Research Article
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Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers

Year 2024, Volume: 8 Issue: 1, 88 - 93, 25.03.2024

Muharrem Yılmaz Ahsen Işık Özgüven Yıldız Aka Kaçar

https://doi.org/10.31015/jaefs.2024.1.10

Abstract

This study defines the genetic characterization of 16 hazelnut varieties and 64 genotypes. SSR method was used in molecular characterization studies. 18 SSR primers were used. In the SSR method, 118 bands were obtained, and 115 were polymorphic. The similarity coefficient in overall genotypes were between 0.12 and 0.98 in SSR andthe polymorphism rate of the primer pairs used was calculated as 98.0%. The lowest polymorphism information content value (0.542) was obtained from the CAC- C 028 locus, and the highest polymorphism information content value (0.987) was obtained from the CAC- B 113 locus. The average polymorphism information content value was 0.786. The total discrimination power values of SSR loci were determined as 17.98; the lowest (0.748) discrimination power value was obtained from the CAC- B 020 locus, while the highest (1.404) discrimination power value was obtained from the CAC- A 024 b locus. The genotypes’clustering positions across the dendograms were essentially correlated with their geographic origins. This result shows that genetic origin plays an important role in terms of classification of genotypes in hazelnut.

Keywords

Hazelnut Molecular characterization SSR

References

  • Ayfer, M., Uzun, A. ve Baş, F., (1986). Türk Fındık Çeşitleri. Karadeniz Bölgesi Fındık İhracatçılar Birliği Yayınları, Ankara, 95 s(in Turkish).
  • Bassil, N.V., Botta, R., Mehlenbacher, S.A. (2003). Microsatellite Markers Of The European Hazelnut. Hortscience. 38(5):Abstract P.740-741.
  • Boccaccı, P., Akkak, A., Botta, R., (2006). DNA Typing and Genetic Relations Among European Hazelnut (Corylus avellena) Cultivars Using Microsatellite Markers. Genome. 49. 598.
  • Doyle, J.J., Doyle, J.J., (1990). Isolation of Plant DNA from Fresh Tissue. Focus. 12: 13 – 15.
  • Fiore, M C., Marchese A., Mauceri A., Digangl I., Scialabba, A.(2022). Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm. Plants 2022, 11, 631. https://doi.org/10.3390
  • Gökırmak, T., Mahlenbacher, S.A., Bassil, N.V., (2008). Characterization of European Hazelnut ( Corylus avellena) Cultivars Using SSR Markers. Gent Resour Crop Evol. ( doi:10.1007/s10722- 088-9352-8)
  • HRI, (2024). Hazelnut Research Institute. https://arastirma.tarimorman.gov.tr/findik/Sayfalar/EN/AnaSayfa.aspx (accessed date 07.01.2024)
  • İslam, A., ve Özgüven, A.I. (1997).Türkiye’de Fındık Yetiştiriciliği. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 12 (4): 165 – 174 (in Turkish).
  • İslam, A., (2000). Clonal selection in Turkish hazelnut cultivars grown in Ordu. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
  • Kaçar Aka, Y. (2001). Classification of some sweet (p. avium L. ) and sourcherry (p. cerasus L. ) varieties and types grown in Turkey by using DNA fingerprinting. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
  • Köksal, A. İ., (2002). Türk Fındık Çeşitleri. Fındık Tanıtım Grubu Yayını, 136 s (in Turkish).
  • Mehlenbacher, S. A., Brown, R.N., Nouhra, E.R., Gökırmak, T., Bassil, N.V. and Kusiak, T.L.,(2006). A Genetic Linkage Map For Hazelnut (Corylus avellena) Based on RAPD and SSR Markers. Genome; Feb 2006; 49,2.
  • Okay, A.N., Kaya, A., Küçük, V.Y., Ve Küçük A., (1986). Fındık Tarımı. TKB, Teşkilatlanma ve Destekleme Genel Müd., Yayın No: Genel 142, Tedgem 12, Ankara, 85s (in Turkish).
  • Özbek, S., (1978). Özel Meyvecilik (Kışın Yaprağını Döken Meyve Türleri). Çukurova Üniversitesi Ziraat Fakültesi Yayınları, 486 s (in Turkish).
  • Özcan. S., Gürel. E.,Babaoğlu, M., (2001). Bitki Biyoteknolojisi II. Genetik Mühendisliği ve Uygulamaları, ISBN: 975-6652-05-5 (in Turkish).
  • Prevost, A., Wilkinson, M. J., (1999). A New System of Comprasing PCR Primers Applied to ISSR Fingerprinting of Potato Accessions. Theoretical and Applied Genetics, 98:107–112.
  • Rohlf, J. F., (2004). NTSYS-pc: 2.11 Numerical Taxonomy and Multivariate Analysis System. Exeter Software, N.Y.
  • Smith, J. S. C., Chin, E. C. L., Shu, H., Smith, O. S., Wall, S. J., Senior, M. L., Mitchel, S. E., Kresorich, S., Tiegle, J., (1997). An Evaloution 329 of the Utility of SSR Loci as Molecular Marker in Maize (Zea mays): Comparisons with Data from RFLP and Pedigree. Theoretical and Applied Genetics, 95:163–173.
  • Yang Z., Zhao T., Liang L., Jiang L., Wang L., Hou S., Ma Q.(2023). Genetic Analysis of the Cultivars of Ping’ou Hybrid Hazelnut (C. heterophylla Fisch. × C. avellana L.) in China Based on SSR Markers. Forests.DOI:10.3390/f14071405.

Year 2024, Volume: 8 Issue: 1, 88 - 93, 25.03.2024

Muharrem Yılmaz Ahsen Işık Özgüven Yıldız Aka Kaçar

https://doi.org/10.31015/jaefs.2024.1.10

Abstract

References

  • Ayfer, M., Uzun, A. ve Baş, F., (1986). Türk Fındık Çeşitleri. Karadeniz Bölgesi Fındık İhracatçılar Birliği Yayınları, Ankara, 95 s(in Turkish).
  • Bassil, N.V., Botta, R., Mehlenbacher, S.A. (2003). Microsatellite Markers Of The European Hazelnut. Hortscience. 38(5):Abstract P.740-741.
  • Boccaccı, P., Akkak, A., Botta, R., (2006). DNA Typing and Genetic Relations Among European Hazelnut (Corylus avellena) Cultivars Using Microsatellite Markers. Genome. 49. 598.
  • Doyle, J.J., Doyle, J.J., (1990). Isolation of Plant DNA from Fresh Tissue. Focus. 12: 13 – 15.
  • Fiore, M C., Marchese A., Mauceri A., Digangl I., Scialabba, A.(2022). Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm. Plants 2022, 11, 631. https://doi.org/10.3390
  • Gökırmak, T., Mahlenbacher, S.A., Bassil, N.V., (2008). Characterization of European Hazelnut ( Corylus avellena) Cultivars Using SSR Markers. Gent Resour Crop Evol. ( doi:10.1007/s10722- 088-9352-8)
  • HRI, (2024). Hazelnut Research Institute. https://arastirma.tarimorman.gov.tr/findik/Sayfalar/EN/AnaSayfa.aspx (accessed date 07.01.2024)
  • İslam, A., ve Özgüven, A.I. (1997).Türkiye’de Fındık Yetiştiriciliği. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 12 (4): 165 – 174 (in Turkish).
  • İslam, A., (2000). Clonal selection in Turkish hazelnut cultivars grown in Ordu. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
  • Kaçar Aka, Y. (2001). Classification of some sweet (p. avium L. ) and sourcherry (p. cerasus L. ) varieties and types grown in Turkey by using DNA fingerprinting. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
  • Köksal, A. İ., (2002). Türk Fındık Çeşitleri. Fındık Tanıtım Grubu Yayını, 136 s (in Turkish).
  • Mehlenbacher, S. A., Brown, R.N., Nouhra, E.R., Gökırmak, T., Bassil, N.V. and Kusiak, T.L.,(2006). A Genetic Linkage Map For Hazelnut (Corylus avellena) Based on RAPD and SSR Markers. Genome; Feb 2006; 49,2.
  • Okay, A.N., Kaya, A., Küçük, V.Y., Ve Küçük A., (1986). Fındık Tarımı. TKB, Teşkilatlanma ve Destekleme Genel Müd., Yayın No: Genel 142, Tedgem 12, Ankara, 85s (in Turkish).
  • Özbek, S., (1978). Özel Meyvecilik (Kışın Yaprağını Döken Meyve Türleri). Çukurova Üniversitesi Ziraat Fakültesi Yayınları, 486 s (in Turkish).
  • Özcan. S., Gürel. E.,Babaoğlu, M., (2001). Bitki Biyoteknolojisi II. Genetik Mühendisliği ve Uygulamaları, ISBN: 975-6652-05-5 (in Turkish).
  • Prevost, A., Wilkinson, M. J., (1999). A New System of Comprasing PCR Primers Applied to ISSR Fingerprinting of Potato Accessions. Theoretical and Applied Genetics, 98:107–112.
  • Rohlf, J. F., (2004). NTSYS-pc: 2.11 Numerical Taxonomy and Multivariate Analysis System. Exeter Software, N.Y.
  • Smith, J. S. C., Chin, E. C. L., Shu, H., Smith, O. S., Wall, S. J., Senior, M. L., Mitchel, S. E., Kresorich, S., Tiegle, J., (1997). An Evaloution 329 of the Utility of SSR Loci as Molecular Marker in Maize (Zea mays): Comparisons with Data from RFLP and Pedigree. Theoretical and Applied Genetics, 95:163–173.
  • Yang Z., Zhao T., Liang L., Jiang L., Wang L., Hou S., Ma Q.(2023). Genetic Analysis of the Cultivars of Ping’ou Hybrid Hazelnut (C. heterophylla Fisch. × C. avellana L.) in China Based on SSR Markers. Forests.DOI:10.3390/f14071405.
There are 19 citations in total.

Details

Primary Language English
Subjects Pomology and Treatment
Journal Section Research Articles
Authors

Muharrem Yılmaz 0000-0002-3939-9907

Ahsen Işık Özgüven 0000-0001-8755-9728

Yıldız Aka Kaçar 0000-0001-5314-7952

Publication Date March 25, 2024
Submission Date February 7, 2024
Acceptance Date March 4, 2024
Published in Issue Year 2024 Volume: 8 Issue: 1

Cite

APA Yılmaz, M., Işık Özgüven, A., & Aka Kaçar, Y. (2024). Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers. International Journal of Agriculture Environment and Food Sciences, 8(1), 88-93. https://doi.org/10.31015/jaefs.2024.1.10
AMA Yılmaz M, Işık Özgüven A, Aka Kaçar Y. Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers. int. j. agric. environ. food sci. March 2024;8(1):88-93. doi:10.31015/jaefs.2024.1.10
Chicago Yılmaz, Muharrem, Ahsen Işık Özgüven, and Yıldız Aka Kaçar. “Molecular Characterization of Turkish Hazelnut Cultivars and Genotypes Using SSR Markers”. International Journal of Agriculture Environment and Food Sciences 8, no. 1 (March 2024): 88-93. https://doi.org/10.31015/jaefs.2024.1.10.
EndNote Yılmaz M, Işık Özgüven A, Aka Kaçar Y (March 1, 2024) Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers. International Journal of Agriculture Environment and Food Sciences 8 1 88–93.
IEEE M. Yılmaz, A. Işık Özgüven, and Y. Aka Kaçar, “Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers”, int. j. agric. environ. food sci., vol. 8, no. 1, pp. 88–93, 2024, doi: 10.31015/jaefs.2024.1.10.
ISNAD Yılmaz, Muharrem et al. “Molecular Characterization of Turkish Hazelnut Cultivars and Genotypes Using SSR Markers”. International Journal of Agriculture Environment and Food Sciences 8/1 (March 2024), 88-93. https://doi.org/10.31015/jaefs.2024.1.10.
JAMA Yılmaz M, Işık Özgüven A, Aka Kaçar Y. Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers. int. j. agric. environ. food sci. 2024;8:88–93.
MLA Yılmaz, Muharrem et al. “Molecular Characterization of Turkish Hazelnut Cultivars and Genotypes Using SSR Markers”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 1, 2024, pp. 88-93, doi:10.31015/jaefs.2024.1.10.
Vancouver Yılmaz M, Işık Özgüven A, Aka Kaçar Y. Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers. int. j. agric. environ. food sci. 2024;8(1):88-93.
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