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Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri

Year 2018, Volume: 33 Issue: 2, 116 - 123, 22.06.2018
https://doi.org/10.7161/omuanajas.387380

Abstract

BBu çalışmada, Metarhizium anisopliae (Metch) Sorok ve Beauveria bassiana (Bals.) Vuill. izolatları ve ticari
preparatlarının (Bio-Magic ve Bio-Power) laboratuvar koşullarında
Curculio nucum L.’a karşı etkinliği
değerlendirilmiştir. Bu izolatlar ve ticari preparatların 1×108 spor mL-1
konsantrasyonları Potter ilaçlama kulesi kullanılarak petri içerisinde bulunan
C. nucum erginleri üzerine
uygulanmıştır. Kontrol petri kaplarına ise % 0.02 Tween 20 içeren steril saf su
püskürtülmüştür. Petri kapları 25±1°C ve % 70±5 nemli ortamda 10 gün boyunca inkübe
edilmiştir. Ölü bireyler uygulamadan sonra 10 gün boyunca günlük olarak
sayılmıştır. Her bir gözlem günü için 4 tekerrür ve her petride 5 böcek olmak
üzere farklı böcek grupları kullanılmış (n=20 ergin böcek/gün/izolat veya
preparat) ve böylece zaman içindeki gözlemlerin ölüm üzerindeki bağımsızlığı
temin edilmiştir. Entomopatojenik funguslar için LT50 ve LT90 değerleri Probit
analizi ile hesaplanmıştır. Sonuç olarak,
M.
anisopliae
TR-106 izolatının LT50 ve LT90 değerleri sırasıyla 5.32 ve 7.85
gün iken, bu değerler Bio-Magic preparatı için sırasıyla 5.46 ve 10.16 gün
olarak belirlenmiştir.
B. bassiana
TR-217 izolatı için aynı değerler sırasıyla 4.64 ve 8.53 gün olarak
bulunmuştur. Son olarak, Bio-Power preparatının LT50 ve LT90 değerleri
sırasıyla 6.16 ve 9.86 gün olarak tespit edilmiştir. Ayrıca, bu izolatlar ve
preparatlar 10 gün sonra C. nucum erginlerinde % 100 ölüme neden olmuştur. Bu
sonuçlar,
M. anisopliae ve B. bassiana'nın fındık kurduna karşı
mücadelede umut verici biyolojik mücadele etmenleri olabileceğini
göstermektedir.

References

  • Prazak, R.A., 1991. Studies on indirect infection of Trypodendron lineatum Oliv with Beauveria bassiana (Bals.) Vuill. Zeitzchrift fur Angewandte Entomologie, 111: 431–441.
  • Ecevit, O., Tuncer, C., Özman S., Mennan, S., Akça, İ., 1996. Karadeniz Bölgesi fındık bahçelerindeki doğal düşmanlar ve biyolojik savaşımda kullanılma olanakları. Fındık ve diğer sert kabuklu meyveler sempozyumu, Ondokuz Mayıs Üniversitesi Ziraat Fakültesi, 295-296 s, Samsun.
  • Prazak, R.A., 1997. Laboratory evaluation of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hypomycetes) against Trypodendron lineatum Oliv. (Coleoptera: Scolytidae). Journal of Plant Diseases and Protection, 104: 459–465.
  • Pucci, C., 1992. Studies on population dynamics of Balaninus nucum L. (Col.: Cuculionidae) noxious to the hazel (Corylus avellana L.) in Northern Latium (Central Italy). J.of Appl. Ent., 114 (1): 5-16.
  • Akça, İ., Tuncer, C., 2005. Biological control and morphological studies on nut weevil (Curculio nucum L. Col., Curculıonıdae). Acta Hort. 686 ISHS 413-420.
  • Robertson, J. L., Russell, R. M., Preisler, H. K., Savin, E., 2007. Bioassays with Arthropods. Second edition. CRC Press, 199p.
  • Roy, H. E., Steinkraus, D. C., Eilenberg J., Hajek A. E., Pell, J. K., 2006. Bizarre interactions and endgames: Entomopathogenic fungi and their arthropod hosts. Annu. Rev. Entomol., 51: 331– 57.
  • Saruhan, I., Erper, I., Tuncer, C., Akca, I., 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera: Aphididae). Pak. J. Agri. Sci., 52(2): 273–278.
  • Saruhan, İ., Tuncer, C., 2001. Population densities and seasonal fluctiations of hazelnut pests in Samsun, Turkey. Proc. V. Int. Congress on Hazelnut. In: S.A. Mehlenbacher (eds). Acta Hort., 556. ISHS 495-502.
  • Scholte, E.J., Knols, B.J., Takken, W., 2005. A study on avoidance and repellency of the African malaria vector Anopheles gambiae upon exposure to the entomopathogenic fungus Metarhizium anisopliae. Pest Control, 16: 131–138.
  • Kocacevik, S., Sevim, A., Eroglu, M., Demirbag, Z., Demir, I., 2015. Molecular characterization, virulence and horizontal transmission of Beauveria pseudobassiana from Dendroctonus micans (Kug.) (Coleoptera: Curculionidae). J. Appl. Entomol., 139: 381–389.
  • Sevim, A., Demir, I., Höfte, M., Humber, R.A., Demirbag, Z., (2010). Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey. Biocontrol, 59: 279–297.
  • Tuncer, C., Ecevit, O., 1997. Current status of hazelnut pests in Turkey. Acta Hort., 445. ISHS 1997, 545-550.
  • Erper I., Saruhan, I., Akca, I., Aksoy, H.M., Tuncer, C., 2016. Evaluation of some entomopathogenic fungi for controlling the Green Shield Bug, Palomena prasina L. (Heteroptera: Pentatomidae). Egyptian Journal of Biological Pest Control, 26(3): 573–578.
  • Tuncer, C., Knížek, M., Hulcr. J., (2017). Scolytinae in hazelnut orchards of Turkey: clarification of species and identification key (Coleoptera, Curculionidae). ZooKeys, 710: 65–76.
  • Tuncer, T., Kushiyev, R., Saruhan, I., Erper, I., 2016. Entomopatojen funguslar Metarhizium anisopliae ve Baeuveria bassiana’nın Xylosandrus germanus (Coleoptera: Curculionidae: Scolytinae)’a karşı etkinliğinin belirlenmesi. Türkiye VI. Bitki Koruma Kongresi, 126 s, 5-8 Eylül, Konya.
  • Villani, M.G., Krueger, S.R., Schroeder, P.C., Consolie, F., Consolie, N.H., Prestonwilsey, L.M., Roberts, D.W., 1994. Soil application effects of Metarhizium anisopliae on Japanese beetle (Coleoptera: Scarabeidae) behavior and survival in turf grass microcosms. Environ. Entomol., 23: 502–513.
  • AliNiazee, M.T., 1998. Ecology and management of hazelnut pests. Annu. Rev. Entomol., 43: 395-419.
  • Zimmermann, G., 2007a. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocont. Sci. Technol., 17: 553–596.
  • Zimmermann, G., 2007b. Review on Safety of the Entomopathogenic Fungus Metarhizium anisopliae. Biocontrol Science and Technology, 17: 879-920.
  • Zimmermann, G., 2008. The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont. Sci. Technol., 18: 865–901.
  • Akça, İ., 2003. Orta Karadeniz Bölgesinde fındık kurdu Curculio nucum L. (Coleoptera; Curculionidae) populasyonlarının biyolojisi ve zararı üzerine araştırmalar. Doktora Tezi. Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, Samsun.
  • Ansari, M.A., Butt, T.M., 2012. Susceptibility of different developmental stages of large pine weevil Hylobius abietis (Coleoptera: Curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods. Journal of Invertebrate Pathology, 111 (1): 33-40.
  • Carrillo, D., Dunlap, C. A., Avery, P. B., Navarrete, J., Duncan, R. E., Jackson, M. A., Behle, R. W., Cave, R. D., Crane, J., Rooney, A. P., Peña, J. E., 2015. Entomopathogenic fungi as biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae). Biol. Control, 81:44-50.
  • Castrillo, L.A., Griggs, M.H., Ranger, C.M., Reding, M.E., Vandenberg, J.D., 2011. Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Curculionidae) and impact on brood. Biological Control, 58:121–126.
  • Gindin, G., Levinski, S., Glazer, I., Soroker, V., 2006. Evaluation of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against the red palm weevil. Phytoparasitica, 34(4):370-379.
  • Castrillo, L.A., Griggs, M.H., Vandenberg, J.D., 2013. Granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae), survival and brood production following exposure to entomopathogenic and mycoparasitic fungi. Biol. Control, 67: 220–226.
  • Ceyhan, V., Bozoğlu, M., Cinemre, H.A., 2002. Bafra ve Çarşamba ovalarında kimyasal girdi kullanım düzeyi ve çevreye etkileri. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 17 (2): 17-23.
  • Cheng, Y., Liu T., Zhao, Y., Geng, W., Chen, L., Liu, J., 2016. Evaluation of pathogenicity of the fungi Metarhizium anisopliae and Beauveria bassiana in Hazelnut weevil (Curculio nucum L., Coleoptera, Curculionidae) larvae. Indian J Microbiol, 56(4): 405–410
  • Glare, T.R., Placet, C., Nelson, T.L., Reay, S.D., 2002. Potential of Beauveria and Metarhizium as control agents of pinhole borers (Platypus spp.), 73-79.
  • Goettel, M.S., Eilenberg, J., Glare, T., 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert, L.I., Iatrou, K. Gill, S.S., (eds). Comprehensive Molecular Insect Science, 361- 405 p, Amsterdam.
  • Hirsch, J., Reineke, A., 2014. Efficiency of commercial entomopathogenic fungal species against different members of the genus Otiorhynchus (Coleoptera: Curculionidae) under laboratory and semi-field conditions. Journal of Plant Diseases and Protection, 121 (5): 211–218.
  • Kreutz, J., Vaupel, O., Zimmermann, G., 2004. Horizontal transmission of the entomopathogenic fungus Beauveria bassiana among the Spruce bark beetle, Ips typographus (Col., Scolytidae) in the laboratory and under field conditions. Biocont. Sci. Technol., 14: 837–848.
  • Ihara, F., Toyama, M., Sato, T., 2003. Pathogenicity of Metarhizium anisopliae to the chestnut weevil larvae under laboratory and field conditions. Appl. Entomol. Zool., 38 (4): 461–465.
  • Ioachim, E., Bobarnac, B., 1997. Research on the hazelnut pests in Romania. Acta Hort., 445. ISHS 527-537.
  • Işık, M., Ecevit, O., Kurt, M.A., Yücetin, T., 1987. Doğu Karadeniz Bölgesi fındık bahçelerinde entegre savaş olanakları üzerinde araştırmalar. Ondokuz Mayıs Üniversitesi Yayınları, No: 20,95s.
  • Kushiyev, R., 2015. Fındıkta önemli yazıcı böcek türlerindeki funguslarin belirlenmesi. Yüksek lisans tezi, Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, 63 s, Samsun.
  • Kushiyev, R., Tuncer, C., Erper, I., Saruhan. I., 2017. Effectiveness of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against Anisandrus dispar (Coleoptera: Curculıonıdae: Scolytınae). Ⅸ. International congress on Hazelnut, 152 p, 15-19 August, Atakum-Samsun/ Turkiye.
  • Liu, J., Zhang, Y., Liu, T., Tuncer, C., Cheng, Y., 2017. Screening of a highly pathogenic strain against hazelnut weevil and microscopic observation on its infection process. Journal of Beijing Forestry University, 39( 3): 32-37.
  • Milenkovic, S., Mitrovic, M., 2001. Hazelnut pests in Serbia. Proc. V. Int. Congress on Hazelnut. In: S.A. Mehlenbacher (eds). Acta Hort., 556. ISHS 403-409.
  • Milner, R.J., Staples, J.A., 1996. Biological control of termites: results and experiences within a CSIRO project in Australia. Biocontrol Sci. Tech., 6: 3–9.
  • Paparatti, B., Speranza, S., 2005. Biological Control of Hazelnut weevil (Curculio nucum L., Coleoptera, Curculionidae) using the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuill. (Deuteromycotina, Hyphomycetes). Proc. VI.th Intl. Congress on Hazelnut, Acta Hort., 686

Efficacies of Metarhizium anisopliae and Beauvaria bassiana isolates and products against Curculio nucum

Year 2018, Volume: 33 Issue: 2, 116 - 123, 22.06.2018
https://doi.org/10.7161/omuanajas.387380

Abstract

In this study, the efficacies of Metarhizium anisopliae (Metch) Sorok and
Beauveria bassiana (Bals.) Vuill.
isolates and commercial products (Bio-Magic and Bio-Power) were tested against Curculio nucum L. under laboratory
conditions. Conidial suspensions of these isolates and products at
concentration of 1×108 conidia mL-1 were applied on the adults of C. nucum in petri dishes, using a Potter
spray tower. Control insects were sprayed only with sterile-distilled-water
containing 0.02 % Tween 20. All treated insects were incubated at 25±1°C and
70±5 % RH photoperiod in an incubator for 10 days.  Dead individuals were counted daily following
treatment for 10 days. Different groups of insects were used for each
observation days, with 4 replications and 5 insects per petri dish (n=20
insect/day/isolate or preparation), and thus independence of observations on
the mortality over time was ensured. Lethal time values (LT50 and LT90) for
entomopathogenic fungi were calculated using Probit analysis. As a result, LT50
and LT90 values of M. anisopliae
TR-106 isolate were 5.32 and 7.85 day, respectively while these values for
Bio-Magic product were determined as 5.46 and 10.16 days, respectively.  LT50 and LT90 for B. bassiana TR-217 isolate were found to be 4.64 and 8.53 day,
respectively. Finally, LT50 and LT90 values of Bio-Power product were
determined as 6.16 and 9.86 day, respectively. In addition, these isolates and
products have caused 100 % mortality on C. nucum adults within 10 days. The
results indicated that M. anisopliae
and B. bassiana could be promising
biological agents to control hazelnut weevil.

References

  • Prazak, R.A., 1991. Studies on indirect infection of Trypodendron lineatum Oliv with Beauveria bassiana (Bals.) Vuill. Zeitzchrift fur Angewandte Entomologie, 111: 431–441.
  • Ecevit, O., Tuncer, C., Özman S., Mennan, S., Akça, İ., 1996. Karadeniz Bölgesi fındık bahçelerindeki doğal düşmanlar ve biyolojik savaşımda kullanılma olanakları. Fındık ve diğer sert kabuklu meyveler sempozyumu, Ondokuz Mayıs Üniversitesi Ziraat Fakültesi, 295-296 s, Samsun.
  • Prazak, R.A., 1997. Laboratory evaluation of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hypomycetes) against Trypodendron lineatum Oliv. (Coleoptera: Scolytidae). Journal of Plant Diseases and Protection, 104: 459–465.
  • Pucci, C., 1992. Studies on population dynamics of Balaninus nucum L. (Col.: Cuculionidae) noxious to the hazel (Corylus avellana L.) in Northern Latium (Central Italy). J.of Appl. Ent., 114 (1): 5-16.
  • Akça, İ., Tuncer, C., 2005. Biological control and morphological studies on nut weevil (Curculio nucum L. Col., Curculıonıdae). Acta Hort. 686 ISHS 413-420.
  • Robertson, J. L., Russell, R. M., Preisler, H. K., Savin, E., 2007. Bioassays with Arthropods. Second edition. CRC Press, 199p.
  • Roy, H. E., Steinkraus, D. C., Eilenberg J., Hajek A. E., Pell, J. K., 2006. Bizarre interactions and endgames: Entomopathogenic fungi and their arthropod hosts. Annu. Rev. Entomol., 51: 331– 57.
  • Saruhan, I., Erper, I., Tuncer, C., Akca, I., 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera: Aphididae). Pak. J. Agri. Sci., 52(2): 273–278.
  • Saruhan, İ., Tuncer, C., 2001. Population densities and seasonal fluctiations of hazelnut pests in Samsun, Turkey. Proc. V. Int. Congress on Hazelnut. In: S.A. Mehlenbacher (eds). Acta Hort., 556. ISHS 495-502.
  • Scholte, E.J., Knols, B.J., Takken, W., 2005. A study on avoidance and repellency of the African malaria vector Anopheles gambiae upon exposure to the entomopathogenic fungus Metarhizium anisopliae. Pest Control, 16: 131–138.
  • Kocacevik, S., Sevim, A., Eroglu, M., Demirbag, Z., Demir, I., 2015. Molecular characterization, virulence and horizontal transmission of Beauveria pseudobassiana from Dendroctonus micans (Kug.) (Coleoptera: Curculionidae). J. Appl. Entomol., 139: 381–389.
  • Sevim, A., Demir, I., Höfte, M., Humber, R.A., Demirbag, Z., (2010). Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey. Biocontrol, 59: 279–297.
  • Tuncer, C., Ecevit, O., 1997. Current status of hazelnut pests in Turkey. Acta Hort., 445. ISHS 1997, 545-550.
  • Erper I., Saruhan, I., Akca, I., Aksoy, H.M., Tuncer, C., 2016. Evaluation of some entomopathogenic fungi for controlling the Green Shield Bug, Palomena prasina L. (Heteroptera: Pentatomidae). Egyptian Journal of Biological Pest Control, 26(3): 573–578.
  • Tuncer, C., Knížek, M., Hulcr. J., (2017). Scolytinae in hazelnut orchards of Turkey: clarification of species and identification key (Coleoptera, Curculionidae). ZooKeys, 710: 65–76.
  • Tuncer, T., Kushiyev, R., Saruhan, I., Erper, I., 2016. Entomopatojen funguslar Metarhizium anisopliae ve Baeuveria bassiana’nın Xylosandrus germanus (Coleoptera: Curculionidae: Scolytinae)’a karşı etkinliğinin belirlenmesi. Türkiye VI. Bitki Koruma Kongresi, 126 s, 5-8 Eylül, Konya.
  • Villani, M.G., Krueger, S.R., Schroeder, P.C., Consolie, F., Consolie, N.H., Prestonwilsey, L.M., Roberts, D.W., 1994. Soil application effects of Metarhizium anisopliae on Japanese beetle (Coleoptera: Scarabeidae) behavior and survival in turf grass microcosms. Environ. Entomol., 23: 502–513.
  • AliNiazee, M.T., 1998. Ecology and management of hazelnut pests. Annu. Rev. Entomol., 43: 395-419.
  • Zimmermann, G., 2007a. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocont. Sci. Technol., 17: 553–596.
  • Zimmermann, G., 2007b. Review on Safety of the Entomopathogenic Fungus Metarhizium anisopliae. Biocontrol Science and Technology, 17: 879-920.
  • Zimmermann, G., 2008. The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont. Sci. Technol., 18: 865–901.
  • Akça, İ., 2003. Orta Karadeniz Bölgesinde fındık kurdu Curculio nucum L. (Coleoptera; Curculionidae) populasyonlarının biyolojisi ve zararı üzerine araştırmalar. Doktora Tezi. Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, Samsun.
  • Ansari, M.A., Butt, T.M., 2012. Susceptibility of different developmental stages of large pine weevil Hylobius abietis (Coleoptera: Curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods. Journal of Invertebrate Pathology, 111 (1): 33-40.
  • Carrillo, D., Dunlap, C. A., Avery, P. B., Navarrete, J., Duncan, R. E., Jackson, M. A., Behle, R. W., Cave, R. D., Crane, J., Rooney, A. P., Peña, J. E., 2015. Entomopathogenic fungi as biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae). Biol. Control, 81:44-50.
  • Castrillo, L.A., Griggs, M.H., Ranger, C.M., Reding, M.E., Vandenberg, J.D., 2011. Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Curculionidae) and impact on brood. Biological Control, 58:121–126.
  • Gindin, G., Levinski, S., Glazer, I., Soroker, V., 2006. Evaluation of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against the red palm weevil. Phytoparasitica, 34(4):370-379.
  • Castrillo, L.A., Griggs, M.H., Vandenberg, J.D., 2013. Granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae), survival and brood production following exposure to entomopathogenic and mycoparasitic fungi. Biol. Control, 67: 220–226.
  • Ceyhan, V., Bozoğlu, M., Cinemre, H.A., 2002. Bafra ve Çarşamba ovalarında kimyasal girdi kullanım düzeyi ve çevreye etkileri. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 17 (2): 17-23.
  • Cheng, Y., Liu T., Zhao, Y., Geng, W., Chen, L., Liu, J., 2016. Evaluation of pathogenicity of the fungi Metarhizium anisopliae and Beauveria bassiana in Hazelnut weevil (Curculio nucum L., Coleoptera, Curculionidae) larvae. Indian J Microbiol, 56(4): 405–410
  • Glare, T.R., Placet, C., Nelson, T.L., Reay, S.D., 2002. Potential of Beauveria and Metarhizium as control agents of pinhole borers (Platypus spp.), 73-79.
  • Goettel, M.S., Eilenberg, J., Glare, T., 2005. Entomopathogenic fungi and their role in regulation of insect populations. In: Gilbert, L.I., Iatrou, K. Gill, S.S., (eds). Comprehensive Molecular Insect Science, 361- 405 p, Amsterdam.
  • Hirsch, J., Reineke, A., 2014. Efficiency of commercial entomopathogenic fungal species against different members of the genus Otiorhynchus (Coleoptera: Curculionidae) under laboratory and semi-field conditions. Journal of Plant Diseases and Protection, 121 (5): 211–218.
  • Kreutz, J., Vaupel, O., Zimmermann, G., 2004. Horizontal transmission of the entomopathogenic fungus Beauveria bassiana among the Spruce bark beetle, Ips typographus (Col., Scolytidae) in the laboratory and under field conditions. Biocont. Sci. Technol., 14: 837–848.
  • Ihara, F., Toyama, M., Sato, T., 2003. Pathogenicity of Metarhizium anisopliae to the chestnut weevil larvae under laboratory and field conditions. Appl. Entomol. Zool., 38 (4): 461–465.
  • Ioachim, E., Bobarnac, B., 1997. Research on the hazelnut pests in Romania. Acta Hort., 445. ISHS 527-537.
  • Işık, M., Ecevit, O., Kurt, M.A., Yücetin, T., 1987. Doğu Karadeniz Bölgesi fındık bahçelerinde entegre savaş olanakları üzerinde araştırmalar. Ondokuz Mayıs Üniversitesi Yayınları, No: 20,95s.
  • Kushiyev, R., 2015. Fındıkta önemli yazıcı böcek türlerindeki funguslarin belirlenmesi. Yüksek lisans tezi, Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, 63 s, Samsun.
  • Kushiyev, R., Tuncer, C., Erper, I., Saruhan. I., 2017. Effectiveness of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against Anisandrus dispar (Coleoptera: Curculıonıdae: Scolytınae). Ⅸ. International congress on Hazelnut, 152 p, 15-19 August, Atakum-Samsun/ Turkiye.
  • Liu, J., Zhang, Y., Liu, T., Tuncer, C., Cheng, Y., 2017. Screening of a highly pathogenic strain against hazelnut weevil and microscopic observation on its infection process. Journal of Beijing Forestry University, 39( 3): 32-37.
  • Milenkovic, S., Mitrovic, M., 2001. Hazelnut pests in Serbia. Proc. V. Int. Congress on Hazelnut. In: S.A. Mehlenbacher (eds). Acta Hort., 556. ISHS 403-409.
  • Milner, R.J., Staples, J.A., 1996. Biological control of termites: results and experiences within a CSIRO project in Australia. Biocontrol Sci. Tech., 6: 3–9.
  • Paparatti, B., Speranza, S., 2005. Biological Control of Hazelnut weevil (Curculio nucum L., Coleoptera, Curculionidae) using the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuill. (Deuteromycotina, Hyphomycetes). Proc. VI.th Intl. Congress on Hazelnut, Acta Hort., 686
There are 42 citations in total.

Details

Primary Language Turkish
Journal Section Plant Protection
Authors

Celal Tuncer

Rahman Kushiyev This is me

Jianfeng Liu This is me

İzzet Akça

Publication Date June 22, 2018
Acceptance Date June 10, 2018
Published in Issue Year 2018 Volume: 33 Issue: 2

Cite

APA Tuncer, C., Kushiyev, R., Liu, J., Akça, İ. (2018). Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. Anadolu Tarım Bilimleri Dergisi, 33(2), 116-123. https://doi.org/10.7161/omuanajas.387380
AMA Tuncer C, Kushiyev R, Liu J, Akça İ. Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. ANAJAS. June 2018;33(2):116-123. doi:10.7161/omuanajas.387380
Chicago Tuncer, Celal, Rahman Kushiyev, Jianfeng Liu, and İzzet Akça. “Metarhizium Anisopliae Ve Beauveria Bassiana Izolat Ve preparatlarının Curculio nucum’a karşı Etkinlikleri”. Anadolu Tarım Bilimleri Dergisi 33, no. 2 (June 2018): 116-23. https://doi.org/10.7161/omuanajas.387380.
EndNote Tuncer C, Kushiyev R, Liu J, Akça İ (June 1, 2018) Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. Anadolu Tarım Bilimleri Dergisi 33 2 116–123.
IEEE C. Tuncer, R. Kushiyev, J. Liu, and İ. Akça, “Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri”, ANAJAS, vol. 33, no. 2, pp. 116–123, 2018, doi: 10.7161/omuanajas.387380.
ISNAD Tuncer, Celal et al. “Metarhizium Anisopliae Ve Beauveria Bassiana Izolat Ve preparatlarının Curculio nucum’a karşı Etkinlikleri”. Anadolu Tarım Bilimleri Dergisi 33/2 (June 2018), 116-123. https://doi.org/10.7161/omuanajas.387380.
JAMA Tuncer C, Kushiyev R, Liu J, Akça İ. Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. ANAJAS. 2018;33:116–123.
MLA Tuncer, Celal et al. “Metarhizium Anisopliae Ve Beauveria Bassiana Izolat Ve preparatlarının Curculio nucum’a karşı Etkinlikleri”. Anadolu Tarım Bilimleri Dergisi, vol. 33, no. 2, 2018, pp. 116-23, doi:10.7161/omuanajas.387380.
Vancouver Tuncer C, Kushiyev R, Liu J, Akça İ. Metarhizium anisopliae ve Beauveria bassiana izolat ve preparatlarının Curculio nucum’a karşı etkinlikleri. ANAJAS. 2018;33(2):116-23.
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