DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)

Serkan Örtücü; Nurcan Albayrak İskender

doi:10.23902/trkjnat.285656

Research Article

Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ

Year 2017, Volume: 18 Issue: 1, 33 - 38, 08.04.2017

Serkan Örtücü Nurcan Albayrak İskender

https://doi.org/10.23902/trkjnat.285656

Cited By: 1

Abstract

Entomopatojen fungusların geniş konukçu çeşitliliği
biyolojik mücadele endüstrisi için ticari bir avantaj olabilir. Bununla
birlikte, entomopatojen fungusların tümü konukçuya nüfuz etmek için çeşitli
enzimlerinin kombinasyonlarını kullanır ve bu enzimler fungal izolatların
konukçu çeşitliliğinde önemli bir rol oynar. Bu çalışmanın amacı, üç Beauveria bassiana (Bals.) Vull.
izolatının Tetranychus urticae Koch'a
(Acari: Tetranychidae) karşı kontrol potansiyellerinin ve lipaz, kitinaz ve
proteaz enzim aktivitelerinin belirlenmesidir. Bu amaçla, daha önce Coleoptera,
Lepidoptera ve Hymenoptera'ya karşı entomopatojenik aktivite gösteren B. bassiana (PaF04, PaF09 ve PaF76)
izolatları seçilmiştir. Test organizması olarak, T. urticae kullanılmıştır. Üç farklı dozda süspansiyon (1x10⁶,
1x10⁷ve 1x10⁸ konidi ml^-1) Tween 80'de
hazırlanmıştır. 2ml tek doz spor süspansiyonu el spreyi kullanılarak fasülye
yaprak disklerinin alt yüzüne püskürtülmüştür. T. urticae’nın ölüm oranları 5 gün boyunca günlük olarak
kaydedilmiştir. Enzim aktiviteleri petri plate yöntemi ile araştırılmış ve
koloni çevresinde oluşan açık zonlar, enzimatik aktivitenin varlığını
göstermiştir. Test edilen tüm fungus izolatları uygulamadan 5 gün sonra,
64,2±5,6% ve 100% arasında mortaliteye yol açarak T. urticae’ye karşı patojenite göstermiştir. LT50 değerleri
3,16±0,2 ve 3,72±0,5 gün arasında değişmiştir. PaF04 izolatının neden olduğu
mortalite önemli derecede farklı bulunmuştur (p<0,05). Sonuçta, B. bassiana PaF izolatlarının
Coleoptera, Lepidoptera ve Hymenoptera'yı kontrol potansiyellerinin olduğu
kadar T. urticae’yı baskılama
potansiyeline de sahip oldukları belirlenmiştir. Mevcut sonuçlar, kullanılan
izolatların, özellikle de PaF04'ün geniş bir konukçu aralığına sahip olduğunu
göstermiştir.

Keywords

Beauveria bassiana, geniş konukçu aralığı, Tetranychus urticae, enzim aktivitesi

References

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2. Albayrak İskender, N., Ortucu, S., Aksu, Y. & Saral, A. 2017. Isolation, characterization and pathogenicity of fungi from Pristiphora abietina (Hymenoptera: Tenthredinidae). Fresenius Environmental Bulletin, 26(1a): 628–633.
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6. Belczewski, R. & Harmsen, R. 2000. The effect of non-pathogenic phylloplane fungi on life-history traits of urticae (Acari: Tetranychidae). Experimental and Applied Acarology, 24(4): 257–270.
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DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)

Year 2017, Volume: 18 Issue: 1, 33 - 38, 08.04.2017

Serkan Örtücü Nurcan Albayrak İskender

https://doi.org/10.23902/trkjnat.285656

Cited By: 1

Abstract

Broad host range of entomopathogenic fungi may
have a commercial advantage for biological control industry. In addition, all
entomopathogenic fungi rely on a combination of different enzymes to penetrate
their hosts and these enzymes play an important role in the host range of
fungal isolates. The aim of this study is to determine the potential of three Beauveria bassiana (Bals.) Vull.
isolates in control of Tetranychus
urticae Koch (Acari: Tetranychidae) and to determine their lipase,
chitinase, and protease activities. For this purpose, B. bassiana (PaF04, PaF09 and PaF76) isolates, which was previously
demonstrated to have entomopathogenic activity against Coleoptera, Lepidoptera,
and Hymenoptera were chosen. T. urticae
was used as the test organisms. Three different suspensions (1x10⁶,
1x10⁷ and 1x10⁸ conidia ml^-1) were prepared in
Tween 80. 2ml suspension of a single dose was sprayed onto down sides of bean
leaf discs using hand sprayer. Mortality rates of T. urticae, in terms of LT50 values, were recorded daily for 5 days.
Enzyme activities were investigated by petri plate assays and clear zones
formed around the colonies indicated enzymatic activities. All tested fungal
isolates were pathogenic to the T.
urticae causing mortality between 64.2±5.6% and 100% on the 5^thday
after the application. LT50 values varied between 3.16±0.2 and 3.72±0.5 days.
Mortality caused by PaF04 isolate was found to be significantly
different (p<0.05). In conclusion, PaF isolates of B. bassiana have been determined to have potential to suppress T. urticae, in addition to their
potential in control of Coleoptera, Lepidoptera, and Hymenoptera. The present
results showed that the isolates used, especially PaF04, have a broad host
range.

Keywords

Beauveria bassiana, broad host range, Tetranychus urticae, enzyme activity

References

1. Abd-Aziz, S., Sin, T.L., Alitheen, N., Shahab, N. & Kamaruddin, K. 2008. Microbial degradation of chitin materials by Trichoderma virens UKM1. Journal of Biological Sciences, 8(1): 52–59.
2. Albayrak İskender, N., Ortucu, S., Aksu, Y. & Saral, A. 2017. Isolation, characterization and pathogenicity of fungi from Pristiphora abietina (Hymenoptera: Tenthredinidae). Fresenius Environmental Bulletin, 26(1a): 628–633.
3. Albayrak İskender, N., Ortucu, S., Kılıc, E. & Aksu, Y. 2013. Assessment of Beauveria bassiana strains isolated from various insects to control Dendroctonus micans (Kugel.) larvae, 4th International Participated Entomopathogens and Microbial Control Symposium, 11-14 September, Artvin, 97.
4. Albayrak İskender, N., Örtücü, S. & Aksu, Y. 2012. Pathogenicity of three isolates of the entomopathogenic fungi Beauveria bassiana to control Hyphantria cunea (Drury) (Lepidoptera:Arctiidae) larvae. Kırgızistan-Türkiye Manas Üniversitesi Fen Bilimleri Dergisi, 13: 15–21.
5. Alves, S.B., Tamai, M.A., Rossi, L.S. & Castiglioni, E. 2005. Beauveria bassiana pathogenicity to the citrus rust mite Phyllocoptruta oleivora. Experimental and Applied Acarology, 37(1–2): 117–122.
6. Belczewski, R. & Harmsen, R. 2000. The effect of non-pathogenic phylloplane fungi on life-history traits of urticae (Acari: Tetranychidae). Experimental and Applied Acarology, 24(4): 257–270.
7. Brodeur, J. 2012. Host specificity in biological control: Insights from opportunistic pathogens. Evolutionary Applications, 5(5): 470–480.
8. Bugeme, D.M., Knapp, M., Boga, H.I., Wanjoya, A.K. & Maniania, N.K. 2009. Influence of temperature on virulence of fungal ısolates of Metarhizium anisopliae and Beauveria bassiana to the two-spotted spider mite Tetranychus urticae. Mycopathologia, 167(4): 221–227.
9. Bugeme, D.M., Maniania, N.K., Knapp, M. & Boga, H.I. 2008. Effect of temperature on virulence of Beauveria bassiana and Metarhizium anisopliae isolates to Tetranychus evansi. Experimental and Applied Acarology, 46(1–4): 275–285.
10. Butt, T.M. 2002. Use of Entomogenous Fungi for the Control of Insect Pests, 111–134. in: (Kempken, F.), Agricultural Applications, Springer Berlin Heidelberg, Berlin, 388.
11. Campos, R.A., Arruda, W., Boldo, J.T., Silva, M.V., de Barros, N.M., de Azevedo, J.L., Schrank, A. & Vainstein, M.H. 2005. Boophilus microplus infection by Beauveria amorpha and Beauveria bassiana: SEM analysis and regulation of subtilisin-like proteases and chitinases. Current Microbiology, 50(5): 257–261.
12. Charnley, A.K. & Collins, S.A. 2007. Entomopathogenic fungi and their role in pest control, 159–187. in (Kubicek, C.P. & Druzhinina, I.S.), Environmental and Microbial Relationships, Heidelberg: Springer, Berlin, 350.
13. Cherry, A.J., Abalo, P. & Hell, K. 2005. A laboratory assessment of the potential of different strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) to control Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) in stored cowpea. Journal of Stored Products Research, 41(3): 295–309.
14. Clarkson, J.M. & Charnley, A.K. 1996. New insights into the mechanisms of fungal pathogenesis in insects. Trends in Microbiology, 4(5): 197–203.
15. Draganova, S.A. & Simova, S.A. 2010. Susceptibility of Tetranychus urticae Koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana. Pesticides and Phytomedicine, 25(1): 51–57.
16. Eken, C. & Hayat, R. 2009. Preliminary evaluation of Cladosporium cladosporioides (Fresen.) de Vries in laboratory conditions, as a potential candidate for biocontrol of Tetranychus urticae Koch. World Journal of Microbiology and Biotechnology, 25(3): 489–492.
17. Ekesi, S., Maniania, N. K. & Lwande, W. 2000. Susceptibility of the legume flower thrips to Metarhizium anisopliae on different varieties of cowpea. BioControl, 45(1):79–95.
18. Feng, M.G. & Johnson, J.B. 1990. Relative virulence of six isolates of Beauveria bassiana on Diuraphis noxia (Homoptera: Aphididae). Environmental Entomology, 19: 3(3): 785–790.
19. Feng, M.G., Poprawski, T.J. & Khachatourians, G.G. 1994. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol Science and Technology, 4(1): 3–34.
20. Geroh, M., Gulati, R. & Tehri, K. 2015. Determination of lethal concentration and lethal time of entomopathogen Beauveria bassiana ( Balsamo ) Vuillemin against Tetranychus urticae Koch. International Journal of Agriculture Sciences, 7(5): 523–528.
21. Goettel, M.S., Poprawski, T.J., Vandenberg, J.D. & Roberts, D.W. 1990. Safety to non target invertebrates of fungal biocontrol agents, 209–231. in (Laird, M., Lacey, L. & Davidson, E.), Safety of Microbial Insecticides, CRC Press, Boca Raton, 272.
22. Gupta, S.C., Leathers, T.D., Elsayed, G.N. & Ignoffo, C.M. 1994. Relationships among enzyme activities and virulence parameters in Beauveria bassiana infections of Galleria mellonella and Trichoplusia ni. Journal of Invertebrate Pathology, 64(1): 13–17.
23. Hatting, J.L., Wraight, S.P. & Miller, R.M. 2004. Efficacy of Beauveria bassiana (Hyphomycetes) for control of Russian wheat aphid (Homoptera: Aphididae) on resistant wheat under field conditions. Biocontrol Science and Technology, 14(5): 459–473.
24. Kady, G.A, Sharabasy, H.M., Mahmoud, M.F., Bahgat, I.M. & Said, P. 2007. Toxicity of two potential bio-insecticides against moveable stages of Tetranychus urticae Koch. Journal of Applied Sciences, 3(11): 1315–1319.
25. Khan, S., Guo, L., Shi, H., Mijit, M. & Qiu, D. 2012. Bioassay and enzymatic comparison of six entomopathogenic fungal isolates for virulence or toxicity against green peach aphids Myzus persicae. African Journal of Biotechnology, 11(77): 14193–14203.
26. Leger, R.S. & Screen, S. 2001. Prospects for strain improvement of fungal pathogens of insects and weeds, 219–237. in: (Butt, T., Jackson, C. & Magan, N.), Fungi as Biocontrol Agents Progress, Problems and Potential, CABI, Wallingford, 416.
27. van Lenteren, J.C. 2012. The state of commercial augmentative biological control: plenty of natural enemies, but a frustrating lack of uptake. BioControl, 57(1): 1–20.
28. Mascarin, G.M., Kobori, N.N., Quintela, E.D. & Delalibera, I. 2013. The virulence of entomopathogenic fungi against Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) and their conidial production using solid substrate fermentation. Biological Control, 66(3): 209–218.
29. Meyling, N., Lubeck, M., Buckley, E., Eilenberg, J. & Rehner, S. 2009. Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Molecular Ecology, 18(6): 1282–1293.
30. Numa Vergel, S.J., Bustos, R.A., Rodríguez, C.D. & Cantor, R.F. 2011. Laboratory and greenhouse evaluation of the entomopathogenic fungi and garlic–pepper extract on the predatory mites, Phytoseiulus persimilis and Neoseiulus californicus and their effect on the spider mite Tetranychus urticae. Biological Control, 57(2): 143–149.
31. Ortiz-Urquiza, A. & Keyhani, N. 2013. Action on the surface: entomopathogenic fungi versus the insect cuticle. Insects, 4(3): 357–374.
32. Ownley, B.H., Griffin, M.R., Klingeman, W.E., Gwinn, K.D., Moulton, J.K. & Pereira, R.M. 2008. Beauveria bassiana: endophytic colonization and plant disease control. Journal of Invertebrate Pathology, 98(3): 267–270.
33. Ownley, B.H., Pereira, R.M., Klingeman, W.E., Quigley, N.B. & Leckie, B.M. 2004. Beauveria bassiana, a dual purpose biocontrol organism, with activity against insect pests and plant pathogens, 255–269. in: (Lartey, R. & Caesar, A.), Emerging Concepts in Plant Health Management, Research Signpost, Kerala, 298.
34. Quesada-Moraga, E., Maranhao, E.A.A., Valverde-García, P. & Santiago-Álvarez, C. 2006. Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements, and toxicogenic activity. Biological Control, 36(3): 274–287.
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There are 46 citations in total.

Details

Subjects	Structural Biology
Journal Section	Research Article/Araştırma Makalesi
Authors	Serkan Örtücü Nurcan Albayrak İskender
Publication Date	April 8, 2017
Submission Date	January 12, 2017
Acceptance Date	March 15, 2017
Published in Issue	Year 2017 Volume: 18 Issue: 1

Cite

APA	Örtücü, S., & Albayrak İskender, N. (2017). DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya University Journal of Natural Sciences, 18(1), 33-38. https://doi.org/10.23902/trkjnat.285656
AMA	Örtücü S, Albayrak İskender N. DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya Univ J Nat Sci. June 2017;18(1):33-38. doi:10.23902/trkjnat.285656
Chicago	Örtücü, Serkan, and Nurcan Albayrak İskender. “DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria Bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus Urticae KOCH (ACARI: TETRANYCHIDAE)”. Trakya University Journal of Natural Sciences 18, no. 1 (June 2017): 33-38. https://doi.org/10.23902/trkjnat.285656.
EndNote	Örtücü S, Albayrak İskender N (June 1, 2017) DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya University Journal of Natural Sciences 18 1 33–38.
IEEE	S. Örtücü and N. Albayrak İskender, “DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)”, Trakya Univ J Nat Sci, vol. 18, no. 1, pp. 33–38, 2017, doi: 10.23902/trkjnat.285656.
ISNAD	Örtücü, Serkan - Albayrak İskender, Nurcan. “DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria Bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus Urticae KOCH (ACARI: TETRANYCHIDAE)”. Trakya University Journal of Natural Sciences 18/1 (June 2017), 33-38. https://doi.org/10.23902/trkjnat.285656.
JAMA	Örtücü S, Albayrak İskender N. DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya Univ J Nat Sci. 2017;18:33–38.
MLA	Örtücü, Serkan and Nurcan Albayrak İskender. “DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria Bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus Urticae KOCH (ACARI: TETRANYCHIDAE)”. Trakya University Journal of Natural Sciences, vol. 18, no. 1, 2017, pp. 33-38, doi:10.23902/trkjnat.285656.
Vancouver	Örtücü S, Albayrak İskender N. DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya Univ J Nat Sci. 2017;18(1):33-8.

Cited By

A Laboratory Assessment of Two Local Strains of the Beauveria bassiana (Bals.) Vuill. against the Tetranychus urticae (Acari: Tetranychidae) and Their Potential as a Mycopesticide

Journal of Pathogens

Serkan Ortucu

https://doi.org/10.1155/2017/7628175

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