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‘Bayrampaşa’ enginarının (Cynara scolymus L.) in vitro köklenmesi üzerine bir protokol

Year 2019, Volume: 32 Issue: 2, 129 - 134, 01.08.2019
https://doi.org/10.29136/mediterranean.559194

Abstract

Asteraceae (Compositae) familyasında yer
alan enginar, dünyada oldukça geniş bir alanda yetiştiriciliği yapılmakta ve
içerdiği biyoaktif bileşenlerden dolayı fonksiyonel gıda olarak görülmektedir.
Enginarın doku kültürü yoluyla üretimi dikkate değer avantajlar sunmaktadır.
Öte yandan enginar doku kültürü çalışmalarında karşılaşılan en önemli
problemlerden biri
in vitro
köklenmedir. Bu nedenle bu çalışmanın temel amacı enginarın
in vitro köklenmesi üzerine etkili bir
protokol geliştirmektir. Bu amaçla, 3 alt kültür aşamasını içeren başarılı bir
mikroçoğaltım sürecinin ardından, iyi gelişme göstermiş olan bitkicikler
seçilmiş ve çalışmada kullanılan 10 farklı besi ortamı kombinasyonunda (kontrol
grubu dahil) köklendirilmiştir. Besi ortamları arasındaki farklılıklar, 10.0 mg
l
-1 indol asetik asit (IAA), 6.0 mg l-1 indol butirik
asit (IBA), 5.0 mg l
-1 gibberellik asit (GA3), 0.5 mg l-1
naftalen asetik asit (NAA) gibi farklı büyüme düzenleyicileri ve aktif kömür
(0, 1.0 ve 2.0 g l
-1) eklenerek sağlanmıştır. Bitkiciklerin
gelişimleri 15 gün aralıklarla gözlemlenmiş ve kaydedilmiştir. Çalışmadan elde
edilen verilere göre, mikroçoğaltım aşamasını takiben köklenme bakımından IAA
(10.0 mg l
-1) ve 1.0 g l-1 aktif kömür içeren besi ortamı
kombinasyonu en iyi sonuçları vermiştir.



References

  • Alp HA (2008) Enginarda Doku Kültürü Koşullarının İyileştirilmesine Yönelik Çalışmalar. Yüksek Lisans Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir.
  • Ancora G, Belli-Donini ML, Cuozzo L (1981) Globe artichoke plants obtained from shoot apices through rapid in vitro micropropagation. Scientia Hortic. 14: 207-213.
  • Bedini L, Lucchesini M, Bertozzi F, Graifenberg A (2012) Plant Tissue Cultures from Four Tuscan Globe Artichoke Cultivars. Cent. Eur. J. Biol. 7: 680-689. doi: 10.2478/s11535-012-0064-x.
  • Benoit H, Ducreux G (1981) Etude de quelques aspects de la multiplication végétative in vitro de l’artichaut (Cynara scolymus L.). Agronomie 1: 225-230.
  • Bianco VV (2005) Present situation and future potential of artichoke in the Mediterranean basin. Acta Hort. 681: 39-55.
  • Bigot C, Foury C (1984) In Vitro Propagation of Globe Artichoke (Cynara scolymus L.) from Seedlings: Field Comparison of Some Clones with Their Parent Lines. Agronomie 4: 699-710.
  • Brutti C, Apostolo NM, Ferrarotti SA, LLOrente BE, Krymkiewicz N. (2000) Micropropagation of Cynara scolymus L. employing cyclodestrins to promote rhizogenesis. Sci. Hort. 83: 1-10.
  • Calabrese N (2009) AA.VV. Il carciofo e il cardo, coordinamento scientifico N. Calabrese. Collana Coltura&Cultura, Ed. Script Bologna, Impianto 168-171.
  • Cavallaro V, Castiglione V, Avola G, Finocchiaro E (2004) Influence of different substrates on the in vitro rhizogenesis process of early artichoke [Cynara cardunculus L. subsp. scolymus (L.) Heg], Acta Hort. ISHS 660: 267-272.
  • Ciancolini A (2012) CHARACTERIZATION AND SELECTION OF GLOBE ARTICHOKE AND CARDOON GERMPLASM FOR BIOMASS, FOOD AND BIOCOMPOUND PRODUCTION. PhD thesis, Università degli Studi della Tuscia, Italy and Institut National Polytechnique de Toulouse, France.
  • De Leo P, Greco B (1976) New technique of artichoke propagation: in vitro culture of apical meristems [Nuova tecnica di propagazione del carciofo: coltura in vitro di meristemi apicali], In: Atti 2nd Congr. Int. Sul Carciofo, Bari. Ed. Minerva Medica Torino, 657-667.
  • Draoui N, Ghorbel A, Kchouk ME (1993) In vitro culture of globe artichoke (Cynara scolymus L.) in Tunisia: utilization of vitro methods in artichoke improvement. Agricoltura Mediterranea 123: 139–145.
  • El Boullani R, Elmoshli A, El Finti A, El Mousadik A, Serghini MA (2012) Improved in vitro Micropropagation of Artichoke (Cynara cardunculus var. scolymus L.). European Journal of Scientific Research 80: 430-436.
  • El Senousy AS, Farag MA, Al-Mahdy DA, Wessjohann LA (2014) Developmental changes in leaf phenolics composition from three artichoke cvs. (Cynara scolymus) as determined via UHPLC–MS and chemometrics. Phytochemistry 108: 67–76.
  • Ercan N (2016) Effects of Various Growth Regulators on in vitro Rooting of Globe Artichoke (Cynara scolymus L.). Journal of Agricultural Science and Technology A 6: 335-340. doi: 10.17265/2161-6256/2016.05.005.
  • Falco BD, Incerti G, Amato M, Lanzotti V (2015) Artichoke: botanical, agronomical, phytochemical, and pharmacological overview. Phytochem Rev 14: 993–1018. doi: 10.1007/s11101-015-9428-y.
  • Farag MA, El-Ahmady S, Alian F, Wessjohann LA (2013) Metabolomics driven analysis of artichoke leaf and its commercial products via UHPLC–q-TOF-MS. Phytochemistry 95: 177–187.
  • Fratianni F, Tucci M, De Palma M, Pepe R, Nazzaro F (2007) Polyphenolic composition in different parts of some cultivars of globe artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori). Food Chemistry 104: 1282-1286.
  • Harbaoui Y, Smaijin G, Welvaert W, Debergh P (1982) Assainissement viral de l’artichaut (Cynara scolimus L.) par la culture in vitro d’apex méristématiques. Phytopathologie Mediterrané 21: 15–19 (in French).
  • Heywood VH (1978) Flowering Plants of the World. Oxford University Press, Oxford, London.
  • Iapichino G (1996) Micropropagation of Globe Artichoke (Cynara scolymus L.) from underground dormant buds (ovoli). In Vitro Cell. Dev. Biol. Plant 32: 249-252.
  • Klein B, Bopp M (1971) Effect of activated charcoal in agar on the culture of lower plants. Nature 230:474.
  • Lanteri S, Portis E (2008) Globe artichoke and Cardoon. In Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Prohens J. And Nuez F. (Eds.) 428 Springer 49-74.
  • Lattanzio V, Kroon PA, Linsalata V, Cardinali A (2009) Globe artichoke: A functional food and a source of nutraceutical ingredients. Journal of Functional Foods 1: 131-144.
  • Llorach R, Espin JC, Tomás Barberán FA, Ferreres F (2002) Artichoke (Cynara scolymus L.) Byproducts as a Potential Source of Health-Promoting Antioxidant Phenolics. Journal of Agricultural and Food Chemistry 50: 3458-3464.
  • López-Pérez AJ, Martínez JA (2015) In Vitro Root Induction Improvement by Culture in Darkness for Different Globe Artichoke Cultivars. In Vitro Cell. Dev. Biol. Plant 51: 160-165. doi: 10.1007/s11627-015-9667-z.
  • Lucchesini M, Mensuali-Sodi A, Massai R, Gucci R (2001) Development of autotrophy and tolerance to acclimatization of Myrtus communis transplants cultured in vitro under different aeration. Biologia Plantarum 44: 167-174.
  • Marras F, Foddai A, Fiori M (1985) La selezione sanitaria del carciofo “Spinoso Sardo”. Informatore fitopatológico 35: 47–50 (in Italian).
  • Morzadec JM, Hourmant A (1997) In vitro rooting improvement of globe artichoke (cv. Camus de Bretagne) by GA3. Sci. Hortic. 72: 59-62.
  • Murashige T, Skoog FA (1962) A Revised Medium for a Rapid Growth and Bio Assays with Tobacco Tissues Cultures. Physiologia Plantarum 15: 473-97.
  • Pecaut P, Dumas R, De Vaulx R, Lot H (1983) Virus free clone of globe artichoke (Cynara scolymus) obtained after in vitro propagation. Acta Hort. 131: 303-309.
  • Rossi V, De Paoli G (1992) Micropropagation of Artichoke (Cynara scolymus), In: Bajaj Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry Vol 19. High- Tech and Micropropagation III, Spinger- Verlag, Berlin Heidelberg, Germany.
  • Ruiz-Aceituno L, García-Sarrió MJ, Alonso-Rodriguez B, Ramos L, Sanz ML (2016) Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction. Food Chemistry 196: 1156–1162.
  • Tavazza R, Papacchioli V, Ancora G (2004) An improved medium for in vitro propagation of globe artichoke (Cynara scolymus L.) cv. Spinoso sardo, Acta Hort. 660: 91-97.

A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.)

Year 2019, Volume: 32 Issue: 2, 129 - 134, 01.08.2019
https://doi.org/10.29136/mediterranean.559194

Abstract



Artichoke, belongs to
the family
Asteraceae (Compositae), is cultivated in a very
wide area in the world and regarded as a functional food due to the bioactive
components. The propagation of artichoke via tissue culture offers considerable
advantages. However one of the most important problem is
in vitro rooting. Therefore, the main purpose of the present study
was to develop an effective protocol on
in
vitro
rooting of ‘Bayrampaşa’ cultivar which is one of the important local
artichoke cultivar. To serve the purpose, after a successful micropropagation
process, involving 3 subculture stages, well-developed plantlets were selected
and rooted in different media compositions as ten different media including
control group were used in present study. The differences between the media
were provided by adding different growth regulators such as
10.0 mg l-1 indole-3-acetic
acid (IAA),
6.0 mg l-1 indole butyric acid (IBA), 5.0 mg l-1 gibberellic acid (GA3), 0.5
mg l
-1 naphthaleneacetic
acid (NAA) and activated charcoal (0, 1.0 and 2.0 g l
-1).
Developments of plantlets were observed and recorded at 15 days intervals.
According to the results obtained during the study, the medium containing IAA
(10.0 mg l
-1) and 1.0 g l-1 of activated charcoal gave
the best results in terms of rooting after micropropagation.




References

  • Alp HA (2008) Enginarda Doku Kültürü Koşullarının İyileştirilmesine Yönelik Çalışmalar. Yüksek Lisans Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir.
  • Ancora G, Belli-Donini ML, Cuozzo L (1981) Globe artichoke plants obtained from shoot apices through rapid in vitro micropropagation. Scientia Hortic. 14: 207-213.
  • Bedini L, Lucchesini M, Bertozzi F, Graifenberg A (2012) Plant Tissue Cultures from Four Tuscan Globe Artichoke Cultivars. Cent. Eur. J. Biol. 7: 680-689. doi: 10.2478/s11535-012-0064-x.
  • Benoit H, Ducreux G (1981) Etude de quelques aspects de la multiplication végétative in vitro de l’artichaut (Cynara scolymus L.). Agronomie 1: 225-230.
  • Bianco VV (2005) Present situation and future potential of artichoke in the Mediterranean basin. Acta Hort. 681: 39-55.
  • Bigot C, Foury C (1984) In Vitro Propagation of Globe Artichoke (Cynara scolymus L.) from Seedlings: Field Comparison of Some Clones with Their Parent Lines. Agronomie 4: 699-710.
  • Brutti C, Apostolo NM, Ferrarotti SA, LLOrente BE, Krymkiewicz N. (2000) Micropropagation of Cynara scolymus L. employing cyclodestrins to promote rhizogenesis. Sci. Hort. 83: 1-10.
  • Calabrese N (2009) AA.VV. Il carciofo e il cardo, coordinamento scientifico N. Calabrese. Collana Coltura&Cultura, Ed. Script Bologna, Impianto 168-171.
  • Cavallaro V, Castiglione V, Avola G, Finocchiaro E (2004) Influence of different substrates on the in vitro rhizogenesis process of early artichoke [Cynara cardunculus L. subsp. scolymus (L.) Heg], Acta Hort. ISHS 660: 267-272.
  • Ciancolini A (2012) CHARACTERIZATION AND SELECTION OF GLOBE ARTICHOKE AND CARDOON GERMPLASM FOR BIOMASS, FOOD AND BIOCOMPOUND PRODUCTION. PhD thesis, Università degli Studi della Tuscia, Italy and Institut National Polytechnique de Toulouse, France.
  • De Leo P, Greco B (1976) New technique of artichoke propagation: in vitro culture of apical meristems [Nuova tecnica di propagazione del carciofo: coltura in vitro di meristemi apicali], In: Atti 2nd Congr. Int. Sul Carciofo, Bari. Ed. Minerva Medica Torino, 657-667.
  • Draoui N, Ghorbel A, Kchouk ME (1993) In vitro culture of globe artichoke (Cynara scolymus L.) in Tunisia: utilization of vitro methods in artichoke improvement. Agricoltura Mediterranea 123: 139–145.
  • El Boullani R, Elmoshli A, El Finti A, El Mousadik A, Serghini MA (2012) Improved in vitro Micropropagation of Artichoke (Cynara cardunculus var. scolymus L.). European Journal of Scientific Research 80: 430-436.
  • El Senousy AS, Farag MA, Al-Mahdy DA, Wessjohann LA (2014) Developmental changes in leaf phenolics composition from three artichoke cvs. (Cynara scolymus) as determined via UHPLC–MS and chemometrics. Phytochemistry 108: 67–76.
  • Ercan N (2016) Effects of Various Growth Regulators on in vitro Rooting of Globe Artichoke (Cynara scolymus L.). Journal of Agricultural Science and Technology A 6: 335-340. doi: 10.17265/2161-6256/2016.05.005.
  • Falco BD, Incerti G, Amato M, Lanzotti V (2015) Artichoke: botanical, agronomical, phytochemical, and pharmacological overview. Phytochem Rev 14: 993–1018. doi: 10.1007/s11101-015-9428-y.
  • Farag MA, El-Ahmady S, Alian F, Wessjohann LA (2013) Metabolomics driven analysis of artichoke leaf and its commercial products via UHPLC–q-TOF-MS. Phytochemistry 95: 177–187.
  • Fratianni F, Tucci M, De Palma M, Pepe R, Nazzaro F (2007) Polyphenolic composition in different parts of some cultivars of globe artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori). Food Chemistry 104: 1282-1286.
  • Harbaoui Y, Smaijin G, Welvaert W, Debergh P (1982) Assainissement viral de l’artichaut (Cynara scolimus L.) par la culture in vitro d’apex méristématiques. Phytopathologie Mediterrané 21: 15–19 (in French).
  • Heywood VH (1978) Flowering Plants of the World. Oxford University Press, Oxford, London.
  • Iapichino G (1996) Micropropagation of Globe Artichoke (Cynara scolymus L.) from underground dormant buds (ovoli). In Vitro Cell. Dev. Biol. Plant 32: 249-252.
  • Klein B, Bopp M (1971) Effect of activated charcoal in agar on the culture of lower plants. Nature 230:474.
  • Lanteri S, Portis E (2008) Globe artichoke and Cardoon. In Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Prohens J. And Nuez F. (Eds.) 428 Springer 49-74.
  • Lattanzio V, Kroon PA, Linsalata V, Cardinali A (2009) Globe artichoke: A functional food and a source of nutraceutical ingredients. Journal of Functional Foods 1: 131-144.
  • Llorach R, Espin JC, Tomás Barberán FA, Ferreres F (2002) Artichoke (Cynara scolymus L.) Byproducts as a Potential Source of Health-Promoting Antioxidant Phenolics. Journal of Agricultural and Food Chemistry 50: 3458-3464.
  • López-Pérez AJ, Martínez JA (2015) In Vitro Root Induction Improvement by Culture in Darkness for Different Globe Artichoke Cultivars. In Vitro Cell. Dev. Biol. Plant 51: 160-165. doi: 10.1007/s11627-015-9667-z.
  • Lucchesini M, Mensuali-Sodi A, Massai R, Gucci R (2001) Development of autotrophy and tolerance to acclimatization of Myrtus communis transplants cultured in vitro under different aeration. Biologia Plantarum 44: 167-174.
  • Marras F, Foddai A, Fiori M (1985) La selezione sanitaria del carciofo “Spinoso Sardo”. Informatore fitopatológico 35: 47–50 (in Italian).
  • Morzadec JM, Hourmant A (1997) In vitro rooting improvement of globe artichoke (cv. Camus de Bretagne) by GA3. Sci. Hortic. 72: 59-62.
  • Murashige T, Skoog FA (1962) A Revised Medium for a Rapid Growth and Bio Assays with Tobacco Tissues Cultures. Physiologia Plantarum 15: 473-97.
  • Pecaut P, Dumas R, De Vaulx R, Lot H (1983) Virus free clone of globe artichoke (Cynara scolymus) obtained after in vitro propagation. Acta Hort. 131: 303-309.
  • Rossi V, De Paoli G (1992) Micropropagation of Artichoke (Cynara scolymus), In: Bajaj Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry Vol 19. High- Tech and Micropropagation III, Spinger- Verlag, Berlin Heidelberg, Germany.
  • Ruiz-Aceituno L, García-Sarrió MJ, Alonso-Rodriguez B, Ramos L, Sanz ML (2016) Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction. Food Chemistry 196: 1156–1162.
  • Tavazza R, Papacchioli V, Ancora G (2004) An improved medium for in vitro propagation of globe artichoke (Cynara scolymus L.) cv. Spinoso sardo, Acta Hort. 660: 91-97.
There are 34 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Makaleler
Authors

Tugce Ozsan 0000-0002-3265-6886

Ahmet Naci Onus 0000-0001-8615-1480

Publication Date August 1, 2019
Submission Date April 30, 2019
Published in Issue Year 2019 Volume: 32 Issue: 2

Cite

APA Ozsan, T., & Onus, A. N. (2019). A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.). Mediterranean Agricultural Sciences, 32(2), 129-134. https://doi.org/10.29136/mediterranean.559194
AMA Ozsan T, Onus AN. A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.). Mediterranean Agricultural Sciences. August 2019;32(2):129-134. doi:10.29136/mediterranean.559194
Chicago Ozsan, Tugce, and Ahmet Naci Onus. “A Protocol on in Vitro Rooting of ‘Bayrampaşa’ Artichoke (Cynara Scolymus L.)”. Mediterranean Agricultural Sciences 32, no. 2 (August 2019): 129-34. https://doi.org/10.29136/mediterranean.559194.
EndNote Ozsan T, Onus AN (August 1, 2019) A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.). Mediterranean Agricultural Sciences 32 2 129–134.
IEEE T. Ozsan and A. N. Onus, “A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.)”, Mediterranean Agricultural Sciences, vol. 32, no. 2, pp. 129–134, 2019, doi: 10.29136/mediterranean.559194.
ISNAD Ozsan, Tugce - Onus, Ahmet Naci. “A Protocol on in Vitro Rooting of ‘Bayrampaşa’ Artichoke (Cynara Scolymus L.)”. Mediterranean Agricultural Sciences 32/2 (August 2019), 129-134. https://doi.org/10.29136/mediterranean.559194.
JAMA Ozsan T, Onus AN. A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.). Mediterranean Agricultural Sciences. 2019;32:129–134.
MLA Ozsan, Tugce and Ahmet Naci Onus. “A Protocol on in Vitro Rooting of ‘Bayrampaşa’ Artichoke (Cynara Scolymus L.)”. Mediterranean Agricultural Sciences, vol. 32, no. 2, 2019, pp. 129-34, doi:10.29136/mediterranean.559194.
Vancouver Ozsan T, Onus AN. A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.). Mediterranean Agricultural Sciences. 2019;32(2):129-34.

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