The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato

Dursun Kısa

doi:10.19113/sdufbed.52379

The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato

Year 2018, Volume: 22 Issue: 1, 1 - 6, 16.04.2018

Dursun Kısa

https://doi.org/10.19113/sdufbed.52379

Cited By: 6

Abstract

Plants maintain their life cycles under the various environmental conditions such as oxidative stress induced by heavy metals. Accumulation of metal ions in plants causes the formation of free radicals and stimulates the antioxidative defense systems. In this study, the activities of APX, POD, and SOD are investigated in the leaves and roots of tomato cultivated under the heavy metal-induced stress. The activities of APX, POD, and SOD exhibited remarkable induction with the treatment of Cd, Cu and Pb (10, 20 and 50 ppm) in the leaves of tomato compared to control plants except for 50 ppm Pb. In roots, APX activity changed depending on the heavy metal types and concentrations, while Cd clearly increased it with stress conditions, but Cu decreased in tomato compared to control. The activity of POD clearly exhibited that the all doses of heavy metals reduced the enzyme activity in roots polluted with heavy metals. The treatment of Cd (10, 20 and 50 ppm) significantly increased the activity of SOD, however, Cu showed the opposite effect which significantly decreased with increasing doses in roots compared to uncontaminated plants. Also, roots from plants grown on the high concentration of Pb (20 and 50 ppm) induced the activity of SOD. Briefly, it is clear responses which Cd significantly raised the activities of APX and SOD in leaves and roots of tomato. The decreases caused by these metals in the activity of POD and Cu in the activities of APX and SOD in roots of tomato can be clarified by the result of heavy metal-induced the over production of free radical.

Keywords

Heavy metal, APX; Tomato; POD; SOD

References

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[2] Sharma, S.S., Dietz, K.J. 2009. The relationship between metal toxicity and cellular redox imbalance. Trends in Plant Science, 14(2009), 43–50.
[3] Aydin, S.S., Gökçe, E., Büyük, İ., Aras, S. 2012. Characterization of stress induced by copper and zinc on cucumber (Cucumis sativus L.) seedlings by means of molecular and population parameters. Mutation Research, 746(2012), 49– 55.
[4] Ovecka, M., Takac, T. 2014. Managing heavy metal toxicity stress in plants: Biological and biotechnological tools. Biotechnology Advances, 32 (2014), 73–86.
[5] Pál, M., Horváth, E., Janda, T., Páldi, E., Szalai, G. 2006. Physiological changes and defense mechanisms induced by cadmium stress in maize. Journal of Plant Nutrition and Soil Science, 169(2006), 239–46.
[6] Jayakumar, K., Jaleel, C.A., Vijayarengan, P. 2007. Changes in growth, biochemical constituents, and antioxidant potentials in radish (Raphanus sativus L.) under cobalt stress, Turkish Journal of Biology. 31(2007), 127–36.
[7] Kisa, D., Elmastaş, M., Öztürk, L., Kayır, Ö. 2016. Responses of the phenolic compounds of Zea mays under heavy metal stress. Applied Biological Chemistry, 59(2016), 813–20.
[8] Bhaduri, A.M., Fulekar, M.H. 2012. Antioxidant enzyme responses of plants to heavy metal stress. Reviews in Environmental Science and Biotechnology, 11(2012), 55–69.
[9] Bajguz, A., Hayat, S. 2009. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiology and Biochemistry, Elsevier Masson SAS; 47(2009), 1–8.
[10] Sobrino-Plata, J., Meysen, D., Cuypers, A., Escobar, C., Hernandez, L.E. 2014. Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, 377(2014), 369–381.
[11] Haribabu, T., Sudha, P. 2011. Effect of heavy metals copper and cadmium exposure on the antioxidant properties of the plant Cleome gynandra. International Journal of Plant, Animal and Environmental Sciences, 1(2011), 80–7.
[12] Gratao, P.L., Monteiro, C.C., Tezotto, T., Carvalho, R.F., Alves, L.R., Peters, L.P., Azevedo, R.A. 2015. Cadmium stress antioxidant responses and root-to-shoot communication in grafted tomato plants. Biometals, 28(2015), 803–816.
[13] Nakano, Y., Asada, K. 1981. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts, 22(1981), 867–80.
[14] Angelini, R., Manes, F., Federico, R. 1990. Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chickpea stems. Planta, 182(1990), 89–96.
[15] Beyer, W.F., Fridovich, I. 1987. Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry, 161(1987), 559–66.
[16] Gratao, P.L., Monteiro, C.C., Antunes, A.M., Peres LEP, Azevedo RA. 2008. Acquired tolerance of tomato (Lycopersicon esculentum cv. Micro-Tom) plants to cadmium-induced stress. Annals of Applied Biology, 153(2008), 321–33.
[17] Gupta, D.K., Nicoloso, F.T., Schetinger, M.R.C., Rossato, L.V., Pereira, L.B., Castro, G.Y. 2009.. Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. Journal of Hazardous Materials, 172(2009), 479–84.
[18] Fatima, R.A., Ahmad, M. 2005. Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater. Science of the Total Environment, 346(2005), 256–73.
[19] Romero-Puertas, M.C., Corpas, F.J., Rodríguez-Serrano, M., Gómez, M, del Río. L.A., Sandalio, L.M. 2007. Differential expression and regulation of antioxidative enzymes by cadmium in pea plants. Journal of Plant Physiology, 164(2007), 1346–57.
[20] Martins, L.L., Mourato, M.P. 2006. Effect of excess copper on tomato plants: Growth parameters, enzyme activities, chlorophyll, and mineral content. Journal of Plant Nutrition, 29(2006), 2179–98.
[21] Shah, K., Kumar, R.G., Verma, S., Dubey, R.S. 2001. Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Science, 161(2001), 1135–44.
[22] Cherif, J., Mediouni, C., Ammar, W., Ben, Jemal, F. 2011. Interactions of zinc and cadmium toxicity in their effects on growth and in antioxidative systems in tomato plants (Solarium lycopersicum). Journal of Environmental Sciences. The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 23(2011), 837–44.
[23] Ekmekçi, Y., Tanyolaç, D., Ayhan, B. 2008. Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. Journal of Plant Physiology, 165(2008), 600–11.
[24] Stobrawa, K., Lorenc-Plucińska, G. 2007. Changes in antioxidant enzyme activity in the fine roots of black poplar (Populus nigra L.) and cottonwood (Populus deltoides Bartr. ex Marsch) in a heavy-metal-polluted environment. Plant and Soil, 298(2007), 57–68.
[25] Stobrawa, K., Lorenc-Plucińska, G. 2008. Thresholds of heavy-metal toxicity in cuttings of European black poplar (Populus nigra L.) determined according to antioxidant status of fine roots and morphometrical disorders. Science of the Total Environment, 390(2008), 86–96.

Year 2018, Volume: 22 Issue: 1, 1 - 6, 16.04.2018

Dursun Kısa

https://doi.org/10.19113/sdufbed.52379

Cited By: 6

Abstract

References

[1] Nagajyoti, P.C., Lee, K.D., Sreekanth, T.V.M. 2010. Heavy metals, occurrence and toxicity for plants: A review. Environmental Chemistry Letters, 8(2010), 199–216.
[2] Sharma, S.S., Dietz, K.J. 2009. The relationship between metal toxicity and cellular redox imbalance. Trends in Plant Science, 14(2009), 43–50.
[3] Aydin, S.S., Gökçe, E., Büyük, İ., Aras, S. 2012. Characterization of stress induced by copper and zinc on cucumber (Cucumis sativus L.) seedlings by means of molecular and population parameters. Mutation Research, 746(2012), 49– 55.
[4] Ovecka, M., Takac, T. 2014. Managing heavy metal toxicity stress in plants: Biological and biotechnological tools. Biotechnology Advances, 32 (2014), 73–86.
[5] Pál, M., Horváth, E., Janda, T., Páldi, E., Szalai, G. 2006. Physiological changes and defense mechanisms induced by cadmium stress in maize. Journal of Plant Nutrition and Soil Science, 169(2006), 239–46.
[6] Jayakumar, K., Jaleel, C.A., Vijayarengan, P. 2007. Changes in growth, biochemical constituents, and antioxidant potentials in radish (Raphanus sativus L.) under cobalt stress, Turkish Journal of Biology. 31(2007), 127–36.
[7] Kisa, D., Elmastaş, M., Öztürk, L., Kayır, Ö. 2016. Responses of the phenolic compounds of Zea mays under heavy metal stress. Applied Biological Chemistry, 59(2016), 813–20.
[8] Bhaduri, A.M., Fulekar, M.H. 2012. Antioxidant enzyme responses of plants to heavy metal stress. Reviews in Environmental Science and Biotechnology, 11(2012), 55–69.
[9] Bajguz, A., Hayat, S. 2009. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiology and Biochemistry, Elsevier Masson SAS; 47(2009), 1–8.
[10] Sobrino-Plata, J., Meysen, D., Cuypers, A., Escobar, C., Hernandez, L.E. 2014. Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, 377(2014), 369–381.
[11] Haribabu, T., Sudha, P. 2011. Effect of heavy metals copper and cadmium exposure on the antioxidant properties of the plant Cleome gynandra. International Journal of Plant, Animal and Environmental Sciences, 1(2011), 80–7.
[12] Gratao, P.L., Monteiro, C.C., Tezotto, T., Carvalho, R.F., Alves, L.R., Peters, L.P., Azevedo, R.A. 2015. Cadmium stress antioxidant responses and root-to-shoot communication in grafted tomato plants. Biometals, 28(2015), 803–816.
[13] Nakano, Y., Asada, K. 1981. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts, 22(1981), 867–80.
[14] Angelini, R., Manes, F., Federico, R. 1990. Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chickpea stems. Planta, 182(1990), 89–96.
[15] Beyer, W.F., Fridovich, I. 1987. Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Analytical Biochemistry, 161(1987), 559–66.
[16] Gratao, P.L., Monteiro, C.C., Antunes, A.M., Peres LEP, Azevedo RA. 2008. Acquired tolerance of tomato (Lycopersicon esculentum cv. Micro-Tom) plants to cadmium-induced stress. Annals of Applied Biology, 153(2008), 321–33.
[17] Gupta, D.K., Nicoloso, F.T., Schetinger, M.R.C., Rossato, L.V., Pereira, L.B., Castro, G.Y. 2009.. Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. Journal of Hazardous Materials, 172(2009), 479–84.
[18] Fatima, R.A., Ahmad, M. 2005. Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater. Science of the Total Environment, 346(2005), 256–73.
[19] Romero-Puertas, M.C., Corpas, F.J., Rodríguez-Serrano, M., Gómez, M, del Río. L.A., Sandalio, L.M. 2007. Differential expression and regulation of antioxidative enzymes by cadmium in pea plants. Journal of Plant Physiology, 164(2007), 1346–57.
[20] Martins, L.L., Mourato, M.P. 2006. Effect of excess copper on tomato plants: Growth parameters, enzyme activities, chlorophyll, and mineral content. Journal of Plant Nutrition, 29(2006), 2179–98.
[21] Shah, K., Kumar, R.G., Verma, S., Dubey, R.S. 2001. Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Science, 161(2001), 1135–44.
[22] Cherif, J., Mediouni, C., Ammar, W., Ben, Jemal, F. 2011. Interactions of zinc and cadmium toxicity in their effects on growth and in antioxidative systems in tomato plants (Solarium lycopersicum). Journal of Environmental Sciences. The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 23(2011), 837–44.
[23] Ekmekçi, Y., Tanyolaç, D., Ayhan, B. 2008. Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. Journal of Plant Physiology, 165(2008), 600–11.
[24] Stobrawa, K., Lorenc-Plucińska, G. 2007. Changes in antioxidant enzyme activity in the fine roots of black poplar (Populus nigra L.) and cottonwood (Populus deltoides Bartr. ex Marsch) in a heavy-metal-polluted environment. Plant and Soil, 298(2007), 57–68.
[25] Stobrawa, K., Lorenc-Plucińska, G. 2008. Thresholds of heavy-metal toxicity in cuttings of European black poplar (Populus nigra L.) determined according to antioxidant status of fine roots and morphometrical disorders. Science of the Total Environment, 390(2008), 86–96.

There are 25 citations in total.

Details

Journal Section	Articles
Authors	Dursun Kısa
Publication Date	April 16, 2018
Published in Issue	Year 2018 Volume: 22 Issue: 1

Cite

APA	Kısa, D. (2018). The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(1), 1-6. https://doi.org/10.19113/sdufbed.52379
AMA	Kısa D. The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato. SDÜ Fen Bil Enst Der. April 2018;22(1):1-6. doi:10.19113/sdufbed.52379
Chicago	Kısa, Dursun. “The Responses of Antioxidant System Against the Heavy Metal-Induced Stress in Tomato”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22, no. 1 (April 2018): 1-6. https://doi.org/10.19113/sdufbed.52379.
EndNote	Kısa D (April 1, 2018) The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 1 1–6.
IEEE	D. Kısa, “The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato”, SDÜ Fen Bil Enst Der, vol. 22, no. 1, pp. 1–6, 2018, doi: 10.19113/sdufbed.52379.
ISNAD	Kısa, Dursun. “The Responses of Antioxidant System Against the Heavy Metal-Induced Stress in Tomato”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/1 (April 2018), 1-6. https://doi.org/10.19113/sdufbed.52379.
JAMA	Kısa D. The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato. SDÜ Fen Bil Enst Der. 2018;22:1–6.
MLA	Kısa, Dursun. “The Responses of Antioxidant System Against the Heavy Metal-Induced Stress in Tomato”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 22, no. 1, 2018, pp. 1-6, doi:10.19113/sdufbed.52379.
Vancouver	Kısa D. The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato. SDÜ Fen Bil Enst Der. 2018;22(1):1-6.