Research Article
Effect of different genetic manipulations on the production of xylanase enzyme in Streptomyces strains
Abstract
Soil samples were collected from five different areas of University of Çukurova and 3 Streptomyces strains were isolated. The xylanase activity (at the end of the seventh day) of S40, S47 and S48 strains were found as 50,92U/mL, 49,51U/mL and 47,79U/mL respectively. After doing the protoplast fusion among these strains which have the highest xylanase activity, 9 strains which have different colony morphologies, were selected. The xylanase activity of (40-48) R2 and (47-48) R3 strains were increased in comparison with their parents, by 25,9% and 31,9% respectively. This study also indicated that the enzyme activity can be improved by protoplast fusion technique
Keywords
Project Number
FEF2009D20
References
- Refernas1. Abdel-Aziz, M. S., Talkhan, F. N., Fadel, M., AbouZied, A. A., and Abdel-Razik, A. S. (2011). Improvement of xylanase production from Streptomyces pseudogriseolus via UV mutagenesis. Australian Journal of Basic and Applied Sciences, 5(5), 1045-1050. Refernas2. Antonopoulos, V., Hernandez, M., Arias, M., Mavrakos, E., and Ball, A. (2001). The use of extracellular enzymes from Streptomyces albus ATCC 3005 for the bleaching of eucalyptus kraft pulp. Applied microbiology and biotechnology, 57(1-2), 92-97. Refernas3 Aretz, W., Koller, K. P., and Riess, G. (1989). Proteolytic enzymes from recombinant Streptomyces lividans TK24. FEMS Microbiology Letters, 65(1-2), 31-35. Refernas4 Aygan, A., 2008. Halofilik Bacillus sp. izolasyonu, amilaz selülaz ve ksilanaz enzimlerinin üretimi, karakterizasyonu ve biyoteknolojik uygulamalarda kullanılabilirliği. Ç.Ü. Fen Bil. Enst.Fakültesi. Adana, Türkiye. 186s. Refernas5 Bailey, M. J., Biely, P., and Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270. Refernas6 Bajaj, B. K., Razdan, K., & Sharma, A. (2010). Thermoactive alkali-stable xylanase production from a newly isolated Streptomyces sp. SU 9. Indian Journal of Chemical Tecnology. Vol:17 pp. 375-380. Refernas7 Baltz, R. H. (1978). Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. Microbiology, 107(1), 93-102. Refernas8 Bandlish, R. K., Michael Hess, J., Epting, K. L., Vieille, C., and Kelly, R. M. (2002). Glucose‐to‐fructose conversion at high temperatures with xylose (glucose) isomerases from Streptomyces murinus and two hyperthermophilic Thermotoga species. Biotechnology and Bioengineering, 80(2), 185-194. Refernas9 Bhosale, H. J., Sukalkar, S. R., Uzma, S. M. Z., & Kadam, T. A. (2011). Production of xylanase by Streptomyces rameus grown on agricultural wastes. Biotechnol Bioinf Bioeng, 1(4), 505-512. Refernas10 Chun, J., Youn, H. D., Yim, Y. I., Lee, H., Kim, M. Y., Hah, Y. C., & Kang, S. O. (1997). Streptomyces seoulensis sp. nov. International Journal of Systematic and Evolutionary Microbiology, 47(2), 492-498. Refernas11 .Crawford, D. L., Lynch, J. M., Whipps, J. M., & Ousley, M. A. (1993). Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Applied Environmental Microbiology. 59(11), 3899-3905. Refernas12 Ding, C. H., Jiang, Z. Q., Li, X. T., Li, L. T., and Kusakabe, I. (2004). High activity xylanase production by Streptomyces olivaceoviridis E-86. World Journal of Microbiology and Biotechnology, 20(1), 7-10. Refernas13 Gold, M. H., Cheng, T. M., & Alic, M. (1983). Formation, fusion, and regeneration of protoplasts from wild-type and auxotrophic strains of the white rot basidiomycete Phanerochaete chrysosporium. Appl. Environ. Microbiol., 46(1), 260-263. Refernas14 Kaneko, T., Saito, K., Kawamura, Y., & Takahashi, S. (2001). Molecular cloning of acid-stable glucose isomerase gene from Streptomyces olivaceoviridis E-86 by a simple two-step PCR method, and its expression in Escherichia coli. Bioscience, Biotechnology, and Biochemistry, 65(5), 1054-1062. Refernas15 Kansoh, A. L., & Nagieb, Z. A. (2004). Xylanase and mannanase enzymes from Streptomyces galbus NR and their use in biobleaching of softwood kraft pulp. Antonie Van Leeuwenhoek, 85(2), 103-114. Refernas16 Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F., & Hopwood, D. A. (2000). Practical streptomyces genetics (Vol. 291). Norwich: John Innes Foundation. Norwich, UK, 613p. Refernas17 Lampel, J. S., & Strohl, W. R. (1986). Transformation and transfection of anthracycline-producing streptomycetes. Applied Environmental Microbiology, 51(1), 126-131. Refernas18 Lelong, C., Chevallet, M., Luche,S. and Rabilloud, T. (2009). Silver staining of proteins in 2DE gels. Two-Dimensional Electrophoresis protocols ( pp. 339-350). Humana Press. Refernas19 Li, W., Lanoot, B., Zhang, Y., Vancanneyt, M., Swings, J., & Liu, Z. (2002). Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. International Journal of Systematic and Evolutionary Microbiology, 52(5), 1629-1633. Refernas20 Li, X., Sun, B., Zhao, J., Lv, Y., Song, H., Li, E., & Zhu, Y. (2011). Production and improved bleaching abilities of a thermostable xylanase from a newly isolated Streptomyces chartreusis strain. African journal of Biotechnology, 10(64), 14132-14142. Refernas21 Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193, 265-275. Refernas22 Meyers, P. R., Porter, D. S., Omorogie, C., Pule, J. M., and Kwetane, T. (2003). Streptomyces speibonae sp. nov., a novel streptomycete with blue substrate mycelium isolated from South African soil. International Journal of Systematic and Evolutionary Microbiology, 53(3), 801-805. Refernas23 Nadia, H., El-Nassar, A., Amal, M. A., and Abeer, A. K. (2010). Xylanase production by Streptomyces lividans (NRC) and it’s application on waste paper. Australian Journal of Basic and Applied Sciences, 4(6), 1358-1368. Refernas24 Nascimento, R. P., Coelho, R. R. R., Marques, S., Alves, L., Gırio, F. M., Bon, E. P. S., & Amaral-Collaço, M. T. (2002). Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme and Microbial Technology, 31(4), 549-555. Refernas25 Pigac, J., & Schrempf, H. (1995). A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation. Applied Environmental Microbiology, 61(1), 352-356. Refernas26 Raykovska, V., Dolashka-Angelova, P., Paskaleva, D., Stoeva, S., Abashev, J., Kirkov, L., & Voelter, W. (2001). Isolation and characterization of a xylose-glucose isomerase from a new strain Streptomyces thermovulgaris 127, var. 7-86. Biochemistry and Cell Biology, 79(2), 195-205. Refernas27 Rifaat, H. M., Nagieb, Z. A., & Ahmed, Y. M. (2006). Production of xylanases by Streptomyces species and their bleaching effect on rice straw pulp. Applied Ecology and Environmental Research, 4(1), 151-160. Refernas28 Sharma, P., & Bajaj, B. K. (2005). Production and partial characterization of alkali-tolerant xylanase from an alkalophilic Streptomyces sp. CD3. Journal of Scientific & Industrial Research. 64: 688-697. Refernas29 Shirahama, T., Furumai, T., & Okanishi, M. (1981). A modified regeneration method for streptomycete protoplasts. Agricultural and Biological Chemistry, 45(5), 1271-1273. Refernas30 Sommer, P., Bormann, C., & Götz, F. (1997). Genetic and biochemical characterization of a new extracellular lipase from Streptomyces cinnamomeus. Applied Environmental Microbiology, 63(9), 3553-3560. Refernas31 Teeradakorn, S., Talawanich, Y., Suzuki, I., Pinphanichakarn, P., Fujiyama, K., Sekiand, T., & Yoshida, T. (1998). Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces griseoruber 42-9. World Journal of Microbiology and Biotechnology, 14(4), 565-570. Refernas32 Techapun, C., Poosaran, N., Watanabe, M., and Sasaki, K. (2003). Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review. Process Biochemistry, 38(9), 1327-1340. Refernas33 Trigo, C., and Ball, A. S. (1994). Is the solubilized product from the degradation of lignocellulose by actinomycetes a precursor of humic substances. Microbiology, 140(11), 3145-3152. Refernas34 Wang, S. L., Yen, Y. H., Shih, L., Chang, A. C., Chang, W. T., Wu, W. C., and Chai, Y. D. (2003). Production of xylanases from rice bran by Streptomyces actuosus A-151. Enzyme and Microbial Technology, 33(7), 917-925. Refernas35 Vigal, T., Martin, J. F., and Gil, J. A. (1994). Expression of the Streptomyces griseus α-amylase gene in Escherichia coli. FEMS Microbiology Letters, 118(3), 259-263. Refernas36 Vujaklija, D., Schröder, W., Abramić, M., Zou, P., Leščić, I., Franke, P., and Pigac, J. (2002). A novel streptomycete lipase: cloning, sequencing and high-level expression of the Streptomyces rimosus GDS (L)-lipase gene. Archives of microbiology, 178(2), 124-130. Refernas37 Zhang, Q., Li, W. J., Cui, X. L., Li, M. G., Xu, L. H., and Jiang, C. L. (2003). Streptomyces yunnanensis sp. nov., a mesophile from soils in Yunnan, China. International journal of systematic and evolutionary microbiology, 53(1), 217-221.
Year 2020,
Volume: 5 Issue: 2, 224 - 230, 30.06.2020
Abstract
Çukurova Üniversitesi’nin 5 farklı bölgesinden alınan toprak örneklerinden 3 Streptomyces suşu izole edildi. S40, S47, S48 suşlarının 7 gün sonundaki ksilanaz aktivitesi sırasıyla 50,92 U/mL, 49,51 U/mL, 47,79 U/mL tespit edilmiştir. Yüksek ksilanaz aktivitesi gösteren bu suşlar arasında gerçekleştirilen protoplast füzyon çalışması sonucu farklı koloni morfolojsi gösteren 9 suş seçilmiştir. (40-48)R2 ve (47-48)R3 suşlarının ksilanaz aktivitesi ebeveyn suşlara göre sırasıyla %25,9 ve % 31,9 oranında arttığı tespit edilmiştir. Protoplast füzyon tekniğiyle enzim aktivitesinin geliştirilebileceği gösterilmiştir.
Keywords
Supporting Institution
Çukurova Üniversitesi
Project Number
FEF2009D20
Thanks
Çukurova Üniversitesi BAP birimine katkılarından dolayı teşekkürler.
References
- Refernas1. Abdel-Aziz, M. S., Talkhan, F. N., Fadel, M., AbouZied, A. A., and Abdel-Razik, A. S. (2011). Improvement of xylanase production from Streptomyces pseudogriseolus via UV mutagenesis. Australian Journal of Basic and Applied Sciences, 5(5), 1045-1050. Refernas2. Antonopoulos, V., Hernandez, M., Arias, M., Mavrakos, E., and Ball, A. (2001). The use of extracellular enzymes from Streptomyces albus ATCC 3005 for the bleaching of eucalyptus kraft pulp. Applied microbiology and biotechnology, 57(1-2), 92-97. Refernas3 Aretz, W., Koller, K. P., and Riess, G. (1989). Proteolytic enzymes from recombinant Streptomyces lividans TK24. FEMS Microbiology Letters, 65(1-2), 31-35. Refernas4 Aygan, A., 2008. Halofilik Bacillus sp. izolasyonu, amilaz selülaz ve ksilanaz enzimlerinin üretimi, karakterizasyonu ve biyoteknolojik uygulamalarda kullanılabilirliği. Ç.Ü. Fen Bil. Enst.Fakültesi. Adana, Türkiye. 186s. Refernas5 Bailey, M. J., Biely, P., and Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270. Refernas6 Bajaj, B. K., Razdan, K., & Sharma, A. (2010). Thermoactive alkali-stable xylanase production from a newly isolated Streptomyces sp. SU 9. Indian Journal of Chemical Tecnology. Vol:17 pp. 375-380. Refernas7 Baltz, R. H. (1978). Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. Microbiology, 107(1), 93-102. Refernas8 Bandlish, R. K., Michael Hess, J., Epting, K. L., Vieille, C., and Kelly, R. M. (2002). Glucose‐to‐fructose conversion at high temperatures with xylose (glucose) isomerases from Streptomyces murinus and two hyperthermophilic Thermotoga species. Biotechnology and Bioengineering, 80(2), 185-194. Refernas9 Bhosale, H. J., Sukalkar, S. R., Uzma, S. M. Z., & Kadam, T. A. (2011). Production of xylanase by Streptomyces rameus grown on agricultural wastes. Biotechnol Bioinf Bioeng, 1(4), 505-512. Refernas10 Chun, J., Youn, H. D., Yim, Y. I., Lee, H., Kim, M. Y., Hah, Y. C., & Kang, S. O. (1997). Streptomyces seoulensis sp. nov. International Journal of Systematic and Evolutionary Microbiology, 47(2), 492-498. Refernas11 .Crawford, D. L., Lynch, J. M., Whipps, J. M., & Ousley, M. A. (1993). Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Applied Environmental Microbiology. 59(11), 3899-3905. Refernas12 Ding, C. H., Jiang, Z. Q., Li, X. T., Li, L. T., and Kusakabe, I. (2004). High activity xylanase production by Streptomyces olivaceoviridis E-86. World Journal of Microbiology and Biotechnology, 20(1), 7-10. Refernas13 Gold, M. H., Cheng, T. M., & Alic, M. (1983). Formation, fusion, and regeneration of protoplasts from wild-type and auxotrophic strains of the white rot basidiomycete Phanerochaete chrysosporium. Appl. Environ. Microbiol., 46(1), 260-263. Refernas14 Kaneko, T., Saito, K., Kawamura, Y., & Takahashi, S. (2001). Molecular cloning of acid-stable glucose isomerase gene from Streptomyces olivaceoviridis E-86 by a simple two-step PCR method, and its expression in Escherichia coli. Bioscience, Biotechnology, and Biochemistry, 65(5), 1054-1062. Refernas15 Kansoh, A. L., & Nagieb, Z. A. (2004). Xylanase and mannanase enzymes from Streptomyces galbus NR and their use in biobleaching of softwood kraft pulp. Antonie Van Leeuwenhoek, 85(2), 103-114. Refernas16 Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F., & Hopwood, D. A. (2000). Practical streptomyces genetics (Vol. 291). Norwich: John Innes Foundation. Norwich, UK, 613p. Refernas17 Lampel, J. S., & Strohl, W. R. (1986). Transformation and transfection of anthracycline-producing streptomycetes. Applied Environmental Microbiology, 51(1), 126-131. Refernas18 Lelong, C., Chevallet, M., Luche,S. and Rabilloud, T. (2009). Silver staining of proteins in 2DE gels. Two-Dimensional Electrophoresis protocols ( pp. 339-350). Humana Press. Refernas19 Li, W., Lanoot, B., Zhang, Y., Vancanneyt, M., Swings, J., & Liu, Z. (2002). Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. International Journal of Systematic and Evolutionary Microbiology, 52(5), 1629-1633. Refernas20 Li, X., Sun, B., Zhao, J., Lv, Y., Song, H., Li, E., & Zhu, Y. (2011). Production and improved bleaching abilities of a thermostable xylanase from a newly isolated Streptomyces chartreusis strain. African journal of Biotechnology, 10(64), 14132-14142. Refernas21 Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193, 265-275. Refernas22 Meyers, P. R., Porter, D. S., Omorogie, C., Pule, J. M., and Kwetane, T. (2003). Streptomyces speibonae sp. nov., a novel streptomycete with blue substrate mycelium isolated from South African soil. International Journal of Systematic and Evolutionary Microbiology, 53(3), 801-805. Refernas23 Nadia, H., El-Nassar, A., Amal, M. A., and Abeer, A. K. (2010). Xylanase production by Streptomyces lividans (NRC) and it’s application on waste paper. Australian Journal of Basic and Applied Sciences, 4(6), 1358-1368. Refernas24 Nascimento, R. P., Coelho, R. R. R., Marques, S., Alves, L., Gırio, F. M., Bon, E. P. S., & Amaral-Collaço, M. T. (2002). Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme and Microbial Technology, 31(4), 549-555. Refernas25 Pigac, J., & Schrempf, H. (1995). A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation. Applied Environmental Microbiology, 61(1), 352-356. Refernas26 Raykovska, V., Dolashka-Angelova, P., Paskaleva, D., Stoeva, S., Abashev, J., Kirkov, L., & Voelter, W. (2001). Isolation and characterization of a xylose-glucose isomerase from a new strain Streptomyces thermovulgaris 127, var. 7-86. Biochemistry and Cell Biology, 79(2), 195-205. Refernas27 Rifaat, H. M., Nagieb, Z. A., & Ahmed, Y. M. (2006). Production of xylanases by Streptomyces species and their bleaching effect on rice straw pulp. Applied Ecology and Environmental Research, 4(1), 151-160. Refernas28 Sharma, P., & Bajaj, B. K. (2005). Production and partial characterization of alkali-tolerant xylanase from an alkalophilic Streptomyces sp. CD3. Journal of Scientific & Industrial Research. 64: 688-697. Refernas29 Shirahama, T., Furumai, T., & Okanishi, M. (1981). A modified regeneration method for streptomycete protoplasts. Agricultural and Biological Chemistry, 45(5), 1271-1273. Refernas30 Sommer, P., Bormann, C., & Götz, F. (1997). Genetic and biochemical characterization of a new extracellular lipase from Streptomyces cinnamomeus. Applied Environmental Microbiology, 63(9), 3553-3560. Refernas31 Teeradakorn, S., Talawanich, Y., Suzuki, I., Pinphanichakarn, P., Fujiyama, K., Sekiand, T., & Yoshida, T. (1998). Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces griseoruber 42-9. World Journal of Microbiology and Biotechnology, 14(4), 565-570. Refernas32 Techapun, C., Poosaran, N., Watanabe, M., and Sasaki, K. (2003). Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review. Process Biochemistry, 38(9), 1327-1340. Refernas33 Trigo, C., and Ball, A. S. (1994). Is the solubilized product from the degradation of lignocellulose by actinomycetes a precursor of humic substances. Microbiology, 140(11), 3145-3152. Refernas34 Wang, S. L., Yen, Y. H., Shih, L., Chang, A. C., Chang, W. T., Wu, W. C., and Chai, Y. D. (2003). Production of xylanases from rice bran by Streptomyces actuosus A-151. Enzyme and Microbial Technology, 33(7), 917-925. Refernas35 Vigal, T., Martin, J. F., and Gil, J. A. (1994). Expression of the Streptomyces griseus α-amylase gene in Escherichia coli. FEMS Microbiology Letters, 118(3), 259-263. Refernas36 Vujaklija, D., Schröder, W., Abramić, M., Zou, P., Leščić, I., Franke, P., and Pigac, J. (2002). A novel streptomycete lipase: cloning, sequencing and high-level expression of the Streptomyces rimosus GDS (L)-lipase gene. Archives of microbiology, 178(2), 124-130. Refernas37 Zhang, Q., Li, W. J., Cui, X. L., Li, M. G., Xu, L. H., and Jiang, C. L. (2003). Streptomyces yunnanensis sp. nov., a mesophile from soils in Yunnan, China. International journal of systematic and evolutionary microbiology, 53(1), 217-221.
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Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Project Number | FEF2009D20 |
| Publication Date | June 30, 2020 |
| Submission Date | January 24, 2020 |
| Acceptance Date | June 12, 2020 |
| Published in Issue | Year 2020 Volume: 5 Issue: 2 |
Cited By
JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAB