Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması
Abstract
Araştırma, Sinop ili Gerze ilçesinde
bulunan koruma altındaki saf Gerze tavuğu popülasyonu ve aynı ilde
yetiştiriciliği yapılan köy tavuğu popülasyonları arasındaki genetik ilişkinin
belirlenmesi amacıyla yürütülmüştür. Çalışmada yüksek düzeyde polimorfizm
gösteren 8 adet mikrosatellit lokusu kullanılmıştır. Çalışmanın hayvan
materyalini, Sinop ili Ayancık, Boyabat ve Gerze ilçelerinde bulunan 3 köy
tavuğu popülasyonuna ait 60 örnek ve Gerze ilçesinde koruma altında tutulan 20
adet saf Gerze tavuğu oluşturmuştur. Sinop Gerze Köy, Sinop Gerze (Hacıkadı),
Sinop Ayancık Köy ve Sinop Boyabat Köy popülasyonlarında beklenen
heterozigotluk (He) değerleri sırasıyla 0.820, 0.755, 0.795 ve 0.741
olarak hesaplanmıştır. Popülasyon ayrımı yapılmaksızın hesaplanan akrabalı
yetiştirme katsayısı (FIS) değerleri LEI0166, MCW0034 ve MCW0069
lokuslarında -0.131, -0.195, -0.137 ve ADL0278, LEI0234, MCW0080, MCW0104,
MCW0330 lokuslarında ise sırasıyla 0.178, 0.134, 0.766, 0.508 ve 0.433 olarak
bulunmuştur. Temel koordinatlar ve Structure analizi sonuçları saf Gerze
(Hacıkadı) popülasyonunun diğer köy popülasyonlarından genetik olarak farklı
olduğunu ortaya koymuştur. Araştırmada, halk elinde yetiştiriciliği yapılan
Gerze tavuklarının saf olmadığı, bu ırkın korunması için bölgede saf olarak
yetiştirilmesinin gerekli olduğu sonucuna varılmıştır.
References
- Abebe, A.S., Mikko, S., Johansson, A.M., 2015. Genetic diversity of five local swedish chicken breeds detected by microsatellite markers. Plos One, 10, 4: e0120580.
- Anderson, M., Willis, T.J., 2003. Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology, 84, 2: 511-525.
- Anmarkrud, J.A., Kleven, O., Bachmann, L., Lifjeld, J.T., 2008. Microsatellite evolution: Mutations, sequence variation and homoplasy in the hypervariable avian microsatellite locus HrU10. BMC Evolutionary Biology, 8: 138-147.
- Bruford, M.W., Wayne, R.K., 1993. Microsatellites and their application to population genetic studies. Current Opinion in Genetics and Development, 3: 939-943.
- Chatterjee, R.N., Bhattacharya, T.K., Dange, M., Dushyanth, K., Niranjan, M., Reddy, B.L.N., Rajkumar, U., 2014. Genetic heterogeneity among various indigenous and other chicken populations with microsatellite markers. Journal of Applied Animal Research, 43, 3: 266-271.
- Chen, G., Bao, W., Shu, J., Ji, C., Wang, M., Eding, H., Muchadeyi, F., Weigend, S., 2008. Assessment of Population Structure and Genetic Diversity of 15 Chinese Indigenous Chicken Breeds Using Microsatellite Markers. Asian-Australasian Journal of Animal Sciences, 21, 3: 331 - 339.
- Elmaci, N., Berry, R.S., 1999. Principal Coordinate Analysis on a Protein Model. Journal of Chemical Physics. 110, 21: 10606-10622.
- Fadhıl, M., Mercan, L., 2017. Molecular Characterization of Mx Gene Polymorphism in Gerze Chicken Breed and Pure Line Chicken Breed. Animal Research International, 13, 3: 2540-2543.
- FAO, 2007. The State of the World’s Animal Genetic Resources for Food and Agriculture.
- FAO, 2017. FAOSTAT, http://www.fao.org/faostat/en/#data/QL.
- Granevitze, Z., Hillel, J., Chen, G.H., Cuc, N.T.K., Feldman, M., Eding, H., Weigend, S., 2007. Genetic diversity within chicken populations from different continents and management histories. Animal Genetics, 38: 576-83.
- Hillel, J., Granevitze, Z., Twito, T., Ben-Avraham, D., Blum, S., Lavi, U., David, L., Feldman, M.W., Cheng, H., Weigend, S., 2007. Molecular markers for assessment of chicken biodiversity. World Poultry Science Journal, 63: 33-45.
- Mercan, L., Okumuş, A., 2015. Genetic diversity of village chickens in central black sea region and commercial chickens in Turkey by using microsatellite markers. Turkish Journal of Veterinary and Animal Sciences, 39: 134-140.
- Notter, D. R., 1999. The importance of genetic diversity in livestock populations of the future. Journal of Animal Science, 77: 61-69.
- Peakall, R., Smouse, P.E., 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537-2539.
- Pritchard, J. K., Stephens, M., Donnelly, P., 2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945-959.
- Rohlf, F.J., 2000. Numeric Taxonomy System Version 2.11. NTSYSpc, East Setauket, New York, USA.
- Romanov, M.N., Wezyk, S., Cywa-Benko, K., Sakhatsky, N.I., 1996. Poultry genetic resources in the countries of Eastern Europe history and current state. Poultry Avian Biology, 7: 1-29.
- Romanov, M.N., Weigend, S., 2001. Analysis of genetic relationships between various populations of domestic and jungle fowl using microsatellite markers. Poultry Science, 80: 1057-1063.
- Sarıca, M., Yamak, U.S., Boz, M.A., Uçar, A., 2014. Geriye Melezlemeyle Üretilen Etçi Genotiplerin Bir Ticari Etlik Piliç Genotipiyle Büyüme, Kesim ve Karkas Özellikleri Bakımından Karşılaştırılması. Tavukçuluk Araştırma Dergisi, 11, 1: 5-9.
- Suh, S., Sharma, A., Lee, S., Cho, C.Y., Kim, J.H., Choi, S.B., Kim, H., Seong, H.H., Yeon, S.H., Kim, D.H., Ko, G.Y., 2014. Genetic diversity and relationships of Korean chicken breeds based on 30 microsatellite markers. Asian-Australasian Journal of Animal Sciences, 27, 10: 1399-1405.
- Tadano, R., Nishibori, M., Imamura, Y., Matsuzaki, M., Kinoshita, K., Mizutani, M., Namikawa, T., Tsudzuki, M., 2007. High genetic divergence in miniature breeds of japanese native chickens compared to red junglefowl, as revealed by microsatellite analysis. Animal Genetics, 39: 71-78.
- Wilkinson, S., Wiener, P., Teverson, D., Haleyand, C.S., Hocking, P.M., 2011. Characterization of the genetic diversity, structure and admixture of British chicken breeds. Animal Genetics, 43: 552-563.
- Wright, S., 1951, The Genetical Structure of Populations. Annals of Eugenics, 15: 323-354.
- Wright, S., 1969. Evolution and Genetics of Populations: The Theory of Gene Frequencies. Univ. Chicago Press, Chicago.
Comparison of Gerze and village chicken populations in Sinop by eight polymorphic microsatellite loci
Abstract
In this study, we aimed to identify the genetic
relation between indigenous Gerze chicken population which is conserved in
Gerze district of Sinop province and village chicken populations which are
raised in Sinop. We used 8 highly polymorphic microsatellite loci for
examinations. Animal materials of this study are total of 60 samples from 3
village chicken populations raised in Ayancık, Boyabat and Gerze districts, and
20 samples from Gerze chicken from the conserved population in Gerze district
of Sinop province. Expected heterozygosity (He) values in Sinop
Gerze Village, Sinop Gerze (Hacıkadı), Sinop Ayancık Village and Sinop Boyabat
Village populations were calculated as 0.820, 0.755, 0.795 and 0.741,
respectively. FIS values were predicted as -0.131, -0.195, -0.137 in
LEI0166, MCW0034 ve MCW0069 loci and 0.178, 0.134, 0.766, 0.508, 0.433 in
ADL0278, LEI0234, MCW0080, MCW0104, MCW0330 loci respectively. Principal
coordinate and Structure analyses’ results showed that Gerze (Hacıkadın)
population is genetically different from the other village populations. In the
study, it was concluded that the chickens in the Gerze villages are not pure
breed. Thus, pure breed raising is necessary to conserve this breed in the
region.
References
- Abebe, A.S., Mikko, S., Johansson, A.M., 2015. Genetic diversity of five local swedish chicken breeds detected by microsatellite markers. Plos One, 10, 4: e0120580.
- Anderson, M., Willis, T.J., 2003. Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology, 84, 2: 511-525.
- Anmarkrud, J.A., Kleven, O., Bachmann, L., Lifjeld, J.T., 2008. Microsatellite evolution: Mutations, sequence variation and homoplasy in the hypervariable avian microsatellite locus HrU10. BMC Evolutionary Biology, 8: 138-147.
- Bruford, M.W., Wayne, R.K., 1993. Microsatellites and their application to population genetic studies. Current Opinion in Genetics and Development, 3: 939-943.
- Chatterjee, R.N., Bhattacharya, T.K., Dange, M., Dushyanth, K., Niranjan, M., Reddy, B.L.N., Rajkumar, U., 2014. Genetic heterogeneity among various indigenous and other chicken populations with microsatellite markers. Journal of Applied Animal Research, 43, 3: 266-271.
- Chen, G., Bao, W., Shu, J., Ji, C., Wang, M., Eding, H., Muchadeyi, F., Weigend, S., 2008. Assessment of Population Structure and Genetic Diversity of 15 Chinese Indigenous Chicken Breeds Using Microsatellite Markers. Asian-Australasian Journal of Animal Sciences, 21, 3: 331 - 339.
- Elmaci, N., Berry, R.S., 1999. Principal Coordinate Analysis on a Protein Model. Journal of Chemical Physics. 110, 21: 10606-10622.
- Fadhıl, M., Mercan, L., 2017. Molecular Characterization of Mx Gene Polymorphism in Gerze Chicken Breed and Pure Line Chicken Breed. Animal Research International, 13, 3: 2540-2543.
- FAO, 2007. The State of the World’s Animal Genetic Resources for Food and Agriculture.
- FAO, 2017. FAOSTAT, http://www.fao.org/faostat/en/#data/QL.
- Granevitze, Z., Hillel, J., Chen, G.H., Cuc, N.T.K., Feldman, M., Eding, H., Weigend, S., 2007. Genetic diversity within chicken populations from different continents and management histories. Animal Genetics, 38: 576-83.
- Hillel, J., Granevitze, Z., Twito, T., Ben-Avraham, D., Blum, S., Lavi, U., David, L., Feldman, M.W., Cheng, H., Weigend, S., 2007. Molecular markers for assessment of chicken biodiversity. World Poultry Science Journal, 63: 33-45.
- Mercan, L., Okumuş, A., 2015. Genetic diversity of village chickens in central black sea region and commercial chickens in Turkey by using microsatellite markers. Turkish Journal of Veterinary and Animal Sciences, 39: 134-140.
- Notter, D. R., 1999. The importance of genetic diversity in livestock populations of the future. Journal of Animal Science, 77: 61-69.
- Peakall, R., Smouse, P.E., 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537-2539.
- Pritchard, J. K., Stephens, M., Donnelly, P., 2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945-959.
- Rohlf, F.J., 2000. Numeric Taxonomy System Version 2.11. NTSYSpc, East Setauket, New York, USA.
- Romanov, M.N., Wezyk, S., Cywa-Benko, K., Sakhatsky, N.I., 1996. Poultry genetic resources in the countries of Eastern Europe history and current state. Poultry Avian Biology, 7: 1-29.
- Romanov, M.N., Weigend, S., 2001. Analysis of genetic relationships between various populations of domestic and jungle fowl using microsatellite markers. Poultry Science, 80: 1057-1063.
- Sarıca, M., Yamak, U.S., Boz, M.A., Uçar, A., 2014. Geriye Melezlemeyle Üretilen Etçi Genotiplerin Bir Ticari Etlik Piliç Genotipiyle Büyüme, Kesim ve Karkas Özellikleri Bakımından Karşılaştırılması. Tavukçuluk Araştırma Dergisi, 11, 1: 5-9.
- Suh, S., Sharma, A., Lee, S., Cho, C.Y., Kim, J.H., Choi, S.B., Kim, H., Seong, H.H., Yeon, S.H., Kim, D.H., Ko, G.Y., 2014. Genetic diversity and relationships of Korean chicken breeds based on 30 microsatellite markers. Asian-Australasian Journal of Animal Sciences, 27, 10: 1399-1405.
- Tadano, R., Nishibori, M., Imamura, Y., Matsuzaki, M., Kinoshita, K., Mizutani, M., Namikawa, T., Tsudzuki, M., 2007. High genetic divergence in miniature breeds of japanese native chickens compared to red junglefowl, as revealed by microsatellite analysis. Animal Genetics, 39: 71-78.
- Wilkinson, S., Wiener, P., Teverson, D., Haleyand, C.S., Hocking, P.M., 2011. Characterization of the genetic diversity, structure and admixture of British chicken breeds. Animal Genetics, 43: 552-563.
- Wright, S., 1951, The Genetical Structure of Populations. Annals of Eugenics, 15: 323-354.
- Wright, S., 1969. Evolution and Genetics of Populations: The Theory of Gene Frequencies. Univ. Chicago Press, Chicago.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Agricultural Biotechnology |
| Authors | |
| Publication Date | June 25, 2019 |
| Acceptance Date | February 14, 2019 |
| Published in Issue | Year 2019 Volume: 34 Issue: 2 |
