Research Article
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The Stimulation of Chemical Male Sterility for F1 Hybrid Lettuce (Lactuca Sativa Var. Longifolia) Production

Year 2024, Volume: 38 Issue: 1, 41 - 52, 30.04.2024

Arif Selim Arıcı Ertan Sait Kurtar

Abstract

Male sterility is a unique application in the production of F1 hybrid seeds of some important species. Today, while F1 hybrid lettuce varieties of abroad origin take their place in the Türkiye seed market, unfortunately, we do not have a native F1 hybrid variety. Besides, F1 hybrid seed production has become a prestige for multi-national companies regardless of the type of vegetable. Within this perspective, the effects of some chemical hybridizing agents (CHAs) such as E4FO, Ethrel and GA3 on male sterilizing activity in lettuce cultivars (Maylight352 F1, Presidential and Yedikule) were evaluated. Therefore, pollen presence in the early bud stage, seed formation and seed viability (germination) were examined. The applications had different effects on pollen presence, seed formation and seed germination, and thus male sterility. Ethrel was not effective at low doses, but at high doses, it caused flower bud deformation and growth retardation. E4FO is partially effective, but the application doses are low. Therefore, E4FO should be used at higher than 1500 ppm and 2000 ppm. GA3 applications produced the best results in stimulating male sterility, and full sterility (% 100) was achieved from 200, 250 and 300 ppm in all cultivars. As a result, 200 ppm GA3 was determined as the recommendable dose in the production of Fı hybrid lettuce.

Keywords

: Male Sterility Gametocytes (CHAs) Lettuce

Supporting Institution

This research was funded by the Scientific Research Projects Coordination Office of Selcuk University, Turkey.

Project Number

Project No.20201079

References

  • Ali AJ, Devakumar C, Zaman F, Siddiq E (1999). Identification of potent gametocides for selectiveinduction of male sterility in rice. Indian Journal of Genetics and Plant Breeding, 59 (04): 429-436.
  • Altındal D (2019). Effect of gibberellic acid (GA3) application on male sterility in barley (Hordeum vulgare L.). Pakistan Journal of Botany, 51(6): DOI: http://dx.doi.org/10.30848/PJB2019-6(40).
  • Amelework A, Laing M, Shimelis H (2016). Evaluation of effective gametocides for selective induction of male sterility in sorghum. Czech Journal of Genetics and Plant Breeding, 52 (4): 163-170.
  • Ananthi M, Selvaraju P, Srimathi P (2013). Transgenic male sterility for hybrid seed production in vegetables-A review. Weekly Sci. Res. Journal 1 (16): 1-10.
  • Balkaya A, Özgen R (2019). The place and economic importance of lettuce cultivation in agricultural production in Turkey. Tarım Gündem Dergisi, [13.05.2022].
  • Baydar H (2000). Effect of gibberellic acid on male sterility, seed yield, and fat and fatty acid synthesis in safflower (Carthamus tinctorius L.). Turkish Journal of Biology 24: 159-168.
  • Billore M (2015). Male-sterility and its exploitation. Genetic Improvement of Field Crops, 254.
  • Chakraborty K, Devakumar C (2005). N-acylanilines, herbicide− CHA chimera, and amino acid analogues as novel chemical hybridizing agents for wheat (Triticum aestivum L.). Journal of Agricultural and Food Chemistry, 53 (20): 7899-7907.
  • Chakraborty K, Devakumar C (2006). Ethyloxanilates as specific male gametocides for wheat (Triticum aestivum L.). Plant Breeding 125 (5): 441-447.
  • Collantes ER, Xing L, Miller PC, Welsh WJ, Profeta S (1999). Comparative molecular field analysis as a tool to evaluate mode of action of chemical hybridization agents. Journal of Agricultural and Food Chemistry, 47 (12): 5245-5251.
  • Colombo N, Galmarini CR (2017). The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review. Plant Breeding, 136 (3): 287-299.
  • Curtis IS, He C, Scott R, Power JB, Davey MR (1996). Genomic male sterility in lettuce, a baseline for the production of F1 hybrids. Plant Science, 113 (1): 113-119.
  • Devakumar C (2006). Evaluation of chemical compounds for induction of male sterility in wheat (Triticum aestivum L.). Euphytica, 147 (3): 329-335.
  • Dotlacil L, Apltauerová M (1978). Pollen sterility induced by ethrel and its utilization in hybridization of wheat. Euphytica, 27 (2): 353-360.
  • Duca M, Port A, Orozco-Cardenas M, Lovatt C (2008). Gibberellin-induced gene expression associated with cytoplasmic male sterility in sunflower. Biotechnology & Biotechnological Equipment, 22 (2): 691-698.
  • Eenink A (1977). Induction of male sterility in lettuce (Lactuca sativa L.) by application of gibberellic acid: a technical note. Euphytica, 26(1): 31-32.
  • Eenink A, Vereijken A (1978). Anatomical changes in flower buds of lettuce (Lactuca sativa L.) treated with a GA3-solution for induction of male sterility. Acta Botanica Neerlandica, 27 (3/4): 199-204.
  • Eşiyok D (2012). Winter and summer vegetable cultivation. (In Turkish) Ege University, Agriculture Faculty, Horticulture Department, 410 p.
  • Feráková V (1977). Genus Lactuca L. in Europe. Univerzita Komenského, p. 88-94.
  • Ghebrehiwot H, Burgdorf R, Hussein S, Laing M (2015). The efficacy of four gametocides for induction of pollen sterility in Eragrostis tef (Zucc.) Trotter. African Journal of Biotechnology, 14 (9): 774-780.
  • Goubara M, Takasaki T (2004). Pollination effects of the sweat bee Lasioglossum villosulum trichopse (Hymenoptera: Halictidae) on genic male-sterile lettuce. Applied Entomology and Zoology, 39 (1): 163-169.
  • Hayashi M, Ujiie A, Serizawa H, Sassa H, Kakui H, Oda T, Koba T (2011). Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.). Euphytica, 180 (3): 429-436.
  • Huang S, Cerny RE, Qi Y, Bhat D, Aydt CM, Hanson DD, Malloy KP, Ness LA (2003). Transgenic studies on the involvement of cytokinin and gibberellin in male development. Plant Physiology, 131 (3): 1270-1282.
  • Hussain SM, Hussain K, Farwah S, Rizvi S, Rashid M, Saleem S, Andrabi N, Rashid H (2018). Male sterility in vegetable crops. Journal of Pharmacognosy and Phytochemistry, 7 (3): 3390-3393.
  • ISTA (2009). International Rules for Seed Testing. The International Seed Testing Association (ISTA), Zurichstr. 50, CH-8303, Bassersdorf, Switzerland.
  • Karaağaç O, Balkaya A (2019). Varieties used in today's production and their properties. Tarım Gündem Dergisi, [13.05.2022].
  • Khatib P, Olfati CJA, Hamidoghli Y (2016). Efficiency of different lettuce (Lactuca sativa L.) emasculation methods for hybrid seed production. Seed and Plant Journal, 32(1):141-146.
  • Ma BL, Smith DL (1992). Post-anthesis ethephon effects on yield of spring barley. American Society of Agronomy, 84: 370-374.
  • Michel H, Soussin T (2014). Production of hybrid Lactuca sativa seeds. US Patent, Patent No: US 8,716,551 B2.
  • Nagata R (1992). Clip-and-wash method of emasculation for lettuce. HortScience, 27 (8): 907-908.
  • Nelson PM, Rossman E (1958). Chemical induction of male sterility in inbred maize by use of gibberellins. Science, 127 (3313): 1500-1501.
  • Prohens J, Nuez F (2008). Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Handbook of Plant Breeding, DOI: https://doi.org/10.1007/978-0-387-30443-4.
  • Ryder EJ (1999). Lettuce, endive and chicory. USDA-ARS, Salinas, CA 93905, USA. ISBN: 9780851992853.
  • Şalk A, Arın L, Deveci M, Polat S (2008). Special vegetable book. (In Turkish). Tekirdağ: Namık Kemal University Agriculture Faculty, Horticulture Department, 488 p.
  • Takada K, Watanabe S, Sano T, Ma B, Kamada H, Ezura H (2007). Heterologous expression of the mutated melon ethylene receptor gene Cm-ERS1/H70A produces stable sterility in transgenic lettuce (Lactuca sativa). Journal of Plant Physiology, 164 (4): 514-520.
  • Thompson RC (1958). Natural cross-pollination in lettuce. Proc. Am. Soc. Hort. Sci., 403-409.
  • Tinna D (2019). Chemically induced male sterility in hybrid breeding of vegetables: A review. Journal of Pharmacognosy and Phytochemistry, 8: 430-434.
  • Yılmaz Mİ (2010). Investigation of male sterility effects of some growth regulators on hybrid seed production in sunflower (Helianthus annuus L.). MSc Thesis, Namık Kemal University, Turkey.
  • Yılmaz C, Yetişir H, Sarı N (2002). The effects of some chemical treatments on head formation in lital lettuce (Lactuca sativa L.). Alatarım, 1 (2): 36-41.

Year 2024, Volume: 38 Issue: 1, 41 - 52, 30.04.2024

Arif Selim Arıcı Ertan Sait Kurtar

Abstract

Project Number

Project No.20201079

References

  • Ali AJ, Devakumar C, Zaman F, Siddiq E (1999). Identification of potent gametocides for selectiveinduction of male sterility in rice. Indian Journal of Genetics and Plant Breeding, 59 (04): 429-436.
  • Altındal D (2019). Effect of gibberellic acid (GA3) application on male sterility in barley (Hordeum vulgare L.). Pakistan Journal of Botany, 51(6): DOI: http://dx.doi.org/10.30848/PJB2019-6(40).
  • Amelework A, Laing M, Shimelis H (2016). Evaluation of effective gametocides for selective induction of male sterility in sorghum. Czech Journal of Genetics and Plant Breeding, 52 (4): 163-170.
  • Ananthi M, Selvaraju P, Srimathi P (2013). Transgenic male sterility for hybrid seed production in vegetables-A review. Weekly Sci. Res. Journal 1 (16): 1-10.
  • Balkaya A, Özgen R (2019). The place and economic importance of lettuce cultivation in agricultural production in Turkey. Tarım Gündem Dergisi, [13.05.2022].
  • Baydar H (2000). Effect of gibberellic acid on male sterility, seed yield, and fat and fatty acid synthesis in safflower (Carthamus tinctorius L.). Turkish Journal of Biology 24: 159-168.
  • Billore M (2015). Male-sterility and its exploitation. Genetic Improvement of Field Crops, 254.
  • Chakraborty K, Devakumar C (2005). N-acylanilines, herbicide− CHA chimera, and amino acid analogues as novel chemical hybridizing agents for wheat (Triticum aestivum L.). Journal of Agricultural and Food Chemistry, 53 (20): 7899-7907.
  • Chakraborty K, Devakumar C (2006). Ethyloxanilates as specific male gametocides for wheat (Triticum aestivum L.). Plant Breeding 125 (5): 441-447.
  • Collantes ER, Xing L, Miller PC, Welsh WJ, Profeta S (1999). Comparative molecular field analysis as a tool to evaluate mode of action of chemical hybridization agents. Journal of Agricultural and Food Chemistry, 47 (12): 5245-5251.
  • Colombo N, Galmarini CR (2017). The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review. Plant Breeding, 136 (3): 287-299.
  • Curtis IS, He C, Scott R, Power JB, Davey MR (1996). Genomic male sterility in lettuce, a baseline for the production of F1 hybrids. Plant Science, 113 (1): 113-119.
  • Devakumar C (2006). Evaluation of chemical compounds for induction of male sterility in wheat (Triticum aestivum L.). Euphytica, 147 (3): 329-335.
  • Dotlacil L, Apltauerová M (1978). Pollen sterility induced by ethrel and its utilization in hybridization of wheat. Euphytica, 27 (2): 353-360.
  • Duca M, Port A, Orozco-Cardenas M, Lovatt C (2008). Gibberellin-induced gene expression associated with cytoplasmic male sterility in sunflower. Biotechnology & Biotechnological Equipment, 22 (2): 691-698.
  • Eenink A (1977). Induction of male sterility in lettuce (Lactuca sativa L.) by application of gibberellic acid: a technical note. Euphytica, 26(1): 31-32.
  • Eenink A, Vereijken A (1978). Anatomical changes in flower buds of lettuce (Lactuca sativa L.) treated with a GA3-solution for induction of male sterility. Acta Botanica Neerlandica, 27 (3/4): 199-204.
  • Eşiyok D (2012). Winter and summer vegetable cultivation. (In Turkish) Ege University, Agriculture Faculty, Horticulture Department, 410 p.
  • Feráková V (1977). Genus Lactuca L. in Europe. Univerzita Komenského, p. 88-94.
  • Ghebrehiwot H, Burgdorf R, Hussein S, Laing M (2015). The efficacy of four gametocides for induction of pollen sterility in Eragrostis tef (Zucc.) Trotter. African Journal of Biotechnology, 14 (9): 774-780.
  • Goubara M, Takasaki T (2004). Pollination effects of the sweat bee Lasioglossum villosulum trichopse (Hymenoptera: Halictidae) on genic male-sterile lettuce. Applied Entomology and Zoology, 39 (1): 163-169.
  • Hayashi M, Ujiie A, Serizawa H, Sassa H, Kakui H, Oda T, Koba T (2011). Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.). Euphytica, 180 (3): 429-436.
  • Huang S, Cerny RE, Qi Y, Bhat D, Aydt CM, Hanson DD, Malloy KP, Ness LA (2003). Transgenic studies on the involvement of cytokinin and gibberellin in male development. Plant Physiology, 131 (3): 1270-1282.
  • Hussain SM, Hussain K, Farwah S, Rizvi S, Rashid M, Saleem S, Andrabi N, Rashid H (2018). Male sterility in vegetable crops. Journal of Pharmacognosy and Phytochemistry, 7 (3): 3390-3393.
  • ISTA (2009). International Rules for Seed Testing. The International Seed Testing Association (ISTA), Zurichstr. 50, CH-8303, Bassersdorf, Switzerland.
  • Karaağaç O, Balkaya A (2019). Varieties used in today's production and their properties. Tarım Gündem Dergisi, [13.05.2022].
  • Khatib P, Olfati CJA, Hamidoghli Y (2016). Efficiency of different lettuce (Lactuca sativa L.) emasculation methods for hybrid seed production. Seed and Plant Journal, 32(1):141-146.
  • Ma BL, Smith DL (1992). Post-anthesis ethephon effects on yield of spring barley. American Society of Agronomy, 84: 370-374.
  • Michel H, Soussin T (2014). Production of hybrid Lactuca sativa seeds. US Patent, Patent No: US 8,716,551 B2.
  • Nagata R (1992). Clip-and-wash method of emasculation for lettuce. HortScience, 27 (8): 907-908.
  • Nelson PM, Rossman E (1958). Chemical induction of male sterility in inbred maize by use of gibberellins. Science, 127 (3313): 1500-1501.
  • Prohens J, Nuez F (2008). Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Handbook of Plant Breeding, DOI: https://doi.org/10.1007/978-0-387-30443-4.
  • Ryder EJ (1999). Lettuce, endive and chicory. USDA-ARS, Salinas, CA 93905, USA. ISBN: 9780851992853.
  • Şalk A, Arın L, Deveci M, Polat S (2008). Special vegetable book. (In Turkish). Tekirdağ: Namık Kemal University Agriculture Faculty, Horticulture Department, 488 p.
  • Takada K, Watanabe S, Sano T, Ma B, Kamada H, Ezura H (2007). Heterologous expression of the mutated melon ethylene receptor gene Cm-ERS1/H70A produces stable sterility in transgenic lettuce (Lactuca sativa). Journal of Plant Physiology, 164 (4): 514-520.
  • Thompson RC (1958). Natural cross-pollination in lettuce. Proc. Am. Soc. Hort. Sci., 403-409.
  • Tinna D (2019). Chemically induced male sterility in hybrid breeding of vegetables: A review. Journal of Pharmacognosy and Phytochemistry, 8: 430-434.
  • Yılmaz Mİ (2010). Investigation of male sterility effects of some growth regulators on hybrid seed production in sunflower (Helianthus annuus L.). MSc Thesis, Namık Kemal University, Turkey.
  • Yılmaz C, Yetişir H, Sarı N (2002). The effects of some chemical treatments on head formation in lital lettuce (Lactuca sativa L.). Alatarım, 1 (2): 36-41.
There are 39 citations in total.

Details

Primary Language English
Subjects Vegetable Growing and Treatment
Journal Section Research Article
Authors

Arif Selim Arıcı 0000-0002-2138-511X

Ertan Sait Kurtar 0000-0002-7203-7430

Project Number Project No.20201079
Early Pub Date April 30, 2024
Publication Date April 30, 2024
Submission Date June 14, 2023
Published in Issue Year 2024 Volume: 38 Issue: 1

Cite

EndNote Arıcı AS, Kurtar ES (April 1, 2024) The Stimulation of Chemical Male Sterility for F1 Hybrid Lettuce (Lactuca Sativa Var. Longifolia) Production. Selcuk Journal of Agriculture and Food Sciences 38 1 41–52.
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