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Obesity Induces Oxidative Stress By ACSL4 And GPX4-Mediated Ferroptosis

Year 2022, Volume: 44 Issue: 2, 224 - 230, 28.02.2022
https://doi.org/10.20515/otd.986897

Abstract

In this study, the parameters of oxidative stress markers and ferroptosis were evaluated in experimental groups of adult individuals, which were formed according to the body mass index (BMI). A total of 39 adult patients were normal weight or overweight (BMI: 18.5-29.9), first degree obese (BMI 30-34.9) and second degree obese (BMI: 35-39.9) were divided into three groups of thirteen each.. From the serum samples of healthy individuals in the control group and patients in the obesity groups, malondialdehyde (MDA), total antioxidant capacity (TAS), total oxidant capacity (TOS), glutathione (GSH), Acyl-CoA synthetase long chain family member 4 (ACSL4) and glutathione peroxidase 4 (GPx4) levels were measured. Compared to the control group, TOS levels increased in grade 1 and grade 2 obese patients (p<0.0001), while TAS levels decreased in grade 1 obese and grade 2 obese patients (p<0.001). MDA levels were increased in grade 1 obese (p<0.001) and grade 2 obese patients (p<0.0001) compared to the control group. GSH levels decreased as the degree of obesity increased (p<0.001). Compared to the control group, ACSL4 levels of 1st and 2nd obese patients increased (p<0.001), while GPx4 levels decreased in both obese groups (p<0.001). As the degree of obesity increases, lipid peroxidation and oxidative stress increase. In addition, when the decrease in GPx4 levels and the increase in ACSL4 levels in obese individuals compared to the control group are evaluated together, it can be thought that obesity induces ferroptosis.

References

  • 1. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González Á, Esquivel-Chirino C et al. Inflammation, oxidative stress, and obesity. International journal of molecular sciences, 2011;12(5):3117-3132.
  • 2. Manna P, Jain SK. Obesity, oxidative stress, adipose tissue dysfunction, and the associated health risks: causes and therapeutic strategies. Metabolic syndrome and related disorders, 2015;13(10):423-444.

Obezite ACSL4 ve GPX4 Aracılı Ferroptozis ile Oksidatif Stresi İndükler

Year 2022, Volume: 44 Issue: 2, 224 - 230, 28.02.2022
https://doi.org/10.20515/otd.986897

Abstract

Bu çalışmada, vücut kitle indeksine (VKİ) göre oluşturulmuş yetişkin bireylerden oluşan deney gruplarında oksidatif stres belirteçleri ve ferroptoz parametreleri toplam 39 yetişkin birey normal kilolu veya hafif kilolu (VKİ:18.5-29.9), birinci derece obez (VKİ 30-34.9) ve ikinci derecede obez (VKİ:35-39,9) olmak üzere on üçer kişilik üç gruba ayrılmıştır. Kontrol grubundaki sağlıklı bireylerin ve obezite gruplarındaki hastaların serum örneklerinden malondialdehit (MDA) ,total antioksidan kapasite (TAS), total oksidan kapasite (TOS), glutatyon (GSH), asil-CoA sentetaz uzun zincirli aile üyesi 4 (ACSL4) ve glutatyon peroksidaz 4 (GPx4) seviyeleri ölçülmüştür. TOS seviyeleri, kontrol grubu ile karşılaştırıldığında 1. derece obez ve 2. derece obez hastalarda artarken (p<0.0001) TAS seviyeleri, 1. derece obez ve 2. derece obez hastalarda azalmıştır (p<0.001). MDA seviyeleri, kontrol grubuna kıyasla 1. derece obez (p<0,001) ve 2. derece obez hastalarda(p <0,0001) artmıştır. GSH seviyeleri ise obezite derecesi arttıkça düşüş göstermiştir (p<0.001). Kontrol grubuna kıyasla 1. derece obez ve 2. derece obez hastaların ACSL4 seviyeleri artış gösterirken (p<0.001), GPx4 seviyeleri her iki obez gupta da azalmıştır (p<0.001). Obezite derecesi arttıkça lipit peroksidasyonu ve oksidatif stres artmaktadır. Ek olarak kontrol grubuna kıyasla obez bireylerde GPx4 seviyelerinin azalışı ve ACSL4 seviyelerinin artışı birlikte değerlendirildiğinde obezitenin ferroptozu indüklediği düşünülebilir.

References

  • 1. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González Á, Esquivel-Chirino C et al. Inflammation, oxidative stress, and obesity. International journal of molecular sciences, 2011;12(5):3117-3132.
  • 2. Manna P, Jain SK. Obesity, oxidative stress, adipose tissue dysfunction, and the associated health risks: causes and therapeutic strategies. Metabolic syndrome and related disorders, 2015;13(10):423-444.
There are 2 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section ORİJİNAL MAKALE
Authors

Gülay Sezgin 0000-0001-6309-7725

Fatih Kar 0000-0001-8356-9806

Ceyhan Hacıoğlu 0000-0002-0993-6118

Sema Uslu 0000-0003-0670-9187

Publication Date February 28, 2022
Published in Issue Year 2022 Volume: 44 Issue: 2

Cite

Vancouver Sezgin G, Kar F, Hacıoğlu C, Uslu S. Obezite ACSL4 ve GPX4 Aracılı Ferroptozis ile Oksidatif Stresi İndükler. Osmangazi Tıp Dergisi. 2022;44(2):224-30.

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