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Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells

Year 2023, Volume: 7 Issue: 2, 151 - 156, 31.12.2023

Serkan Yelli Aslı Yıldırım Seçil Erden Tayhan Sema Bilgin İsa Gökçe

https://doi.org/10.32571/ijct.1249472

Abstract

Each tissue has its own anatomy and physiology. The deterioration of these structures by various effects (crushing, puncture, pathogens, etc.) is called as wound. The tissue exposed to these effects wants to gain the same function and anatomy. This phenomenon is called wound healing. Wound healing is a process that involves many reactions and cytokines. Since it is known that TGF-β3 and VEGF-A proteins which are among these cytokines, play an active role in wound healing stages. In the present study, it was decided to examine the wound healing effects of the non human gingival fibroblast cells comperatively. Wound healing process under the influence of these cytokines was performed by in vitro scratch analysis which is known as easy, reliable and cheap technique. In this context, firstly cell viability analysis was performed to determine the most effective dose for inducing cell proliferation. After this experimental step, gingival cells were incubated for 72 hours with suitable TGF-β3 and VEGF-A doses. As a result of the study, it was concluded that TGF-β3 and VEGF-A proteins are not cytotoxic and have a wound-healing effect on gingival fibroblast cells.

Keywords

TGF-β3 VEGF-A Gingival fibroblast cells wound healing.

Supporting Institution

Tokat Gaziosmanpaşa BAP Birimi

Project Number

2021/27

Thanks

The study is part of Serkan Yelli’s doctoral thesis that financially supported by Tokat Gaziosmanpaşa University Scientific Research Projects (2021/27).

References

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  • Ahmet, Ü; Aydın, H.; Tüzün, İ.S. Karşıdağ, Mühendislik Bilimleri ve Tasarım Dergi. 2014, 2(3):141-5.
  • Hormozi, M.; Assaei, R. Boroujeni, M.B. Biomedicine&Pharmacotherapy. 2017, 88:610-6.
  • Aktaş, Ş. ANKEM Derg. 2012, 26: 217-222
  • Barrientos, S.; Stojadinovic, O.; Golinko, M.S.; Brem, H.; Tomic‐Canic, M. Wound repair regen. 2008, 16(5):585-601.
  • Gence, H. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2008, 61(03):171-9.
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  • Monaco, JL.; Lawrence, W.T. Clinics in plastic surgery. 2003, 30(1):1-12.
  • Uzun, E. Diyabetik Ratlarda Topikal Vasküler Endotelyal Büyüme Faktörü (Vegf) Uygulamasının Yara Dokusundaki Oksidatif Olaylar Üzerine Etkisinin Araştırılması. Uzmanlık tezi. Gazi Üniversitesi 2013.
  • Witte, MB.; Barbul, A. Surgical Clinics of North America. 1997, 77(3):509-28.
  • Johnson, KE.; Wilgus, TA. Advances in wound care. 2014, 3(10):647-61.
  • Pappas, P.J.; You, R.; Rameshwar, P. J VascSurg. 1999, 30: 1129-45
  • Dvorak, L.F. Brown.; M, Detmar.; A.M. Dvorak. Am J Pathol. 1995, 146(5):1029–39
  • Chapple, C. C.; Kumar, R.K.; Hunter, N. J Oral PatholMed. 2000, 29:500–06.
  • Connolly, D.T.; Olander, J.V.; Heuvelman, D.; Nelson, R.; Monsell,R.; Siegel, N.; Haymor, B. L..E; Leingruber, R.; Feder, J. J BiolChem. 1989, 264(20):17–24.
  • Cao, L.; Jiao, X; Zuzga, D.S.; Liu, Y; Fong, D.M.; Young, D. NatGenet. 2004, 36(8):827–35.
  • Hoeben. A.; Landuyt. B.; Highley. M.S.; Wildiers. H.; Van Oosterom. A.T.; De Bruijn E.A. Pharmacol Rev. 2004,56 (4), 549–80
  • Dvorak, H.F.; Detmar, M.; Claffey, K.P.; Nagy, J.A.; Van de Water, L.; Senger, D.R.. Int. Arch. Allergy Immunol. 1995,107 (1-3), 233–235.
  • Valluru, M.; Staton, C.A.; Reed, M.W.; Brown, N.J. Front. Physiol. 2011, 2, 89.
  • Laverty, H.G.; Wakefield, L.M.; Occleston, N.L.; O’Kane, S.; Ferguson, M.W. Cytokine Growth Factor Rev. 2009, 20, 305–317.
  • Öztopalan, D.F.; Işık. R.; Durmuş. A.S. Dicle Üniv Vet Fak dergisi. 2017, 10:83-8.
  • Coşkun, Ö.; Uzun, G.; Dal, D.; Gülhane Tıp Dergi 2016, 58:207-28
  • Massague, J. Nat. Rev. Mol. Cell Biol. 2012, 13, 616–630.
  • Sprugel,.KH.; Mcpherson. J.M.; Clowes. A. Am J Pathol 1987, 129:601-13.
  • Kuduğ, C, H.; Erden, T. S.; Gökçe, İ. International Journal Of Peptide Research And Therapeutics, 2021. 27, pages1989–2001
  • Wongkhum, C.; Chotigeat, W.; Legat-Kedjarune, U. Mol Med Reb. 2018, Apr; 17(4):6100-6108.
  • Azadeh, J.; Xiujuan, Z.; ChangChih, L.; Ali, N. J Clin Lab Anal. 2017, Aug;54(6):633-48.
  • Ahmed, S.; Liu, CC.; Nawshad, A. Dev Biol. 2007, 309:193–207.
  • Nawshad, A.; Medici, D., Liu, C.C.; Hay, ED. J Cell Sci. 2007, 120:1646–1653
  • Kaartinen, V.; Cui, XM.; Heisterkamp, N.; Groffen, J.; Shuler, C.F. Dev Dyn. 1997, 209:255–260.
  • Lindhe, J.; Lang, N.P.; Berglundh, T.; Giannobile, W.V.; Sanz, M.; Wiley Blackwell; 2015.
  • Kohale, B. R.; Agrawal, A. A.; Raut, C.P. Journal of Indian Society of Periodontology, 2018, 22(5), 419.
  • Avani, Y.; Lerman, P.; Mint, S.; Kiviti, S. Develop Medicine and Child Neurology. 31:538-542, 1989.
  • Tayhan, E, S.; Bilgin, S.; Yıldırım, A.; Koç, E.; Ceylan, M. Chemistry & Biotmersity, 2022
  • İspir, E; İkiz, M; İnan, A.; Sünbül, A. B.; Tayhan, S. E.; Bilgin, S.; Köse, M.; Elmastaş, M. Journal of Molecular Structure. 2019, 1182, 63–71
  • Tayhan, S. E.; Bilgin, S.; Elmastaş, M. International Journal of Chemistry and Technology. 2018, 2, 16–19.
  • Zhu, Y.; Zhou, Ç.; Wang, Y. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 PMID: 15586711
  • Ling, Y .; Wayne, A.; Border, Y. H.; Nancy, A. Kidney Int. 2003, Sep;64(3):844-56.
  • Zhang, Y.; Liu, J.; Zou, T.; Qi, Y.; Yi, B.; Dissanayaka, W.L.; Zhang, C. Stem Cell Resther. 2021, May 10;12(1):281.
  • Prapulla, D.V.; Sujatha, P.B.; Pradeep, A.R.; J Periodontol. 2007, Sep;78(9):1783-7.
  • Nawshad, A.; Lagamba, D.; Hay, E.D. Arch Oral Biol. 2004, 49:675–689.

Year 2023, Volume: 7 Issue: 2, 151 - 156, 31.12.2023

Serkan Yelli Aslı Yıldırım Seçil Erden Tayhan Sema Bilgin İsa Gökçe

https://doi.org/10.32571/ijct.1249472

Abstract

Keywords

TGF-β3 VEGF-A Gingival fibroblast cells wound healing.

Project Number

2021/27

References

  • Werner, S.; Grose, R. Physiologica reviews. 2003, 83(3):835-70.
  • Ahmet, Ü; Aydın, H.; Tüzün, İ.S. Karşıdağ, Mühendislik Bilimleri ve Tasarım Dergi. 2014, 2(3):141-5.
  • Hormozi, M.; Assaei, R. Boroujeni, M.B. Biomedicine&Pharmacotherapy. 2017, 88:610-6.
  • Aktaş, Ş. ANKEM Derg. 2012, 26: 217-222
  • Barrientos, S.; Stojadinovic, O.; Golinko, M.S.; Brem, H.; Tomic‐Canic, M. Wound repair regen. 2008, 16(5):585-601.
  • Gence, H. Ankara Üniversitesi Tıp Fakültesi Mecmuası. 2008, 61(03):171-9.
  • Arthur, C.; Guyton, M., D.; John, E. Elsevier. 2006, p. 457-8.
  • Monaco, JL.; Lawrence, W.T. Clinics in plastic surgery. 2003, 30(1):1-12.
  • Uzun, E. Diyabetik Ratlarda Topikal Vasküler Endotelyal Büyüme Faktörü (Vegf) Uygulamasının Yara Dokusundaki Oksidatif Olaylar Üzerine Etkisinin Araştırılması. Uzmanlık tezi. Gazi Üniversitesi 2013.
  • Witte, MB.; Barbul, A. Surgical Clinics of North America. 1997, 77(3):509-28.
  • Johnson, KE.; Wilgus, TA. Advances in wound care. 2014, 3(10):647-61.
  • Pappas, P.J.; You, R.; Rameshwar, P. J VascSurg. 1999, 30: 1129-45
  • Dvorak, L.F. Brown.; M, Detmar.; A.M. Dvorak. Am J Pathol. 1995, 146(5):1029–39
  • Chapple, C. C.; Kumar, R.K.; Hunter, N. J Oral PatholMed. 2000, 29:500–06.
  • Connolly, D.T.; Olander, J.V.; Heuvelman, D.; Nelson, R.; Monsell,R.; Siegel, N.; Haymor, B. L..E; Leingruber, R.; Feder, J. J BiolChem. 1989, 264(20):17–24.
  • Cao, L.; Jiao, X; Zuzga, D.S.; Liu, Y; Fong, D.M.; Young, D. NatGenet. 2004, 36(8):827–35.
  • Hoeben. A.; Landuyt. B.; Highley. M.S.; Wildiers. H.; Van Oosterom. A.T.; De Bruijn E.A. Pharmacol Rev. 2004,56 (4), 549–80
  • Dvorak, H.F.; Detmar, M.; Claffey, K.P.; Nagy, J.A.; Van de Water, L.; Senger, D.R.. Int. Arch. Allergy Immunol. 1995,107 (1-3), 233–235.
  • Valluru, M.; Staton, C.A.; Reed, M.W.; Brown, N.J. Front. Physiol. 2011, 2, 89.
  • Laverty, H.G.; Wakefield, L.M.; Occleston, N.L.; O’Kane, S.; Ferguson, M.W. Cytokine Growth Factor Rev. 2009, 20, 305–317.
  • Öztopalan, D.F.; Işık. R.; Durmuş. A.S. Dicle Üniv Vet Fak dergisi. 2017, 10:83-8.
  • Coşkun, Ö.; Uzun, G.; Dal, D.; Gülhane Tıp Dergi 2016, 58:207-28
  • Massague, J. Nat. Rev. Mol. Cell Biol. 2012, 13, 616–630.
  • Sprugel,.KH.; Mcpherson. J.M.; Clowes. A. Am J Pathol 1987, 129:601-13.
  • Kuduğ, C, H.; Erden, T. S.; Gökçe, İ. International Journal Of Peptide Research And Therapeutics, 2021. 27, pages1989–2001
  • Wongkhum, C.; Chotigeat, W.; Legat-Kedjarune, U. Mol Med Reb. 2018, Apr; 17(4):6100-6108.
  • Azadeh, J.; Xiujuan, Z.; ChangChih, L.; Ali, N. J Clin Lab Anal. 2017, Aug;54(6):633-48.
  • Ahmed, S.; Liu, CC.; Nawshad, A. Dev Biol. 2007, 309:193–207.
  • Nawshad, A.; Medici, D., Liu, C.C.; Hay, ED. J Cell Sci. 2007, 120:1646–1653
  • Kaartinen, V.; Cui, XM.; Heisterkamp, N.; Groffen, J.; Shuler, C.F. Dev Dyn. 1997, 209:255–260.
  • Lindhe, J.; Lang, N.P.; Berglundh, T.; Giannobile, W.V.; Sanz, M.; Wiley Blackwell; 2015.
  • Kohale, B. R.; Agrawal, A. A.; Raut, C.P. Journal of Indian Society of Periodontology, 2018, 22(5), 419.
  • Avani, Y.; Lerman, P.; Mint, S.; Kiviti, S. Develop Medicine and Child Neurology. 31:538-542, 1989.
  • Tayhan, E, S.; Bilgin, S.; Yıldırım, A.; Koç, E.; Ceylan, M. Chemistry & Biotmersity, 2022
  • İspir, E; İkiz, M; İnan, A.; Sünbül, A. B.; Tayhan, S. E.; Bilgin, S.; Köse, M.; Elmastaş, M. Journal of Molecular Structure. 2019, 1182, 63–71
  • Tayhan, S. E.; Bilgin, S.; Elmastaş, M. International Journal of Chemistry and Technology. 2018, 2, 16–19.
  • Zhu, Y.; Zhou, Ç.; Wang, Y. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 PMID: 15586711
  • Ling, Y .; Wayne, A.; Border, Y. H.; Nancy, A. Kidney Int. 2003, Sep;64(3):844-56.
  • Zhang, Y.; Liu, J.; Zou, T.; Qi, Y.; Yi, B.; Dissanayaka, W.L.; Zhang, C. Stem Cell Resther. 2021, May 10;12(1):281.
  • Prapulla, D.V.; Sujatha, P.B.; Pradeep, A.R.; J Periodontol. 2007, Sep;78(9):1783-7.
  • Nawshad, A.; Lagamba, D.; Hay, E.D. Arch Oral Biol. 2004, 49:675–689.
There are 41 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Research Articles
Authors

Serkan Yelli 0000-0003-1938-1731

Aslı Yıldırım 0000-0001-5674-2095

Seçil Erden Tayhan 0000-0001-8473-5896

Sema Bilgin 0000-0001-5921-5434

İsa Gökçe 0000-0002-5023-9947

Project Number 2021/27
Early Pub Date January 8, 2024
Publication Date December 31, 2023
Published in Issue Year 2023 Volume: 7 Issue: 2

Cite

APA Yelli, S., Yıldırım, A., Erden Tayhan, S., Bilgin, S., et al. (2023). Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells. International Journal of Chemistry and Technology, 7(2), 151-156. https://doi.org/10.32571/ijct.1249472
AMA Yelli S, Yıldırım A, Erden Tayhan S, Bilgin S, Gökçe İ. Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells. Int. J. Chem. Technol. December 2023;7(2):151-156. doi:10.32571/ijct.1249472
Chicago Yelli, Serkan, Aslı Yıldırım, Seçil Erden Tayhan, Sema Bilgin, and İsa Gökçe. “Comparison of Thewound Healing Effects of Vascular Endothelial Growth Factor a (VEGF-A) and Transforming Growth Factor Beta 3 (TGF-β3) on Gingival Cells”. International Journal of Chemistry and Technology 7, no. 2 (December 2023): 151-56. https://doi.org/10.32571/ijct.1249472.
EndNote Yelli S, Yıldırım A, Erden Tayhan S, Bilgin S, Gökçe İ (December 1, 2023) Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells. International Journal of Chemistry and Technology 7 2 151–156.
IEEE S. Yelli, A. Yıldırım, S. Erden Tayhan, S. Bilgin, and İ. Gökçe, “Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells”, Int. J. Chem. Technol., vol. 7, no. 2, pp. 151–156, 2023, doi: 10.32571/ijct.1249472.
ISNAD Yelli, Serkan et al. “Comparison of Thewound Healing Effects of Vascular Endothelial Growth Factor a (VEGF-A) and Transforming Growth Factor Beta 3 (TGF-β3) on Gingival Cells”. International Journal of Chemistry and Technology 7/2 (December 2023), 151-156. https://doi.org/10.32571/ijct.1249472.
JAMA Yelli S, Yıldırım A, Erden Tayhan S, Bilgin S, Gökçe İ. Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells. Int. J. Chem. Technol. 2023;7:151–156.
MLA Yelli, Serkan et al. “Comparison of Thewound Healing Effects of Vascular Endothelial Growth Factor a (VEGF-A) and Transforming Growth Factor Beta 3 (TGF-β3) on Gingival Cells”. International Journal of Chemistry and Technology, vol. 7, no. 2, 2023, pp. 151-6, doi:10.32571/ijct.1249472.
Vancouver Yelli S, Yıldırım A, Erden Tayhan S, Bilgin S, Gökçe İ. Comparison of thewound healing effects of vascular endothelial growth factor a (VEGF-A) and transforming growth factor beta 3 (TGF-β3) on gingival cells. Int. J. Chem. Technol. 2023;7(2):151-6.

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