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Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids

Year 2011, Volume: 39 Issue: 2, 157 - 162, 01.04.2011

Zafer Ömer Özdemir Murat Şentürk Deniz Ekinci

Abstract

The aim of this study was to assess activatory potentials of 4’- Methylsulfonyl - acetophenone, 4- methylsulfonyl phenylacetic acid, tyrosine and phenylalanine on human erythrocyte glucose 6-phosphate dehydrogenase. For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was managed to be purified 812-fold in a yield of 40.6% by using 2’,5’-ADP Sepharose 4B affinity gel. 4’- Methylsulfonyl acetophenone, 4- methylsulfonyl phenylacetic acid, tyrosine and phenylalanine exhibited activatory effects on the enzyme. % Activity values for these compounds were determined by plotting activity percentage versus [Activator].

Keywords

Activator amino acid enzyme glucose 6-phosphate dehydrogenase

References

  • W. Kuo, J. Lin, T.H. Tang, Human glucose-6-phosphate dehydrogenase (G6PD) gene transforms NIH 3T3 cells and induces tumors in nude mice. Int. J. Cancer, 85 (2000) 857.
  • D.S. Shereve, H.R. Levy, On the molecular weight of human glucose 6- phosphate dehydrogenase. Biochem. Biophy. Com, 78 (1977) 1369.
  • A.L. Lehninger, D.L. Nelson, M.M. Cox, Principles of Biochemistry, 2nd Ed. New York: Worth Publishers Inc, (1993) 436.
  • I. Ozmen, O.I. Kufrevioglu, M. Gul, Investigation of effects of some antibiotics on activities of glucose 6-phosphate dehydrogenase and antioxidant enzymes in erythrocytes. Drug Chem. Toxicol, 28 (2005) 433.
  • M.I. Kanji, M.L. Toews, W.R. Carpar, A kinetic study of glucose-6-phosphate dehydrogenase. J. Biol. Chem, 251 (1976) 2258.
  • D. Bradley, FDA approval provides new option against breast cancer. Pharm. Sci. Technology, 2 (1999) 471.
  • C. Jacquin, X. Nasica, D. Guillard, O. Aujoulat, C. Stoeckel, Intravitreal treatment of endophthalmitis traitement intravitreen des endophtalmies. Médecine et Maladies Infectieuses, 31 (2001) 493.
  • A. Mehta, P.J. Mason, T.J. Vulliamy, Glucose-6- phosphate dehydrogenase deficiency. Best Pract. Res. Clin. Haematol, 13 (2000) 21.
  • N. Basaran, Medical Genetic. Istanbul: Gunes & Nobel Medical Press, (1983) 327.
  • M. Gul, F.Z. Kutay, S. Temocin, O. Hanninen, Cellular and clinical implications of glutathione. Indian J. Exp. Biol, 28 (2000) 625.
  • B. Heinzt, T.A. Bock, H. Kierdorf, N. Maurin, Haemolytic crisis after acetaminophen in glucose-6-phosphate dehydrogenase deficiency. Klin. Wochenschr, 67 (1989) 1068.
  • T. Meloni, G. Forteleoni, F. Ena, G.F. Meloni, Glucose- 6-phosphate dehydrogenase deficiency and bacterial infections in northern Sardinia. J. Pediatr, 118 (1991) 909.
  • A. Szeinberg, C. Sheba, N. Hirsborn, E. Bodonyi, Studies on erthrocytes in cases with past history of favism and drug-induced acute hemolytic anemia. Blood, 12 (1957) 603.
  • T. Honjo, A. Watanabe, Clinical experience on sulbactam/cefoperazone in the pediatric field. Jpn. J.
  • Antibiot, 37 (1984) 32.
  • P.J. Dalgarno, D. Shewan, Illicit use of ketamine in Scotland. J. Psychoactive Drugs. 28 (1996) 191.
  • H.N. Wenk, M.N. Nannenga, C.N. Honda, Effect of morphine sulphate eye drops on hyperalgesia in the rat cornea. Pain, 105 (2003) 455.
  • S.O. Kayaalp, Rasyonel Tedavi Yönünden Tıbbi Farmakoloji. Ankara, Hacettepe-Tas Yayincilik, 2002.
  • P. Ninfali, T. Orsenigo, L. Barociani, S. Rapa, Rapid purification of glucose-6-phosphate dehydrogenase from mammal’s erythrocytes. Prep. Biochem., 20 (1990) 297.
  • A. Morelli, U. Benatti, G.F. Gaetani, A. De Flora, Biochemical mechanisms of glucose-6-phosphate dehydrogenase deficiency.Proc. Natl. Acad. Sci, 75 (1978) 1979.
  • M.M. Bradford, A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem, 72 (1976) 248.
  • D.K. Laemmli, Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227 (1970) 680.
  • E. Beutler, G6PD deficiency. Blood, 81 (1994) 3613.
  • D.R. Laurence, P.N. Bennett, M.J. Brown: Clinical Pharmacology. 8th edn., Churchill Livingstone, Singapore, 1997.
  • E.E. Keha, O.I. Kufrevioglu, Biyokimya, 5th ed., Istanbul, Aktif Yayinevi, 2007.
  • G.F. Gaetani, S. Galiano, L. Canepa, A.M. Ferraris, H.N. Kirkman, Catalase and glutathione peroxidase are equally active in detoxification of hydrogen peroxide in human erythrocytes. Blood, 73 (1989) 334.
  • J. Deutsch, Glucose-6-phosphate dehydrogenase. In: H.U. Bergmeyer, J. Bergmeyer Eds. Methods of enzymatic analysis, Verlags gerellschaff, VCH. 3 (1983) 190.
  • I. Ozmen, Effects of some chemicals on G6PD enzyme purified by affinity chromatography. Hacettepe J. Biol. Chem., 37 (2009) 327.
  • S. Altikat, M. Ciftci, M.E. Buyukokuroglu, In vitro effects of some anesthetic drugs on enzymatic activity of human red blood cell glucose 6-phosphate dehydrogenase. Polish J. Pharmacol., 54 (2002) 67.
  • A. Sahin, M. Senturk, M. Ciftci, E. Varoglu, O.I. 30. M. Senturk, S.B. Ceyhun, O. Erdogan, O.I. Kufrevioglu, Kufrevioglu, Effects of thallium-201 and radioactivity on human erythrocyte glucose 6-phosphate dehydrogenase activity. Nucl. Med. Biol., 37 (2010) 389.
  • In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (oncorhynchus mykiss) erythrocytes. Pestic. Biochem. Phys., 95 (2009) 95.

Bazı Organik Bileşikler ve Amino Asitlerin Glukoz 6-Fosfat Dehidrogenaz Aktivatör Özellikleri

Year 2011, Volume: 39 Issue: 2, 157 - 162, 01.04.2011

Zafer Ömer Özdemir Murat Şentürk Deniz Ekinci

Abstract

Bu çalışmanın amacı 4- metilsülfonil -asetofenon, 4- metilsülfonil fenilasetik asit, tirozin ve fenilalaninin insan eritrosit glukoz 6-fosfat dehidrogenaz üzerindeki aktivatör potansiyelini değerlendirmektir. Bu amaçla, öncelikle eritrosit glukoz 6-fosfat dehidrogenaz 2’,5’-ADP Sepharose 4B afinite jeli kullanılarak 812-kat, %40.6 verimle elde edildi. 4- Metilsülfonil fenilasetik asit, tirozin ve fenilalaninin enzim üzerinde aktivatör etkileri gösterdi. Bu bileşikler için % aktivite-[Aktivatör] grafiği kullanılarak % aktivite değerleri belirlendi.

Keywords

Aktivatör amino asit enzim glukoz 6-fosfat dehidrogenaz

References

  • W. Kuo, J. Lin, T.H. Tang, Human glucose-6-phosphate dehydrogenase (G6PD) gene transforms NIH 3T3 cells and induces tumors in nude mice. Int. J. Cancer, 85 (2000) 857.
  • D.S. Shereve, H.R. Levy, On the molecular weight of human glucose 6- phosphate dehydrogenase. Biochem. Biophy. Com, 78 (1977) 1369.
  • A.L. Lehninger, D.L. Nelson, M.M. Cox, Principles of Biochemistry, 2nd Ed. New York: Worth Publishers Inc, (1993) 436.
  • I. Ozmen, O.I. Kufrevioglu, M. Gul, Investigation of effects of some antibiotics on activities of glucose 6-phosphate dehydrogenase and antioxidant enzymes in erythrocytes. Drug Chem. Toxicol, 28 (2005) 433.
  • M.I. Kanji, M.L. Toews, W.R. Carpar, A kinetic study of glucose-6-phosphate dehydrogenase. J. Biol. Chem, 251 (1976) 2258.
  • D. Bradley, FDA approval provides new option against breast cancer. Pharm. Sci. Technology, 2 (1999) 471.
  • C. Jacquin, X. Nasica, D. Guillard, O. Aujoulat, C. Stoeckel, Intravitreal treatment of endophthalmitis traitement intravitreen des endophtalmies. Médecine et Maladies Infectieuses, 31 (2001) 493.
  • A. Mehta, P.J. Mason, T.J. Vulliamy, Glucose-6- phosphate dehydrogenase deficiency. Best Pract. Res. Clin. Haematol, 13 (2000) 21.
  • N. Basaran, Medical Genetic. Istanbul: Gunes & Nobel Medical Press, (1983) 327.
  • M. Gul, F.Z. Kutay, S. Temocin, O. Hanninen, Cellular and clinical implications of glutathione. Indian J. Exp. Biol, 28 (2000) 625.
  • B. Heinzt, T.A. Bock, H. Kierdorf, N. Maurin, Haemolytic crisis after acetaminophen in glucose-6-phosphate dehydrogenase deficiency. Klin. Wochenschr, 67 (1989) 1068.
  • T. Meloni, G. Forteleoni, F. Ena, G.F. Meloni, Glucose- 6-phosphate dehydrogenase deficiency and bacterial infections in northern Sardinia. J. Pediatr, 118 (1991) 909.
  • A. Szeinberg, C. Sheba, N. Hirsborn, E. Bodonyi, Studies on erthrocytes in cases with past history of favism and drug-induced acute hemolytic anemia. Blood, 12 (1957) 603.
  • T. Honjo, A. Watanabe, Clinical experience on sulbactam/cefoperazone in the pediatric field. Jpn. J.
  • Antibiot, 37 (1984) 32.
  • P.J. Dalgarno, D. Shewan, Illicit use of ketamine in Scotland. J. Psychoactive Drugs. 28 (1996) 191.
  • H.N. Wenk, M.N. Nannenga, C.N. Honda, Effect of morphine sulphate eye drops on hyperalgesia in the rat cornea. Pain, 105 (2003) 455.
  • S.O. Kayaalp, Rasyonel Tedavi Yönünden Tıbbi Farmakoloji. Ankara, Hacettepe-Tas Yayincilik, 2002.
  • P. Ninfali, T. Orsenigo, L. Barociani, S. Rapa, Rapid purification of glucose-6-phosphate dehydrogenase from mammal’s erythrocytes. Prep. Biochem., 20 (1990) 297.
  • A. Morelli, U. Benatti, G.F. Gaetani, A. De Flora, Biochemical mechanisms of glucose-6-phosphate dehydrogenase deficiency.Proc. Natl. Acad. Sci, 75 (1978) 1979.
  • M.M. Bradford, A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem, 72 (1976) 248.
  • D.K. Laemmli, Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227 (1970) 680.
  • E. Beutler, G6PD deficiency. Blood, 81 (1994) 3613.
  • D.R. Laurence, P.N. Bennett, M.J. Brown: Clinical Pharmacology. 8th edn., Churchill Livingstone, Singapore, 1997.
  • E.E. Keha, O.I. Kufrevioglu, Biyokimya, 5th ed., Istanbul, Aktif Yayinevi, 2007.
  • G.F. Gaetani, S. Galiano, L. Canepa, A.M. Ferraris, H.N. Kirkman, Catalase and glutathione peroxidase are equally active in detoxification of hydrogen peroxide in human erythrocytes. Blood, 73 (1989) 334.
  • J. Deutsch, Glucose-6-phosphate dehydrogenase. In: H.U. Bergmeyer, J. Bergmeyer Eds. Methods of enzymatic analysis, Verlags gerellschaff, VCH. 3 (1983) 190.
  • I. Ozmen, Effects of some chemicals on G6PD enzyme purified by affinity chromatography. Hacettepe J. Biol. Chem., 37 (2009) 327.
  • S. Altikat, M. Ciftci, M.E. Buyukokuroglu, In vitro effects of some anesthetic drugs on enzymatic activity of human red blood cell glucose 6-phosphate dehydrogenase. Polish J. Pharmacol., 54 (2002) 67.
  • A. Sahin, M. Senturk, M. Ciftci, E. Varoglu, O.I. 30. M. Senturk, S.B. Ceyhun, O. Erdogan, O.I. Kufrevioglu, Kufrevioglu, Effects of thallium-201 and radioactivity on human erythrocyte glucose 6-phosphate dehydrogenase activity. Nucl. Med. Biol., 37 (2010) 389.
  • In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (oncorhynchus mykiss) erythrocytes. Pestic. Biochem. Phys., 95 (2009) 95.
There are 31 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Zafer Ömer Özdemir

Murat Şentürk This is me

Deniz Ekinci This is me

Publication Date April 1, 2011
Published in Issue Year 2011 Volume: 39 Issue: 2

Cite

APA Özdemir, Z. Ö., Şentürk, M., & Ekinci, D. (2011). Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids. Hacettepe Journal of Biology and Chemistry, 39(2), 157-162.
AMA Özdemir ZÖ, Şentürk M, Ekinci D. Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids. HJBC. April 2011;39(2):157-162.
Chicago Özdemir, Zafer Ömer, Murat Şentürk, and Deniz Ekinci. “Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids”. Hacettepe Journal of Biology and Chemistry 39, no. 2 (April 2011): 157-62.
EndNote Özdemir ZÖ, Şentürk M, Ekinci D (April 1, 2011) Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids. Hacettepe Journal of Biology and Chemistry 39 2 157–162.
IEEE Z. Ö. Özdemir, M. Şentürk, and D. Ekinci, “Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids”, HJBC, vol. 39, no. 2, pp. 157–162, 2011.
ISNAD Özdemir, Zafer Ömer et al. “Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids”. Hacettepe Journal of Biology and Chemistry 39/2 (April 2011), 157-162.
JAMA Özdemir ZÖ, Şentürk M, Ekinci D. Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids. HJBC. 2011;39:157–162.
MLA Özdemir, Zafer Ömer et al. “Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids”. Hacettepe Journal of Biology and Chemistry, vol. 39, no. 2, 2011, pp. 157-62.
Vancouver Özdemir ZÖ, Şentürk M, Ekinci D. Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids. HJBC. 2011;39(2):157-62.

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