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Conributions to The Genetic Diversity of Turkish Morchella (Kuzugöbeği) with Using Molecular Techniques

Year 2020, Volume: 11 Issue: 2, 142 - 157, 28.10.2020

Fuat Bozok İsmail Keskinkılıç İlgaz Akata Mahmut Yarar Hatıra Taşkın

Abstract

Morchella genus called as “kuzugöbeği” in Turkey, is consumed extensively in many countries including Turkey and it always maintains its scientific interest. Systematic studies on the genus Morchella have continued, new species have also been identified and synonyms have been determined. In this study, phylogenetic and morphological assessment of a collection consisting 50 samples collected from different locations of Turkey were performed. In phylogenetic analysis, internal transcribed spacer (ITS rDNA) and 28S rDNA (LSU) gene regions were used. At the end of the study, Morchella dunensis, M. tridentina, M. importuna, M. eximia, M. dunalii, M. mediterraneensis and M. deliciosa have been identified based on these gene regions.

Keywords

Morchella Morel ITS rDNA 28S rDNA Turkey

References

  • Baroni, T. J., Michael, W., Beug, M. W., Cantrell, S. A., Teresa, A., Clements, T. A., Iturriaga, T., Læssøe, T., Holgado Rojas, M. E., Aguilar, F. M., Quispe, M. O., Lodge, D. J. & O’Donnell, K. (2018). Four new species of Morchella from the Americas. Mycologia, 116 1-17.
  • Boudier, J. L. É. (1887). Notice sur les discomycètes figurés dans les dessins inédits de Dunal conservés à la Faculté de Montpellier. Cottez.
  • Castañera, V., & Moreno, G. (1996). Una Morchella (Morchella esculenta forma dunensis f. nov.) frecuente en las dunas de Cantabria. Yesca. Revista Sociedad Micólogica de Cántabria, 8, 27.
  • Clowez, P. (1997). Morchella dunensis (Boud.) Clowez (stat. et comb. nov. ad int.). Une bonne espèce pour un mystère nomenclatural. Doc Mycol, 26(104), 13-20.
  • Clowez, P. (2012). Les morilles. Une nouvelle approche mondiale du genre Morchella. Bulletin de la Société Mycologique de France, 126 199-376.
  • Clowez, P., Alvarado, P., Becerra, M., Bilbao, T., & Moreau, P. A. (2014). Morchella fluvialis sp. nov.(Ascomycota, Pezizales): a new but widespread morel in Spain. Boletín de la Sociedad Micológica de Madrid, 38, 253-262.
  • Clowez, P., Bellanger, J. M., Romero De La Osa, L. & Moreau, P. A. (2015). Morchella palazonii sp. nov. (Ascomycota, Pezizales): une nouvelle morille méditerranéenne. Clé des Morchella sect. Morchella en Europe. Documents Mycologigues, XXXVI 71-84.
  • Clowez, P., Marcos Martinez, J., Sanjaume, R., Marques, G., Bellanger, J. M., & Moreau, P. A. (2020). A survey of half-free morels in Spain reveals a new species: Morchella iberica sp. nov. (Ascomycota, Pezizales). Ascomycete.org., 12(1). doi: 10.25664/art-0291.
  • Du, X. H., Zhao, Q., O’Donnell, K., Rooney, A. P., & Yang, Z. L. (2012a). Multigene molecular phylogenetics reveals true morels (Morchella) are especially species-rich in China. Fungal Genetics and Biology, 49(6), 455-469.
  • Du, X. H., Zhao, Q., Yang, Z. L., Hansen, K., Taşkın, H., Büyükalaca, S., Dewsbury, D., Moncalvo, J. M., Douhan, G. W., Robert, V. A. R. G., Crous, P. W. , Rehner, S. A., Rooney, A. P., Sink, S. & O’Donnell, K. (2012b). How well do ITS rDNA sequences differentiate species of true morels (Morchella)?. Mycologia 104 6 1351-1368.
  • Du, X. H., Wu, D. M., He, G. Q., Wei, W., Xu, N. & Li, T. L. (2019). Six new species and two new records of Morchella in China using phylogenetic and morphological analyses. Mycologia, 111 (5) 857-87.
  • Elliott, T. F., Bougher, N. L., O'Donnell, K. & Trappe, J. M. (2014). Morchella australiana sp. nov., an apparent Australian endemic from New South Wales and Victoria. Mycologia, 106 1 113-118.
  • Greene, D. F., Hesketh, M., & Pounden, E. (2010). Emergence of morel (Morchella) and pixie cup (Geopyxis carbonaria) ascocarps in response to the intensity of forest floor combustion during a wildfire. Mycologia, 102(4), 766-773.
  • Işiloğlu, M., Alli, H., Spooner, B. M., & Solak, M. H. (2010). Morchella anatolica (Ascomycota), a new species from southwestern Anatolia, Turkey. Mycologia, 102(2), 455-458.
  • Kuo, M., Dewsbury, D. R., O'Donnell, K., Carter, M. C., Rehner, S. A., Moore, J. D., Moncalvo, J. M., Canfield, S. A., Stephenson, S. L., Methven, A. S. & Volk, T. J. (2012). Taxonomic revision of true morels (Morchella) in Canada and the United States. Mycologia, 104 5 1159-1177.
  • Loizides, M. (2017). Morels: the story so far. Field Mycology, 18(2), 42-53.
  • Loizides, M., Alvarado, P., Clowez, P., Moreau, P. A., de la Osa, L. R., & Palazón, A. (2015). Morchella tridentina, M. rufobrunnea, and M. kakiicolor: a study of three poorly known Mediterranean morels, with nomenclatural updates in section Distantes. Mycological progress, 14(3), 1-18.
  • Loizides, M., Bellanger, J. M., Clowez, P., Richard, F., & Moreau, P. A. (2016). Combined phylogenetic and morphological studies of true morels (Pezizales, Ascomycota) in Cyprus reveal significant diversity, including Morchella arbutiphila and M. disparilis spp. nov. Mycological Progress, 15(4), 39.
  • Moreau, P. A., Hériveau, P., Bellanger, J. M., Courtecuisse, R., Fons, F., & Rapior, S. (2011). Redécouverte et typification des champignons de la région de Montpellier illustrés par Michel-Félix Dunal et Alire Raffeneau-Delile. Cryptogamie, Mycologie, 32(3), 255-276.
  • O’Donnell, K., Cigelnik, E., Weber, N. S. & Trappe, J. M. (1997). Phylogenetic relationships among ascomycetous truffles and true and false morels from 18S and 28S ribosomal DNA sequence analyses. Mycologia, 89 1 48-65.
  • O’Donnell, K., Rooney, A. P., Mills, G. L., Kuo, M., Weber, N. S. & Rehner, S. A. (2011). Phylogeny and historical biogeography of true morels (Morchella) reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic. Fungal Genet. Biol., 48 3 252-265.
  • Richard, F., Bellanger, J. M., Clowez, P., Hansen, H., O’Donnell, K., Urban, A., Sauve, M., Courtecuisse, R. & Moreau, P.A. (2015). True morels (Morchella, Pezizales) of Europe and North America: evolutionary relationships inferred from multilocus data and a unified taxonomy. Mycologia, 107 2 359-383.
  • Sambrook, J. F. & Russell, D. W. (2001).Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • Taşkın, H., Büyükalaca, S., Doğan, H. H., Rehner, S. A. & O’Donnell, K. (2010). A multigene molecular phylogenetic assessment of true morels (Morchella) in Turkey. Fungal Genet. Biol., 47 8 672-682.
  • Taşkın, H., Büyükalaca, S., Hansen, K. & O’Donnell, K. (2012). Multilocus phylogenetic analysis of true morels (Morchella) reveals high levels of endemics in Turkey relative to other regions of Europe. Mycologia, 104 2 446-461.
  • Taşkın, H., Doğan, H. H., Büyükalaca, S., Clowez, P., Moreau, P. A. & O’Donnell, K. (2016). Four new morel (Morchella) species in the Elata Subclade (sect. Distantes) from Turkey. Mycotaxon, 131 2 467-482.
  • Voitk, A., Burzynski, M., O’Donnell, K., Voitk, M. ve Marceau, A. (2014). Mel-36-preliminary description of a new morel species. Omphalina, 5 2 7-10.
  • Voitk, A., Beug, M. W., O’Donnell, K. & Burzynski, M. (2016). Two new species of true morels from Newfoundland and Labrador: cosmopolitan Morchella eohespera and parochial M. laurentiana. Mycologia, 108 1 31-37.
  • White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR–Protocols and Applications–A Laboratory Manual. San Diego: Academic Press, ss: 315-322.

Moleküler Yöntemlerin Kullanımı İle Türkiye Morchella (Kuzugöbeği) Genetik Çeşitliliğine Katkılar

Year 2020, Volume: 11 Issue: 2, 142 - 157, 28.10.2020

Fuat Bozok İsmail Keskinkılıç İlgaz Akata Mahmut Yarar Hatıra Taşkın

Abstract

Türkiye’de “kuzugöbeği” olarak bilinen Morchella cinsi, Türkiye’nin de içinde bulunduğu birçok ülkede yoğun olarak tüketilmekte ve bilimsel olarak da üzerindeki ilgiyi her zaman korumaktadır. Bu mantar cinsinde yapılan sistematik çalışmalar popülerliğini sürdürmekte, yeni türler tanımlanmakta ve sinonimler belirlenmektedir. Sunulan bu çalışmada da Türkiye’nin farklı lokasyonlarından toplanmış ve 50 örnekten oluşmuş bir koleksiyonun morfolojik ve filogenetik değerlendirilmesi yapılmıştır. Filogenetik analizlerde, transkripsiyonu yapılamayan (ITS rDNA) ve 28S rDNA (LSU) gen bölgeleri kullanılmıştır. Çalışma sonunda, bu gen bölgelerine göre, Morchella dunensis, M. tridentina, M. importuna, M. eximia, M. dunalii, M. mediterraneensis ve M. deliciosa türleri belirlenmiştir.

Keywords

Morchella kuzugöbeği ITS rDNA 28S rDNA Türkiye

References

  • Baroni, T. J., Michael, W., Beug, M. W., Cantrell, S. A., Teresa, A., Clements, T. A., Iturriaga, T., Læssøe, T., Holgado Rojas, M. E., Aguilar, F. M., Quispe, M. O., Lodge, D. J. & O’Donnell, K. (2018). Four new species of Morchella from the Americas. Mycologia, 116 1-17.
  • Boudier, J. L. É. (1887). Notice sur les discomycètes figurés dans les dessins inédits de Dunal conservés à la Faculté de Montpellier. Cottez.
  • Castañera, V., & Moreno, G. (1996). Una Morchella (Morchella esculenta forma dunensis f. nov.) frecuente en las dunas de Cantabria. Yesca. Revista Sociedad Micólogica de Cántabria, 8, 27.
  • Clowez, P. (1997). Morchella dunensis (Boud.) Clowez (stat. et comb. nov. ad int.). Une bonne espèce pour un mystère nomenclatural. Doc Mycol, 26(104), 13-20.
  • Clowez, P. (2012). Les morilles. Une nouvelle approche mondiale du genre Morchella. Bulletin de la Société Mycologique de France, 126 199-376.
  • Clowez, P., Alvarado, P., Becerra, M., Bilbao, T., & Moreau, P. A. (2014). Morchella fluvialis sp. nov.(Ascomycota, Pezizales): a new but widespread morel in Spain. Boletín de la Sociedad Micológica de Madrid, 38, 253-262.
  • Clowez, P., Bellanger, J. M., Romero De La Osa, L. & Moreau, P. A. (2015). Morchella palazonii sp. nov. (Ascomycota, Pezizales): une nouvelle morille méditerranéenne. Clé des Morchella sect. Morchella en Europe. Documents Mycologigues, XXXVI 71-84.
  • Clowez, P., Marcos Martinez, J., Sanjaume, R., Marques, G., Bellanger, J. M., & Moreau, P. A. (2020). A survey of half-free morels in Spain reveals a new species: Morchella iberica sp. nov. (Ascomycota, Pezizales). Ascomycete.org., 12(1). doi: 10.25664/art-0291.
  • Du, X. H., Zhao, Q., O’Donnell, K., Rooney, A. P., & Yang, Z. L. (2012a). Multigene molecular phylogenetics reveals true morels (Morchella) are especially species-rich in China. Fungal Genetics and Biology, 49(6), 455-469.
  • Du, X. H., Zhao, Q., Yang, Z. L., Hansen, K., Taşkın, H., Büyükalaca, S., Dewsbury, D., Moncalvo, J. M., Douhan, G. W., Robert, V. A. R. G., Crous, P. W. , Rehner, S. A., Rooney, A. P., Sink, S. & O’Donnell, K. (2012b). How well do ITS rDNA sequences differentiate species of true morels (Morchella)?. Mycologia 104 6 1351-1368.
  • Du, X. H., Wu, D. M., He, G. Q., Wei, W., Xu, N. & Li, T. L. (2019). Six new species and two new records of Morchella in China using phylogenetic and morphological analyses. Mycologia, 111 (5) 857-87.
  • Elliott, T. F., Bougher, N. L., O'Donnell, K. & Trappe, J. M. (2014). Morchella australiana sp. nov., an apparent Australian endemic from New South Wales and Victoria. Mycologia, 106 1 113-118.
  • Greene, D. F., Hesketh, M., & Pounden, E. (2010). Emergence of morel (Morchella) and pixie cup (Geopyxis carbonaria) ascocarps in response to the intensity of forest floor combustion during a wildfire. Mycologia, 102(4), 766-773.
  • Işiloğlu, M., Alli, H., Spooner, B. M., & Solak, M. H. (2010). Morchella anatolica (Ascomycota), a new species from southwestern Anatolia, Turkey. Mycologia, 102(2), 455-458.
  • Kuo, M., Dewsbury, D. R., O'Donnell, K., Carter, M. C., Rehner, S. A., Moore, J. D., Moncalvo, J. M., Canfield, S. A., Stephenson, S. L., Methven, A. S. & Volk, T. J. (2012). Taxonomic revision of true morels (Morchella) in Canada and the United States. Mycologia, 104 5 1159-1177.
  • Loizides, M. (2017). Morels: the story so far. Field Mycology, 18(2), 42-53.
  • Loizides, M., Alvarado, P., Clowez, P., Moreau, P. A., de la Osa, L. R., & Palazón, A. (2015). Morchella tridentina, M. rufobrunnea, and M. kakiicolor: a study of three poorly known Mediterranean morels, with nomenclatural updates in section Distantes. Mycological progress, 14(3), 1-18.
  • Loizides, M., Bellanger, J. M., Clowez, P., Richard, F., & Moreau, P. A. (2016). Combined phylogenetic and morphological studies of true morels (Pezizales, Ascomycota) in Cyprus reveal significant diversity, including Morchella arbutiphila and M. disparilis spp. nov. Mycological Progress, 15(4), 39.
  • Moreau, P. A., Hériveau, P., Bellanger, J. M., Courtecuisse, R., Fons, F., & Rapior, S. (2011). Redécouverte et typification des champignons de la région de Montpellier illustrés par Michel-Félix Dunal et Alire Raffeneau-Delile. Cryptogamie, Mycologie, 32(3), 255-276.
  • O’Donnell, K., Cigelnik, E., Weber, N. S. & Trappe, J. M. (1997). Phylogenetic relationships among ascomycetous truffles and true and false morels from 18S and 28S ribosomal DNA sequence analyses. Mycologia, 89 1 48-65.
  • O’Donnell, K., Rooney, A. P., Mills, G. L., Kuo, M., Weber, N. S. & Rehner, S. A. (2011). Phylogeny and historical biogeography of true morels (Morchella) reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic. Fungal Genet. Biol., 48 3 252-265.
  • Richard, F., Bellanger, J. M., Clowez, P., Hansen, H., O’Donnell, K., Urban, A., Sauve, M., Courtecuisse, R. & Moreau, P.A. (2015). True morels (Morchella, Pezizales) of Europe and North America: evolutionary relationships inferred from multilocus data and a unified taxonomy. Mycologia, 107 2 359-383.
  • Sambrook, J. F. & Russell, D. W. (2001).Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • Taşkın, H., Büyükalaca, S., Doğan, H. H., Rehner, S. A. & O’Donnell, K. (2010). A multigene molecular phylogenetic assessment of true morels (Morchella) in Turkey. Fungal Genet. Biol., 47 8 672-682.
  • Taşkın, H., Büyükalaca, S., Hansen, K. & O’Donnell, K. (2012). Multilocus phylogenetic analysis of true morels (Morchella) reveals high levels of endemics in Turkey relative to other regions of Europe. Mycologia, 104 2 446-461.
  • Taşkın, H., Doğan, H. H., Büyükalaca, S., Clowez, P., Moreau, P. A. & O’Donnell, K. (2016). Four new morel (Morchella) species in the Elata Subclade (sect. Distantes) from Turkey. Mycotaxon, 131 2 467-482.
  • Voitk, A., Burzynski, M., O’Donnell, K., Voitk, M. ve Marceau, A. (2014). Mel-36-preliminary description of a new morel species. Omphalina, 5 2 7-10.
  • Voitk, A., Beug, M. W., O’Donnell, K. & Burzynski, M. (2016). Two new species of true morels from Newfoundland and Labrador: cosmopolitan Morchella eohespera and parochial M. laurentiana. Mycologia, 108 1 31-37.
  • White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR–Protocols and Applications–A Laboratory Manual. San Diego: Academic Press, ss: 315-322.
There are 29 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Fuat Bozok 0000-0002-9370-7712

İsmail Keskinkılıç This is me 0000-0001-8642-9438

İlgaz Akata 0000-0002-1731-1302

Mahmut Yarar 0000-0003-3991-5649

Hatıra Taşkın 0000-0002-1784-4731

Publication Date October 28, 2020
Published in Issue Year 2020 Volume: 11 Issue: 2

Cite

APA Bozok, F., Keskinkılıç, İ., Akata, İ., Yarar, M., et al. (2020). Moleküler Yöntemlerin Kullanımı İle Türkiye Morchella (Kuzugöbeği) Genetik Çeşitliliğine Katkılar. Mantar Dergisi, 11(2), 142-157. https://doi.org/10.30708/mantar.734871
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