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Year 2023, Volume: 40 Issue: 3, 86 - 97, 27.11.2023

Hakan Aktaş Gizem Gökçe Akkaya Ebru Akyüz Çağdaş Gülden Haspolat Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.16882/hortis.1363441

Abstract

References

  • Akbari, H., Pajooheshgar, R., & Karimi, N. (2014). Evaluating the micropropagation of lisianthus (Eustoma grandiflora L.) as an important ornamental plant. Indian Journal of Fundamental and Applied Life Sciences, 4(2): 596-602.
  • Barna, K.S., & Wakhlu, A.K. (1995). Modified single node culture method – a new micropropagation method for chickpea. In Vitro Cellular & Developmental Biology – Plant, 31(3): 150–152.
  • Bejaoui, R. (2022). In vitro micropropriation of Kalanchoe (Kalanchoe blossfeldiana Poelln.). PhD Thesis, Ankara University, Ankara, 113 p (in Turkish).
  • Bertoldo, C., Gilardi, G., Spadaro, D., Gullino, M.L., & Garibaldi, A. (2015). Genetic diversity and virulence of Italian strains of Fusarium oxysporum isolated from Eustoma grandiflorum. European Journal of Plant Pathology, 141(1): 83-97.
  • Bhatia, S., & Sharma, K. (2015). Micropropagation. Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, 361–368.
  • Clapa, D., Fira, A., Dumitras, A., & Ciorchina, N. (2011). Înrădăcinarea şi aclimatizarea ex vitro în hidrocultură prin flotaţie a unor genotipuri de mur. Revista Botanică, 3(1): 133-139 (in Romanian).
  • Deroles, S.C., Ledger, S.E., Miller, R.M., Davies, K.M., & Given, N.K. (1993). Transformation in Eustoma grandiflorum (lisianthus). In: Plant Protoplasts and Genetic Engineering III. (pp. 202-212).
  • Duong, T.N., Nguyen, S.T., Ngoc, H.M., Uyen, P.N., Don, N.T., Mai, N.T., & Teixeira da Silva, J.A. (2006). Somatic embryogenesis induction from in vitro leaf cultures of Lisianthus (Eustoma grandiflorum (Raf.) Shinn.). Propagation of Ornamental Plants, 6(3): 121–127.
  • Esizad, S.G., Kaviani, B., Tarang, A., & Zanjani, S.B. (2012). Micropropagation of lisianthus (Eustoma grandiflorum), an ornamental plant. Plant Omics, 5(3): 314-319.
  • Furukawa, H. (1993). Some characteristics of regenerated plants from leaf and root explants of Eustoma grandiflorum. Plant Tissue Culture Letters, 10(1): 98-99.
  • Ghanati, F., Rezaee, F., & Boroujeni, L.Y. (2012). Micropropagation of Lisianthus (Eustoma grandiflora L.) from different explants to flowering onset. Iranian Journal of Plant Physiology, 3(1): 583-587.
  • Handa, T., & Deroles, S.C. (2001). Transgenic Eustoma grandiflorum (Lisianthus). In: Transgenic Crops III. Biotechnology in Agriculture and Forestry, 107-122.
  • Hanks, G. (2014). Lisianthus (Eustoma grandiflorum) as a cut flower crop grown in polythene tunnels. Horticultural Development Company. Warwickshire. 6 pp.
  • Harbaugh, B.K., & Zhanao, D. (2006). UF Double Joy cultivar group—five colors of double-flowering and heat-tolerant lisianthus for potted plants. HortScience, 41(3): 846-849.
  • Harbaugh, B.K. (2007). Lisianthus Eustoma grandiflorum. Editor: Anderson NO. Flower Breeding and Genetics, 1st ed., Springer, Dordrecht, 645-663.
  • Haspolat, G., Bejaoui, R., Vural, G.E., & Ellialtioglu, S.S. (2020). Lisianthus (Eustoma grandiflorum) Islahı ve Gelecek Projeksiyonu, Ziraat Orman ve Su Ürünleri Alanında Teoriler II, Gece Kitaplığı, 73-112 (in Turkish).
  • Hutchinson, M.J., Muchiri, J.N., & Waithaka, K. (2011). Cold storage and flower keeping quality of cut lisianthus (Eustoma grandiflorum L.). Botswana Journal of Agriculture and Applied Sciences, 7: 4-11.
  • Ibrahim, A.O.S. (2022). Researches on using of float hydroculture for ex vitro rooting of microcuttings and acclimatization of in vitro plantlets in some Pyrus species. Ankara University, Graduate School of Natural and Applied Sciences, Department of Horticulture, PhD Thesis, Ankara, 106 p. (in Turkish).
  • Jafari, R., Moieni, A., Karimzadeh, G., & Movahedi, Z. (2017). In vitro propagation of lisianthus (Eustoma grandiflurom). Journal of Plant Physiology and Breeding, 7(2): 53-64.
  • Kabakci, M. (1996). Lisianthus (Eustoma grandiflorum c.v. Royal F1 Purple)’un Mikroçoğaltımı Üzerine Araştırmalar. MSc Thesis, Ege University, İzmir, (in Turkish).
  • Kaviani, B. (2014). Micropropagation of ten weeks (Matthiola incana) and lisianthus (Eustoma grandiflorum) (Two Ornamental Plants) by using Kinetin (KIN), Naphthalene Acetic Acid (NAA) and 2,4-Dichlorophenoxy Acetic Acid (2,4-D). Acta Scientarum Polonorum Hortorum Cultus, 13(1): 141-154.
  • Kaviani, B., Zamiraee, F., Zanjani, S.B., Tarang, A., & Torkashvand, A.M. (2014). In vitro flowering and micropropagation of lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA). Acta Sci. Polonorum Hortorum Cultus, 13(4): 145-155.
  • Lineberger, R.D. (1983). Shoot proliferation, rooting, and transplant survival of tissue-cultured" Hally Jolivette" cherry. Hortscience, 18(2): 182-185.
  • Martini, A.N., Vlachou, G., & Papafotiou, M. (2022). Effect of explant origin and medium plant growth regulators on in vitro shoot proliferation and rooting of Salvia tomentosa, a native sage of the Northeastern Mediterranean Basin. Agronomy, 12(8): 1889.
  • Menge, G.A. (2019). Hasat Sonrası Sakkaroz ve Etanol Uygulamalarının Bazı Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Çeşitlerinin Fizyolojik Özellikleri Üzerine Etkileri. MSc Thesis, Eskişehir Osmangazi University, Eskişehir, (in Turkish).
  • Miri, S.M., Savari, A., Behzad, K., & Iravani, B.M. (2016). Promotion of callus initiation, shoot regeneration and proliferation in lisianthus. Iranian Journal of Plant Physiology, 6(4): 1855-1860.
  • Mizushima, S. (2017). Cutting propagation using the single-node culture method in Elatostema involucratum Franch. & Sav. Journal of Science and High Technology in Agriculture, 29(2): 56-59.
  • Mousavi, E.S., Behbahani, M., Hadavi, E., Miri, S.M., & Karimi, N. (2012). Plant regeneration in Eustoma grandiflorum from axillaries buds (Gentianaceae). Trakia Journal of Sciences, 10(2): 75-78.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
  • Nemeth, G. (1986). Induction of Rooting. In Biotechnology in Agriculture and Forestry, Trees I. (pp. 49-64)
  • Nhut, D. T., Tuan, N.S., Ngoc, H.M., Uyen, P.N., Don, N.T., Mai, N.T., & De Silva, J.T. (2006). Somatic embryogenesis induction from in vitro leaf cultures of lisianthus (Eustoma grandiflorum (Raf.) Shinn.). Propagation of Ornamental Plants, 6(3): 121-127.
  • Okay, Y., Ellialtioğlu, Ş., Demir, K., Tipirdamaz, R., Savci, A.E., Özler, H., Gümüş, C., & Günöz, A. (2014). Sentorya (Centaurea tchihatchefii Fish. &Mey.) endemik bitkisinin çoğaltımı üzerinde çalışmalar. Biyoloji Bilimleri Araştırma Dergisi, 7(1): 42-48 (in Turkish).
  • Ordogh, M.‚ Jambor-Benczur, E., & Tilly-Mandy, A. (2006) Micropropagation of Echo cultivars of Eustoma grandiflorum. Acta Horticulture, 725: 457–460.
  • Ozçelik, A. (2000). Lisianthus (Eustoma grandiflorum) Yetiştiriciliğinde Rozetleşme, Bitki Gelişimi ve Verime Vernalizasyon ile GA3'ün Etkileri. PhD Thesis, Ege University, İzmir, (in Turkish).
  • Ozkan, H. (2017). Lisianthus [Eustoma grandiflorum (Raf.) Shinn. cv. 'Mariachi Pure White (F1)'] Süs Bitkisinin Organogenez ile Mikroçoğaltımı. MsC Thesis, Kocaeli University, Kocaeli, (in Turkish).
  • Paek, K.Y., & Hahn, E.J. (2000). Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus [Eustoma grandiflorum (Raf.) Shinn.]. In Vitro Cellular & Developmental Biology-Plant, 36: 128-132.
  • Pop, R., Cantor, M., Buta, E., & Csete, I. (2016). In vitro plant propagation and crop ımprovement in lisianthus (Lisianthus russelianus Hook.). Bulletin UASVM Hort., 73(2): 168-174.
  • Price, J.F., Harbaugh, B.K., & McGovern, R.J. (1998). Potted lisianthus: Secrets of success for controlling insects. Greenhouse Grower, 16(2): 22-27.
  • Rahaman, S.K.S.R., Adhikari, P., Islam, S., Banerjee, A., & Uddin, A.F.M.J. (2018). Effect of NAA for callus induction of lisianthus [Eustoma grandiflorum (Raf.) Shinn] on MS and B5 medium. Advances in Floriculture and Urban Horticulture Proceedings of the National Symposium on Recent Advances in Floriculture and Urban Horticulture in Global Perspective, 4-5th January, West Bengal/ India.
  • Rezaee, F., Ghanati, F., & Yusefzadeh, B.L. (2012). Micropropagation of Lisianthus (Eustoma grandiflorum L.) from different explants to flowering onset. Iranian Journal of Plant Physiology, 3(1): 583-587.
  • Saeed, N.A., Zafar, Y., & Malik, K.A. (1997). A simple procedure of Gossypium meristem shoots tip culture. Plant Cell Tissue and Organ Culture, 51(3): 201–207.
  • Semeniuk, P., & Griesbach, R.J. (1987). In vitro propagation of prairie gentian. Plant Cell, Tissue and Organ Culture, 8: 249-253.
  • Semeria, L., Ruffoni, B., Rabaglio, M., Genga, A., Vaira, A.M., Accotto, G.P., & Allavena, A. (1996). Genetic transformation ofEustoma grandiflorum by Agrobacterium tumefaciens. Plant Cell Tiss Organ Culture, 47: 67–72.
  • Skirvin, R.M., Chu, M.C., & Rukan, H. (1980). Rooting studies with Prunus-supp in vitro. HortScience, 15(3): 415-415.
  • Tepe, Ş., Ellialtioğlu, Ş., Yenice, N., & Tipirdamaz, R. (2002). In vitro kolhisin uygulaması ile poliploid nane (Mentha longifolia L.) bitkilerinin elde edilmesi. Akdeniz University Journal of the Faculty of Agriculture, 15(2): 63-69 (in Turkish).
  • Türközü, D., Yaşar, F., Ellialtioğlu, Ş., & Yildirim, B. (2014). Tarhun (Artemisia dracunculus L.) bitkisinin doku kültürü yoluyla çoğaltılması üzerinde çalışmalar. Yuzuncu Yıl University Journal of Agricultural Sciences, 24(3): 300-308 (in Turkish).
  • Uddin, A.F.M.J., Rahman, S.S., Ahmad, H., Parvin, S., & Momena, K. (2017). In vitro regeneration of lisianthus (Eustoma grandiflorum Grise). International Journal of Business, Social and Scientific Research, 5(2): 126-135.
  • Yumbla-Orbes, M., Rocha, D.I., de Matos, E.M., Koehler, A.D., Pinheiro, M.V.M., Batista, D.S., & Otoni, W.C. (2020). Somatic embryogenesis induced from vascular tissues in leaf explants of lisianthus (Eustoma grandiflorum (Raf.) Shinn) generates true-to-type diploid plants. Vegetos, 33(1): 135-144.

Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators

Year 2023, Volume: 40 Issue: 3, 86 - 97, 27.11.2023

Hakan Aktaş Gizem Gökçe Akkaya Ebru Akyüz Çağdaş Gülden Haspolat Şeküre Şebnem Ellialtıoğlu

https://doi.org/10.16882/hortis.1363441

Abstract

Lisianthus has long-lasting, white, blue-purple, velvet or pink flowers. Varieties that can be used in pots, outdoors, or cut flowers are widely used and it is essential to develop new varieties to increase their commercial value. Breeding studies with classical methods can take a long time in lisianthus. Techniques to create variation in these plants and facilitate their reproduction always contribute to shortening the breeding period and one of the best known of these methods is plant tissue culture. This study aimed to investigate the micropropagation possibilities in lisianthus and examine the effects of auxin and cytokinin applications and doses on shoot reproduction. Supplemented with 10 different plant growth regulators in Murashige and Skoog (MS) medium, leaf explants from lisianthus plants that were germinated under in vitro conditions (seedlings) were cultivated. 6-benzyl aminopurine (BAP) was utilized in 5 doses (0.5, 1.0, 2.0, 3.0, and 4.0 mg L-1) alone in the first 5 groups. Naphthalene acetic acid (NAA) was also added to the BAP mediums at a concentration of 0.5 mg L-1. Shoot formation, shoot lengths, the number of shoots longer and shorter than 1 cm, and regeneration of new shoots after subcultures were determined. According to the results, MS mediums containing 3.0 and 4.0 mg L-1 BAP could be good starting mediums for regenerating new lisianthus shoots from leaf explants. These treatments got the highest values both in shoot formation and the number of shoots longer than 1 cm. It has been determined that 3.0 mg L-1 BAP alone can be used for both organogenesis from leaf explants and shoot proliferation in single node culture. In vitro shoots of the lisianthus species were easily rooted and both PGR-free MS or ½ MS mediums. Lisianthus shoots propagated under in vitro conditions can be rooted under ex vitro conditions both in the float hydroculture and in the soil mixture. The acclimatization stage was optimized also successfully.

Keywords

6-benzyl aminopurine Ex vitro rooting In vitro propagation Lisianthus Naphthalene acetic acid

References

  • Akbari, H., Pajooheshgar, R., & Karimi, N. (2014). Evaluating the micropropagation of lisianthus (Eustoma grandiflora L.) as an important ornamental plant. Indian Journal of Fundamental and Applied Life Sciences, 4(2): 596-602.
  • Barna, K.S., & Wakhlu, A.K. (1995). Modified single node culture method – a new micropropagation method for chickpea. In Vitro Cellular & Developmental Biology – Plant, 31(3): 150–152.
  • Bejaoui, R. (2022). In vitro micropropriation of Kalanchoe (Kalanchoe blossfeldiana Poelln.). PhD Thesis, Ankara University, Ankara, 113 p (in Turkish).
  • Bertoldo, C., Gilardi, G., Spadaro, D., Gullino, M.L., & Garibaldi, A. (2015). Genetic diversity and virulence of Italian strains of Fusarium oxysporum isolated from Eustoma grandiflorum. European Journal of Plant Pathology, 141(1): 83-97.
  • Bhatia, S., & Sharma, K. (2015). Micropropagation. Modern Applications of Plant Biotechnology in Pharmaceutical Sciences, 361–368.
  • Clapa, D., Fira, A., Dumitras, A., & Ciorchina, N. (2011). Înrădăcinarea şi aclimatizarea ex vitro în hidrocultură prin flotaţie a unor genotipuri de mur. Revista Botanică, 3(1): 133-139 (in Romanian).
  • Deroles, S.C., Ledger, S.E., Miller, R.M., Davies, K.M., & Given, N.K. (1993). Transformation in Eustoma grandiflorum (lisianthus). In: Plant Protoplasts and Genetic Engineering III. (pp. 202-212).
  • Duong, T.N., Nguyen, S.T., Ngoc, H.M., Uyen, P.N., Don, N.T., Mai, N.T., & Teixeira da Silva, J.A. (2006). Somatic embryogenesis induction from in vitro leaf cultures of Lisianthus (Eustoma grandiflorum (Raf.) Shinn.). Propagation of Ornamental Plants, 6(3): 121–127.
  • Esizad, S.G., Kaviani, B., Tarang, A., & Zanjani, S.B. (2012). Micropropagation of lisianthus (Eustoma grandiflorum), an ornamental plant. Plant Omics, 5(3): 314-319.
  • Furukawa, H. (1993). Some characteristics of regenerated plants from leaf and root explants of Eustoma grandiflorum. Plant Tissue Culture Letters, 10(1): 98-99.
  • Ghanati, F., Rezaee, F., & Boroujeni, L.Y. (2012). Micropropagation of Lisianthus (Eustoma grandiflora L.) from different explants to flowering onset. Iranian Journal of Plant Physiology, 3(1): 583-587.
  • Handa, T., & Deroles, S.C. (2001). Transgenic Eustoma grandiflorum (Lisianthus). In: Transgenic Crops III. Biotechnology in Agriculture and Forestry, 107-122.
  • Hanks, G. (2014). Lisianthus (Eustoma grandiflorum) as a cut flower crop grown in polythene tunnels. Horticultural Development Company. Warwickshire. 6 pp.
  • Harbaugh, B.K., & Zhanao, D. (2006). UF Double Joy cultivar group—five colors of double-flowering and heat-tolerant lisianthus for potted plants. HortScience, 41(3): 846-849.
  • Harbaugh, B.K. (2007). Lisianthus Eustoma grandiflorum. Editor: Anderson NO. Flower Breeding and Genetics, 1st ed., Springer, Dordrecht, 645-663.
  • Haspolat, G., Bejaoui, R., Vural, G.E., & Ellialtioglu, S.S. (2020). Lisianthus (Eustoma grandiflorum) Islahı ve Gelecek Projeksiyonu, Ziraat Orman ve Su Ürünleri Alanında Teoriler II, Gece Kitaplığı, 73-112 (in Turkish).
  • Hutchinson, M.J., Muchiri, J.N., & Waithaka, K. (2011). Cold storage and flower keeping quality of cut lisianthus (Eustoma grandiflorum L.). Botswana Journal of Agriculture and Applied Sciences, 7: 4-11.
  • Ibrahim, A.O.S. (2022). Researches on using of float hydroculture for ex vitro rooting of microcuttings and acclimatization of in vitro plantlets in some Pyrus species. Ankara University, Graduate School of Natural and Applied Sciences, Department of Horticulture, PhD Thesis, Ankara, 106 p. (in Turkish).
  • Jafari, R., Moieni, A., Karimzadeh, G., & Movahedi, Z. (2017). In vitro propagation of lisianthus (Eustoma grandiflurom). Journal of Plant Physiology and Breeding, 7(2): 53-64.
  • Kabakci, M. (1996). Lisianthus (Eustoma grandiflorum c.v. Royal F1 Purple)’un Mikroçoğaltımı Üzerine Araştırmalar. MSc Thesis, Ege University, İzmir, (in Turkish).
  • Kaviani, B. (2014). Micropropagation of ten weeks (Matthiola incana) and lisianthus (Eustoma grandiflorum) (Two Ornamental Plants) by using Kinetin (KIN), Naphthalene Acetic Acid (NAA) and 2,4-Dichlorophenoxy Acetic Acid (2,4-D). Acta Scientarum Polonorum Hortorum Cultus, 13(1): 141-154.
  • Kaviani, B., Zamiraee, F., Zanjani, S.B., Tarang, A., & Torkashvand, A.M. (2014). In vitro flowering and micropropagation of lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA). Acta Sci. Polonorum Hortorum Cultus, 13(4): 145-155.
  • Lineberger, R.D. (1983). Shoot proliferation, rooting, and transplant survival of tissue-cultured" Hally Jolivette" cherry. Hortscience, 18(2): 182-185.
  • Martini, A.N., Vlachou, G., & Papafotiou, M. (2022). Effect of explant origin and medium plant growth regulators on in vitro shoot proliferation and rooting of Salvia tomentosa, a native sage of the Northeastern Mediterranean Basin. Agronomy, 12(8): 1889.
  • Menge, G.A. (2019). Hasat Sonrası Sakkaroz ve Etanol Uygulamalarının Bazı Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Çeşitlerinin Fizyolojik Özellikleri Üzerine Etkileri. MSc Thesis, Eskişehir Osmangazi University, Eskişehir, (in Turkish).
  • Miri, S.M., Savari, A., Behzad, K., & Iravani, B.M. (2016). Promotion of callus initiation, shoot regeneration and proliferation in lisianthus. Iranian Journal of Plant Physiology, 6(4): 1855-1860.
  • Mizushima, S. (2017). Cutting propagation using the single-node culture method in Elatostema involucratum Franch. & Sav. Journal of Science and High Technology in Agriculture, 29(2): 56-59.
  • Mousavi, E.S., Behbahani, M., Hadavi, E., Miri, S.M., & Karimi, N. (2012). Plant regeneration in Eustoma grandiflorum from axillaries buds (Gentianaceae). Trakia Journal of Sciences, 10(2): 75-78.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
  • Nemeth, G. (1986). Induction of Rooting. In Biotechnology in Agriculture and Forestry, Trees I. (pp. 49-64)
  • Nhut, D. T., Tuan, N.S., Ngoc, H.M., Uyen, P.N., Don, N.T., Mai, N.T., & De Silva, J.T. (2006). Somatic embryogenesis induction from in vitro leaf cultures of lisianthus (Eustoma grandiflorum (Raf.) Shinn.). Propagation of Ornamental Plants, 6(3): 121-127.
  • Okay, Y., Ellialtioğlu, Ş., Demir, K., Tipirdamaz, R., Savci, A.E., Özler, H., Gümüş, C., & Günöz, A. (2014). Sentorya (Centaurea tchihatchefii Fish. &Mey.) endemik bitkisinin çoğaltımı üzerinde çalışmalar. Biyoloji Bilimleri Araştırma Dergisi, 7(1): 42-48 (in Turkish).
  • Ordogh, M.‚ Jambor-Benczur, E., & Tilly-Mandy, A. (2006) Micropropagation of Echo cultivars of Eustoma grandiflorum. Acta Horticulture, 725: 457–460.
  • Ozçelik, A. (2000). Lisianthus (Eustoma grandiflorum) Yetiştiriciliğinde Rozetleşme, Bitki Gelişimi ve Verime Vernalizasyon ile GA3'ün Etkileri. PhD Thesis, Ege University, İzmir, (in Turkish).
  • Ozkan, H. (2017). Lisianthus [Eustoma grandiflorum (Raf.) Shinn. cv. 'Mariachi Pure White (F1)'] Süs Bitkisinin Organogenez ile Mikroçoğaltımı. MsC Thesis, Kocaeli University, Kocaeli, (in Turkish).
  • Paek, K.Y., & Hahn, E.J. (2000). Cytokinins, auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus [Eustoma grandiflorum (Raf.) Shinn.]. In Vitro Cellular & Developmental Biology-Plant, 36: 128-132.
  • Pop, R., Cantor, M., Buta, E., & Csete, I. (2016). In vitro plant propagation and crop ımprovement in lisianthus (Lisianthus russelianus Hook.). Bulletin UASVM Hort., 73(2): 168-174.
  • Price, J.F., Harbaugh, B.K., & McGovern, R.J. (1998). Potted lisianthus: Secrets of success for controlling insects. Greenhouse Grower, 16(2): 22-27.
  • Rahaman, S.K.S.R., Adhikari, P., Islam, S., Banerjee, A., & Uddin, A.F.M.J. (2018). Effect of NAA for callus induction of lisianthus [Eustoma grandiflorum (Raf.) Shinn] on MS and B5 medium. Advances in Floriculture and Urban Horticulture Proceedings of the National Symposium on Recent Advances in Floriculture and Urban Horticulture in Global Perspective, 4-5th January, West Bengal/ India.
  • Rezaee, F., Ghanati, F., & Yusefzadeh, B.L. (2012). Micropropagation of Lisianthus (Eustoma grandiflorum L.) from different explants to flowering onset. Iranian Journal of Plant Physiology, 3(1): 583-587.
  • Saeed, N.A., Zafar, Y., & Malik, K.A. (1997). A simple procedure of Gossypium meristem shoots tip culture. Plant Cell Tissue and Organ Culture, 51(3): 201–207.
  • Semeniuk, P., & Griesbach, R.J. (1987). In vitro propagation of prairie gentian. Plant Cell, Tissue and Organ Culture, 8: 249-253.
  • Semeria, L., Ruffoni, B., Rabaglio, M., Genga, A., Vaira, A.M., Accotto, G.P., & Allavena, A. (1996). Genetic transformation ofEustoma grandiflorum by Agrobacterium tumefaciens. Plant Cell Tiss Organ Culture, 47: 67–72.
  • Skirvin, R.M., Chu, M.C., & Rukan, H. (1980). Rooting studies with Prunus-supp in vitro. HortScience, 15(3): 415-415.
  • Tepe, Ş., Ellialtioğlu, Ş., Yenice, N., & Tipirdamaz, R. (2002). In vitro kolhisin uygulaması ile poliploid nane (Mentha longifolia L.) bitkilerinin elde edilmesi. Akdeniz University Journal of the Faculty of Agriculture, 15(2): 63-69 (in Turkish).
  • Türközü, D., Yaşar, F., Ellialtioğlu, Ş., & Yildirim, B. (2014). Tarhun (Artemisia dracunculus L.) bitkisinin doku kültürü yoluyla çoğaltılması üzerinde çalışmalar. Yuzuncu Yıl University Journal of Agricultural Sciences, 24(3): 300-308 (in Turkish).
  • Uddin, A.F.M.J., Rahman, S.S., Ahmad, H., Parvin, S., & Momena, K. (2017). In vitro regeneration of lisianthus (Eustoma grandiflorum Grise). International Journal of Business, Social and Scientific Research, 5(2): 126-135.
  • Yumbla-Orbes, M., Rocha, D.I., de Matos, E.M., Koehler, A.D., Pinheiro, M.V.M., Batista, D.S., & Otoni, W.C. (2020). Somatic embryogenesis induced from vascular tissues in leaf explants of lisianthus (Eustoma grandiflorum (Raf.) Shinn) generates true-to-type diploid plants. Vegetos, 33(1): 135-144.
There are 48 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering (Other)
Journal Section Araştırma Makalesi
Authors

Hakan Aktaş

Gizem Gökçe Akkaya This is me

Ebru Akyüz Çağdaş This is me

Gülden Haspolat This is me

Şeküre Şebnem Ellialtıoğlu

Early Pub Date September 29, 2023
Publication Date November 27, 2023
Published in Issue Year 2023 Volume: 40 Issue: 3

Cite

APA Aktaş, H., Akkaya, G. G., Akyüz Çağdaş, E., Haspolat, G., et al. (2023). Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators. Horticultural Studies, 40(3), 86-97. https://doi.org/10.16882/hortis.1363441
AMA Aktaş H, Akkaya GG, Akyüz Çağdaş E, Haspolat G, Ellialtıoğlu ŞŞ. Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators. HortiS. November 2023;40(3):86-97. doi:10.16882/hortis.1363441
Chicago Aktaş, Hakan, Gizem Gökçe Akkaya, Ebru Akyüz Çağdaş, Gülden Haspolat, and Şeküre Şebnem Ellialtıoğlu. “Micropropagation of Lisianthus [Eustoma Grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators”. Horticultural Studies 40, no. 3 (November 2023): 86-97. https://doi.org/10.16882/hortis.1363441.
EndNote Aktaş H, Akkaya GG, Akyüz Çağdaş E, Haspolat G, Ellialtıoğlu ŞŞ (November 1, 2023) Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators. Horticultural Studies 40 3 86–97.
IEEE H. Aktaş, G. G. Akkaya, E. Akyüz Çağdaş, G. Haspolat, and Ş. Ş. Ellialtıoğlu, “Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators”, HortiS, vol. 40, no. 3, pp. 86–97, 2023, doi: 10.16882/hortis.1363441.
ISNAD Aktaş, Hakan et al. “Micropropagation of Lisianthus [Eustoma Grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators”. Horticultural Studies 40/3 (November 2023), 86-97. https://doi.org/10.16882/hortis.1363441.
JAMA Aktaş H, Akkaya GG, Akyüz Çağdaş E, Haspolat G, Ellialtıoğlu ŞŞ. Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators. HortiS. 2023;40:86–97.
MLA Aktaş, Hakan et al. “Micropropagation of Lisianthus [Eustoma Grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators”. Horticultural Studies, vol. 40, no. 3, 2023, pp. 86-97, doi:10.16882/hortis.1363441.
Vancouver Aktaş H, Akkaya GG, Akyüz Çağdaş E, Haspolat G, Ellialtıoğlu ŞŞ. Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators. HortiS. 2023;40(3):86-97.
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