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Koagülasyon testlerinde optik ve mekanik pıhtı yöntemlerinin karşılaştırılması

Year 2019, Volume: 16 Issue: 1, 13 - 16, 22.03.2019

Abstract


Amaç:Tıbbi laboratuvarların büyüklüğü ve kapasitesine
göre kullandığı ölçüm yöntemleri değişkenlik göstermektedir. Çalışmamızda
yarı-otomatize sistemin kullandığı mekanik pıhtı saptama yöntemi ile tam
otomatize koagülasyon sistemlerinde var olan foto-optik pıhtı saptama
yönteminin karşılaştırılması amaçlanmıştır.

Materyal ve metod: 56
plazma örneğinden CoA Data 4004 ve de
Sysmex
CS-2000i/CS-
2100i koagülasyon
sistemleri ile protrombin zamanı
(PTZ), aktive parsiyel tromboplastin
zamanı
(APTT) düzeyleri ve uluslararası
normalleştirilmiş oran (
INR) çalışılmıştır. PTZ, APTT ve INR değerleri SPSS programları ile regresyon
analizi yapılarak ve Bland - Altman grafikleri uygulanarak değerlendirilmiştir.
CLIA total izin verilebilir hata ve dış kalite değerlendirme sonuçlarına göre
tam otomatize sistem referans kabul edilerek yarı otomatize sistemin performansı
hesaplanmıştır.

Bulgular:Lineer regresyon sonucu
PTZ için r= 0.844, INR için r=0.842 ve APTT için r=0.737 olarak
bulunmuştur.  Her üç testin Bland-Altman grafiklerinde sistematik
hata gözlenmemiştir. İki yöntem arasında PTZ ve INR için fark bulunmazken
(p=0,314&p=0,196), APTT için fark saptanmıştır (p=0,003). Çalışmamızda
CoA Data 4004 cihazının
APTT için daha düşük değerler verdiği gözlenmiştir.

Sonuç:Koagülasyon testlerinin doğru ve güvenilir izlemi için
standardize yöntemler ile analiz gerçekleştirilmelidir. Çalışmamızda APTT testi
için iki yöntem arası fark CLIA izin verilir total hatadan daha düşük olduğu
için yarı otomatize cihazın performasının kabul edilebilir olduğunu ortaya
koyduk.

References

  • Referans 1:Memişoğulları R., “Tıbbi Cihaz ve Malzeme Alımında Şartname Hazırlama ve İhale Süreci,” Düzce Tıp Fakültesi Derg.,2008:2,45–50
  • Referans 2:Smythe M. A., Priziola J., Dobesh P. P., Wirth D., Cuker A., and Wittkowsky A. K., “Guidance for the practical management of the heparin anticoagulants in the treatment of venous thromboembolism,” J. Thromb. Thrombolysis,2016:41(1)165–186
  • Referans 3:Kaserer A., Casutt M., Sprengel K., Seifert B., Spahn D. R., andStein P., “Comparison of two different coagulation algorithms on the use of allogenic blood products and coagulation factors in severely injured trauma patients: A retrospective, multicentre, observational study,” Scand. J. Trauma. Resusc. Emerg. Med., 2018:26(1)1–9
  • Referans 4:A. Mina, E. J. Favaloro, and J. Koutts, “Relationship between short activated partial thromboplastin times, thrombin generation, procoagulant factors and procoagulant phospholipid activity,” Blood Coagul. Fibrinolysis, 2012:23(3):203–207
  • Referans 5: Rathod N., Nair S., Mammen J., andSingh S., “A comparison study of routine coagulation screening tests (PT and APTT) by three automated coagulation analyzers,” Int. J. Med. Sci. Public Heal., 2016(5)8:1563
  • Referans 6:Ricos C, Alvareq V, Cava F, Garcia-Lario JV, Hernandez A, Jimenez CV, et al. Desirable specifications for total error, imprecision, and bias, derived from intra- and inter-individual biological variation, http://www.westgard.com/biodatabase 2014-update.htm
  • Referans 7:Tekkesin N. and Kilinc C., “Optical and Mechanical Clot Detection Methodologies: A Comparison Study for Routine Coagulation Testing,” J. Clin. Lab. Anal., 2012:(26)3: 125–129
  • Referans 8: Bai B., Christie D. J., Gorman R. T., and Wu J. R., “Comparison of optical and mechanical clot detection for routine coagulation testing in a large volume clinical laboratory,” Blood Coagul. Fibrinolysis, 2008:19(6)569–576
  • Referans 9:Ergin M., Erdogan S., Akturk O., and Erel O., “The effects of transport by car on coagulation tests,” Clin. Chem. Lab. Med., 2017:55(12)1943–1947
  • Referans 10: Aggarwal S,. Nayak D. M, and Manohar C., “Discrepancy in Optical & Mechanical Method in Coagulation Tests in a Turbid Sample,” Indian J. Hematol. Blood Transfus., 2014:(30)402–404
  • Referans 11: Hur et al., “Comparison of international normalized ratio measurement between coaguchek XS plus and STA-R coagulation analyzers,” Biomed Res. Int, 2013 http://dx.doi.org/10.1155/2013/213109

Comparison of optical and mechanical clot detection methods in coagulation analyses

Year 2019, Volume: 16 Issue: 1, 13 - 16, 22.03.2019

Abstract

Background: Measurement techniques vary in terms of laboratory capacity and requirement. In our study, we compared mechanical clot detection system in semi-automated coagulation system to photo-optical clot detection system that used in automated coagulation systems.

Methods: We analyzed, prothrombin time (PT), activated partial thromboplastin time (APTT) and international normalization ratio (INR) tests with CoA Data 4004 and Sysmex CS-2000i/CS2100i coagulation systems in 56 plasma samples. PT, APTT and INR levels analyzed with regression analyze, and Bland-Altman graphics using SPSS package program. Approving automated coagulation system as reference, we calculated semi-automated performance using with CLIA total allowable error and external quality reports.

Results: Correlation analysis results were r= 0.844 for PT, r=0.842 for INR and r=0.737 for APTT. There was no systematic error in Bland-Altman graphics. While there was no difference PT and INR levels (p=0,314&p=0,196), APTT (p=0,003) showed statistical difference between two methods. CoA Data 4004 device gave lower APTT results.

Conclusions: Monitoring coagulation tests are more reliable and accurate when the standardized methods used. In our study, we revealed semi-automated device performance was acceptable because of our results were lessen than CLIA total allowable error limits.

References

  • Referans 1:Memişoğulları R., “Tıbbi Cihaz ve Malzeme Alımında Şartname Hazırlama ve İhale Süreci,” Düzce Tıp Fakültesi Derg.,2008:2,45–50
  • Referans 2:Smythe M. A., Priziola J., Dobesh P. P., Wirth D., Cuker A., and Wittkowsky A. K., “Guidance for the practical management of the heparin anticoagulants in the treatment of venous thromboembolism,” J. Thromb. Thrombolysis,2016:41(1)165–186
  • Referans 3:Kaserer A., Casutt M., Sprengel K., Seifert B., Spahn D. R., andStein P., “Comparison of two different coagulation algorithms on the use of allogenic blood products and coagulation factors in severely injured trauma patients: A retrospective, multicentre, observational study,” Scand. J. Trauma. Resusc. Emerg. Med., 2018:26(1)1–9
  • Referans 4:A. Mina, E. J. Favaloro, and J. Koutts, “Relationship between short activated partial thromboplastin times, thrombin generation, procoagulant factors and procoagulant phospholipid activity,” Blood Coagul. Fibrinolysis, 2012:23(3):203–207
  • Referans 5: Rathod N., Nair S., Mammen J., andSingh S., “A comparison study of routine coagulation screening tests (PT and APTT) by three automated coagulation analyzers,” Int. J. Med. Sci. Public Heal., 2016(5)8:1563
  • Referans 6:Ricos C, Alvareq V, Cava F, Garcia-Lario JV, Hernandez A, Jimenez CV, et al. Desirable specifications for total error, imprecision, and bias, derived from intra- and inter-individual biological variation, http://www.westgard.com/biodatabase 2014-update.htm
  • Referans 7:Tekkesin N. and Kilinc C., “Optical and Mechanical Clot Detection Methodologies: A Comparison Study for Routine Coagulation Testing,” J. Clin. Lab. Anal., 2012:(26)3: 125–129
  • Referans 8: Bai B., Christie D. J., Gorman R. T., and Wu J. R., “Comparison of optical and mechanical clot detection for routine coagulation testing in a large volume clinical laboratory,” Blood Coagul. Fibrinolysis, 2008:19(6)569–576
  • Referans 9:Ergin M., Erdogan S., Akturk O., and Erel O., “The effects of transport by car on coagulation tests,” Clin. Chem. Lab. Med., 2017:55(12)1943–1947
  • Referans 10: Aggarwal S,. Nayak D. M, and Manohar C., “Discrepancy in Optical & Mechanical Method in Coagulation Tests in a Turbid Sample,” Indian J. Hematol. Blood Transfus., 2014:(30)402–404
  • Referans 11: Hur et al., “Comparison of international normalized ratio measurement between coaguchek XS plus and STA-R coagulation analyzers,” Biomed Res. Int, 2013 http://dx.doi.org/10.1155/2013/213109
There are 11 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Esin Avcı 0000-0002-9173-0142

Mügan Ercan

Onur Bahçeci This is me 0000-0003-2371-5044

Şahika Özen This is me

Publication Date March 22, 2019
Submission Date November 12, 2018
Acceptance Date January 31, 2019
Published in Issue Year 2019 Volume: 16 Issue: 1

Cite

Vancouver Avcı E, Ercan M, Bahçeci O, Özen Ş. Koagülasyon testlerinde optik ve mekanik pıhtı yöntemlerinin karşılaştırılması. Harran Üniversitesi Tıp Fakültesi Dergisi. 2019;16(1):13-6.

Harran Üniversitesi Tıp Fakültesi Dergisi  / Journal of Harran University Medical Faculty