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Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae)

Year 2023, Volume: 13 Issue: 3, 1613 - 1624, 01.09.2023

İrmak Polat Damla Amutkan Mutlu Zekiye Suludere

https://doi.org/10.21597/jist.1251065

Abstract

There are many morphological and histological studies in the world to investigate the structure of the external and internal organs of insects. These studies not only serve to illuminate the biology of insect species, but also to reveal taxonomic relationships. For this purpose, the detailed structure of the testis and accessory glands that are the main organs of the male reproductive system of Bolua turkiyae Ünal, 1999 (Orthoptera: Tettigoniidae) was searched with a light microscope, scanning electron microscope, and transmission electron microscope techniques in 2019-2020 in Gazi University, Prof. Dr. Zekiye Suludere Electron Microscope Center in this study. B. turkiyae is an endemic species distributed around Bolu province in Turkey. The reproductive system of male B. turkiyae is comprised of testis, vas deferens, and accessory glands. When the cross-sections of the oval and yellow testis were examined, it was observed that they contain many follicles. There are cysts in these follicles that have the developmental stages of the sperm. From the distal ends of the follicles to the proximal ends, the germarium, growth zone, maturation zone, and transformation zone are located respectively. In these stages, spermatogonium, spermatocyte, spermatids, and finally spermatozoa are formed. Accessory glands are hollow tubular structures of two different sizes and diameters. Single-layered cubic or pyramidal cells surround the lumen in both groups of accessory glands. It is hoped that these new data will contribute to our understanding of the structure of the male reproductive system of B. turkiyae.

Keywords

Endemic species Insect Electron microscopy Morphology Reproductive system

Supporting Institution

Çankırı Karatekin Üniversitesi BAP

Project Number

FF080120B02

Thanks

We express our thanks to Çankırı Karatekin University, BAP for supporting our study with a project number FF080120B02

References

  • Araújo, V. A., Munhoz, I. L. A. & Serrão, J. E. (2021). Morphology of the male reproductive tract in the water scavenger beetle Tropisternus collaris Fabricius, 1775 (Coleoptera: Hydrophilidae). Revista Brasileira de Entomologia, 65 (2), e20210012.
  • Aslan, D. (2018). Gryllus bimaculatus (Orthoptera: Gryllidae)'un son nimfal ve ergin evrelerdeki bazı doğal immune system aktivitelerinin karşılaştırılması. (Unpublished MSc Thesis) Ordu University, Institute of Science, Ordu.
  • Baccetti, B. (1987). Evolutionary Biology of Orthopteroid Insects. New York: John Wiley & Sons.
  • Bishop, J. L. & Lawson, F. A. (1965). Correlation of Testis Development with External Growth in the Madeira Cockroach (Orthoptera: Blattidae). Journal of the Kansas Entomological Society, 38 (3), 248-253.
  • Braswell, W. E., Andres, J. A., Maroja, L. S., Harrison, R. G., Howard, D. J. & Swanson, W. J. (2006). Identification and comparative analysis of accessory gland proteins in Orthoptera. Genome, 49 (9), 1069-1080.
  • Carlson, J. C. & Handel, M. A. (1988). Intercellular bridges and factors determining their patterns in the grasshopper testis. Journal of Morphology, 196 (2), 173-185.
  • Colonello, N. A. & Hartfelder, K. (2005). She’s my girl- male accessory gland products and their function in the reproductive biology of social bees. Apidologie, 36 (2), 231-244.
  • Dallai, R., Gottardo, M., Mercati, D., Machida, R., Mashimo, Y., Matsumura, Y., Rafael, J. A. & Beutel, R. G. (2014). Comparative morphology of spermatozoa and reproductive systems of zorapteran species from different world regions (Insecta, Zoraptera). Arthropod Structure and Development, 43 (4), 371-393.
  • Demir, E. (2015). Tekirdağ'ın Tettigoniinae (Orthoptera: Tettigonidae) Faunasının Morfolojik ve Davranışsal Yönden Değerlendirilmesi. (Unpublished MSc Thesis) Namık Kemal University, Institute of Science, Tekirdağ.
  • Dumser, J. B. (1980). The regulation of spermatogenesis in insects. Annual Review of Entomology, 25 (1), 341-369.
  • Glauert, A. M. & Glauert, R. H. (1958). Araldite as an embedding medium for electron microscopy. The Journal of Cell Biology, 4(2), 191-194.
  • Gomes, L. F., Badke, J. P., Zama, U., Dolder, H. & Lino-Neto, J. (2012). Morphology of the male reproductive system and spermatozoa in Centris Fabricius, 1804 (Hymenoptera: Apidae, Centridini). Micron, 43 (6), 695-704.
  • Gullan, P. J. & Cranston, P. S. (2014). The Insects: An Outline of Entomology. Oxford: Wiley-Blackwell.
  • Happ, G. M. (1992). Maturation of the male reproductive system and its endocrine regulation. Annual Review of Entomology, 37 (1), 303-320.
  • Jamienson, B. G. M., Dallai, R. & Afzelius, B. A. (1999). Insects: Their Spermatozoa and Phylogeny. Enfield: Scientific Publishers.
  • Jones, N., Taub-Montemayor, T. & Rankin, M. A. (2013). Fluorescein-dextran sequestration in the reproductive tract of the migratory grasshopper Melanoplus sanguinipes (Orthoptera: Acrididae). Micron, 46, 80-84.
  • Kaulenas, M. S. (1976). Regional specialization for export protein synthesis in the male cricket accessory gland. Journal of Experimental Zoology, 195 (1), 81-96.
  • King, M., H. Eubel, A. H. Millar & B. Baer, 2011. Proteins within the seminal fluid are crucial to keep sperm viable in the honeybee Apis mellifera. Journal of Insect Physiology, 57 (3): 409-414.
  • Kotzé, R. C. M., N. Muller, L. Du Plessis & G. Van der Horst, 2019. The importance of insect sperm: Sperm ultrastructure of Hermetia illucens (black soldier fly). Tissue and Cell, 59: 44-50.
  • Liu, N. Y., Wu, G. X., Ze, S. Z., Yang, B. & Zhu, J. Y. (2017). Morphology and ultrastructure of the male reproductive system of the jewel wasp, Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). Journal of Asia Pacific Entomology, 20 (2), 577-582.
  • Liu, Z., Zhang, J., Ma, E. & Guo, Y. (2005). Studies on the phylogenetic relationship of Acridoidea based on the male follicle morphology (Orthoptera: Acridoidea). Oriental Insects, 39 (1), 21-32.
  • Lyu, Q. H. & Hua, B. Z. (2017). Ultrastructure of male accessory glands in the scorpionfly Sinopanorpa tincta (Navás, 1931) (Mecoptera: Panorpidae). Arthropod Structure and Development, 46 (4), 627-634.
  • Lyu, Q. H., B. B. Zhang & B. Z. Hua, 2018. Ultrastructure and function of the seminal vesicle of Bittacidae (Insecta: Mecoptera). Arthropod Structure and Development, 47 (2): 173-179.
  • Marchini, D., Del Bene, G., Viscuso, R. & Dallai, R. (2012). Sperm storage by spermatodoses in the spermatheca of Trioza alacris (Flor, 1861) Hemiptera: Psylloidea, Triozidae: a structural and ultrastructural study. Journal of Morphology, 273 (2), 195-210.
  • Marchini, D., Brundo, M. V., Sottile, L. & Viscuso, R. (2009). Structure of male accessory glands of Bolivarius siculus (Fischer) (Orthoptera: Tettigoniidae) and protein analysis of their secretions. Journal of Morphology, 270 (7), 880-891.
  • Mari, I. P., Gigliolli, A. A. S., Nanya, S. & De Brito Portela-Castro, A. L. (2018). Histological and electron microscopy observations on the testis and spermatogenesis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanillae (L., 1758) (Lepidoptera: Nymphalidae). Micron, 109, 11-21.
  • Muse, W. & Balogun, R. (1991). Morphology and histology of the accessory glands of male variegated grasshopper Zonocerus panegatus L. (Orthoptera: Pyrgomorphidae) at different ages of the adult. Nigerian Journal of Entomology, 12, 35-45.
  • Muzzi, M., Di Giulio, A., Mancini, E., Fratini, E., Cervelli, M., Gasperi, T., Mariottini, P., Persichini, T. & Bologna, M. A. (2020). The male reproductive accessory glands of the blister beetle Meloe proscarabaeus L., 1758 (Coleoptera: Meloidae): Anatomy and ultrastructure of the cantharidin-storing organs. Arthropod Structure and Development, 59, 100980.
  • Nandchahal, N. (1972). Reproductive organs of Gryllodes sigillatus (Walker) (Orthoptera: Gryllidae). Journal of Natural History, 6(2), 125-131.
  • Nelsen, O. E. (1931). Life cycle, sex differentiation, and testis development in Melanoplus differentialis (Acrididae, Orthoptera). Journal of Morphology, 51(2), 467-525.
  • Polat, I. (2016). Poecilimon cervus Karabag, 1950'un sindirim, boşaltım, dişi ve erkek üreme sisteminin ültrastrüktürel özellikleri. (Unpublished PhD Thesis) Gazi University, Institute of Science, Ankara.
  • Polat, I., Amutkan Mutlu, D. & Suludere, Z. (2020). Accessory glands of male reproductive system in Pseudochorthippus parallelus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae): A light and electron microscopic study. Microscopy Research and Technique, 83 (3), 232-238.
  • Polat, I., Amutkan Mutlu, D., Ünal, M. & Suludere, Z. (2019). Histology and ultrastructure of the testis and vas deferens in Pseudochorthippus parallelus parallelus (Orthoptera: Acrididae). Microscopy Research and Technique, 82 (9), 1461-1470.
  • Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. The Journal of cell biology, 17(1), 208.
  • Silva, D. S. M., Cossolin, J. F. S., Pereira, M. R., Lino-Neto, J., Sperber, C. F. & Serrao, J. E. (2018). Male reproductive tract and spermatozoa ultrastructure in the grasshopper Orphulella punctata (De Geer, 1773) (Insecta, Orthoptera: Caelifera). Microscopy Research and Technique, 81 (2), 250-255.
  • Sottile, L., Brundo, M. V. & Viscuso, R. (2010). Formation and rearrangement of spermatodesms in males of some Orthoptera Tettigoniidae. Tissue and Cell, 42 (1), 19-23.
  • Ünal, M. (1999). Notes on Orthoptera of Western Turkey, with description of a new genus and four new species. Journal of Orthoptera Research, 8, 243-255.
  • Ünal, M. (2019). Check-list of the Turkish Orthoptera. Web page: http://www.orthoptera-tr.org/index.php/lnks. (Date accessed: 16 January 2020).
  • Viscuso, R., Narcisi, L., Sottile, L. & Brundo, M. V. (2001). Role of male accessory glands in spermatodesm reorgnization in Orthoptera: Tettigoniidea. Tissue and Cell, 33 (1), 33-39.
  • Viscuso, R., Sottile, L., Brundo, M. V. & Vitale, D. G. M. (2012). Genesis of spermatodesms in Tylopsis liliifolia (Orthoptera: Phaneropterinae) and their transit in the male genital tract. Tissue and Cell, 44 (3), 195-203.
  • Viscuso, R., Brundo, M. V., Marletta, A. & Vitale, D. G. M. (2015). Fine structure of male genital tracts of some Acrididae and Tettigoniidae (Insect: Orthoptera). Acta Zoologica, 96 (4), 418-427.
  • Vitale, D. G. M., M. V. Brundo & Viscuso, R. (2011). Morphological and ultrastructural organization of the male genital apparatus of some Aphididae (Insecta, Homoptera). Tissue and Cell, 43 (5), 271-282.
  • Vitale, D. G. M., Viscuso, R., D’Urso, V., Gibilras, S., Sardella, A., Marletta, A. & Pappalardo, A. A. (2015). Morphostructural analysis of the male reproductive system and DNA barcoding in Balclutha brevis Lindberg, 1954 (Homoptera: Cicadellidae). Micron, 79, 36-45.

Bolua turkiyae (Orthoptera, Tettigoniidae)’nin Testis ve Yardımcı Bezlerinin Morfoloji, Histoloji ve Sitolojisi

Year 2023, Volume: 13 Issue: 3, 1613 - 1624, 01.09.2023

İrmak Polat Damla Amutkan Mutlu Zekiye Suludere

https://doi.org/10.21597/jist.1251065

Abstract

Böceklerin dış ve iç organlarının yapısını araştırmak için dünyada birçok morfolojik ve histolojik çalışma bulunmaktadır. Bu çalışmalar sadece böcek türlerinin biyolojisini aydınlatmaya değil, aynı zamanda taksonomik ilişkileri ortaya çıkarmaya da hizmet etmektedir. Bu amaçla, bu çalışmada Bolua turkiyae Ünal, 1999 (Orthoptera: Tettigoniidae)'nin erkek üreme sisteminin ana organları olan testis ve yardımcı bezlerin detaylı yapısı ışık mikroskobu, taramalı elektron mikroskobu ve transmisyon elektron mikroskobu teknikleri ile 2019-2020 yıllarında Gazi Üniversitesi, Prof. Dr. Zekiye Suludere Elektron Mikroskop Merkezinde araştırılmıştır. B. turkiyae Türkiye'de Bolu İli çevresinde yayılış gösteren endemik bir türdür. Erkek B. turkiyae üreme sistemi testis, vas deferens ve yardımcı bezlerden oluşmaktadır. Oval ve sarı testislerin kesitleri incelendiğinde çok sayıda folikül içerdiği gözlenmiştir. Bu foliküllerde spermin gelişim aşamalarını içeren kistlere sahiptir. Foliküllerin distal uçlarından proksimal uçlara kadar sırasıyla germarium, büyüme bölgesi, olgunlaşma bölgesi ve transformasyon bölgesi bulunur. Bu aşamalarda spermatogonium, spermatosit, spermatidler ve son olarak spermatozoa oluşur. Yardımcı bezler, iki farklı boyut ve çapta içi boş boru şeklindeki yapılardır. Her iki yardımcı bez grubunda da tek katmanlı kübik veya piramidal hücreler lümeni çevreler. Bu yeni verilerin, B. turkiyae'nin erkek üreme sisteminin yapısı hakkındaki bilgilerimize katkıda bulunacağı umulmaktadır.

Keywords

Endemik tür Böcek Elektron mikroskopi Morfoloji Üreme sistemi

Project Number

FF080120B02

References

  • Araújo, V. A., Munhoz, I. L. A. & Serrão, J. E. (2021). Morphology of the male reproductive tract in the water scavenger beetle Tropisternus collaris Fabricius, 1775 (Coleoptera: Hydrophilidae). Revista Brasileira de Entomologia, 65 (2), e20210012.
  • Aslan, D. (2018). Gryllus bimaculatus (Orthoptera: Gryllidae)'un son nimfal ve ergin evrelerdeki bazı doğal immune system aktivitelerinin karşılaştırılması. (Unpublished MSc Thesis) Ordu University, Institute of Science, Ordu.
  • Baccetti, B. (1987). Evolutionary Biology of Orthopteroid Insects. New York: John Wiley & Sons.
  • Bishop, J. L. & Lawson, F. A. (1965). Correlation of Testis Development with External Growth in the Madeira Cockroach (Orthoptera: Blattidae). Journal of the Kansas Entomological Society, 38 (3), 248-253.
  • Braswell, W. E., Andres, J. A., Maroja, L. S., Harrison, R. G., Howard, D. J. & Swanson, W. J. (2006). Identification and comparative analysis of accessory gland proteins in Orthoptera. Genome, 49 (9), 1069-1080.
  • Carlson, J. C. & Handel, M. A. (1988). Intercellular bridges and factors determining their patterns in the grasshopper testis. Journal of Morphology, 196 (2), 173-185.
  • Colonello, N. A. & Hartfelder, K. (2005). She’s my girl- male accessory gland products and their function in the reproductive biology of social bees. Apidologie, 36 (2), 231-244.
  • Dallai, R., Gottardo, M., Mercati, D., Machida, R., Mashimo, Y., Matsumura, Y., Rafael, J. A. & Beutel, R. G. (2014). Comparative morphology of spermatozoa and reproductive systems of zorapteran species from different world regions (Insecta, Zoraptera). Arthropod Structure and Development, 43 (4), 371-393.
  • Demir, E. (2015). Tekirdağ'ın Tettigoniinae (Orthoptera: Tettigonidae) Faunasının Morfolojik ve Davranışsal Yönden Değerlendirilmesi. (Unpublished MSc Thesis) Namık Kemal University, Institute of Science, Tekirdağ.
  • Dumser, J. B. (1980). The regulation of spermatogenesis in insects. Annual Review of Entomology, 25 (1), 341-369.
  • Glauert, A. M. & Glauert, R. H. (1958). Araldite as an embedding medium for electron microscopy. The Journal of Cell Biology, 4(2), 191-194.
  • Gomes, L. F., Badke, J. P., Zama, U., Dolder, H. & Lino-Neto, J. (2012). Morphology of the male reproductive system and spermatozoa in Centris Fabricius, 1804 (Hymenoptera: Apidae, Centridini). Micron, 43 (6), 695-704.
  • Gullan, P. J. & Cranston, P. S. (2014). The Insects: An Outline of Entomology. Oxford: Wiley-Blackwell.
  • Happ, G. M. (1992). Maturation of the male reproductive system and its endocrine regulation. Annual Review of Entomology, 37 (1), 303-320.
  • Jamienson, B. G. M., Dallai, R. & Afzelius, B. A. (1999). Insects: Their Spermatozoa and Phylogeny. Enfield: Scientific Publishers.
  • Jones, N., Taub-Montemayor, T. & Rankin, M. A. (2013). Fluorescein-dextran sequestration in the reproductive tract of the migratory grasshopper Melanoplus sanguinipes (Orthoptera: Acrididae). Micron, 46, 80-84.
  • Kaulenas, M. S. (1976). Regional specialization for export protein synthesis in the male cricket accessory gland. Journal of Experimental Zoology, 195 (1), 81-96.
  • King, M., H. Eubel, A. H. Millar & B. Baer, 2011. Proteins within the seminal fluid are crucial to keep sperm viable in the honeybee Apis mellifera. Journal of Insect Physiology, 57 (3): 409-414.
  • Kotzé, R. C. M., N. Muller, L. Du Plessis & G. Van der Horst, 2019. The importance of insect sperm: Sperm ultrastructure of Hermetia illucens (black soldier fly). Tissue and Cell, 59: 44-50.
  • Liu, N. Y., Wu, G. X., Ze, S. Z., Yang, B. & Zhu, J. Y. (2017). Morphology and ultrastructure of the male reproductive system of the jewel wasp, Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). Journal of Asia Pacific Entomology, 20 (2), 577-582.
  • Liu, Z., Zhang, J., Ma, E. & Guo, Y. (2005). Studies on the phylogenetic relationship of Acridoidea based on the male follicle morphology (Orthoptera: Acridoidea). Oriental Insects, 39 (1), 21-32.
  • Lyu, Q. H. & Hua, B. Z. (2017). Ultrastructure of male accessory glands in the scorpionfly Sinopanorpa tincta (Navás, 1931) (Mecoptera: Panorpidae). Arthropod Structure and Development, 46 (4), 627-634.
  • Lyu, Q. H., B. B. Zhang & B. Z. Hua, 2018. Ultrastructure and function of the seminal vesicle of Bittacidae (Insecta: Mecoptera). Arthropod Structure and Development, 47 (2): 173-179.
  • Marchini, D., Del Bene, G., Viscuso, R. & Dallai, R. (2012). Sperm storage by spermatodoses in the spermatheca of Trioza alacris (Flor, 1861) Hemiptera: Psylloidea, Triozidae: a structural and ultrastructural study. Journal of Morphology, 273 (2), 195-210.
  • Marchini, D., Brundo, M. V., Sottile, L. & Viscuso, R. (2009). Structure of male accessory glands of Bolivarius siculus (Fischer) (Orthoptera: Tettigoniidae) and protein analysis of their secretions. Journal of Morphology, 270 (7), 880-891.
  • Mari, I. P., Gigliolli, A. A. S., Nanya, S. & De Brito Portela-Castro, A. L. (2018). Histological and electron microscopy observations on the testis and spermatogenesis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanillae (L., 1758) (Lepidoptera: Nymphalidae). Micron, 109, 11-21.
  • Muse, W. & Balogun, R. (1991). Morphology and histology of the accessory glands of male variegated grasshopper Zonocerus panegatus L. (Orthoptera: Pyrgomorphidae) at different ages of the adult. Nigerian Journal of Entomology, 12, 35-45.
  • Muzzi, M., Di Giulio, A., Mancini, E., Fratini, E., Cervelli, M., Gasperi, T., Mariottini, P., Persichini, T. & Bologna, M. A. (2020). The male reproductive accessory glands of the blister beetle Meloe proscarabaeus L., 1758 (Coleoptera: Meloidae): Anatomy and ultrastructure of the cantharidin-storing organs. Arthropod Structure and Development, 59, 100980.
  • Nandchahal, N. (1972). Reproductive organs of Gryllodes sigillatus (Walker) (Orthoptera: Gryllidae). Journal of Natural History, 6(2), 125-131.
  • Nelsen, O. E. (1931). Life cycle, sex differentiation, and testis development in Melanoplus differentialis (Acrididae, Orthoptera). Journal of Morphology, 51(2), 467-525.
  • Polat, I. (2016). Poecilimon cervus Karabag, 1950'un sindirim, boşaltım, dişi ve erkek üreme sisteminin ültrastrüktürel özellikleri. (Unpublished PhD Thesis) Gazi University, Institute of Science, Ankara.
  • Polat, I., Amutkan Mutlu, D. & Suludere, Z. (2020). Accessory glands of male reproductive system in Pseudochorthippus parallelus parallelus (Zetterstedt, 1821) (Orthoptera: Acrididae): A light and electron microscopic study. Microscopy Research and Technique, 83 (3), 232-238.
  • Polat, I., Amutkan Mutlu, D., Ünal, M. & Suludere, Z. (2019). Histology and ultrastructure of the testis and vas deferens in Pseudochorthippus parallelus parallelus (Orthoptera: Acrididae). Microscopy Research and Technique, 82 (9), 1461-1470.
  • Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. The Journal of cell biology, 17(1), 208.
  • Silva, D. S. M., Cossolin, J. F. S., Pereira, M. R., Lino-Neto, J., Sperber, C. F. & Serrao, J. E. (2018). Male reproductive tract and spermatozoa ultrastructure in the grasshopper Orphulella punctata (De Geer, 1773) (Insecta, Orthoptera: Caelifera). Microscopy Research and Technique, 81 (2), 250-255.
  • Sottile, L., Brundo, M. V. & Viscuso, R. (2010). Formation and rearrangement of spermatodesms in males of some Orthoptera Tettigoniidae. Tissue and Cell, 42 (1), 19-23.
  • Ünal, M. (1999). Notes on Orthoptera of Western Turkey, with description of a new genus and four new species. Journal of Orthoptera Research, 8, 243-255.
  • Ünal, M. (2019). Check-list of the Turkish Orthoptera. Web page: http://www.orthoptera-tr.org/index.php/lnks. (Date accessed: 16 January 2020).
  • Viscuso, R., Narcisi, L., Sottile, L. & Brundo, M. V. (2001). Role of male accessory glands in spermatodesm reorgnization in Orthoptera: Tettigoniidea. Tissue and Cell, 33 (1), 33-39.
  • Viscuso, R., Sottile, L., Brundo, M. V. & Vitale, D. G. M. (2012). Genesis of spermatodesms in Tylopsis liliifolia (Orthoptera: Phaneropterinae) and their transit in the male genital tract. Tissue and Cell, 44 (3), 195-203.
  • Viscuso, R., Brundo, M. V., Marletta, A. & Vitale, D. G. M. (2015). Fine structure of male genital tracts of some Acrididae and Tettigoniidae (Insect: Orthoptera). Acta Zoologica, 96 (4), 418-427.
  • Vitale, D. G. M., M. V. Brundo & Viscuso, R. (2011). Morphological and ultrastructural organization of the male genital apparatus of some Aphididae (Insecta, Homoptera). Tissue and Cell, 43 (5), 271-282.
  • Vitale, D. G. M., Viscuso, R., D’Urso, V., Gibilras, S., Sardella, A., Marletta, A. & Pappalardo, A. A. (2015). Morphostructural analysis of the male reproductive system and DNA barcoding in Balclutha brevis Lindberg, 1954 (Homoptera: Cicadellidae). Micron, 79, 36-45.
There are 43 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Biyoloji / Biology
Authors

İrmak Polat 0000-0001-7230-4589

Damla Amutkan Mutlu 0000-0002-4780-8520

Zekiye Suludere 0000-0002-1207-5814

Project Number FF080120B02
Early Pub Date August 29, 2023
Publication Date September 1, 2023
Submission Date February 15, 2023
Acceptance Date April 28, 2023
Published in Issue Year 2023 Volume: 13 Issue: 3

Cite

APA Polat, İ., Amutkan Mutlu, D., & Suludere, Z. (2023). Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae). Journal of the Institute of Science and Technology, 13(3), 1613-1624. https://doi.org/10.21597/jist.1251065
AMA Polat İ, Amutkan Mutlu D, Suludere Z. Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae). J. Inst. Sci. and Tech. September 2023;13(3):1613-1624. doi:10.21597/jist.1251065
Chicago Polat, İrmak, Damla Amutkan Mutlu, and Zekiye Suludere. “Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua Turkiyae (Orthoptera, Tettigoniidae)”. Journal of the Institute of Science and Technology 13, no. 3 (September 2023): 1613-24. https://doi.org/10.21597/jist.1251065.
EndNote Polat İ, Amutkan Mutlu D, Suludere Z (September 1, 2023) Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae). Journal of the Institute of Science and Technology 13 3 1613–1624.
IEEE İ. Polat, D. Amutkan Mutlu, and Z. Suludere, “Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae)”, J. Inst. Sci. and Tech., vol. 13, no. 3, pp. 1613–1624, 2023, doi: 10.21597/jist.1251065.
ISNAD Polat, İrmak et al. “Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua Turkiyae (Orthoptera, Tettigoniidae)”. Journal of the Institute of Science and Technology 13/3 (September 2023), 1613-1624. https://doi.org/10.21597/jist.1251065.
JAMA Polat İ, Amutkan Mutlu D, Suludere Z. Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae). J. Inst. Sci. and Tech. 2023;13:1613–1624.
MLA Polat, İrmak et al. “Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua Turkiyae (Orthoptera, Tettigoniidae)”. Journal of the Institute of Science and Technology, vol. 13, no. 3, 2023, pp. 1613-24, doi:10.21597/jist.1251065.
Vancouver Polat İ, Amutkan Mutlu D, Suludere Z. Morphology, Histology, and Cytology of the Testis and Accessory Glands of Bolua turkiyae (Orthoptera, Tettigoniidae). J. Inst. Sci. and Tech. 2023;13(3):1613-24.
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