Research Article
BibTex RIS Cite

Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking

Year 2018, Volume: 10 Issue: 3, 289 - 293, 23.10.2018
https://doi.org/10.18521/ktd.435349

Abstract

Background: DNA damage associated with passive
smoking has been shown in children. Severe DNA damage can change the apoptosis
process of the cell.  M30 and M65
biomarkers are markers showing the apoptosis process and can be quantitatively
measured. We aimed to determine M30 and M65 levels as apoptosis biomarkers in
children exposed to passive smoking.



Methods: A total of 79 children were included
in the study. Urine cotinine level was measured with the chemiluminescence
method and the levels of apoptosis biomarkers M30 and M65 were measured in
peripheral venous blood with PEVIVA/ALEXIS ELISA kits.



Results: The mean serum M30 level of the group
exposed to smoking was found to be significantly low compared to that of the
control group (p=0.01). The serum M65 value was not significant in either group
(p=0.1). No correlation was determined between the mean cotinine level and M30
(r=0.25, p=0.02) or M65 (r=0.16, p=0.15).



Conclusion: The results of this study showed that
the apoptosis biomarker M30 levels of the children exposed to passive smoking
were low compared to those of the control group which can be interpreted as
apoptosis inhibition in exposure to cigarette smoke. Further clinical studies
are needed.

References

  • De Sario M, Forastiere F, Viegi G et al. [Parental smoking and respiratory disorders in childhood]. Epidemiol Prev 2005; 29(2 Suppl): 52-56.
  • Dejmek J, Solansk y I, Podrazilova K, Sram RJ. The exposure of nonsmoking and smoking mothers to environmental tobacco smoke during different gestational phases and fetal growth. Environ Health Perspect 2002; 110(6): 601-6.
  • Norppa H, Bonassi S, Hansteen IL et al. Chromosomal aberrations and SCEs as biomarkers of cancer risk. Mutat Res 2006; 600(1-2): 37-45.
  • Rahman I, Adcock IM. Oxidative stress and redox regulation of lung inflammation in COPD. Eur Respir J 2006; 28(1): 219-42.
  • Siedlinski M, Postma DS, van Diemen CC et al. Lung function loss, smoking, vitamin C intake, and polymorphisms of the glutamate-cysteine ligase genes. Am J Respir Crit Care Med 2008; 178(1): 13-19.
  • Thatcher TH, Maggirwar SB, Baglole CJ et al. Aryl hydrocarbon receptor-deficient mice develop heightened inflammatory responses to cigarette smoke and endotoxin associated with rapid loss of the nuclear factor-kappaB component RelB. Am J Pathol 2007; 170(3): 855-64.
  • Yang SR, Wright J, Bauter M et al. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging. Am J Physiol Lung Cell Mol Physiol 2007; 292(2): 567-76.
  • Zalata A, Yahia S, El-Bakary A, Elsheikha HM. Increased DNA damage in children caused by passive smoking as assessed by comet assay and oxidative stress. Mutat Res 2007; 629(2): 140-7.
  • Shermatov K, Zeyrek D, Yildirim F et al. DNA damage in children exposed to secondhand cigarette smoke and its association with oxidative stress. Indian Pediatr 2012; 49(12): 958-62.
  • Michalska J, Motykiewicz G, Pendzich J et al. Measurement of cytogenetic endpoints in women environmentally exposed to air pollution. Mutat Res 1999; 445(2): 139-45.
  • Vineis P, Husgafvel-Pursiainen K. Air pollution and cancer: biomarker studies in human populations. Carcinogenesis 2005; 26(11): 1846-55.[12. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007; 35(4): 495-516.
  • Ku NO, Omary MB. Effect of mutation and phosphorylation of type I keratins on their caspase-mediated degradation. J Biol Chem 2001; 276(29): 26792-8.
  • Sundstrom BE, Stigbrand TI. Cytokeratins and tissue polypeptide antigen. Int J Biol Markers 1994; 9(2): 102-8.
  • Ueno T, Toi M, Biven K et al. Measurement of an apoptotic product in the sera of breast cancer patients. Eur J Cancer 2003; 39(6): 769-74.
  • Lee KS, Chung JY, Jung YJ et al. The significance of caspase-cleaved cytokeratin 18 in pleural effusion. Tuberc Respir Dis (Seoul) 2014; 76(1): 15-22.
  • Roth GA, Krenn C, Brunner M et al. Elevated serum levels of epithelial cell apoptosis-specific cytokeratin 18 neoepitope m30 in critically ill patients. Shock 2004; 22(3): 218-20.
  • Benowitz NL, Hukkanen J, Jacob P, 3rd. Nicotine chemistry, metabolism, kinetics and biomarkers. Handb Exp Pharmacol 2009(192); 29-60.
  • El-Sherbeeny NA, Nader MA, Attia GM, Ateyya H. Agmatine protects rat liver from nicotine-induced hepatic damage via antioxidative, antiapoptotic, and antifibrotic pathways. Naunyn Schmiedebergs Arch Pharmacol 2016; 389(12): 1341-51.
  • Cornelius MD, Goldschmidt L, Dempsey DA. Environmental tobacco smoke exposure in low-income 6-year-olds: parent report and urine cotinine measures. Nicotine Tob Res 2003; 5(3): 333-9.
  • Derauf C, Katz AR, Easa D. Agreement between maternal self-reported ethanol intake and tobacco use during pregnancy and meconium assays for fatty acid ethyl esters and cotinine. Am J Epidemiol 2003; 158(3): 705-9.
  • Cobanoglu N, Kiper N, Dilber E et al. Environmental tobacco smoke exposure and respiratory morbidity in children. Inhal Toxicol 2007; 19(9): 779-85.
  • Irvine L, Crombie IK, Clark RA et al. What determines levels of passive smoking in children with asthma? Thorax 1997; 52(9): 766-9.
  • Willers S, Attewell R, Bensryd I et al. Exposure to environmental tobacco smoke in the household and urinary cotinine excretion, heavy metals retention, and lung function. Arch Environ Health 1992; 47(5): 357-63.
  • Kim H, Liu X, Kobayashi T et al. Reversible cigarette smoke extract-induced DNA damage in human lung fibroblasts. Am J Respir Cell Mol Biol 2004; 31(5): 483-90.
  • Raveendran M, Wang J, Senthil D et al. Endogenous nitric oxide activation protects against cigarette smoking induced apoptosis in endothelial cells. FEBS Lett 2005; 579(3): 733-40.
  • Du H, Sun J, Chen Z et al. Cigarette smoke-induced failure of apoptosis resulting in enhanced neoplastic transformation in human bronchial epithelial cells. J Toxicol Environ Health A 2012; 75(12): 707-20.
  • Nakada T, Kiyotani K, Iwano S et al. Lung tumorigenesis promoted by anti-apoptotic effects of cotinine, a nicotine metabolite through activation of PI3K/Akt pathway. J Toxicol Sci 2012; 37(3): 555-63.
  • Tomita K, Caramori G, Lim S et al. Increased p21(CIP1/WAF1) and B cell lymphoma leukemia-x(L) expression and reduced apoptosis in alveolar macrophages from smokers. Am J Respir Crit Care Med 2002; 166(5): 724-31.
  • Wickenden JA, Clarke MC, Rossi AG et al. Cigarette smoke prevents apoptosis through inhibition of caspase activation and induces necrosis. Am J Respir Cell Mol Biol 2003; 29(5): 562-70.
  • Xu A, Prophete C, Chen LC et al. Interactive effect of cigarette smoke extract and world trade center dust particles on airway cell cytotoxicity. J Toxicol Environ Health A 2011; 74(14): 887-902.
  • Zhang T, Lu H, Shang X et al. Nicotine prevents the apoptosis induced by menadione in human lung cancer cells. Biochem Biophys Res Commun 2006; 342(3): 928-34.
  • Richens TR, Linderman DJ, Horstmann SA et al. Cigarette smoke impairs clearance of apoptotic cells through oxidant-dependent activation of RhoA. Am J Respir Crit Care Med 2009; 179(11): 1011-21.

Pasif Sigara İçen Çocuklarda Apotozis Biyobelirteci Olarak Serum M30 ve M65 Protein Düzeyleri

Year 2018, Volume: 10 Issue: 3, 289 - 293, 23.10.2018
https://doi.org/10.18521/ktd.435349

Abstract

Amaç: Pasif sigara içiciliğine bağlı DNA
hasarı çocuklarda gösterilmiştir. Ciddi DNA hasarı, hücrenin apoptoz sürecini
değiştirebilir. M30 ve M65 biyobelirteçleri apoptoz sürecini gösteren
belirteçlerdir ve kantitatif olarak ölçülebilir. Pasif sigara içiciliğine maruz
kalan çocuklarda M30 ve M65 düzeylerini apoptoz biyobelirteçleri olarak
belirlemeyi amaçladık.

Yöntemler: Çalışmaya toplam 79 çocuk dahil
edildi. İdrar kotinin düzeyi kemilüminesans yöntemiyle ölçüldü ve periferik
venöz kanda apoptoz biyobelirteçleri olan M30 ve M65 düzeyleri PEVIVA / ALEXIS
ELISA kitleri ile ölçüldü.

Bulgular: Sigaraya maruz kalan grubun ortalama
M30 düzeyi kontrol grubuna göre anlamlı derecede düşük bulundu (p = 0.01).
Serum M65 değeri her iki grupta da anlamlı değildi (p = 0.1). Ortalama kotinin
düzeyi ile M30 (r = 0.25, p = 0.02) veya M65 (r = 0.16, p = 0.15) arasında
korelasyon saptanmadı.







Sonuç: Bu çalışmanın sonuçları, pasif
sigara içiciliğine maruz kalan çocukların apoptoz biyobelirteçi olan M30
düzeylerinin, kontrol grubuna göre kıyaslandığında sigara dumanına maruziyette
apoptoz inhibisyonu olarak yorumlanabilecek şekilde düşük olduğunu gösterdi.
İleri klinik çalışmalara ihtiyaç duyulmaktadır.

References

  • De Sario M, Forastiere F, Viegi G et al. [Parental smoking and respiratory disorders in childhood]. Epidemiol Prev 2005; 29(2 Suppl): 52-56.
  • Dejmek J, Solansk y I, Podrazilova K, Sram RJ. The exposure of nonsmoking and smoking mothers to environmental tobacco smoke during different gestational phases and fetal growth. Environ Health Perspect 2002; 110(6): 601-6.
  • Norppa H, Bonassi S, Hansteen IL et al. Chromosomal aberrations and SCEs as biomarkers of cancer risk. Mutat Res 2006; 600(1-2): 37-45.
  • Rahman I, Adcock IM. Oxidative stress and redox regulation of lung inflammation in COPD. Eur Respir J 2006; 28(1): 219-42.
  • Siedlinski M, Postma DS, van Diemen CC et al. Lung function loss, smoking, vitamin C intake, and polymorphisms of the glutamate-cysteine ligase genes. Am J Respir Crit Care Med 2008; 178(1): 13-19.
  • Thatcher TH, Maggirwar SB, Baglole CJ et al. Aryl hydrocarbon receptor-deficient mice develop heightened inflammatory responses to cigarette smoke and endotoxin associated with rapid loss of the nuclear factor-kappaB component RelB. Am J Pathol 2007; 170(3): 855-64.
  • Yang SR, Wright J, Bauter M et al. Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging. Am J Physiol Lung Cell Mol Physiol 2007; 292(2): 567-76.
  • Zalata A, Yahia S, El-Bakary A, Elsheikha HM. Increased DNA damage in children caused by passive smoking as assessed by comet assay and oxidative stress. Mutat Res 2007; 629(2): 140-7.
  • Shermatov K, Zeyrek D, Yildirim F et al. DNA damage in children exposed to secondhand cigarette smoke and its association with oxidative stress. Indian Pediatr 2012; 49(12): 958-62.
  • Michalska J, Motykiewicz G, Pendzich J et al. Measurement of cytogenetic endpoints in women environmentally exposed to air pollution. Mutat Res 1999; 445(2): 139-45.
  • Vineis P, Husgafvel-Pursiainen K. Air pollution and cancer: biomarker studies in human populations. Carcinogenesis 2005; 26(11): 1846-55.[12. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007; 35(4): 495-516.
  • Ku NO, Omary MB. Effect of mutation and phosphorylation of type I keratins on their caspase-mediated degradation. J Biol Chem 2001; 276(29): 26792-8.
  • Sundstrom BE, Stigbrand TI. Cytokeratins and tissue polypeptide antigen. Int J Biol Markers 1994; 9(2): 102-8.
  • Ueno T, Toi M, Biven K et al. Measurement of an apoptotic product in the sera of breast cancer patients. Eur J Cancer 2003; 39(6): 769-74.
  • Lee KS, Chung JY, Jung YJ et al. The significance of caspase-cleaved cytokeratin 18 in pleural effusion. Tuberc Respir Dis (Seoul) 2014; 76(1): 15-22.
  • Roth GA, Krenn C, Brunner M et al. Elevated serum levels of epithelial cell apoptosis-specific cytokeratin 18 neoepitope m30 in critically ill patients. Shock 2004; 22(3): 218-20.
  • Benowitz NL, Hukkanen J, Jacob P, 3rd. Nicotine chemistry, metabolism, kinetics and biomarkers. Handb Exp Pharmacol 2009(192); 29-60.
  • El-Sherbeeny NA, Nader MA, Attia GM, Ateyya H. Agmatine protects rat liver from nicotine-induced hepatic damage via antioxidative, antiapoptotic, and antifibrotic pathways. Naunyn Schmiedebergs Arch Pharmacol 2016; 389(12): 1341-51.
  • Cornelius MD, Goldschmidt L, Dempsey DA. Environmental tobacco smoke exposure in low-income 6-year-olds: parent report and urine cotinine measures. Nicotine Tob Res 2003; 5(3): 333-9.
  • Derauf C, Katz AR, Easa D. Agreement between maternal self-reported ethanol intake and tobacco use during pregnancy and meconium assays for fatty acid ethyl esters and cotinine. Am J Epidemiol 2003; 158(3): 705-9.
  • Cobanoglu N, Kiper N, Dilber E et al. Environmental tobacco smoke exposure and respiratory morbidity in children. Inhal Toxicol 2007; 19(9): 779-85.
  • Irvine L, Crombie IK, Clark RA et al. What determines levels of passive smoking in children with asthma? Thorax 1997; 52(9): 766-9.
  • Willers S, Attewell R, Bensryd I et al. Exposure to environmental tobacco smoke in the household and urinary cotinine excretion, heavy metals retention, and lung function. Arch Environ Health 1992; 47(5): 357-63.
  • Kim H, Liu X, Kobayashi T et al. Reversible cigarette smoke extract-induced DNA damage in human lung fibroblasts. Am J Respir Cell Mol Biol 2004; 31(5): 483-90.
  • Raveendran M, Wang J, Senthil D et al. Endogenous nitric oxide activation protects against cigarette smoking induced apoptosis in endothelial cells. FEBS Lett 2005; 579(3): 733-40.
  • Du H, Sun J, Chen Z et al. Cigarette smoke-induced failure of apoptosis resulting in enhanced neoplastic transformation in human bronchial epithelial cells. J Toxicol Environ Health A 2012; 75(12): 707-20.
  • Nakada T, Kiyotani K, Iwano S et al. Lung tumorigenesis promoted by anti-apoptotic effects of cotinine, a nicotine metabolite through activation of PI3K/Akt pathway. J Toxicol Sci 2012; 37(3): 555-63.
  • Tomita K, Caramori G, Lim S et al. Increased p21(CIP1/WAF1) and B cell lymphoma leukemia-x(L) expression and reduced apoptosis in alveolar macrophages from smokers. Am J Respir Crit Care Med 2002; 166(5): 724-31.
  • Wickenden JA, Clarke MC, Rossi AG et al. Cigarette smoke prevents apoptosis through inhibition of caspase activation and induces necrosis. Am J Respir Cell Mol Biol 2003; 29(5): 562-70.
  • Xu A, Prophete C, Chen LC et al. Interactive effect of cigarette smoke extract and world trade center dust particles on airway cell cytotoxicity. J Toxicol Environ Health A 2011; 74(14): 887-902.
  • Zhang T, Lu H, Shang X et al. Nicotine prevents the apoptosis induced by menadione in human lung cancer cells. Biochem Biophys Res Commun 2006; 342(3): 928-34.
  • Richens TR, Linderman DJ, Horstmann SA et al. Cigarette smoke impairs clearance of apoptotic cells through oxidant-dependent activation of RhoA. Am J Respir Crit Care Med 2009; 179(11): 1011-21.
There are 32 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Kabil Shermatov This is me 0000-0001-8680-0719

Halil Kazanasmaz 0000-0003-4671-4028

Bulent Guzel This is me

Nazime Cebi This is me

Publication Date October 23, 2018
Acceptance Date September 27, 2018
Published in Issue Year 2018 Volume: 10 Issue: 3

Cite

APA Shermatov, K., Kazanasmaz, H., Guzel, B., Cebi, N. (2018). Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking. Konuralp Medical Journal, 10(3), 289-293. https://doi.org/10.18521/ktd.435349
AMA Shermatov K, Kazanasmaz H, Guzel B, Cebi N. Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking. Konuralp Medical Journal. October 2018;10(3):289-293. doi:10.18521/ktd.435349
Chicago Shermatov, Kabil, Halil Kazanasmaz, Bulent Guzel, and Nazime Cebi. “Levels of Serum M30 and M65 Proteins As Biomarkers of Apoptosis in Children Exposed to Passive Smoking”. Konuralp Medical Journal 10, no. 3 (October 2018): 289-93. https://doi.org/10.18521/ktd.435349.
EndNote Shermatov K, Kazanasmaz H, Guzel B, Cebi N (October 1, 2018) Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking. Konuralp Medical Journal 10 3 289–293.
IEEE K. Shermatov, H. Kazanasmaz, B. Guzel, and N. Cebi, “Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking”, Konuralp Medical Journal, vol. 10, no. 3, pp. 289–293, 2018, doi: 10.18521/ktd.435349.
ISNAD Shermatov, Kabil et al. “Levels of Serum M30 and M65 Proteins As Biomarkers of Apoptosis in Children Exposed to Passive Smoking”. Konuralp Medical Journal 10/3 (October 2018), 289-293. https://doi.org/10.18521/ktd.435349.
JAMA Shermatov K, Kazanasmaz H, Guzel B, Cebi N. Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking. Konuralp Medical Journal. 2018;10:289–293.
MLA Shermatov, Kabil et al. “Levels of Serum M30 and M65 Proteins As Biomarkers of Apoptosis in Children Exposed to Passive Smoking”. Konuralp Medical Journal, vol. 10, no. 3, 2018, pp. 289-93, doi:10.18521/ktd.435349.
Vancouver Shermatov K, Kazanasmaz H, Guzel B, Cebi N. Levels of Serum M30 and M65 Proteins as Biomarkers of Apoptosis in Children Exposed to Passive Smoking. Konuralp Medical Journal. 2018;10(3):289-93.