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Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae)

Year 2023, Volume: 38 Issue: 2, 397 - 406, 04.07.2023

Gülru Yücel Ebru Cambaz Nurşen Çördük

https://doi.org/10.7161/omuanajas.1286961
Cited By: 1

Abstract

The study aimed to determine the chromosome number and nuclear DNA content of an endemic plant, Verbascum scamandri, using 14-week-old plants germinated from seed and to determine the genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 1 mg/L 2,4-D and 9-week-old propagated plants, induced and developed on MS medium containing 2 mg/L Kin + 0.1 mg/L 2,4-D. In the mitotic chromosome counts showed that V. scamandri had 2n = 32 chromosome number. Flow cytometric analysis revealed that 14-week-old in vitro grown plants have 0.73 pg/2C ± 0.01, callus tissue has 0.76 pg/2C ± 0.02 and propagated plantlets have 0.79 pg/2C ± 0.01. The results proved that propagated plants had similar DNA content to the seed-derived plants which showed analysed plants were genetically stable.

Keywords

Nuclear DNA Content Chromosome Tissue Culture Mullein

Supporting Institution

Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit, Çanakkale, Türkiye

Project Number

FYL-2021- 3732

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Verbascum scamandri Murb. (Scrophulariaceae) Türünün Çekirdek DNA İçeriğinin ve Kromozom Sayısının Belirlemesi

Year 2023, Volume: 38 Issue: 2, 397 - 406, 04.07.2023

Gülru Yücel Ebru Cambaz Nurşen Çördük

https://doi.org/10.7161/omuanajas.1286961
Cited By: 1

Abstract

Çalışmanın amacı, endemik bir bitki olan Verbascum scamandri türünün in vitro büyütülen 14 haftalık bitkiler kullanılarak kromozom sayısı ve çekirdek DNA içeriğinin belirlenmesi, 2 mg/L Kin + 0.1 mg/L 2,4-D içeren MS ortamında çoğaltılan 9 haftalık in vitro bitkilerde ve 1 mg/L Kin + 1 mg/L 2,4-D içeren MS ortamında indüklenen kallus dokularında flow sitometri analizleri ile genetik kararlılığın tespit edilmesidir. Mitoz kromozom sayımlarında türün kromozom sayısının 2n = 32 olduğu belirlenmiştir. Flow sitometri analizinde, in vitro yetiştirilen bitki örneklerinin 0.73 pg/2C ± 0.01, kallus örneklerinin 0.76 pg/2C ± 0.02, in vitro çoğaltılmış bitkiciklerin DNA içeriği ise 0.79 pg/2C ± 0.01 belirlenmiştir. Yapılan flow sitometri analizleri tohumdan gelişen bitkiler ile in vitro çoğaltılan bitkilerin benzer çekirdek DNA içeriklerine sahip olduklarını göstermiş ve dolayısıyla genetik stabil oldukları anlaşılmıştır.

Keywords

Çekirdek DNA İçeriği Kromozom Doku Kültürü Sığırkuyruğu

Project Number

FYL-2021- 3732

References

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  • Çördük, N., Yücel, G., Akıncı, N., Tuna, M., Esen, O., 2018. In vitro propagation of Silene bolanthoides Quézel, Contandr. & Pamukç. and assessment of genetic stability by flow cytometry. Archives of Biological Sciences, 70(1): 141-148. https://doi.org/10.2298/ABS170410033C.
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  • Duman, H., Uzunhisarcıklı, M.E., Tan, K., 2017. Verbascum mughlaeum (Scrophulariaceae), a new species from SW Anatolia, Turkey. Phytotaxa, 291(3): 231-236. https://doi.org/10.11646/phytotaxa.291.3.8
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  • Escobedo-Gracia-Medrano, R.M., Burgos-Tan, M.J., Ku-Cauich, J.R., Quiroz-Moreno, A., 2018. Using Flow Cytometry Analysis in Plant Tissue Culture Derived Plants. Methods in Molecular Biology, 1815: 317-332. https://doi.org/10.1007/978-1-4939-8594-4_22.
  • Esposito, E., Dal Toso, R., Pressi, G. Bramanti, P., Meli, R., Cuzzocrea, S., 2010. Protective effect of verbascoside in activated C6 glioma cells: possible molecular mechanisms. Naunyn-Schmiedeberg's Archives of Pharmacology, 381: 93-105. https://doi.org/10.1007/s00210-009-0466-0
  • Galbraith, D.W., 2009. Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry A., 75(8): 692-8. https://doi.org/10.1002/cyto.a.20760.
  • Hacıoğlu Doğru, N., Demir, N., Yılmaz, Ö., 2021. Three species of Verbascum L. from Northwest Anatolia of Turkey as a source of biological activities. Turk J Anal Chem, 3(1): 19-26. https://doi.org/10.51435/turkjac.886692
  • Heywood, V.H., 1993. Flowering Plants of the World. Oxford University Press.
  • Huber-Morath, A., 1978. Verbascum L In: Davis PH (ed) Flora of Turkey and the East Aegean Islands. Vol 6. Edinburgh University Press Edinburgh. pp 461-603.
  • Hung, J. Y., Yang, C. J., Tsai, Y. M., Huang, H. W., Huang, M. S., 2008. Antiproliferative activity of aucubin is through cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells. Clinical and Experimental Pharmacology and Physiology, 35(9): 995-1001. https://doi.org/10.1111/j.1440-1681.2008.04935.x
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  • Karavelioğulları, F.A., 2015b. Verbascum misirdalianum (Scrophulariaceae), a new species from central Anatolia, Turkey. Phytotaxa, 217(1): 96-99. https://doi.org/10.11646/phytotaxa.217.1.10
  • Kevers, C., Greimers, R., Franck, T., Bisbis, T., Dommes, J., Gaspar, T., 1999. Flow cytometry estimation of nuclear size and ploidy level of habituated calli of sugar beet. Biologia Plantarum, 42(3): 321–332. https://doi.org/10.1023/A:1002469331895
  • Kozan, E., Çankaya, I.T., Kahraman, C., Akkol, E.K., Akdemir, Z., 2011. The in vivo anthelmintic efficacy of some Verbascum species growing in Turkey. Experimental Parasitology, 129(2): 211-214. https://doi.org/10.1016/j.exppara.2011.06.005
  • Kubalaková, M., Dolezel, J., Lebeda, A., 1996. Ploidy instability of embryogenic cucumber (Cucumis sativus L.) callus culture. Biologia Plantarum, 38(3): 475–480. https://doi.org/10.1007/BF02896685.
  • Kupeli, E., Tatli, I.I., Akdemir, Z.S., Yesilada, E. 2007. Bioas- say-guided isolation of anti-inflammatory and antinociceptive glycoterpenoids from the flowers of Verbascum lasianthum Boiss. ex Bentham, J. Ethnopharmacol., 110, 444-450., 2007. https://doi.org/10.1016/j.jep.2006.10.004
  • Lee, Y.I., Tseng, Y-F., Lee, Y.C., Chung, M.C., 2020. Chromosome constitution and nuclear DNA content of Phalaenopsis hybrids. Scientia Horticulturae, 262. 109089.https://doi.org/10.1016/j.scienta.2019.109089.
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There are 56 citations in total.

Details

Primary Language English
Subjects Agricultural Molecular Engineering of Nucleic Acids and Proteins
Journal Section Anadolu Tarım Bilimleri Dergisi
Authors

Gülru Yücel 0000-0001-9785-929X

Ebru Cambaz 0000-0001-7301-9288

Nurşen Çördük 0000-0001-8499-4847

Project Number FYL-2021- 3732
Early Pub Date June 30, 2023
Publication Date July 4, 2023
Acceptance Date May 23, 2023
Published in Issue Year 2023 Volume: 38 Issue: 2

Cite

APA Yücel, G., Cambaz, E., & Çördük, N. (2023). Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae). Anadolu Tarım Bilimleri Dergisi, 38(2), 397-406. https://doi.org/10.7161/omuanajas.1286961
AMA Yücel G, Cambaz E, Çördük N. Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae). ANAJAS. July 2023;38(2):397-406. doi:10.7161/omuanajas.1286961
Chicago Yücel, Gülru, Ebru Cambaz, and Nurşen Çördük. “Determination of Nuclear DNA Content and Chromosome Number of Verbascum Scamandri Murb. (Scrophulariaceae)”. Anadolu Tarım Bilimleri Dergisi 38, no. 2 (July 2023): 397-406. https://doi.org/10.7161/omuanajas.1286961.
EndNote Yücel G, Cambaz E, Çördük N (July 1, 2023) Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae). Anadolu Tarım Bilimleri Dergisi 38 2 397–406.
IEEE G. Yücel, E. Cambaz, and N. Çördük, “Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae)”, ANAJAS, vol. 38, no. 2, pp. 397–406, 2023, doi: 10.7161/omuanajas.1286961.
ISNAD Yücel, Gülru et al. “Determination of Nuclear DNA Content and Chromosome Number of Verbascum Scamandri Murb. (Scrophulariaceae)”. Anadolu Tarım Bilimleri Dergisi 38/2 (July 2023), 397-406. https://doi.org/10.7161/omuanajas.1286961.
JAMA Yücel G, Cambaz E, Çördük N. Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae). ANAJAS. 2023;38:397–406.
MLA Yücel, Gülru et al. “Determination of Nuclear DNA Content and Chromosome Number of Verbascum Scamandri Murb. (Scrophulariaceae)”. Anadolu Tarım Bilimleri Dergisi, vol. 38, no. 2, 2023, pp. 397-06, doi:10.7161/omuanajas.1286961.
Vancouver Yücel G, Cambaz E, Çördük N. Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae). ANAJAS. 2023;38(2):397-406.

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