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ISOLATION AND CHARACTERISATION OF THE DEGRADATION POTENTIAL OF 2,2-DICHLOROPROPIONATE (2,2-DCP) BY BACILLUS AMYLOLIQUEFACIENS FROM GEBENG

Year 2022, Volume: 5 Issue: 2, 200 - 212, 15.08.2022
https://doi.org/10.38001/ijlsb.1064391

Abstract

Most halogenated compounds such as α-halocarboxylic acid (αHCA) are potential carcinogens that are toxic and widely liberated in both aquatic and terrestrial ecosystems. 2,2-dichloropropionate (2,2-DCP) is a synthetic halogenated compound commonly used in agricultural activities as an herbicide. This study is focused on the isolation and characterization of dehalogenase-producing bacteria capable of utilizing 2,2-dichloropropionate as the sole carbon and energy source from soil samples obtained from the industrial hub of Gebeng (Pahang), Malaysia. An enrichment culture technique supplemented with 2,2-DCP was used to isolate the bacteria. A pure culture strain designated as strain FDS grew well in media containing 20 mM 2,2-DCP at a temperature of 30˚C exhibiting a cell doubling time of 22.21hours and a maximum rate of chloride ion release of 0.345mmol/L suggesting this is the optimum concentration for growth. Based on microscopic observations, biochemical characteristics, and phylogenetic analysis of the 16S rRNA gene sequence, FDS was identified as Bacillus amyloliquefaciens with 98% homology. A literature survey revealed no report regarding the role of B. amyloliquefaciens on pesticide biodegradation as well as on 2,2-DCP, therefore the current isolate was designated Bacillus amyloliquefaciens FDS.

References

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  • Ashton, F. M., & Crafts, A. S. (1973). Mode of action of herbicides. Mode of action of herbicides.
  • Bergmann, J., & Sanik Jr, J. (1957). Determination of trace amounts of chlorine in naphtha. Analytical Chemistry, 29(2), 241-243. https://doi.org/10.1021/ac60122a018
  • Chen, X.-H., Scholz, R., Borriss, M., Junge, H., Mögel, G., Kunz, S., & Borriss, R. (2009). Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease. Journal of Biotechnology, 140(1), 38-44. https://doi.org/10.1016/j.jbiotec.2008.10.015
  • Chowdhury, S., Mishra, M., Adarsh, V., Mukherjee, A., Thakur, A. R., & Chaudhuri, S. R. (2008). Novel metal accumulator and protease secretor microbes from East Calcutta Wetland. American Journal of biochemistry and biotechnology, 3, 255-264. https://doi.org/10.3844/ajbbsp.2008.255.264
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  • Fulton, C. K., & Cooper, R. A. (2005). Catabolism of sulfamate by Mycobacterium sp. CF1. Environmental Microbiology, 7(3), 378-381. https://doi.org/10.1111/j.1462-2920.2005.00719.x
  • Hareland, W. A., Crawford, R. L., Chapman, P. J., & Dagley, S. (1975). Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. Journal of bacteriology, 121(1), 272-285. https://doi.org/10.1128/jb.121.1.272-285.1975
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  • Huyop, F., & Nemati, M. (2010). Properties of dehalogenase from Rhizobium sp. RC1. African Journal of Microbiology Research, 4(25), 2836-2847. https://doi.org/10.1186/s40064-016-2328-9
  • Kotan, R., Dikbas, N., & Bostan, H. (2009). Biological control of postharvest disease caused by Aspergillus flavus on stored lemon fruits. African Journal of Biotechnology, 8(2).
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  • Lamine Senghor, A., Liang, W.-J., & Ho, W.-C. (2007). Integrated control of Colletotrichum gloeosporioides on mango fruit in Taiwan by the combination of Bacillus s ubtilis and fruit bagging. Biocontrol science and technology, 17(8), 865-870. https://doi.org/10.1080/09583150701527409
  • Leclère, V., Béchet, M., Adam, A., Guez, J.-S., Wathelet, B., Ongena, M., Jacques, P. (2005). Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Applied and Environmental Microbiology, 71(8), 4577-4584. https://doi.org/10.1128/aem.71.8.4577-4584.2005
  • Medina‐Bellver, J. I., Marin, P., Delgado, A., Rodríguez‐Sánchez, A., Reyes, E., Ramos, J. L., & Marques, S. (2005). Evidence for in situ crude oil biodegradation after the Prestige oil spill. Environmental Microbiology, 7(6), 773-779. https://doi.org/10.1111/j.1462-2920.2005.00742.x
  • Nübel, U., Garcia-Pichel, F., & Muyzer, G. (1997). PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology, 63(8), 3327-3332. https://doi.org/10.1128/aem.63.8.3327-3332.1997
  • Piggot, P. J., & Hilbert, D. W. (2004). Sporulation of Bacillus subtilis. Current opinion in microbiology, 7(6), 579-586. https://doi.org/10.1016/j.mib.2004.10.001
  • Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  • Sathishkumar, M., Binupriya, A. R., Baik, S. H., & Yun, S. E. (2008). Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon-contaminated areas. CLEAN–Soil, Air, Water, 36(1), 92-96. https://doi.org/10.1002/clen.200700042
  • Tiago, I., Teixeira, I., Si1va, S., Chung, P., Veríssimo, A., & Manaia, C. M. (2004). C
  • Varsha, Y., Naga Deepthi, C., & Chenna, S. (2011). An emphasis on xenobiotic degradation in environmental cleanup. J Bioremed Biodegrad S, 11, 1-10. https://doi.org/10.4172/2155-6199.s11-001
  • Wang, Y., Xin, Y., Cao, X., & Xue, S. (2015). Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases. World Journal of Microbiology and Biotechnology, 31(4), 669-673. https://doi.org/10.1007/s11274-015-1817-2
  • Yang, Z., Guo, H., & Zhang, X. (2008). Study on the control of peach post-harvest diseases using Bacillus subtilis. China Fruits, 23, 35-38.
Year 2022, Volume: 5 Issue: 2, 200 - 212, 15.08.2022
https://doi.org/10.38001/ijlsb.1064391

Abstract

References

  • Aguilera, F., Méndez, J., Pásaro, E., & Laffon, B. (2010). Review on the effects of exposure to spilled oils on human health. Journal of Applied Toxicology, 30(4), 291-301. https://doi.org/10.1002/jat.1521
  • Alomar, D., Abdul Hamid, A. A., Khosrowabadi, E., Gicana, R. G., Lamis, R. J., Huyop, F., & Tengku Abdul Hamid, T. H. (2014). Molecular characterization of monochloroacetate-degrading Arthrobacter sp. Strain d2 isolated from Universiti Teknologi Malaysia agricultural area. Bioremediation Journal, 18(1), 12-19. https://doi.org/10.1080/10889868.2013.834867
  • Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids research, 25(17), 3389-3402. https://doi.org/10.1093/nar/25.17.3389
  • Ashton, F. M., & Crafts, A. S. (1973). Mode of action of herbicides. Mode of action of herbicides.
  • Bergmann, J., & Sanik Jr, J. (1957). Determination of trace amounts of chlorine in naphtha. Analytical Chemistry, 29(2), 241-243. https://doi.org/10.1021/ac60122a018
  • Chen, X.-H., Scholz, R., Borriss, M., Junge, H., Mögel, G., Kunz, S., & Borriss, R. (2009). Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease. Journal of Biotechnology, 140(1), 38-44. https://doi.org/10.1016/j.jbiotec.2008.10.015
  • Chowdhury, S., Mishra, M., Adarsh, V., Mukherjee, A., Thakur, A. R., & Chaudhuri, S. R. (2008). Novel metal accumulator and protease secretor microbes from East Calcutta Wetland. American Journal of biochemistry and biotechnology, 3, 255-264. https://doi.org/10.3844/ajbbsp.2008.255.264
  • Fravel, D. (2005). Commercialization and implementation of biocontrol 1. Annu. Rev. Phytopathol., 43, 337-359. https://doi.org/10.1146/annurev.phyto.43.032904.092924
  • Fulton, C. K., & Cooper, R. A. (2005). Catabolism of sulfamate by Mycobacterium sp. CF1. Environmental Microbiology, 7(3), 378-381. https://doi.org/10.1111/j.1462-2920.2005.00719.x
  • Hareland, W. A., Crawford, R. L., Chapman, P. J., & Dagley, S. (1975). Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. Journal of bacteriology, 121(1), 272-285. https://doi.org/10.1128/jb.121.1.272-285.1975
  • Holt, J., Krieg, N., Sneath, P., & Staley, J. (1994). Bergey’s manual of determinative bacteriology Williams and Wilkins. Baltimore, MD, 527. https://doi.org/10.1002/pauz.19900190410
  • Hughes, S. (1988). Microbial growth on 3-chloropropionic acid. University of Wales Institute of Science and Technology (UWIST).
  • Huyop, F., & Nemati, M. (2010). Properties of dehalogenase from Rhizobium sp. RC1. African Journal of Microbiology Research, 4(25), 2836-2847. https://doi.org/10.1186/s40064-016-2328-9
  • Kotan, R., Dikbas, N., & Bostan, H. (2009). Biological control of postharvest disease caused by Aspergillus flavus on stored lemon fruits. African Journal of Biotechnology, 8(2).
  • Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular biology and evolution, 33(7), 1870-1874.https://doi.org/10.1093/molbev/msw054
  • Lamine Senghor, A., Liang, W.-J., & Ho, W.-C. (2007). Integrated control of Colletotrichum gloeosporioides on mango fruit in Taiwan by the combination of Bacillus s ubtilis and fruit bagging. Biocontrol science and technology, 17(8), 865-870. https://doi.org/10.1080/09583150701527409
  • Leclère, V., Béchet, M., Adam, A., Guez, J.-S., Wathelet, B., Ongena, M., Jacques, P. (2005). Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Applied and Environmental Microbiology, 71(8), 4577-4584. https://doi.org/10.1128/aem.71.8.4577-4584.2005
  • Medina‐Bellver, J. I., Marin, P., Delgado, A., Rodríguez‐Sánchez, A., Reyes, E., Ramos, J. L., & Marques, S. (2005). Evidence for in situ crude oil biodegradation after the Prestige oil spill. Environmental Microbiology, 7(6), 773-779. https://doi.org/10.1111/j.1462-2920.2005.00742.x
  • Nübel, U., Garcia-Pichel, F., & Muyzer, G. (1997). PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology, 63(8), 3327-3332. https://doi.org/10.1128/aem.63.8.3327-3332.1997
  • Piggot, P. J., & Hilbert, D. W. (2004). Sporulation of Bacillus subtilis. Current opinion in microbiology, 7(6), 579-586. https://doi.org/10.1016/j.mib.2004.10.001
  • Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  • Sathishkumar, M., Binupriya, A. R., Baik, S. H., & Yun, S. E. (2008). Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon-contaminated areas. CLEAN–Soil, Air, Water, 36(1), 92-96. https://doi.org/10.1002/clen.200700042
  • Tiago, I., Teixeira, I., Si1va, S., Chung, P., Veríssimo, A., & Manaia, C. M. (2004). C
  • Varsha, Y., Naga Deepthi, C., & Chenna, S. (2011). An emphasis on xenobiotic degradation in environmental cleanup. J Bioremed Biodegrad S, 11, 1-10. https://doi.org/10.4172/2155-6199.s11-001
  • Wang, Y., Xin, Y., Cao, X., & Xue, S. (2015). Enhancement of L-2-haloacid dehalogenase expression in Pseudomonas stutzeri DEH138 based on the different substrate specificity between dehalogenase-producing bacteria and their dehalogenases. World Journal of Microbiology and Biotechnology, 31(4), 669-673. https://doi.org/10.1007/s11274-015-1817-2
  • Yang, Z., Guo, H., & Zhang, X. (2008). Study on the control of peach post-harvest diseases using Bacillus subtilis. China Fruits, 23, 35-38.
There are 26 citations in total.

Details

Primary Language English
Subjects Industrial Biotechnology
Journal Section Research Articles
Authors

Firdausi Aliyu 0000-0002-6400-1833

Aliyu Adamu

Roswanira Ab. Wahab

Fahrul Zaman Huyop

Publication Date August 15, 2022
Published in Issue Year 2022 Volume: 5 Issue: 2

Cite

EndNote Aliyu F, Adamu A, Ab. Wahab R, Zaman Huyop F (August 1, 2022) ISOLATION AND CHARACTERISATION OF THE DEGRADATION POTENTIAL OF 2,2-DICHLOROPROPIONATE (2,2-DCP) BY BACILLUS AMYLOLIQUEFACIENS FROM GEBENG. International Journal of Life Sciences and Biotechnology 5 2 200–212.



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