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Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması

Year 2021, Volume: 34 Issue: 1, 161 - 167, 01.04.2021
https://doi.org/10.29136/mediterranean.796773

Abstract

Bu çalışmada Simental sığır ırkına ait toplam 202 hayvanda STAT5A-AvaI ve MYF5-TaqI polimorfizmleri ile günlük ve toplam süt verimi arasındaki ilişkinin araştırılması amaçlanmıştır. PZR tekniği ile çoğaltılan 215 bç uzunluğundaki STAT5A gen bölgesi AvaI restriksiyon enzimi ile kesilirken, 512 bç uzunluğundaki MYF5 gen bölgesi TaqI enzimi ile kesilmiştir. Belirlenen STAT5A-AvaI ve MYF5-TaqI genotipleri ile günlük ve laktasyon süt verimleri arasındaki ilişki Tek Yönlü Varyans Analizi (ANOVA) ile değerlendirilmiştir. Bu çalışmada incelenen Simental popülasyonunun STAT5A-AvaI ve MYF5-TaqI polimorfizmi yönünden Hardy-Weinberg (H-W) dengesinde olduğu belirlenmiştir. Çalışma sonunda incelenen Simental ırkı sağmal ineklerde STAT5A-AvaI polimorfizmi yönünden CT genotipli sığırların hem birinci hemde ikinci laktasyonda diğer genotiplere göre daha yüksek süt verimine sahip oldukları belirlenmiştir. Buna karşın bu çalışmada incelenen Simental ırkı ineklerde MYF5-TaqI polimorfizmi ile günlük ve toplam laktasyon süt verimleri arasında ilişki olmadığı belirlenmiştir. Sonuç olarak, STAT5A-AvaI polimorfizminin, Simental sığır ırkında günlük ve toplam laktasyon süt verimlerinin iyileştirilmesi amacıyla yapılacak ıslah çalışmalarında kullanılabilme potansiyeline sahip olduğu düşünülmektedir.

Supporting Institution

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

TYL-2019-8802

Thanks

Bu çalışmayı TYL-2019-8802 proje kodu ile destekleyen Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi’ne teşekkür ederiz.

References

  • Ağaoğlu ÖK, Akyüz B, Zeytünlü E, Ağaoğlu AR (2020) Investigation of G+ 265C and G-1539A single nucleotide polymorphisms of toll-like receptor 4 gene (TLR4) in some cattle breeds raised in Turkey. SlovenianVeterinary Research 57(1): 25-31.
  • Apaydin N, Kemiksiz E, Akcay A (2019) Comparison of manuka honey (Manuka Nd, G) and etacridine lactate (Rivanol) applications in the treatment of ınfected wounds in cats. Acta Scientiae Veterinariae 47: 1-7.
  • Ardıçlı S, Ustüner H, Arslan Ö, Kandazoğlu O (2019) Variability of CAPN1 g. 5709 C> G and MYF5 g. 1911 A> G polymorphisms in beef cattle imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 59(2): 72-78.
  • Arslan K, Akyüz B, Agaoglu OK (2015) Investigation of STAT5A, FSHR, and LHR gene polymorphisms in Turkish indigenous cattle breeds (East Anatolian Red, South Anatolian Red, Turkish Grey, Anatolian Black, and Zavot). Russian Journal of Genetics 51(11): 1088-1095.
  • Bao B, Zhang C, Fang X (2010) Association between polymorphism in STAT5A gene and milk production traits in Chinese Holstein cattle. Animal Science Papers and Reports 28(1): 5-11.
  • Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar MU (2019). DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25(1): 9-15.
  • Brym P, Kamiñski S, Rusc A (2004) New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. Journal of Applied Genetics 45(4): 445-452.
  • Coşier V, Croitoriu V (2012) Research concerning the polymorphic expression of Pit-1 and STAT5A genes in cattle. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 69(1-2): 70-79.
  • Dario C, Dario M, Ciotola F, Peretti V, Carnicella D, Selvaggi M (2009a) Analysis of STAT5A/AvaI gene polymorphism in four Italian cattle breeds. Biochem Genetics 47(9-10): 671-679.
  • Dario C, Selvaggi M, Carnicella D, Bufano G (2009b) STAT5A/AvaI polymorphism in Podolica bulls and its effect on growth performance traits. Livestock Science 123(1): 83-87.
  • Dario C, Selvaggi M (2011) Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits. Molecular Biology Reports 38: 5387-5392.
  • Flisikowski K, Oprządek J, Dymnicki E, Zwierzchowski L (2003) New polymorphism in the bovine STAT5A gene and its association with meat production traits in beef cattle. Animal Science Papers and Reports 21(3): 147-157.
  • He X, Chu MX, Qiao L, He JN, Wang PQ, Feng T, Di R, Cao GL, Fang L, An YF (2012) Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows. Molecular Biology Reports 39(3): 2901-2907.
  • Işık R, Bilgen G, Koşum N, Kandemir Ç, Taşkın T (2017) Polymorphism in exon 7 of β-lactoglobulin (β-LG) gene and its association with milk yield in Saanen goats. Tekirdağ Ziraat Fakültesi Dergisi, The Special Issue of Second International Balkan Agriculture Congress, pp. 35-40.
  • Karslı T, Balcıoğlu MS, Demir E, Fidan HG, Aslan M, Aktan S, Kamnlı S, Karabağ K, Şahin E (2017) Ankara Tavukçuluk Araştırma Enstitüsü’nde yetiştirilen yumurtacı saf tavuk hatlarında yumurta verimi ile ilişkili IGF-I ve NPY aday genlerindeki polimorfizmlerin belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology 5(9): 1051-1056.
  • Kıyıcı JM, Arslan K, Akyuz B, Kaliber M, Aksel EG. Çinar MU (2018) Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. International Journal of Dairy Technology 72(1): 1-7.
  • Kim AR, Kim K M, Byun MR, Hwang JH, Park JI, Oh HT, Kim HK, Jeong MG, Hwang ES, Hong JH (2017) Catechins activate muscle stem cells by MYF5 induction and stimulate muscle regeneration. Biochemical and Biophysical Research Communications 489(2): 142-148.
  • Kmieć M, Kowalewska-Łuczak I, Wojdak-Maksymiec K, Kulig H, Grzelak T (2010) STAT5A/AvaI restriction polymorphism in cows of Polish Red-and-White variety of Holstein Friesian breed. Russian Journal of Genetics 46(1): 81-85.
  • Lee C, Pollak EJ (2002) Genetic antagonism between body weight and milk production in beef cattle. Journal of Animal Science 80(2): 316-321.
  • Li C, Basarab J, Snelling WM, Benkel B, Murdoch B, Hansen C, Moore SS (2004) Assessment of positional candidate genes MYF5 and igf1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. Journal of Animal Science 82: 1-7.
  • Oikonomou G, Michailidis G, Kougioumtzis A, Avdi M, Banos G (2011) Effect of polymorphisms at the STAT5A and FGF2 gene loci on reproduction, milk yield and lameness of Holstein cows. Research in Veterinary Science 91(2): 235-239.
  • Sadeghi M, Shahrbabak MM, Mianji GR, Javaremi AN (2009) Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls. Livestock Science 123(1): 97-100.
  • Sambrook J, Frisch FF, Maniation T (1989) Spectrophotometric Determination of the Amount of DNA or RNA Appendix E: Commonly Used Techniques in Molecular Cloning. Molecular Cloning: 3A Laboratory Manual, Second Edition. Cold Spring Harbor Laboratory Press, New York.
  • Selvaggi M, Dario C, Normanno G, Celano GV, Dario M (2009) Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. Journal of Dairy Research 76(4): 441-445.
  • Selvaggi M, Tufarelli V, Pinto F, Centoducati G, Dambrosio A, Santacroce MP, Dario C (2013) Bovine STAT5A gene polymorphism analysis and its association with milk composition traits in Jersey cows. International Journal of Bioscience, Biochemistry and Bioinformatics 3(4): 341-344.
  • Selvaggi M, Albarella S, Dario C, Peretti V, Ciotola F (2017) Association of STAT5A gene variants with milk production traits in Agerolese cattle. Biochem Genetics 55(2): 158-167.
  • Selvaggi M, D’Alessandro AG, Dario C (2016) Bovine STAT5A gene polymorphism and its influence on growth traits in Podolica breed. Animal Production Science 56(7): 1056-1060.
  • Şahin Ş, Öner Y, Elmacı C (2013) Esmer ve Siyah Alaca ırkı sığırlarda bazı ekonomik özellikler ile ilişkili gen bölgelerinin PCR-RFLP tekniği ile incelenmesi. Tarım Bilimleri Dergisi 19: 235-244.
  • Şahin, Akyüz B (2017) Türkiye’de yetiştirilen beş sığır ırkında MYF5 gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi. Mediterranean Agricultural Sciences 30(1): 35-38.
  • Thornton PK (2010) Livestock production: Recent trends, future prospects. Philosophical Transactions of the Royal Society B: Biological Sciences 365: 2853-2867.
  • Wiggans GR, Cole JB, Hubbard SM, Sonstegard TS (2017) Genomic selection in dairy cattle: the USDA experience. Annual Review of Animal Biosciences 5: 309-327.

Investigation of the relationship between STAT5A and MYF5 gene polymorphisms and milk yield in simental cattle breed

Year 2021, Volume: 34 Issue: 1, 161 - 167, 01.04.2021
https://doi.org/10.29136/mediterranean.796773

Abstract

In this study, it is aimed to investigate the relationship between STAT5A-AvaI and MYF5-TaqI polymorphisms with daily and total lactation milk yields in a total of 202 animals belonging to Simmental cattle breed. Amplificated by PCR technique, 215 bp length of STAT5A gene region was digested with AvaI restriction enzyme, while 512 bp length of MYF5 gene region was digested with TaqIenzyme. The relationship between the determined STAT5A-AvaI and MYF5-TaqI genotypes and milk yields was evaluated with One-Way Variance Analysis (ANOVA). It was determined that the examined Simmental cattle population was in Hardy-Weinberg (H-W) equilibrium in terms of STAT5A-AvaI and MYF5-TaqI polymorphisms. It was observed that cattle with CT genotype had higher milk yield in both first and second lactations than other genotypes for STAT5A-AvaI polymorphism. On the other hand, it was determined that there was no relationship between MYF5-TaqI polymorphism and milk yield. As a result, it is thought that the STAT5A-AvaI polymorphism could be used to improve daily and total lactation milk yields in Simmental cattle breed.

Project Number

TYL-2019-8802

References

  • Ağaoğlu ÖK, Akyüz B, Zeytünlü E, Ağaoğlu AR (2020) Investigation of G+ 265C and G-1539A single nucleotide polymorphisms of toll-like receptor 4 gene (TLR4) in some cattle breeds raised in Turkey. SlovenianVeterinary Research 57(1): 25-31.
  • Apaydin N, Kemiksiz E, Akcay A (2019) Comparison of manuka honey (Manuka Nd, G) and etacridine lactate (Rivanol) applications in the treatment of ınfected wounds in cats. Acta Scientiae Veterinariae 47: 1-7.
  • Ardıçlı S, Ustüner H, Arslan Ö, Kandazoğlu O (2019) Variability of CAPN1 g. 5709 C> G and MYF5 g. 1911 A> G polymorphisms in beef cattle imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 59(2): 72-78.
  • Arslan K, Akyüz B, Agaoglu OK (2015) Investigation of STAT5A, FSHR, and LHR gene polymorphisms in Turkish indigenous cattle breeds (East Anatolian Red, South Anatolian Red, Turkish Grey, Anatolian Black, and Zavot). Russian Journal of Genetics 51(11): 1088-1095.
  • Bao B, Zhang C, Fang X (2010) Association between polymorphism in STAT5A gene and milk production traits in Chinese Holstein cattle. Animal Science Papers and Reports 28(1): 5-11.
  • Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar MU (2019). DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25(1): 9-15.
  • Brym P, Kamiñski S, Rusc A (2004) New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. Journal of Applied Genetics 45(4): 445-452.
  • Coşier V, Croitoriu V (2012) Research concerning the polymorphic expression of Pit-1 and STAT5A genes in cattle. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 69(1-2): 70-79.
  • Dario C, Dario M, Ciotola F, Peretti V, Carnicella D, Selvaggi M (2009a) Analysis of STAT5A/AvaI gene polymorphism in four Italian cattle breeds. Biochem Genetics 47(9-10): 671-679.
  • Dario C, Selvaggi M, Carnicella D, Bufano G (2009b) STAT5A/AvaI polymorphism in Podolica bulls and its effect on growth performance traits. Livestock Science 123(1): 83-87.
  • Dario C, Selvaggi M (2011) Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits. Molecular Biology Reports 38: 5387-5392.
  • Flisikowski K, Oprządek J, Dymnicki E, Zwierzchowski L (2003) New polymorphism in the bovine STAT5A gene and its association with meat production traits in beef cattle. Animal Science Papers and Reports 21(3): 147-157.
  • He X, Chu MX, Qiao L, He JN, Wang PQ, Feng T, Di R, Cao GL, Fang L, An YF (2012) Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows. Molecular Biology Reports 39(3): 2901-2907.
  • Işık R, Bilgen G, Koşum N, Kandemir Ç, Taşkın T (2017) Polymorphism in exon 7 of β-lactoglobulin (β-LG) gene and its association with milk yield in Saanen goats. Tekirdağ Ziraat Fakültesi Dergisi, The Special Issue of Second International Balkan Agriculture Congress, pp. 35-40.
  • Karslı T, Balcıoğlu MS, Demir E, Fidan HG, Aslan M, Aktan S, Kamnlı S, Karabağ K, Şahin E (2017) Ankara Tavukçuluk Araştırma Enstitüsü’nde yetiştirilen yumurtacı saf tavuk hatlarında yumurta verimi ile ilişkili IGF-I ve NPY aday genlerindeki polimorfizmlerin belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology 5(9): 1051-1056.
  • Kıyıcı JM, Arslan K, Akyuz B, Kaliber M, Aksel EG. Çinar MU (2018) Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. International Journal of Dairy Technology 72(1): 1-7.
  • Kim AR, Kim K M, Byun MR, Hwang JH, Park JI, Oh HT, Kim HK, Jeong MG, Hwang ES, Hong JH (2017) Catechins activate muscle stem cells by MYF5 induction and stimulate muscle regeneration. Biochemical and Biophysical Research Communications 489(2): 142-148.
  • Kmieć M, Kowalewska-Łuczak I, Wojdak-Maksymiec K, Kulig H, Grzelak T (2010) STAT5A/AvaI restriction polymorphism in cows of Polish Red-and-White variety of Holstein Friesian breed. Russian Journal of Genetics 46(1): 81-85.
  • Lee C, Pollak EJ (2002) Genetic antagonism between body weight and milk production in beef cattle. Journal of Animal Science 80(2): 316-321.
  • Li C, Basarab J, Snelling WM, Benkel B, Murdoch B, Hansen C, Moore SS (2004) Assessment of positional candidate genes MYF5 and igf1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. Journal of Animal Science 82: 1-7.
  • Oikonomou G, Michailidis G, Kougioumtzis A, Avdi M, Banos G (2011) Effect of polymorphisms at the STAT5A and FGF2 gene loci on reproduction, milk yield and lameness of Holstein cows. Research in Veterinary Science 91(2): 235-239.
  • Sadeghi M, Shahrbabak MM, Mianji GR, Javaremi AN (2009) Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls. Livestock Science 123(1): 97-100.
  • Sambrook J, Frisch FF, Maniation T (1989) Spectrophotometric Determination of the Amount of DNA or RNA Appendix E: Commonly Used Techniques in Molecular Cloning. Molecular Cloning: 3A Laboratory Manual, Second Edition. Cold Spring Harbor Laboratory Press, New York.
  • Selvaggi M, Dario C, Normanno G, Celano GV, Dario M (2009) Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. Journal of Dairy Research 76(4): 441-445.
  • Selvaggi M, Tufarelli V, Pinto F, Centoducati G, Dambrosio A, Santacroce MP, Dario C (2013) Bovine STAT5A gene polymorphism analysis and its association with milk composition traits in Jersey cows. International Journal of Bioscience, Biochemistry and Bioinformatics 3(4): 341-344.
  • Selvaggi M, Albarella S, Dario C, Peretti V, Ciotola F (2017) Association of STAT5A gene variants with milk production traits in Agerolese cattle. Biochem Genetics 55(2): 158-167.
  • Selvaggi M, D’Alessandro AG, Dario C (2016) Bovine STAT5A gene polymorphism and its influence on growth traits in Podolica breed. Animal Production Science 56(7): 1056-1060.
  • Şahin Ş, Öner Y, Elmacı C (2013) Esmer ve Siyah Alaca ırkı sığırlarda bazı ekonomik özellikler ile ilişkili gen bölgelerinin PCR-RFLP tekniği ile incelenmesi. Tarım Bilimleri Dergisi 19: 235-244.
  • Şahin, Akyüz B (2017) Türkiye’de yetiştirilen beş sığır ırkında MYF5 gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi. Mediterranean Agricultural Sciences 30(1): 35-38.
  • Thornton PK (2010) Livestock production: Recent trends, future prospects. Philosophical Transactions of the Royal Society B: Biological Sciences 365: 2853-2867.
  • Wiggans GR, Cole JB, Hubbard SM, Sonstegard TS (2017) Genomic selection in dairy cattle: the USDA experience. Annual Review of Animal Biosciences 5: 309-327.
There are 31 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering
Journal Section Makaleler
Authors

Osman Tufan Ertan 0000-0002-1821-8388

Bilal Akyüz 0000-0001-7548-9830

Project Number TYL-2019-8802
Publication Date April 1, 2021
Submission Date September 18, 2020
Published in Issue Year 2021 Volume: 34 Issue: 1

Cite

APA Ertan, O. T., & Akyüz, B. (2021). Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması. Mediterranean Agricultural Sciences, 34(1), 161-167. https://doi.org/10.29136/mediterranean.796773
AMA Ertan OT, Akyüz B. Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması. Mediterranean Agricultural Sciences. April 2021;34(1):161-167. doi:10.29136/mediterranean.796773
Chicago Ertan, Osman Tufan, and Bilal Akyüz. “Simental ırkı sığırlarda STAT5A Ve MYF5 Gen Polimorfizmleri Ile süt Verimi arasındaki ilişkinin araştırılması”. Mediterranean Agricultural Sciences 34, no. 1 (April 2021): 161-67. https://doi.org/10.29136/mediterranean.796773.
EndNote Ertan OT, Akyüz B (April 1, 2021) Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması. Mediterranean Agricultural Sciences 34 1 161–167.
IEEE O. T. Ertan and B. Akyüz, “Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması”, Mediterranean Agricultural Sciences, vol. 34, no. 1, pp. 161–167, 2021, doi: 10.29136/mediterranean.796773.
ISNAD Ertan, Osman Tufan - Akyüz, Bilal. “Simental ırkı sığırlarda STAT5A Ve MYF5 Gen Polimorfizmleri Ile süt Verimi arasındaki ilişkinin araştırılması”. Mediterranean Agricultural Sciences 34/1 (April 2021), 161-167. https://doi.org/10.29136/mediterranean.796773.
JAMA Ertan OT, Akyüz B. Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması. Mediterranean Agricultural Sciences. 2021;34:161–167.
MLA Ertan, Osman Tufan and Bilal Akyüz. “Simental ırkı sığırlarda STAT5A Ve MYF5 Gen Polimorfizmleri Ile süt Verimi arasındaki ilişkinin araştırılması”. Mediterranean Agricultural Sciences, vol. 34, no. 1, 2021, pp. 161-7, doi:10.29136/mediterranean.796773.
Vancouver Ertan OT, Akyüz B. Simental ırkı sığırlarda STAT5A ve MYF5 gen polimorfizmleri ile süt verimi arasındaki ilişkinin araştırılması. Mediterranean Agricultural Sciences. 2021;34(1):161-7.

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