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Determination of Antioxidant and Antimicrobial Activities, Phenolic Composition and Volatile Compounds of Methanol Extracts of Erodium cicutarium (L.) L'Hér. Consumed As a Vegetable in Kilis

Year 2023, Volume: 13 Issue: 4, 1460 - 1475, 15.12.2023
https://doi.org/10.31466/kfbd.1298517

Abstract

The aim of this study is to investigate the antioxidant and antibacterial activities, phenolic profile and volatile compounds of methanol extract of Erodium cicutarium (L.) L'Hér, which is consumed as a vegetable in Kilis. The total phenolic substance content, total flavonoid substance amount, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical removal and ascorbic acid content values of the extract are respectively; 1.93 mg GAE/g extract, 0.066 mg RE/g extract, 73.19% and 339.87 mg/L. Antioxidant activity, metal chelating activity, reducing capacity and CUPRAC values by phosphomolybdenum method, respectively; 2.41 µg/TE g, 86.13%, 2.868 abs., 160.78 mg trolox equivalent/g sample. Total antioxidant activity was calculated as % inhibition and trolox equivalent, these values are 51.40% and 9.56 µM trolox equivalent/10g, respectively. According to the phenolic component results, the amount of naringin, the phenolic component that was detected in the highest amount, was 1143.8993 mg/kg; the lowest detected component is t-ferulic acid and its amount is 5.08139 mg/kg. Also, in the study, the two volatile compounds detected at the highest rate were phytol with 18.88% and 2-butoxy ethanol with 13.61%; followed by p-xylene, undecane, neophytadiene and phthalic acid, butyl tetradecyl ester, respectively. In the determination of antibacterial activity, while no inhibitory effect was observed in extract against E. coli ATCC 25922 and Proteus spp. at a concentration of 100 mg/mL; an inhibitory effect against Pseudomonas aeruginosa ATCC 9027 was observed, with a zone diameter of 6 mm. The results suggested that methanol extracts of E. cicutarium may be useful for food and medical applications.

Project Number

BAP 21-13292

References

  • Al-Snafi, A.E. (2017a). Therapeutic potential of Erodium cicutarium-A review. Indo Amerıcan Journal Of Pharmaceutical Sciences, 4(2), 407-413.
  • Al-Snafi, A. E. (2017b). Medicinal plants possessed antioxidant and free radical scavenging effects (part 3)-A review. IOSR Journal of Pharmacy, 7(4), 48-62.
  • Apaydın, E. (2008). Nar Suyu Konsantresi Üretim ve Depolama Sürecinde AntioksidanAkivitedeki Değişimler. Yüksek Lisans Tezi, Gıda Mühendisliği Anabilim Dalı, Fen Bilimleri Enstüsü, Ankara Üniversitesi, Ankara, Türkiye.
  • Aydin, S., Yilmaz, O., and Gokce, Z. (2015). Protective effect of Morus nigra L.(mulberry) fruit extract on the liver fatty acid profile of Wistar rats. Pakistan Journal Zoology, 47(1), 255-261.
  • Baltaci, N., Aydogdu, N., Sarikurkcu, C., and Tepe, B. (2021). Onosma gracilis (Trautv.) and O. oreodoxa (Boiss. & Heldr.): Phytochemistry, in silico docking, antioxidant and enzyme inhibitory activities. South African Journal of Botany, 143, 410-417.
  • Bauer A.W., Kirby W.M.M., Sherris J.C. and Turck M. (1966).Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 1966; 45: 493-6.
  • Baytop, T. (1999). Therapy with medicinal plants in Turkey past and present, 2nd ed. Nobel Tıp Kitabevi, Istanbul.
  • Çelikler, Ö. (2017). Bitkisel kaynaklı yeni tirozinaz inhibitörlerinin belirlenmesi üzerinde farmakognozik araştırmalar. Doktora Tezi, Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Ankara.
  • Çelikler Özer, Ö., Kekilli, E. B., Kahraman, A. ve Orhan, İ.E. (2020). Erodium L’her. (Dönbaba/İğnelik). Türk Farmakope Dergisi, 5(1): 58-80.
  • Dinis, T.C.P., Madeira, V.I.M.C. and Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen. salicylate. and 5-aminosalicylate) assay inhibitors of membrane lipid peroxidation and assay peroxyl radical scavengers. Archives of Biochemistry and Biophysics, vol.315. no.1. pp.161-169.
  • Doğan, H. (2022). Erodium cicutarium (İğnelik)-Kocaeli Bitkileri. Erişim adresi: https://kocaelibitkileri.com/erodium-cicutarium/ (Erişim tarihi: 18 April 2023).
  • Fecka, K. Gasiorowska and B. Brokos. (1997). ‘Analiza fitochemiczna i ocena immunotropowej aktywności frakcji polifenolowej z ziela iglicy pospolitej (Erodium cicutarium (L.) L’Herit.)’, Herba Polonica Journal, 43, 214–221.
  • Hısıl, Y. (2004). Enstrümental Gıda Analizleri-Laboratuvar Deneyleri. Ege Üniversitesi, Mühendislik Fakültesi, Ders Kitapları, Bornova, İzmir, Yayın No. 45, 39 pp.
  • Ljoljić Bilić, V., Stabentheiner, E., Kremer, D., Dunkić, V., Grubešić, R. J., and Rodríguez, J. V. (2019). Phytochemical and micromorphological characterization of croatian populations of Erodium cicutarium. Natural Product Communications, 14(6), 1-8. DOI: 1934578X19856257.
  • Ljoljić Bilić, V., Gašić, U.M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek-Gorski, D. and Kosalec, I. (2022). Antibacterial fractions from Erodium cicutarium exposed—clinical strains of Staphylococcus aureus in focus. Antibiotics, 11(4), 492.
  • Lis-Balchin, M. (1993). The essential oils of Pelargonium grossularioides and Erodium cicutarium (Geraniaceae). Journal of Essential Oil Research, 5, 317–318.
  • Mohiseni, M. (2017). Medicinal herbs, strong source of antioxidant in aquaculture: A mini review. Modern Applications in Pharmacy & Pharmacology, 1(1) ), pp. 1-5, 10.31031/mapp.2017.01.000504.
  • Mizzi, L., Chatzitzika, C., Gatt, R., and Valdramidis, V. (2020). HPLC analysis of phenolic compounds and flavonoids with overlapping peaks. Food technology and biotechnology, 58(1), 12-19.
  • Mradu, G., Saumyakanti, S., Sohini, M., and Arup, M. (2012). HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research, 4(3), 162-167.
  • Munekata, P. E., Alcántara, C., Collado, M. C., Garcia-Perez, J. V., Saraiva, J. A., Lopes, R. P., and Lorenzo, J. M. (2019). Ethnopharmacology, phytochemistry and biological activity of Erodium species: A review. Food research international, 126, 108659.
  • Nikitina, V. S., Kuz’mina, L. Yu., Melent’ev, A. I., and Shendel’, G. V. (2007). Antibacterial activity of polyphenolic compounds isolated from plants of Geraniaceae and Rosaceae families. Applied Biochemistry and Microbiology, 43, 629– 634. DOI: 10.1134/S0003683807060117.
  • Radulovi´c, N., Deki´c, M., Stojanovi´c-Radi´c, Z. and Pali´c, R. (2009). Volatile constituents of Erodium cicutarium (L.) L’ Hérit. (Geraniaceae). Central European Journal of Biology, 4, 404–410. DOI: 10.2478/s11535-009-0026-0.
  • Sabatini, N., Mucciarella, M.R. and Marsilio, V. (2008). Volatile compounds in uninoculated and ınoculated table olives with Lactobacillus plantarum (Olea Europaea L., cv. Moresca and Kalamata). Food Science Technology, 41, 2017-2022.
  • Sarikurkcu, C., Targan, S., Ozer, M. S., and Tepe, B. (2017). Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey. International Journal of Food Properties, 20(3), 560-572.
  • Sharm S, Vig P.A. (2013). Evaluation of In vitro Antioxidant Properties of Methanol and Aqueous Extracts of Parkinsona aculeata L. Leaves. The Scientific World Journal, 1-7. https://doi.org/10.1155/2013/604865
  • Sroka, Z., Bodalska, H. R., and Mażol, I. (1994). Antioxidative effect of extracts from Erodium cicutarium L. Zeitschrift für Naturforschung C, 49(11-12), 881-884.
  • Stanković M.S. (2011). Total Phenolic Content, Flavonoid Concentration and antioxidant Activity of Marrubium Peregrinum L. Extracts. Kragujevac Journal of Science. 33, 63-72.
  • Stojanović-Radić, Z., Čomić, L., Radulović, N., Dekić, M., Ranđelović, V., and Stefanović, O. (2010). Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd.(Geraniaceae). Chemical Papers, 64(3), 368-377.
  • Tetik, F. (2011). Malatya ilinin etnobotanik değeri olan bitkileri üzerine bir araştırma. Yüksek Lisans Tezi, Fırat Üniversitesi, Fen Bilimleri Enstitüsü, Elazığ.
  • Ucan Turkmen, F. and Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038-1046.
  • Ucan Turkmen, F., Mercimek Takci, H.A. and Sarigullu Önalan, F.E. (2020). Evaluation of antioxidant activity of sour cherry stalk extracts by in vitro methods. Karadeniz Fen Bilimleri Dergisi, 10(2), 290-301.
  • Ucan Turkmen, F., Sarigullu Onalan, F.E. and Mercimek Takci, H.A. (2021). Antioxidant activities of pomegranate peel methanolic and water extracts by in vitro methods. Natural Science and Discovery, 4(1), 1-6.
  • Zengin, G., Sarikurkcu, C., Aktumsek, A., Ceylan, R. and Ceylan, O. (2014). A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes. Industrial Crops and Products, 53, 244-251.

Kilis'te Sebze Olarak Tüketilen Erodium cicutarium (L.) L'Hér.'un Metanol Ekstraktının Antioksidan ve Antibakteriyal Aktiviteleri, Fenolik Bileşimi ile Aroma Bileşiklerinin Belirlenmesi

Year 2023, Volume: 13 Issue: 4, 1460 - 1475, 15.12.2023
https://doi.org/10.31466/kfbd.1298517

Abstract

Bu çalışmanın amacı, Kilis'te sebze olarak tüketilmekte olan Erodium cicutarium (L.) L'Hér 'ün metanol ekstraktının antioksidan ve antibakteriyal aktivitelerini, fenolik profilini ve aroma bileşimini araştırmaktır. Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı, DPPH (1,1-diphenyl-2-picryl hydrazyl) radikal giderimi ve askorbik asit miktarı değerleri sırasıyla; 1.93 mg GAE/g ekstrakt, 0.066 mg RE/g ekstrakt, % 73.19 ve 339.87 mg/L’dir. Fosfomolibdenyum yöntemi ile antioksidan aktivitesi, metal şelatlama aktivitesi, indirgeme kapasitesi ve CUPRAC değerleri sırasıyla; 2.41 µg/TE g, %86.13, 2.868 abs., 160.78 mg troloks eşdeğeri/g örnek’dir. Toplam antioksidan aktivite ise % inhibisyon ve troloks eşdeğeri olarak hesaplanmış olup, bu değerler sırasıyla %51.40 ve 9.56 µM troloks eşdeğeri/10g’dır. Fenolik bileşen sonuçlarına göre, en yüksek miktarda tespit edilmiş olan fenolik bileşen naringinin miktarı 1143.8993 mg/kg iken; en düşük tespit edilen bileşen ise t-ferulik asit olup miktarı 5.08139 mg/kg’dır. Çalışmada en yüksek oranda tespit edilen iki aroma bileşiği %18.88 ile fitol ve %13.61 ile 2-bütoksi etanol olurken; bunları sırası ile p-ksilen, undekan, neofitadien ve fitalik asit, bütil tetradesil ester takip etmiştir. Antibakteriyal aktivite tayininde, 100 mg/mL konsantrasyonda E. coli ATCC 25922 ve Proteus spp.’ye karşı özütlerde herhangi bir inhibitör etkiye rastlanmazken; Pseudomonas aeruginosa ATCC 9027’ye karşı inhibitör etki gözlenmiş ve zon çapı 6 mm olarak ölçülmüştür. Sonuçlar, E. cicutarium’un metanol ekstraktının gıda ve tıbbi uygulamalar için yararlı olabileceğini düşündürmüştür.

Supporting Institution

Kilis 7 Aralık Üniversitesi Bilimsel Araştırma ve Projeler Birimi

Project Number

BAP 21-13292

Thanks

Bu çalışma Kilis 7 Aralık Üniversitesi Bilimsel Araştırma ve Projeler Birimi tarafından desteklendiği için, birime desteklerinden dolayı teşekkür ederiz (Proje No: BAP 21-13292).

References

  • Al-Snafi, A.E. (2017a). Therapeutic potential of Erodium cicutarium-A review. Indo Amerıcan Journal Of Pharmaceutical Sciences, 4(2), 407-413.
  • Al-Snafi, A. E. (2017b). Medicinal plants possessed antioxidant and free radical scavenging effects (part 3)-A review. IOSR Journal of Pharmacy, 7(4), 48-62.
  • Apaydın, E. (2008). Nar Suyu Konsantresi Üretim ve Depolama Sürecinde AntioksidanAkivitedeki Değişimler. Yüksek Lisans Tezi, Gıda Mühendisliği Anabilim Dalı, Fen Bilimleri Enstüsü, Ankara Üniversitesi, Ankara, Türkiye.
  • Aydin, S., Yilmaz, O., and Gokce, Z. (2015). Protective effect of Morus nigra L.(mulberry) fruit extract on the liver fatty acid profile of Wistar rats. Pakistan Journal Zoology, 47(1), 255-261.
  • Baltaci, N., Aydogdu, N., Sarikurkcu, C., and Tepe, B. (2021). Onosma gracilis (Trautv.) and O. oreodoxa (Boiss. & Heldr.): Phytochemistry, in silico docking, antioxidant and enzyme inhibitory activities. South African Journal of Botany, 143, 410-417.
  • Bauer A.W., Kirby W.M.M., Sherris J.C. and Turck M. (1966).Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 1966; 45: 493-6.
  • Baytop, T. (1999). Therapy with medicinal plants in Turkey past and present, 2nd ed. Nobel Tıp Kitabevi, Istanbul.
  • Çelikler, Ö. (2017). Bitkisel kaynaklı yeni tirozinaz inhibitörlerinin belirlenmesi üzerinde farmakognozik araştırmalar. Doktora Tezi, Gazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Ankara.
  • Çelikler Özer, Ö., Kekilli, E. B., Kahraman, A. ve Orhan, İ.E. (2020). Erodium L’her. (Dönbaba/İğnelik). Türk Farmakope Dergisi, 5(1): 58-80.
  • Dinis, T.C.P., Madeira, V.I.M.C. and Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen. salicylate. and 5-aminosalicylate) assay inhibitors of membrane lipid peroxidation and assay peroxyl radical scavengers. Archives of Biochemistry and Biophysics, vol.315. no.1. pp.161-169.
  • Doğan, H. (2022). Erodium cicutarium (İğnelik)-Kocaeli Bitkileri. Erişim adresi: https://kocaelibitkileri.com/erodium-cicutarium/ (Erişim tarihi: 18 April 2023).
  • Fecka, K. Gasiorowska and B. Brokos. (1997). ‘Analiza fitochemiczna i ocena immunotropowej aktywności frakcji polifenolowej z ziela iglicy pospolitej (Erodium cicutarium (L.) L’Herit.)’, Herba Polonica Journal, 43, 214–221.
  • Hısıl, Y. (2004). Enstrümental Gıda Analizleri-Laboratuvar Deneyleri. Ege Üniversitesi, Mühendislik Fakültesi, Ders Kitapları, Bornova, İzmir, Yayın No. 45, 39 pp.
  • Ljoljić Bilić, V., Stabentheiner, E., Kremer, D., Dunkić, V., Grubešić, R. J., and Rodríguez, J. V. (2019). Phytochemical and micromorphological characterization of croatian populations of Erodium cicutarium. Natural Product Communications, 14(6), 1-8. DOI: 1934578X19856257.
  • Ljoljić Bilić, V., Gašić, U.M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek-Gorski, D. and Kosalec, I. (2022). Antibacterial fractions from Erodium cicutarium exposed—clinical strains of Staphylococcus aureus in focus. Antibiotics, 11(4), 492.
  • Lis-Balchin, M. (1993). The essential oils of Pelargonium grossularioides and Erodium cicutarium (Geraniaceae). Journal of Essential Oil Research, 5, 317–318.
  • Mohiseni, M. (2017). Medicinal herbs, strong source of antioxidant in aquaculture: A mini review. Modern Applications in Pharmacy & Pharmacology, 1(1) ), pp. 1-5, 10.31031/mapp.2017.01.000504.
  • Mizzi, L., Chatzitzika, C., Gatt, R., and Valdramidis, V. (2020). HPLC analysis of phenolic compounds and flavonoids with overlapping peaks. Food technology and biotechnology, 58(1), 12-19.
  • Mradu, G., Saumyakanti, S., Sohini, M., and Arup, M. (2012). HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research, 4(3), 162-167.
  • Munekata, P. E., Alcántara, C., Collado, M. C., Garcia-Perez, J. V., Saraiva, J. A., Lopes, R. P., and Lorenzo, J. M. (2019). Ethnopharmacology, phytochemistry and biological activity of Erodium species: A review. Food research international, 126, 108659.
  • Nikitina, V. S., Kuz’mina, L. Yu., Melent’ev, A. I., and Shendel’, G. V. (2007). Antibacterial activity of polyphenolic compounds isolated from plants of Geraniaceae and Rosaceae families. Applied Biochemistry and Microbiology, 43, 629– 634. DOI: 10.1134/S0003683807060117.
  • Radulovi´c, N., Deki´c, M., Stojanovi´c-Radi´c, Z. and Pali´c, R. (2009). Volatile constituents of Erodium cicutarium (L.) L’ Hérit. (Geraniaceae). Central European Journal of Biology, 4, 404–410. DOI: 10.2478/s11535-009-0026-0.
  • Sabatini, N., Mucciarella, M.R. and Marsilio, V. (2008). Volatile compounds in uninoculated and ınoculated table olives with Lactobacillus plantarum (Olea Europaea L., cv. Moresca and Kalamata). Food Science Technology, 41, 2017-2022.
  • Sarikurkcu, C., Targan, S., Ozer, M. S., and Tepe, B. (2017). Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey. International Journal of Food Properties, 20(3), 560-572.
  • Sharm S, Vig P.A. (2013). Evaluation of In vitro Antioxidant Properties of Methanol and Aqueous Extracts of Parkinsona aculeata L. Leaves. The Scientific World Journal, 1-7. https://doi.org/10.1155/2013/604865
  • Sroka, Z., Bodalska, H. R., and Mażol, I. (1994). Antioxidative effect of extracts from Erodium cicutarium L. Zeitschrift für Naturforschung C, 49(11-12), 881-884.
  • Stanković M.S. (2011). Total Phenolic Content, Flavonoid Concentration and antioxidant Activity of Marrubium Peregrinum L. Extracts. Kragujevac Journal of Science. 33, 63-72.
  • Stojanović-Radić, Z., Čomić, L., Radulović, N., Dekić, M., Ranđelović, V., and Stefanović, O. (2010). Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd.(Geraniaceae). Chemical Papers, 64(3), 368-377.
  • Tetik, F. (2011). Malatya ilinin etnobotanik değeri olan bitkileri üzerine bir araştırma. Yüksek Lisans Tezi, Fırat Üniversitesi, Fen Bilimleri Enstitüsü, Elazığ.
  • Ucan Turkmen, F. and Mercimek Takci, H.A. (2018). Ultraviolet-C and ultraviolet-B lights effect on black carrot (Daucus carota ssp. sativus) juice. Journal of Food Measurement and Characterization, 12, 1038-1046.
  • Ucan Turkmen, F., Mercimek Takci, H.A. and Sarigullu Önalan, F.E. (2020). Evaluation of antioxidant activity of sour cherry stalk extracts by in vitro methods. Karadeniz Fen Bilimleri Dergisi, 10(2), 290-301.
  • Ucan Turkmen, F., Sarigullu Onalan, F.E. and Mercimek Takci, H.A. (2021). Antioxidant activities of pomegranate peel methanolic and water extracts by in vitro methods. Natural Science and Discovery, 4(1), 1-6.
  • Zengin, G., Sarikurkcu, C., Aktumsek, A., Ceylan, R. and Ceylan, O. (2014). A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes. Industrial Crops and Products, 53, 244-251.
There are 33 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Filiz Uçan Türkmen 0000-0002-3653-9433

Gülcan Koyuncu 0000-0001-7406-5331

Fatma Esen Sarıgüllü Önalan 0000-0002-1374-4338

Ümit Haydar Erol 0000-0001-6126-5844

Project Number BAP 21-13292
Early Pub Date December 18, 2023
Publication Date December 15, 2023
Published in Issue Year 2023 Volume: 13 Issue: 4

Cite

APA Uçan Türkmen, F., Koyuncu, G., Sarıgüllü Önalan, F. E., Erol, Ü. H. (2023). Kilis’te Sebze Olarak Tüketilen Erodium cicutarium (L.) L’Hér.’un Metanol Ekstraktının Antioksidan ve Antibakteriyal Aktiviteleri, Fenolik Bileşimi ile Aroma Bileşiklerinin Belirlenmesi. Karadeniz Fen Bilimleri Dergisi, 13(4), 1460-1475. https://doi.org/10.31466/kfbd.1298517