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Avokado (Persea americana Mill.) Genotiplerinin SSR Markörleri İle Moleküler Karakterizasyonu

Year 2019, Volume: 2 Issue: 2, 1 - 6, 24.07.2019

Abstract

Bu çalışmada
moleküler markörlerden SSR tekniği kullanılarak avokado genotipleri arasındaki farklılıkların
ortaya konulması amaçlanmıştır. Avokadonun Lauraceae
familyasına ait Persea cinsi
içerisinde yer aldığı bilinmektedir. Çalışma kapsamında Akdeniz Bölgesinde
yetiştirilen Antalya ilinin dahil olduğu Batı Akdeniz Tarımsal Araştırma
Enstitüsü’nden 5 ve Japonya’dan getirtilen 7 çeşit avokado moleküler analizler
için kullanılmıştır. Bu amaçla SSR markörleri ile yapılan analizler sonucunda
avokado çeşitleri arasında iki ana grup ortaya çıkmış ve % 60 oranında
benzerlik gösterdikleri belirlenmiştir. Çalışmada kullanılan çeşitlerden JP4 ile
JP6 ve JP5 ile JP7 arasında yakın korelasyon olduğu gözlemlenmiştir. Zutano ve
Hass çeşitlerini birbirlerinden ayırt edecek polimorfizmler üretilememiş ve bu
iki çeşit bir arada gruplanmıştır. JP1, JP2, JP3 ve Bacon çeşitleri tek başına
bir alt grup oluşturmuştur. Toplam allel sayısının 152, spesifik allel sayısının
53 adet olduğu ve bant büyüklüğünün ise 179 ile 283 bç arasında değiştiği belirlenmiştir.
Polimorfik bilgi içeriği (PBİ) 0,03 ile 0,85 arasında değişim göstermiştir.  Çalışma sonucunda elde edilen bulgular, yapılacak
ıslah çalışmalarında ebeveyn seçiminde bir basamak oluşturabileceği gibi,
avokado genotiplerinin yayılma alanlarının belirlenmesinde, genetik koleksiyonların
karşılaştırılmasında ve avokado genotiplerinin karakterizasyonunda
kullanılabilir.

References

  • Acheampong, A.K., Akromah, R., and Ofori, F.A., 2008. Genetic Characterization of Ghanaian Avocados Using Microsatellite Markers. J. Amer. Soc. Hort. Sci. 133(6): 801-809.
  • Andersen, J. R., and Lübberstedt, T., 2003. Functional markers in plants. Trends in plant science 8(11): 554-560.of Some Pomegranate Cultivars (Punica granatum L.) Growing in Southern Anatolia Region in Turkey. Biochemical Systematics and Ecology 35(11): 764-769. FAOSTAT, 2017. Statistical Database of the Food and Agriculture Organization of the United Nations. Erişim Tarihi:21.10.2018.http://faostat.fao.org/.Fathi, A., Ghareyazi, B., Haghnazari, A., Ghaffari, M. R., Pirseyedi, S. M., Kadkhodaei, S., ... & Mardi, M. (2008). Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits. Iranian Journal of Biotechnology 6(2): 98-106.Gross-German, E., Viruel, 2013. M.A., Molecular Characterization of Avocado Germplasm with a New set of SSR and EST-SSR Markers: Genetic Diversity, Population Structure, and İdentification of Race-Specific Markers in a Group of Cultivated Genotypes. Spain 9: 539-555.Gulen, H., Ipek, A., Ergin, S., Akcay, M.A., 2010. Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers. Journal of Horticultural Science & Biotechnology 85 (5): 427–431. Kafkas, S., Ozgen M., Dogan, Y., Ozcan, B., Ercisli, S., and Serce, S., 2008. Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers. Journal of the American Society for Horticultural Science, 593–597. Minch, E., Ruiz-Linares, A., Goldstein, D., Feldman, M., & Cavalli-Sforza, L. L. (1995). Microsat (version 1.4 d): a computer program for calculating various statistics on microsatellite allele data. WWW: http://hpgl. stanford. edu/projects/microsat/.Mnejja, M., Garcia-Mas, 2005. Development and Transportability Across Prunus Species of 42 Polymorphic Almond Microsatellites. Molecular Ecology Notes. Spain: 531–535.Pınar, H., Unlu M., Ercisli, S., Uzun, A., Bircan, M., Yılmaz, K.U., Agar, G., 2013. Determination of genetic diversity among wild grown apricots from Sakit valley in Turkey using SRAP markers. Journal of Applied Botany and Food Quality 86: 55-58. TÜİK, 2017. Bitkisel Üretim İstatistikleri. http://tuikapp.tuik.gov.tr/bitkiselapp/bitkisel.zul. Erişim Tarihi:18.10.2017Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D., 2010. Bitki Islahında Moleküler Belirteçlerin Kullanımı ve Gen Aktarımı. Türkiye Hijyen ve Deneysel Biyoloji Dergisi 67:(1) 33-43.Wagner, H. W., & Sefc, K. M., 1999. IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, Vienna, 500.Weising, K., Beyermann, B., Ramser, J., and Kahl, G., 1991. Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12(2‐3): 159-169.Yılmaz, KU., Yanar, M., Ercisli, S., Sahiner, H., Taskin, T. and Zengin, Y., 2010. Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes. Biochemical Genetics 48 (9-10): 873-878.
  • Alcaraz, M. L., and Hormaza, J. I., 2007. Molecular Characterization and Genetic Diversity in an Avocado Collection of Cultivars and Local Spanish Genotypes Using SSRs. ̶Hereditas 144: 244 -253.
  • Anonim, 2005. Tarımsal Yapı-Üretim, Fiyat, Değer. TÜİK. Anonim, 2007. Agricultural Statistical Database. Erişim Tarihi:19.10.2017. http://www.fao.org.
  • Weising, K., Beyermann, B., Ramser, J., and Kahl, G., 1991. Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12(2‐3): 159-169.
  • Bower, J. P., and J. G. Cutting. 1988. Avocado Fruit Development and Ripening Physiology. In: J. Janick (Editör) Horticultural Reviews 10: 229– 271.
  • Çalışkan, M., 2005. RAPD Analizi İle Güllerde Genetik Tanımlama. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, 81s, Ankara.
  • Demirkol, A., 1998. Avocado Growing in Turkey. World Avocado Congress III, 22–27 October 1995, Tel-Aviv, Israel, pp. 451–456.
  • Dirlewanger, E., Cosson, P., Tavaud, M., Aranzana, MJ., Poizat, C., Zanetto, A., Arus, P., Laigret, F., 2002. Development of Microsatellite Markers in Peach [Prunus persica (L.) Batsch] and Their Use İn Genetic Diversity Analysis İn Peach and Sweet Cherry (Prunus avium L.). Theor Appl Genet. 105: 127-138.
  • Doğrular, H.A., Tuncay, M., ve Şengüler, A., 1983. Antalya ve Alanya Koşullarında Avokado Çeşitlerinin Adaptasyonu. (Ara sonuç raporu), Yayınlanmamış, Turunçgiller Araştırma Enstitüsü, Antalya.
  • Ercisli, S., Agar, G., Orhan, E., Yıldırım, N., Hizarci, Y., 2007. Interspecific Variability of RAPD and Fatty Acid Composition of Some Pomegranate Cultivars (Punica granatum L.) Growing in Southern Anatolia Region in Turkey. Biochemical Systematics and Ecology 35(11): 764-769.
  • FAOSTAT, 2017. Statistical Database of the Food and Agriculture Organization of the United Nations. Erişim Tarihi:21.10.2018.http://faostat.fao.org/.
  • Fathi, A., Ghareyazi, B., Haghnazari, A., Ghaffari, M. R., Pirseyedi, S. M., Kadkhodaei, S., ... & Mardi, M. (2008). Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits. Iranian Journal of Biotechnology 6(2): 98-106.
  • Gross-German, E., Viruel, 2013. M.A., Molecular Characterization of Avocado Germplasm with a New set of SSR and EST-SSR Markers: Genetic Diversity, Population Structure, and İdentification of Race-Specific Markers in a Group of Cultivated Genotypes. Spain 9: 539-555.
  • Gulen, H., Ipek, A., Ergin, S., Akcay, M.A., 2010. Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers. Journal of Horticultural Science & Biotechnology 85 (5): 427–431.
  • Kafkas, S., Ozgen M., Dogan, Y., Ozcan, B., Ercisli, S., and Serce, S., 2008. Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers. Journal of the American Society for Horticultural Science, 593–597.
  • Minch, E., Ruiz-Linares, A., Goldstein, D., Feldman, M., & Cavalli-Sforza, L. L. (1995). Microsat (version 1.4 d): a computer program for calculating various statistics on microsatellite allele data. WWW: http://hpgl. stanford. edu/projects/microsat/.
  • Mnejja, M., Garcia-Mas, 2005. Development and Transportability Across Prunus Species of 42 Polymorphic Almond Microsatellites. Molecular Ecology Notes. Spain: 531–535.
  • Pınar, H., Unlu M., Ercisli, S., Uzun, A., Bircan, M., Yılmaz, K.U., Agar, G., 2013. Determination of genetic diversity among wild grown apricots from Sakit valley in Turkey using SRAP markers. Journal of Applied Botany and Food Quality 86: 55-58.
  • TÜİK, 2017. Bitkisel Üretim İstatistikleri. http://tuikapp.tuik.gov.tr/bitkiselapp/bitkisel.zul. Erişim Tarihi:18.10.2017
  • Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D., 2010. Bitki Islahında Moleküler Belirteçlerin Kullanımı ve Gen Aktarımı. Türkiye Hijyen ve Deneysel Biyoloji Dergisi 67:(1) 33-43.
  • Wagner, H. W., & Sefc, K. M., 1999. IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, Vienna, 500.
  • Yılmaz, KU., Yanar, M., Ercisli, S., Sahiner, H., Taskin, T. and Zengin, Y., 2010. Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes. Biochemical Genetics 48 (9-10): 873-878.
Year 2019, Volume: 2 Issue: 2, 1 - 6, 24.07.2019

Abstract

References

  • Acheampong, A.K., Akromah, R., and Ofori, F.A., 2008. Genetic Characterization of Ghanaian Avocados Using Microsatellite Markers. J. Amer. Soc. Hort. Sci. 133(6): 801-809.
  • Andersen, J. R., and Lübberstedt, T., 2003. Functional markers in plants. Trends in plant science 8(11): 554-560.of Some Pomegranate Cultivars (Punica granatum L.) Growing in Southern Anatolia Region in Turkey. Biochemical Systematics and Ecology 35(11): 764-769. FAOSTAT, 2017. Statistical Database of the Food and Agriculture Organization of the United Nations. Erişim Tarihi:21.10.2018.http://faostat.fao.org/.Fathi, A., Ghareyazi, B., Haghnazari, A., Ghaffari, M. R., Pirseyedi, S. M., Kadkhodaei, S., ... & Mardi, M. (2008). Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits. Iranian Journal of Biotechnology 6(2): 98-106.Gross-German, E., Viruel, 2013. M.A., Molecular Characterization of Avocado Germplasm with a New set of SSR and EST-SSR Markers: Genetic Diversity, Population Structure, and İdentification of Race-Specific Markers in a Group of Cultivated Genotypes. Spain 9: 539-555.Gulen, H., Ipek, A., Ergin, S., Akcay, M.A., 2010. Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers. Journal of Horticultural Science & Biotechnology 85 (5): 427–431. Kafkas, S., Ozgen M., Dogan, Y., Ozcan, B., Ercisli, S., and Serce, S., 2008. Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers. Journal of the American Society for Horticultural Science, 593–597. Minch, E., Ruiz-Linares, A., Goldstein, D., Feldman, M., & Cavalli-Sforza, L. L. (1995). Microsat (version 1.4 d): a computer program for calculating various statistics on microsatellite allele data. WWW: http://hpgl. stanford. edu/projects/microsat/.Mnejja, M., Garcia-Mas, 2005. Development and Transportability Across Prunus Species of 42 Polymorphic Almond Microsatellites. Molecular Ecology Notes. Spain: 531–535.Pınar, H., Unlu M., Ercisli, S., Uzun, A., Bircan, M., Yılmaz, K.U., Agar, G., 2013. Determination of genetic diversity among wild grown apricots from Sakit valley in Turkey using SRAP markers. Journal of Applied Botany and Food Quality 86: 55-58. TÜİK, 2017. Bitkisel Üretim İstatistikleri. http://tuikapp.tuik.gov.tr/bitkiselapp/bitkisel.zul. Erişim Tarihi:18.10.2017Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D., 2010. Bitki Islahında Moleküler Belirteçlerin Kullanımı ve Gen Aktarımı. Türkiye Hijyen ve Deneysel Biyoloji Dergisi 67:(1) 33-43.Wagner, H. W., & Sefc, K. M., 1999. IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, Vienna, 500.Weising, K., Beyermann, B., Ramser, J., and Kahl, G., 1991. Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12(2‐3): 159-169.Yılmaz, KU., Yanar, M., Ercisli, S., Sahiner, H., Taskin, T. and Zengin, Y., 2010. Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes. Biochemical Genetics 48 (9-10): 873-878.
  • Alcaraz, M. L., and Hormaza, J. I., 2007. Molecular Characterization and Genetic Diversity in an Avocado Collection of Cultivars and Local Spanish Genotypes Using SSRs. ̶Hereditas 144: 244 -253.
  • Anonim, 2005. Tarımsal Yapı-Üretim, Fiyat, Değer. TÜİK. Anonim, 2007. Agricultural Statistical Database. Erişim Tarihi:19.10.2017. http://www.fao.org.
  • Weising, K., Beyermann, B., Ramser, J., and Kahl, G., 1991. Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12(2‐3): 159-169.
  • Bower, J. P., and J. G. Cutting. 1988. Avocado Fruit Development and Ripening Physiology. In: J. Janick (Editör) Horticultural Reviews 10: 229– 271.
  • Çalışkan, M., 2005. RAPD Analizi İle Güllerde Genetik Tanımlama. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, 81s, Ankara.
  • Demirkol, A., 1998. Avocado Growing in Turkey. World Avocado Congress III, 22–27 October 1995, Tel-Aviv, Israel, pp. 451–456.
  • Dirlewanger, E., Cosson, P., Tavaud, M., Aranzana, MJ., Poizat, C., Zanetto, A., Arus, P., Laigret, F., 2002. Development of Microsatellite Markers in Peach [Prunus persica (L.) Batsch] and Their Use İn Genetic Diversity Analysis İn Peach and Sweet Cherry (Prunus avium L.). Theor Appl Genet. 105: 127-138.
  • Doğrular, H.A., Tuncay, M., ve Şengüler, A., 1983. Antalya ve Alanya Koşullarında Avokado Çeşitlerinin Adaptasyonu. (Ara sonuç raporu), Yayınlanmamış, Turunçgiller Araştırma Enstitüsü, Antalya.
  • Ercisli, S., Agar, G., Orhan, E., Yıldırım, N., Hizarci, Y., 2007. Interspecific Variability of RAPD and Fatty Acid Composition of Some Pomegranate Cultivars (Punica granatum L.) Growing in Southern Anatolia Region in Turkey. Biochemical Systematics and Ecology 35(11): 764-769.
  • FAOSTAT, 2017. Statistical Database of the Food and Agriculture Organization of the United Nations. Erişim Tarihi:21.10.2018.http://faostat.fao.org/.
  • Fathi, A., Ghareyazi, B., Haghnazari, A., Ghaffari, M. R., Pirseyedi, S. M., Kadkhodaei, S., ... & Mardi, M. (2008). Assessment of the genetic diversity of almond (Prunus dulcis) using microsatellite markers and morphological traits. Iranian Journal of Biotechnology 6(2): 98-106.
  • Gross-German, E., Viruel, 2013. M.A., Molecular Characterization of Avocado Germplasm with a New set of SSR and EST-SSR Markers: Genetic Diversity, Population Structure, and İdentification of Race-Specific Markers in a Group of Cultivated Genotypes. Spain 9: 539-555.
  • Gulen, H., Ipek, A., Ergin, S., Akcay, M.A., 2010. Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers. Journal of Horticultural Science & Biotechnology 85 (5): 427–431.
  • Kafkas, S., Ozgen M., Dogan, Y., Ozcan, B., Ercisli, S., and Serce, S., 2008. Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers. Journal of the American Society for Horticultural Science, 593–597.
  • Minch, E., Ruiz-Linares, A., Goldstein, D., Feldman, M., & Cavalli-Sforza, L. L. (1995). Microsat (version 1.4 d): a computer program for calculating various statistics on microsatellite allele data. WWW: http://hpgl. stanford. edu/projects/microsat/.
  • Mnejja, M., Garcia-Mas, 2005. Development and Transportability Across Prunus Species of 42 Polymorphic Almond Microsatellites. Molecular Ecology Notes. Spain: 531–535.
  • Pınar, H., Unlu M., Ercisli, S., Uzun, A., Bircan, M., Yılmaz, K.U., Agar, G., 2013. Determination of genetic diversity among wild grown apricots from Sakit valley in Turkey using SRAP markers. Journal of Applied Botany and Food Quality 86: 55-58.
  • TÜİK, 2017. Bitkisel Üretim İstatistikleri. http://tuikapp.tuik.gov.tr/bitkiselapp/bitkisel.zul. Erişim Tarihi:18.10.2017
  • Vardar-Kanlıtepe, Ç., Aras, S., Cansaran-Duman, D., 2010. Bitki Islahında Moleküler Belirteçlerin Kullanımı ve Gen Aktarımı. Türkiye Hijyen ve Deneysel Biyoloji Dergisi 67:(1) 33-43.
  • Wagner, H. W., & Sefc, K. M., 1999. IDENTITY 1.0. Centre for Applied Genetics, University of Agricultural Sciences, Vienna, 500.
  • Yılmaz, KU., Yanar, M., Ercisli, S., Sahiner, H., Taskin, T. and Zengin, Y., 2010. Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes. Biochemical Genetics 48 (9-10): 873-878.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Yaşar Karakurt

Müge Özeren This is me

Damla Güvercin

Publication Date July 24, 2019
Acceptance Date May 8, 2019
Published in Issue Year 2019 Volume: 2 Issue: 2

Cite

APA Karakurt, Y., Özeren, M., & Güvercin, D. (2019). Avokado (Persea americana Mill.) Genotiplerinin SSR Markörleri İle Moleküler Karakterizasyonu. Erciyes Tarım Ve Hayvan Bilimleri Dergisi, 2(2), 1-6.