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Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Year 2019, Cilt 22 (Ek Sayı 2), 374 - 381, 31.12.2019

Sevgi Gezici

https://doi.org/10.18016/ksutarimdoga.v22i49454.580285
Cited By: 1

Abstract

Bu çalışmada, Meşe palamudunun (Q.
coccifera) dış-kabuk, külah ve iç kısmından elde edilen
özütlerin; A549, MCF-7, HeLa kanser hücreleri ve kanserli olmayan HUVEC
hücrelerine karşı potansiyel antikanser, antiproliferatif ve nekrotik
kapasiteleri araştırılmıştır. Analizler sonucunda, meşe palamudunun farklı
kısımlarından elde edilen ekstrelerin en düşük konsantrasyonda dahi antikanser
ve antiproliferatif etkiye sahip oldukları ortaya konulmuştur. Palamut
meyvesinin külah kısmından elde edilen etanol özütleri, 5.04±0.02 ile
18.04±0.16 µg/mL arasında değişen IC50 değerleriyle, diğer
kısımlarından elde edilen özütlerden daha yüksek antikanser aktivite
göstermiştir. En yüksek oranda sitotoksik aktivite MCF-7 hücrelerine karşı
gözlenirken; en düşük sitotoksik aktivite ise A549 hücrelerinde gözlenmiştir.
Antikanser ve antiproliferatif aktivite sonuçlarının aksine, en yüksek LDH
aktivitesi HeLa hücrelerinde belirlenmiştir. MTT ve tripan mavisi analiz
sonuçlarıyla uyumlu olarak, en yüksek LDH salınım kapasitesi külah kısmından
elde etanol özütlerinde saptanmıştır. Bu çalışma; meşe palamudunun, kanser
hücrelerinde hücre büyümesini konsantrasyon ve zamana bağlı olarak engelleme
potansiyeline sahip olduğunu ortaya koyan, özgün bir çalışmadır.

Keywords

Q. coccifera L. Meşe palamudu Antikanser Antiproliferatif Sitotoksisite

Thanks

Teşekkür Bu çalışma, Kilis 7 Aralık Üniversitesi, İleri Teknoloji Araştırma ve Uygulama Merkezi ve Gaziantep Üniversitesi Tıp Fakültesi Laboratuvarlarının alt yapısı kullanılarak gerçekleştirilmiştir. Bitki materyalinin temini ve sistematik olarak tanımlanması Kilis 7 Aralık Üniversitesi Gıda Mühendisliği Bölümü Öğretim Üyesi Prof. Dr. Nazım Şekeroğlu tarafından gerçekleştirilmiştir.

References

  • Akcan T, Gokce R, Asensio M, Estevez M, Morcuende D 2017. Acorn (Quercus spp.) as a novel source of oleic acid and tocopherols for livestock and humans: discrimination of selected species from Mediterranean forest. Journal of Food Science and Technology 54, 3050–3057.
  • Akgunlu S, Sekeroglu N, Koca-Caliskan U, Ozkutlu F, Ozcelik B, Kulak M, Gezici S 2016. Research on selected wild edible vegetables: Mineral content and antimicrobial potentials. Annals of Phytomedicine 5(2): 50-57.
  • Al-Qubaisi M, Rozita R, Yeap SK, Omar AR, Ali AM 2011. Selective cytotoxicity of goniothalamin against hepatoblastoma HepG2 cells. Molecules 16(4): 2944-2959. https://doi.org/10.3390/ molecules16042944.
  • Anlas C, Bakirel TI, Ustun-Alkan F, Celik B, Yuzbasioglu Baran M, Ustuner O, Kuruuzum-Uz A, 2019. In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent. Industrial Crops and Products 137: 24-32. https://doi.org/10.1016/j.indcrop.2019.05.008.
  • Belkhodja H, Meddah B, Gezici S 2017. Anti-Inflammatory Effects of Essential Oils From Rosmarinus officinalis and Populus alba on Experimental Models of Acute and Chronic Inflammation in Rats. Indian Journal of Pharmaceutical Education and Research 51(3): 180-184. https://doi.org/10.5530ijper.51.3s.8.
  • Davis PH 1982. Flora of Turkey and the East Aegean Islands. Vol. 7, Edinburgh, UK: Edinburgh University Press.
  • Gezici S 2018. Promising anticancer activity of lavender (Lavandula angustifolia Mill.) essential oil through induction of both apoptosis and necrosis. Annals of Phytomedicine 7(2): 38-45. https://doi.org/ 10.21276/ap.2018.7.2.5.
  • Gezici S 2019. Anticancer, antiproliferative, lysosomal and lactate dehydrogenase inhibitory effects of fruit extracts from sumac (Rhus coriaria L.) on human lung cancer cells. Acta Oncologica Turcica 52(1): 160-168. https://doi.org/10.5505/aot.2019.09326.
  • Gezici S, Sekeroglu N 2019a. Neuroprotective potential and phytochemical composition of acorn fruits. Industrial Crops and Products 128: 13-17. https://doi.org/10.1016/j.indcrop.2018.10.082.
  • Gezici S, Sekeroglu N 2019b. Current perspectives in the application of medicinal plants against cancer: novel therapeutic agents. Anticancer Agents in Medicinal Chemistry 19(1): 101-111. https://doi.org/ 10.2174/1871520619666181224121004.
  • Gezici S, Sekeroglu N, Kijjoa A 2017. In vitro Anticancer Activity and Antioxidant Properties of Essential Oils from Populus alba L. and Rosmarinus officinalis L. from South Eastern Anatolia of Turkey. Indian Journal of Pharmaceutical Education and Research 51(3): 498-503. https://doi.org/10.5530/ijper.51.3s.74.
  • Gundogdu M, Tuncturk M, Berk S, Sekeroglu N, Gezici S 2018. Antioxidant Capacity and Bioactive Contents of Mulberry Species from Eastern Anatolia Region of Turkey. Indian Indian Journal of Pharmaceutical Education and Research 52(4): 96-101. https://doi.org/10.5530/ijper.52.4s.82.
  • Ito H, Yamaguchi K, Kim TH, Khennouf S, Gharzouli K, Yoshida T 2002. Dimeric and Trimeric Hydrolyzable Tannins from Quercus coccifera and Quercus suber. Journal of Natural Product 65(3): 339-345. https://doi.org/10.1021/np010465i.
  • Karik U, Cinar O, Tuncturk M, Sekeroglu N, Gezici S 2018. Essential Oil Composition of Some Sage (Salvia spp.) Species Cultivated in İzmir (Turkey) Ecological Conditions. Indian Journal of Pharmaceutical Education and Research 52(4): 102-107. https://doi.org/10.5530/ijper.52.4s.83.
  • Manoharachary C, Nagaraju D 2016. Medicinal plants for human health and welfare. Annals of Phytomedicine 5(1): 24-34.
  • Molina-García L, Martínez-Expósito R, Fernández-de Córdova ML, Llorent-Martínez EJ 2018. Determination of the Phenolic Profile and Antioxidant Activity of Leaves and Fruits of Spanish Quercus coccifera. Journal of Chemistry Article ID 2573270, 9 pages. https://doi.org/ 10.1155/2018/2573270.
  • Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65(1-2): 55-63.
  • Newman DJ, Cragg GM 2016. Natural products as sources of new drugs from 1981 to 2014. Journal of Natural Products 79(3): 629-661.
  • Ozcan T, Bayçu G, 2005. Some elemental concentrations in the acorns of Turkish Quercus L. (Fagaceae) taxa. Pakistan Journal of Botany 37 (2): 361.
  • Roleira FM, Varela CL, Costa SC, Tavares-da-Silva EJ 2018. Phenolic derivatives from medicinal herbs and plant extracts: anticancer effects and synthetic approaches to modulate biological activity. Studies in Natural Products Chemistry 57: 115-56. https ://doi.org/10.1016/B978-0-444-64057-4.00004-1.
  • Sekeroglu N, Karaoglan M, Gezici S, Kulak M, Ozkutlu F, Kacar O, Gul F 2018. Variation in the composition of the essential oils, hypericin and mineral elements in aerial parts, stem and flower of Hypericum capitatum (CHOISY) growing in Turkey with oxidative DNA damage protective activity. Journal of Pharmaceutical Research 17: 67-77. https://doi.org/10.18579/jpcrkc/2018/17/2/123613.
  • Sekeroglu N, Urlu E, Kulak M, Gezici S, Dang R 2017. Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L. Indian Journal of Pharmaceutical Education and Research 51: 1-7. https://doi.org/10.5530/ijper.51.2s.43.
  • Senol FS, Sekeroglu N, Gezici S, Kilic E, Orhan IE 2018. Neuroprotective potential of the fruit (acorn) from Quercus coccifera L. Turkish Journal of Agriculture and Forestry 42(2): 82-87. https://doi.org/10.3906/tar-1711-18.
  • Shida W, Tateishi H, Fujita M, Koga R, Radwan MO, Ciftci HI, Otsuka M, Husham AL-Saadi, D, Watanabe M, Gezici S, Wada M, Sekeroglu N, Watanabe T 2019. Anticancer activity of extract from twigs of Caucasian beech in Turkey. The Fifth International Symposium on Pharmaceutical and Biomedical Sciences (ISPBS-5), Cappadocia-Turkey, Oral presentation p: 29. www.ispbs.org.
  • Siegel RL, Miller KD, Jemal A 2018. Cancer statistics, 2018. CA: Cancer Journal for Clinicians 68(1): 7-30. https://doi.org/10.3322/caac.21442.
  • Sohretoglu D, Ekizoglu M, Kilic E, Sakar MK 2007. Antibacterial and antifungal activities of some Quercus species growing in Turkey. FABAD Journal of Pharmaceutical Sciences 32 (3):127–130.
  • Strober W 2015. Trypan blue exclusion test of cell viability. Current Protocols in Immunology 111(1): A3. B.1-A3. B.3. https://doi.org/ 10.1002/0471142735. ima03bs111.

Anticancer, Antiproliferative and Lactate Dehydrogenase Enzyme Activities of Different Parts of Acorn (Quercus coccifera L.) against A549, MCF-7 and HeLa Human Cancer Cells

Year 2019, Cilt 22 (Ek Sayı 2), 374 - 381, 31.12.2019

Sevgi Gezici

https://doi.org/10.18016/ksutarimdoga.v22i49454.580285
Cited By: 1

Abstract

In this study, potential
anticancer antiproliferative and necrotic capacities of the extracts obtained
from shell, cup and shelled acorn parts of acorn (Q. coccifera) were investigated against A549, MCF-7, HeLa cancer
cells and non-tumorous HUVECs. As a result of the analysis, it was proved that
the different parts obtained from the acorns have anticancer and
antiproliferative effects, even at the lowest concentration. The ethanol
extracts obtained from the cup parts of acorn showed higher anticancer activity
with IC50 values ranging from 5.04±0.02 to 18.04±0.16 µg/mL than the
extracts from other parts. The highest rate of cytotoxic activity was observed
against MCF-7 cells, whilst the lowest cytotoxic activity was observed against
A549 cells. In contrast to anticancer and antiproliferative activity results,
the highest LDH activity was determined in HeLa cells. In accordance with the
MTT and trypan blue assay results, the highest LDH release capacity was found
in the ethanol extracts obtained from the cup part. This is an original study
that reveals acorn has the potential to inhibit cell growth in the cancer cells
depending on concentration and time.

Keywords

Q. coccifera L. Acorn Anticancer Antiproliferative Cytotoxicity

References

  • Akcan T, Gokce R, Asensio M, Estevez M, Morcuende D 2017. Acorn (Quercus spp.) as a novel source of oleic acid and tocopherols for livestock and humans: discrimination of selected species from Mediterranean forest. Journal of Food Science and Technology 54, 3050–3057.
  • Akgunlu S, Sekeroglu N, Koca-Caliskan U, Ozkutlu F, Ozcelik B, Kulak M, Gezici S 2016. Research on selected wild edible vegetables: Mineral content and antimicrobial potentials. Annals of Phytomedicine 5(2): 50-57.
  • Al-Qubaisi M, Rozita R, Yeap SK, Omar AR, Ali AM 2011. Selective cytotoxicity of goniothalamin against hepatoblastoma HepG2 cells. Molecules 16(4): 2944-2959. https://doi.org/10.3390/ molecules16042944.
  • Anlas C, Bakirel TI, Ustun-Alkan F, Celik B, Yuzbasioglu Baran M, Ustuner O, Kuruuzum-Uz A, 2019. In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent. Industrial Crops and Products 137: 24-32. https://doi.org/10.1016/j.indcrop.2019.05.008.
  • Belkhodja H, Meddah B, Gezici S 2017. Anti-Inflammatory Effects of Essential Oils From Rosmarinus officinalis and Populus alba on Experimental Models of Acute and Chronic Inflammation in Rats. Indian Journal of Pharmaceutical Education and Research 51(3): 180-184. https://doi.org/10.5530ijper.51.3s.8.
  • Davis PH 1982. Flora of Turkey and the East Aegean Islands. Vol. 7, Edinburgh, UK: Edinburgh University Press.
  • Gezici S 2018. Promising anticancer activity of lavender (Lavandula angustifolia Mill.) essential oil through induction of both apoptosis and necrosis. Annals of Phytomedicine 7(2): 38-45. https://doi.org/ 10.21276/ap.2018.7.2.5.
  • Gezici S 2019. Anticancer, antiproliferative, lysosomal and lactate dehydrogenase inhibitory effects of fruit extracts from sumac (Rhus coriaria L.) on human lung cancer cells. Acta Oncologica Turcica 52(1): 160-168. https://doi.org/10.5505/aot.2019.09326.
  • Gezici S, Sekeroglu N 2019a. Neuroprotective potential and phytochemical composition of acorn fruits. Industrial Crops and Products 128: 13-17. https://doi.org/10.1016/j.indcrop.2018.10.082.
  • Gezici S, Sekeroglu N 2019b. Current perspectives in the application of medicinal plants against cancer: novel therapeutic agents. Anticancer Agents in Medicinal Chemistry 19(1): 101-111. https://doi.org/ 10.2174/1871520619666181224121004.
  • Gezici S, Sekeroglu N, Kijjoa A 2017. In vitro Anticancer Activity and Antioxidant Properties of Essential Oils from Populus alba L. and Rosmarinus officinalis L. from South Eastern Anatolia of Turkey. Indian Journal of Pharmaceutical Education and Research 51(3): 498-503. https://doi.org/10.5530/ijper.51.3s.74.
  • Gundogdu M, Tuncturk M, Berk S, Sekeroglu N, Gezici S 2018. Antioxidant Capacity and Bioactive Contents of Mulberry Species from Eastern Anatolia Region of Turkey. Indian Indian Journal of Pharmaceutical Education and Research 52(4): 96-101. https://doi.org/10.5530/ijper.52.4s.82.
  • Ito H, Yamaguchi K, Kim TH, Khennouf S, Gharzouli K, Yoshida T 2002. Dimeric and Trimeric Hydrolyzable Tannins from Quercus coccifera and Quercus suber. Journal of Natural Product 65(3): 339-345. https://doi.org/10.1021/np010465i.
  • Karik U, Cinar O, Tuncturk M, Sekeroglu N, Gezici S 2018. Essential Oil Composition of Some Sage (Salvia spp.) Species Cultivated in İzmir (Turkey) Ecological Conditions. Indian Journal of Pharmaceutical Education and Research 52(4): 102-107. https://doi.org/10.5530/ijper.52.4s.83.
  • Manoharachary C, Nagaraju D 2016. Medicinal plants for human health and welfare. Annals of Phytomedicine 5(1): 24-34.
  • Molina-García L, Martínez-Expósito R, Fernández-de Córdova ML, Llorent-Martínez EJ 2018. Determination of the Phenolic Profile and Antioxidant Activity of Leaves and Fruits of Spanish Quercus coccifera. Journal of Chemistry Article ID 2573270, 9 pages. https://doi.org/ 10.1155/2018/2573270.
  • Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65(1-2): 55-63.
  • Newman DJ, Cragg GM 2016. Natural products as sources of new drugs from 1981 to 2014. Journal of Natural Products 79(3): 629-661.
  • Ozcan T, Bayçu G, 2005. Some elemental concentrations in the acorns of Turkish Quercus L. (Fagaceae) taxa. Pakistan Journal of Botany 37 (2): 361.
  • Roleira FM, Varela CL, Costa SC, Tavares-da-Silva EJ 2018. Phenolic derivatives from medicinal herbs and plant extracts: anticancer effects and synthetic approaches to modulate biological activity. Studies in Natural Products Chemistry 57: 115-56. https ://doi.org/10.1016/B978-0-444-64057-4.00004-1.
  • Sekeroglu N, Karaoglan M, Gezici S, Kulak M, Ozkutlu F, Kacar O, Gul F 2018. Variation in the composition of the essential oils, hypericin and mineral elements in aerial parts, stem and flower of Hypericum capitatum (CHOISY) growing in Turkey with oxidative DNA damage protective activity. Journal of Pharmaceutical Research 17: 67-77. https://doi.org/10.18579/jpcrkc/2018/17/2/123613.
  • Sekeroglu N, Urlu E, Kulak M, Gezici S, Dang R 2017. Variation in Total Polyphenolic Contents, DNA Protective Potential and Antioxidant Capacity from Aqueous and Ethanol Extracts in Different Plant Parts of Hypericum perforatum L. Indian Journal of Pharmaceutical Education and Research 51: 1-7. https://doi.org/10.5530/ijper.51.2s.43.
  • Senol FS, Sekeroglu N, Gezici S, Kilic E, Orhan IE 2018. Neuroprotective potential of the fruit (acorn) from Quercus coccifera L. Turkish Journal of Agriculture and Forestry 42(2): 82-87. https://doi.org/10.3906/tar-1711-18.
  • Shida W, Tateishi H, Fujita M, Koga R, Radwan MO, Ciftci HI, Otsuka M, Husham AL-Saadi, D, Watanabe M, Gezici S, Wada M, Sekeroglu N, Watanabe T 2019. Anticancer activity of extract from twigs of Caucasian beech in Turkey. The Fifth International Symposium on Pharmaceutical and Biomedical Sciences (ISPBS-5), Cappadocia-Turkey, Oral presentation p: 29. www.ispbs.org.
  • Siegel RL, Miller KD, Jemal A 2018. Cancer statistics, 2018. CA: Cancer Journal for Clinicians 68(1): 7-30. https://doi.org/10.3322/caac.21442.
  • Sohretoglu D, Ekizoglu M, Kilic E, Sakar MK 2007. Antibacterial and antifungal activities of some Quercus species growing in Turkey. FABAD Journal of Pharmaceutical Sciences 32 (3):127–130.
  • Strober W 2015. Trypan blue exclusion test of cell viability. Current Protocols in Immunology 111(1): A3. B.1-A3. B.3. https://doi.org/ 10.1002/0471142735. ima03bs111.
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Sevgi Gezici 0000-0002-4856-0221

Publication Date December 31, 2019
Submission Date June 21, 2019
Acceptance Date August 1, 2019
Published in Issue Year 2019Cilt 22 (Ek Sayı 2)

Cite

APA Gezici, S. (2019). Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22, 374-381. https://doi.org/10.18016/ksutarimdoga.v22i49454.580285

Cited By

Oak trees (Quercus spp.) as a source of extracts with biological activities: A narrative review
Trends in Food Science & Technology
https://doi.org/10.1016/j.tifs.2021.01.029
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