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Biological Activities of Wild Asparagus (Asparagus acutifolius L.)

Year 2018, Volume: 5 Issue: 3, 243 - 251, 15.09.2018
https://doi.org/10.21448/ijsm.458827

Abstract

Asparagus acutifolius L. (wild asparagus) is an edible plant species that is widely distributed throughout the Mediterranean. This study was designed to investigate the phenolic compounds, antioxidant, and cytotoxic activities of ethanol and water extracts of different parts (fruits and leaves) of A. acutifolius. The antioxidant activities of these extracts were analyzed using scavenging methods (DPPH and ABTS scavenging activity), the β-carotene/linoleic acid test system and the phosphomolybdenum method. Among the evaluations of the ethanol and water extracts of the different parts of A. acutifolius, the ethanol extract of the leaves was shown to have the highest amount of radical scavenging activities (DPPH, 0.383 mg/mL and ABTS, 0.145 mg/mL). Each extract of A. acutifolius exhibited strong antioxidant capacities with the β-carotene/Linoleic acid test system. For the phosphomolybdenum method, the antioxidant capacity of the extracts was in the range of 8.89-45.29 µg/mg and each extract exhibited high cytotoxic activities. The results will provide additional information for future studies on the biological activities of A. acutifolius, while also helping us to understand the importance of this species.

References

  • [1]. Pandey, M., Abidi, A.B., Singh, S.& Singh, R.P. (2006). Nutritional evaluation of leafy vegetable Paratha. Journal of Human Ecology, 19, 155. [2]. Kindl, M., Blazekovic, B., Bucar, F. & Vladimir-Knezevic, S. (2015). Antioxidant and anticholinesterase potential of Six Thymus Species. Evidence-Based Complementary and Alternative Medicine, 1-10. Doi: http://dx.doi.org/10.1155/2015/403950.
  • [3]. Bozzini, A. (1959). Revisione cito-sistematica del genere Asparagus L. I: Le specie di asparagus della flora italiana e chiave per la loro determinazione. Cariologia, 12, 199–264.
  • [4]. Fukushi, E., Onodera, S., Yamamori, A., Shiomi, N. & Kawabata, J. (2000). NMR analysis of triand tetrasaccharides from asparagus. Magnetic Resonance in Chemistry, 38, 1005–1011.
  • [5]. Kim, B. Y., Cui, Z. G., Lee, S. R., Kim, S. J., Kang, H. K., Lee, Y. K. & Park, D. B. (2009). Effects of Asparagus officinalis extracts on liver cell toxicity and ethanol metabolism. Journal of Food Science, 74, 204–208.
  • [6]. Makris, D. P. &– Rossiter, J. T. (2001). Domestic processing of onion bulbs (Allium cepa) and asparagus spears (Asparagus officinalis): Effect of flavonol content and antioxidant status. Journal of Agricultural and Food Chemistry, 49, 3216–3222.
  • [7]. Sautour M., Miyamoto T. & Dubois M.L. (2007). Steroidal saponins from Asparagus acutifolius. Phytochemistry, 68, 2554-2562.
  • [8]. Arcidiacono, S. & Pavone, P. (1994). Erbe spontanee commestibili del territorio etneo. Bollettino Accademia Gioenia di Scienze Naturali, 27, 346–481.
  • [9]. Venezia, A., Soressi, G.P., Falavigna, A. (1993). Aspetti relativi alla valorizzazione di specie di asparago spontanee in Italia. Agricoltura e Ricerca, 141, 41–48.
  • [10]. Di Maro, A., Pacifico, S., Fiorentino, A., Galasso, S., Gallicchio, M., Guida, V., Severino, V., Monaco, P. & Parente, A. (2013). Raviscanina wild asparagus (Asparagus acutifolius L.): A nutritionally valuable crop with antioxidant and antiproliferative properties. Food Research International, 53(1), 180-188.
  • [11]. Martins, D., Barros, L., Carvalho, A.M., & Ferreira, I.C.F.R. (2010). Nutritional and in vivo antioxidant properties of edible wild greens in Iberian Peninsula traditional diet. Food Chemistry, 125(2), 488-494.
  • [12]. Baytop T. (1999). Türkiye’de Bitkiler ile Tedavi. 2nd ed., Nobel Kitabevleri, İstanbul, 184-185.
  • [13]. Marc E.B., Nelly A., Annick D.D. & Frederic D. (2008). Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon. Journal of Ethnopharmacology, 120, 315-334.
  • [14]. Fenga C., Costa C., Caruso E., Raffa L., Alibrando C., Gangemi S., Docea A.O. & Tsatsakis A.M. (2016). Current evidence on the protective effect of dietary polyphenols on breast cancer., Farmacia, 64(1), 1-12.
  • [15]. Taskın, T. & Bitis, L. (2016). In vitro antioxidant activity of eight wild edible plants in Bursa province of Turkey. Farmacia, 64(5),706-711.
  • [16]. Ferrara, L., Dosi, R., Di Maro, A., Guida, V., Cefarelli, G., Pacifico, S., Mastellone, C., Fiorentino, A., Rosati, A., & Parente, A. (2011). Nutritional values, metabolic profile and radical scavenging capacities of wild asparagus (A. acutifolius L.). Journal of Food Composition and Analysis, 24, 326–333.
  • [17]. Kasture, S., Kasture, A., Ballero, M. & Maxia, A. (2009). Antioxidant, anti-inflammatory, and adaptogenic activity of Asparagus acutifolius extract. Oriental Pharmacy and Experimental Medicine, 9(1), 83-89.
  • [18]. Palfi, M., Jurković, Z., Ćosić, J., Tomić-Obrdalj, H., Jurković, V., Knežević, N. & Vrandečić, K. (2017). Total polyphenol content and antıoxıdant actıvıty of wild and cultivated asparagus ın croatıa. Portal Znanstventh Casopisa Republike Hrvatske, 23(1), 56-62.
  • [19]. Amin, I. & Tan, SH. (2002). Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition, 8(2), 167-177.
  • [20]. -Prieto, P., Pineda, M. & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337–341.
  • [21]. Meriga, B., Mopuri, R., & Krishna, T.M. (2012). Insecticidal antimicrobial and antioxidant activities of bulb extracts of Allium sativum. Asian Pacific Journal of Tropical Medicine, 5(5), 391-395
  • [22]. Shalaby, E.A., & Shanab, S.M.M. (2013). Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian Journal of Geo-Marine Sciences, 42(5), 556-564.
  • [23]. Slinkard, K. & Singleton VL. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28,49–55.
  • [24]. Arvouet-Grand, A., Vennat, B., Pourrat, A. & Legret, P. (1994). Standardization of a propolis extract and identification of the main constituents. Journal de pharmacie de Belgique, 49(6), 462-468.
  • [25]. Broadhurst, R.B., & Jones, W.T. (1978) Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture, 29(9), 788-794.
  • [26]. Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E. & McLaughlin, J.L. (1982). Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31-34.
  • [27]. Finney DJ. (1971). Probit Analysis. 3rd ed. Cambridge University Press, Cambridge.
  • [28]. Tepe B., Daferera D., Tepe A.S., Polissiou M. & Sokmen A. (2007). Antioxidant activity of the essential oil and various extracts of Nepeta flavida Hub.-Mor. from Turkey. Food Chemistry, 103,1358-1364.
  • [29]. Adıgüzel, A., Ozer, H., Sokmen, M., Gulluce, M., Sokmen, A., Kılıç, H., Sahin, F. & Baris, O. (2009). Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. Polish Journal of Microbiology, 58(1), 69-76.
  • [30]. Zengin, G. & Aktumsek, A. (2014). Investigation of antioxidant potentials of solvent extracts from different anatomical parts of Asphodeline anatolica E. Tuzlaci an endemic plant to Turkey. African Journal of Traditional Complementary, and Alternative Medicines, 11(2), 481–488.
  • [31]. Uysal, S., Zengin, G., & Aktümsek, A. (2016). Antioxidant properties and enzyme inhibitory effects of extracts from Mandragora autumnalis and its fatty acid composition. Marmara Pharmaceutical Journal, 20,144-151.
  • [32]. McLaughlin, J.L., Chang, C.J. & Smith, D.L. (1991). Bench-top bioassays for the discovery of bioactive natural products: an update. In:Rhaman AU. (Ed). Studies in Natural Products Chemistry, Elsevier, 383-409.
  • [33]. Krishnaraju, A.V., Rao, T.V.N., Sundararaju, D., Vanisree, M., Tsay, H.S. & Subbaraju, G.V. (2005). Assessment of bioactivity of Indian medicinal plants using Brine Shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3(2), 125-134.
  • [34]. Adoum, O.A., Dabo, N.T. & Fatobe, M.O. (1997). Bioactivities of some savanna plants in the brine shrimp lethality test and in vitro antimicrobial assay. International Journal of Pharmacognosy, 35(5), 334-337.
  • [35]. Mayorga, P., Perz, K.R., Cruz, S.M. & Caceres, A. (2010). Comparison of bioassays using the anostracan crustaceans. Artemiz salina and Thamnocephalus platyurus for plant extract toxicity screening. Revista Brasileira de Farmacognosia, 20(6), 897-903.
  • [36]. Oliva, M. de las M., Gallucci, N., Zygadlo J.A. & Demo, M.S. (2007). Cytotoxic activity of Argentinean essential oils on Artemia salina. Pharmaceutical Biology, 45(4), 259-262,
  • [37]. Svoboda, K.P. & Hampson, J.B. (1999). Bioactivity of essential oils of selected temperate aromatic plants: antibacterial, antioxidant, anti-Inflammatory and other related pharmacological activities, Plant Biology Department, SAC Auchincruive, Ayr, Scotland, UK., KA6 5HW.
  • [38]. Hossain, A.M., Ferdous, T., Salehuddin, S.M. & Das, A.K. (2009). In vitro cytotoxicity (LC50) of extracts obtained from the seeds of Zea mays. Asian Journal of Food and Agro-Industry, 2(3), 336-34.

Biological Activities of Wild Asparagus (Asparagus acutifolius L.)

Year 2018, Volume: 5 Issue: 3, 243 - 251, 15.09.2018
https://doi.org/10.21448/ijsm.458827

Abstract

Asparagus acutifolius L. (wild asparagus)
is an edible plant species that is widely distributed throughout the
Mediterranean. This study was designed to investigate the phenolic compounds,
antioxidant, and cytotoxic activities of ethanol and water extracts of
different parts (fruits and leaves) of A.
acutifolius.
The antioxidant activities of these extracts were analyzed
using scavenging methods (DPPH and ABTS scavenging activity), the
β-carotene/linoleic acid test system and the phosphomolybdenum method. Among
the evaluations of the ethanol and water extracts of the different parts of A. acutifolius, the ethanol extract of
the leaves was shown to have the highest amount of radical scavenging
activities (DPPH, 0.383 mg/mL and ABTS, 0.145 mg/mL). Each extract of A. acutifolius exhibited strong
antioxidant capacities with the β-carotene/Linoleic acid test system. For the
phosphomolybdenum method, the antioxidant capacity of the extracts was in the
range of 8.89-45.29 µg/mg and each extract exhibited high cytotoxic activities.
The results will provide additional information for future studies on the
biological activities of A. acutifolius, while
also helping us to understand the importance of this species.

References

  • [1]. Pandey, M., Abidi, A.B., Singh, S.& Singh, R.P. (2006). Nutritional evaluation of leafy vegetable Paratha. Journal of Human Ecology, 19, 155. [2]. Kindl, M., Blazekovic, B., Bucar, F. & Vladimir-Knezevic, S. (2015). Antioxidant and anticholinesterase potential of Six Thymus Species. Evidence-Based Complementary and Alternative Medicine, 1-10. Doi: http://dx.doi.org/10.1155/2015/403950.
  • [3]. Bozzini, A. (1959). Revisione cito-sistematica del genere Asparagus L. I: Le specie di asparagus della flora italiana e chiave per la loro determinazione. Cariologia, 12, 199–264.
  • [4]. Fukushi, E., Onodera, S., Yamamori, A., Shiomi, N. & Kawabata, J. (2000). NMR analysis of triand tetrasaccharides from asparagus. Magnetic Resonance in Chemistry, 38, 1005–1011.
  • [5]. Kim, B. Y., Cui, Z. G., Lee, S. R., Kim, S. J., Kang, H. K., Lee, Y. K. & Park, D. B. (2009). Effects of Asparagus officinalis extracts on liver cell toxicity and ethanol metabolism. Journal of Food Science, 74, 204–208.
  • [6]. Makris, D. P. &– Rossiter, J. T. (2001). Domestic processing of onion bulbs (Allium cepa) and asparagus spears (Asparagus officinalis): Effect of flavonol content and antioxidant status. Journal of Agricultural and Food Chemistry, 49, 3216–3222.
  • [7]. Sautour M., Miyamoto T. & Dubois M.L. (2007). Steroidal saponins from Asparagus acutifolius. Phytochemistry, 68, 2554-2562.
  • [8]. Arcidiacono, S. & Pavone, P. (1994). Erbe spontanee commestibili del territorio etneo. Bollettino Accademia Gioenia di Scienze Naturali, 27, 346–481.
  • [9]. Venezia, A., Soressi, G.P., Falavigna, A. (1993). Aspetti relativi alla valorizzazione di specie di asparago spontanee in Italia. Agricoltura e Ricerca, 141, 41–48.
  • [10]. Di Maro, A., Pacifico, S., Fiorentino, A., Galasso, S., Gallicchio, M., Guida, V., Severino, V., Monaco, P. & Parente, A. (2013). Raviscanina wild asparagus (Asparagus acutifolius L.): A nutritionally valuable crop with antioxidant and antiproliferative properties. Food Research International, 53(1), 180-188.
  • [11]. Martins, D., Barros, L., Carvalho, A.M., & Ferreira, I.C.F.R. (2010). Nutritional and in vivo antioxidant properties of edible wild greens in Iberian Peninsula traditional diet. Food Chemistry, 125(2), 488-494.
  • [12]. Baytop T. (1999). Türkiye’de Bitkiler ile Tedavi. 2nd ed., Nobel Kitabevleri, İstanbul, 184-185.
  • [13]. Marc E.B., Nelly A., Annick D.D. & Frederic D. (2008). Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon. Journal of Ethnopharmacology, 120, 315-334.
  • [14]. Fenga C., Costa C., Caruso E., Raffa L., Alibrando C., Gangemi S., Docea A.O. & Tsatsakis A.M. (2016). Current evidence on the protective effect of dietary polyphenols on breast cancer., Farmacia, 64(1), 1-12.
  • [15]. Taskın, T. & Bitis, L. (2016). In vitro antioxidant activity of eight wild edible plants in Bursa province of Turkey. Farmacia, 64(5),706-711.
  • [16]. Ferrara, L., Dosi, R., Di Maro, A., Guida, V., Cefarelli, G., Pacifico, S., Mastellone, C., Fiorentino, A., Rosati, A., & Parente, A. (2011). Nutritional values, metabolic profile and radical scavenging capacities of wild asparagus (A. acutifolius L.). Journal of Food Composition and Analysis, 24, 326–333.
  • [17]. Kasture, S., Kasture, A., Ballero, M. & Maxia, A. (2009). Antioxidant, anti-inflammatory, and adaptogenic activity of Asparagus acutifolius extract. Oriental Pharmacy and Experimental Medicine, 9(1), 83-89.
  • [18]. Palfi, M., Jurković, Z., Ćosić, J., Tomić-Obrdalj, H., Jurković, V., Knežević, N. & Vrandečić, K. (2017). Total polyphenol content and antıoxıdant actıvıty of wild and cultivated asparagus ın croatıa. Portal Znanstventh Casopisa Republike Hrvatske, 23(1), 56-62.
  • [19]. Amin, I. & Tan, SH. (2002). Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition, 8(2), 167-177.
  • [20]. -Prieto, P., Pineda, M. & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337–341.
  • [21]. Meriga, B., Mopuri, R., & Krishna, T.M. (2012). Insecticidal antimicrobial and antioxidant activities of bulb extracts of Allium sativum. Asian Pacific Journal of Tropical Medicine, 5(5), 391-395
  • [22]. Shalaby, E.A., & Shanab, S.M.M. (2013). Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian Journal of Geo-Marine Sciences, 42(5), 556-564.
  • [23]. Slinkard, K. & Singleton VL. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28,49–55.
  • [24]. Arvouet-Grand, A., Vennat, B., Pourrat, A. & Legret, P. (1994). Standardization of a propolis extract and identification of the main constituents. Journal de pharmacie de Belgique, 49(6), 462-468.
  • [25]. Broadhurst, R.B., & Jones, W.T. (1978) Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture, 29(9), 788-794.
  • [26]. Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E. & McLaughlin, J.L. (1982). Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31-34.
  • [27]. Finney DJ. (1971). Probit Analysis. 3rd ed. Cambridge University Press, Cambridge.
  • [28]. Tepe B., Daferera D., Tepe A.S., Polissiou M. & Sokmen A. (2007). Antioxidant activity of the essential oil and various extracts of Nepeta flavida Hub.-Mor. from Turkey. Food Chemistry, 103,1358-1364.
  • [29]. Adıgüzel, A., Ozer, H., Sokmen, M., Gulluce, M., Sokmen, A., Kılıç, H., Sahin, F. & Baris, O. (2009). Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. Polish Journal of Microbiology, 58(1), 69-76.
  • [30]. Zengin, G. & Aktumsek, A. (2014). Investigation of antioxidant potentials of solvent extracts from different anatomical parts of Asphodeline anatolica E. Tuzlaci an endemic plant to Turkey. African Journal of Traditional Complementary, and Alternative Medicines, 11(2), 481–488.
  • [31]. Uysal, S., Zengin, G., & Aktümsek, A. (2016). Antioxidant properties and enzyme inhibitory effects of extracts from Mandragora autumnalis and its fatty acid composition. Marmara Pharmaceutical Journal, 20,144-151.
  • [32]. McLaughlin, J.L., Chang, C.J. & Smith, D.L. (1991). Bench-top bioassays for the discovery of bioactive natural products: an update. In:Rhaman AU. (Ed). Studies in Natural Products Chemistry, Elsevier, 383-409.
  • [33]. Krishnaraju, A.V., Rao, T.V.N., Sundararaju, D., Vanisree, M., Tsay, H.S. & Subbaraju, G.V. (2005). Assessment of bioactivity of Indian medicinal plants using Brine Shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3(2), 125-134.
  • [34]. Adoum, O.A., Dabo, N.T. & Fatobe, M.O. (1997). Bioactivities of some savanna plants in the brine shrimp lethality test and in vitro antimicrobial assay. International Journal of Pharmacognosy, 35(5), 334-337.
  • [35]. Mayorga, P., Perz, K.R., Cruz, S.M. & Caceres, A. (2010). Comparison of bioassays using the anostracan crustaceans. Artemiz salina and Thamnocephalus platyurus for plant extract toxicity screening. Revista Brasileira de Farmacognosia, 20(6), 897-903.
  • [36]. Oliva, M. de las M., Gallucci, N., Zygadlo J.A. & Demo, M.S. (2007). Cytotoxic activity of Argentinean essential oils on Artemia salina. Pharmaceutical Biology, 45(4), 259-262,
  • [37]. Svoboda, K.P. & Hampson, J.B. (1999). Bioactivity of essential oils of selected temperate aromatic plants: antibacterial, antioxidant, anti-Inflammatory and other related pharmacological activities, Plant Biology Department, SAC Auchincruive, Ayr, Scotland, UK., KA6 5HW.
  • [38]. Hossain, A.M., Ferdous, T., Salehuddin, S.M. & Das, A.K. (2009). In vitro cytotoxicity (LC50) of extracts obtained from the seeds of Zea mays. Asian Journal of Food and Agro-Industry, 2(3), 336-34.
There are 37 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Arzu Kaska 0000-0002-0166-1818

Nahide Deniz This is me

Ramazan Mammadov

Publication Date September 15, 2018
Submission Date May 17, 2018
Published in Issue Year 2018 Volume: 5 Issue: 3

Cite

APA Kaska, A., Deniz, N., & Mammadov, R. (2018). Biological Activities of Wild Asparagus (Asparagus acutifolius L.). International Journal of Secondary Metabolite, 5(3), 243-251. https://doi.org/10.21448/ijsm.458827
International Journal of Secondary Metabolite

e-ISSN: 2148-6905