. K., (2016) Evaluation of genetic diversity in some promising varieties of lentil using karyological characters and protein profiling, J. Genetic Eng. Biotechnol., 14, 39-48.
  • [6] Dissanayake, R., Braich, S., Cogan, N. O., Smith, K., Kaur, S., (2020) Characterization of genetic and allelic diversity amongst cultivated and wild lentil accessions for germplasm enhancement, Front. Gen., 11, 546.
  • [7] Shah, Z., Shah, S., Peoples, M., Schwenke, G., Herridge, D., (2003) Crop residue and fertiliser N effects on nitrogen fixation and yields of legume–cereal rotations and soil organic fertility, Field Crops Res., 83, 1-11.
  • [8] FAO (2023). Food and Agriculture Statistics. Retrieved October 06, 2023 from: https:// www.fao.org/faostat/en/#home
  • [9] Nadeem, M. A., Yeken, M. Z., Shahid, M. Q., Habyarimana, E., Yılmaz, H., Alsaleh, A., Hatipoğlu, R., Çilesiz, Y., Khawar, K.M., Ludidi, N., (2021) Common bean as a potential crop for future food security: an overview of past, current and future contributions in genomics, transcriptomics, transgenics and proteomics, Biotechnol. Biotechnol. Equip., 35, 759-787.
  • [10] Yeken, M. Z., Emiralioğlu, O., Çiftçi, V., Bayraktar, H., Palacioğlu, G., Özer, G. (2022). Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers. Mol.Biol. Rep., 49(5), 3839-3847.
  • [11] Shah, M. A., Khan, A. I., Awan, F. S., Sadaqat, H. A., Bahadur, S., Baloch, F. S., (2015) Genetic diversity of some tomato cultivars and breeding lines commonly used in Pakistani breeding program, Turk J Agric For., 3, 126-132.
  • [12] Nadeem, M. A., Karaköy, T., Yeken, M. Z., Habyarimana, E., Hatipoğlu, R., Çiftçi, V., Nawaz, M. A., Sönmez, F., Shahid, M. Q., Yang, S. H., (2020) Phenotypic characterization of 183 Turkish common bean accessions for agronomic, trading, and consumer-preferred plant characteristics for breeding purposes, Agronomy., 10, 272.
  • [13] Khazaei, H., Caron, C. T., Fedoruk, M., Diapari, M., Vandenberg, A., Coyne, C.J. McGee, R. Bett, K.E. (2016) Genetic diversity of cultivated lentil (Lens culinaris Medik.) and its relation to the world's agro-ecological zones, Front. Plant Sci., 7, 1093.
  • [14] Ali, A. Altaf, M. T., Nadeem, M. A., Karaköy, T., Shah, A. N., Azeem, H., Baloch, F. S. Baran, N., Hussain, T., (2022) Duangpan, S. Recent advancement in OMICS approaches to enhance abiotic stress tolerance in legumes, Front. Plant Sci., 13, 952759.
  • [15] Havey, M., Muehlbauer, F., (1989) Variability for restriction fragment lengths and phylogenies in lentil, Theo. Appl. Genet., 77, 839-843.
  • [16] Yüzbaşıoğlu, E., Özcan, S., Açık, L., (2006) Analysis of genetic relationships among Turkish cultivars and breeding lines of Lens culinatis Mestile using RAPD markers, Genet. Resou. Crop Evo., 53, 507-514.
  • [17] Alghamdi, S. S., Khan, A. M., Ammar, M. H., El-Harty, E. H., Migdadi, H. M., Abd El-Khalik, S. M., Al-Shameri, A. M., Javed, M. M., Al-Faifi, S. A., (2013) Phenological, nutritional and molecular diversity assessment among 35 introduced lentil (Lens culinaris Medik.) genotypes grown in Saudi Arabia, Inter. J. Mol Sci., 15, 277-295.
  • [18] Seyedimoradi, H., Talebi, R., (2014) Detecting DNA polymorphism and genetic diversity in Lentil (Lens culinaris Medik.) germplasm: comparison of ISSR and DAMD marker, Physiol. Mol. Biol. Plant., 20, 495-500.
  • [19] Tsanakas, G. F., Mylona, P. V., Koura, K., Gleridou, A., Polidoros, A. N., (2018) Genetic diversity analysis of the Greek lentil (Lens culinaris) landrace ‘Eglouvis’ using morphological and molecular markers, Plant Gen. Res., 16, 469-477.
  • [20] Duygu, A., (2019) Genetic diversity in lentil landraces revealed by diversity array technology (DArT), Turkish J. Field Crop., 24, 252-260.
  • [21] Lombardi, M., Materne, M., Cogan, N. O., Rodda, M., Daetwyler, H. D., Slater, A. T., Forster, J. W., Kaur, S., (2014) Assessment of genetic variation within a global collection of lentil (Lens culinaris Medik.) cultivars and landraces using SNP markers, BMC Gen., 15, 1-10.
  • [22] Wong, M. M., Gujaria-Verma, N., Ramsay, L., Yuan, H. Y., Caron, C., Diapari, M., Vandenberg, A., Bett, K. E., (2015) Classification and characterization of species within the genus Lens using genotyping-by-sequencing (GBS), PLoS One., 10, e0122025.
  • [23] Jan, S. A., Shinwari, Z. K., Rabbani, M. A., Shah, S. H., Ibrahim, M. I., Ilyas, M., (2016) Optimization of an efficient SDS-PAGE protocol for rapid protein analysis of Brassica rapa., J. Bio. Env. Sci., 9, 17-24.
  • [24] Kakaei, M., Kahrizi, D., (2011) Study of seed proteins pattern of Brassica napus varieties via sodium dodecyl sulfate polyacrylamid gel electrophoresis, Inter. Res. J. Biotech., 2, 026-028.
  • [25] Javaid, A., Ghafoor, A., Anwar, R., (2004) Seed storage protein electrophoresis in groundnut for evaluating genetic diversity, Pak. J. Bot., 36, 25-30.
  • [26] Berber, İ., Yaşar, F., (2011) Characterization of Bean (Phaseolus vulgaris L.) cultivars grown in Turkey by SDS-PAGE of seed proteins, Pak. J. Bot., 43, 1085-1090.
  • [27] Sher, K., Nisar, M., Subhan, M., Hazrat, A., Muhammad, A., Hancı, F., Fazal, Z., (2020) Protein Profiling of Indegnous Common Bean Through SDS-PAGE, Biosci. Res., 17, 688-898.
  • [28] Yaldiz, G., Sameeullah, M., Çamlıca, M., Baloch, F. S., (2016) Lack of population structure in coriander populations based on SDS (Seed Storage Protein) page analysis, Turkish J.Agric. Food Sci.Tech., 4, 656-661.
  • [29] Zaccardelli, M., Lupo, F., Piergiovanni, A. R., Laghetti, G., Sonnante, G., Daminati, M. G., Sparvoli, F., Lioi, L., (2012) Characterization of Italian lentil (Lens culinaris Medik.) germplasm by agronomic traits, biochemical and molecular markers, Genet. Resou. Crop Evo., 59, 727-738.
  • [30] Kumar, P., Trivedi, A., Adarsh, A., Verma, R., Kumar, R., Vyas, R., Yadav, M., (2018) Genetic characterization of lentil genotypes based on SDS-PAGE, J. Pharm. Phytochem., 7, 274-278.
  • [31] Ghafoor, A., Ahmad, Z., Qureshi, A. S., Bashir, M., (2002) Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE, Euphytica., 123, 367-388.
  • [32] Sameeullah, M., Sasaki, T., Yamamoto, Y., (2013) Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacum L.), Soil Sci. Plant Nut., 59, 756-770.
  • [33] Laemmli, U. K., (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature., 227, 680-685.
  • [34] Jaccard, P., (1908) Nouvelles recherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44, 223-270.
  • [35] Evanno, G., Regnaut, S., Goudet, J., (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study, Mol. Ecol., 14, 2611-2620.
  • [36] Scippa, G. S., Rocco, M., Ialicicco, M., Trupiano, D., Viscosi, V., Di Michele, M., Arena, S., Chiatante, D., Scaloni, A., (2010) The proteome of lentil (Lens culinaris Medik.) seeds: discriminating between landraces, Electrophoresis., 31, 497-506.
  • [37] Fikiru, E., Tesfaye, K., Bekele, E., (2007) Genetic diversity and population structure of Ethiopian lentil (Lens culinaris Medikus) landraces as revealed by ISSR marker, African J. Biotechnol., 6, 1460-1468.
  • [38] Yüzbaşioğlu, E., Acik, L., Özcan, S., (2008) Seed protein diversity among lentil cultivars, Biologia Plantarum., 52, 126-128.
  • [39] Toklu, F., Karaköy, T., Haklı, E., Bicer, T., Brandolini, A., Kilian, B., Özkan, H., (2009) Genetic variation among lentil (Lens culinaris Medik) landraces from Southeast Turkey, Plant Breed., 128, 178-186.
  • [40] Kushwaha, U., Ghimire, S., Yadav, N., Ojha, B., (2013) Genetic relatedness of lentil (Lens culinaris L.) germplasm by using SSR markers, Int. J. Appl. Sci. Biotechnol., 1, 132-136.
  • [41] Tahir, N. A. R., Omer, D., (2017) Genetic variation in lentil genotypes by morpho-agronomic traits and RAPD-PCR, J Anim. Plant Sci. 27, 468-480.
There are 41 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology
Journal Section Makaleler
Authors

Mehmet Zahit Yeken 0000-0003-0490-371X

Faheem Baloch 0000-0002-7470-0080

Muhammad Azhar Nadeem 0000-0002-0637-9619

Muhammad Sameeullah 0000-0001-6767-2539

Ekrem Gürel 0000-0001-6262-2866

Early Pub Date March 27, 2024
Publication Date March 28, 2024
Published in Issue Year 2024 Volume: 17 Issue: 1

Cite

APA Yeken, M. Z., Baloch, F., Nadeem, M. A., Sameeullah, M., et al. (2024). Investigation of genetic diversity in lentil genotypes obtain from different countries using SDS-PAGE methodology. Erzincan University Journal of Science and Technology, 17(1), 80-90. https://doi.org/10.18185/erzifbed.1323490
 Download Cover Image