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Amsonia orientalis’de 6-Benzilaminopürin Destekli In vitro Sürgün Çoğaltımı Üzerine Kuraklık Stresinin Morfolojik ve Fizyolojik Etkileri

Year 2019, Volume: 6 Issue: 3, 372 - 378, 23.07.2019
https://doi.org/10.30910/turkjans.595149

Abstract

Bu çalışmada Amsonia orientalis Decne.’nin 6-benzilaminopürin (BAP) destekli in vitro sürgün çoğaltımı aşamasında
uygulanmış kuraklık stresi etkisinin morfolojik ve bazı fizyolojik etkileri
incelenmiştir. Kültürlerin
inkübasyon sürelerinin sonunda sürgünlerin ortalama uzunluk ve sayıları, H2O2
(hidrojen peroksit) ve MDA (malondialdehit) seviyeleri ile SOD (süperoksit
dismutaz), POD (peroksidaz) ve CAT (katalaz) enzimlerinin aktiviteleri analiz
edilmiştir. Polietilen glikol
(PEG-6000) kullanılarak elde edilen -0,15, -0,49, -1,03 ve -1,76 MPa osmotik
potansiyeller sürgünler üzerinde BAP ilaveli ve ilavesiz olmak üzere iki
kontrol grubu kullanılarak test edilmiştir.
Ortalama sürgün sayısı düşen osmotik potansiyelle birlikte BAP ilaveli
kontrole kıyasla azalsa da BAP ilavesiz kontrol değeriyle istatistiki olarak
aynı düzeyde kalmıştır. Ortalama
sürgün uzunluğu ise sadece en şiddetli kuraklık uygulaması sonucu BAP ilavesiz
kontrol değerinin altına düşmüştür. SOD
aktivitesi artan kuraklığa bağlı olarak kontrol değerlerine göre önemli ölçüde
azalırken şiddetli kuraklıkta
kontrol değerine kadar yükselmiştir. POD
ve CAT aktivitelerinde de düşüş gözlenmiş ancak CAT aktivitesindeki azalmanın
daha belirgin olduğu görülmüştür. Artan
kuraklık etkisinde H2O2 ve MDA seviyelerinin benzer olarak
arttığı bulunmuştur. Buna karşın bu
artış düşük seviyelerde gerçekleşmiştir.
Kuraklık stresi uygulanmamışken antioksidan enzim aktivitelerinin daha
yüksek bulunması fakat düşen osmotik potansiyellerde beklenenin aksine azalması
durumu A. orientalis’te BAP
uygulamasının hücresel osmotik düzenleyicilerin görev aldığı enzimatik olmayan
savunma mekanizmasını uyardığını ve daha etkin kullanılmasına imkân verdiği
sonucunu düşündürmüştür. Bu nedenle bitkide kuraklık stresinde BAP
uygulamasının moleküler ve fizyolojik işleyişinin detaylı olarak ortaya konması
gerekmektedir. BAP uygulaması doğada A.
orientalis
’in kuraklık stresine maruz kalması durumunda kuraklığın
sürgünler üzerindeki olası olumsuz etkilerini hafifletmek için tercih
edilebilir.

Thanks

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından desteklenmiştir (113Z609).

References

  • Acemi, A., Özen, F., Kıran, R. 2012. Development of an efficient callus production protocol for Amsonia orientalis: A critically endangered medicinal plant. Eurasian Journal of Biosciences, 6: 105-112.
  • Acemi, A., Özen, F., Kıran, R. 2013. In vitro propagation of Amsonia orientalis Decne. from nodal segments of adult plants. Propagation of Ornamental Plants, 13: 25-32.
  • Acemi, A., Duruksu, G., Özen, F. 2017a. Cytostatic effects of methanolic extracts of Amsonia orientalis Decne. on MCF-7 and DU145 cancer cell lines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1): 36-42.
  • Acemi, A., Duman, Y., Karakuş, Y.Y., Kömpe, Y.Ö., Özen, F. 2017b. Analysis of plant growth and biochemical parameters in Amsonia orientalis after in vitro salt stress. Horticulture, Environment, and Biotechnology, 58: 231-239.
  • Acemi, A., Duman, Y., Karakuş, Y.Y., Özen, F. 2018. Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.). Folia Horticulturae, 30(2): 405-414.
  • Aebi, H. 1974. Methods of enzymatic analysis. “Alınmıştır: Catalase. (ed) Bergmeyer, H.U., Academic Press, New York, 673-675.
  • Anjum, S.A., Xie, X., Wang, L., Saleem, M.F., Man, C., Lei, W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6: 2026-2032.
  • Beauchamp, C.O., Fridovich, I. 1973. Isozymes of superoxide dismutase from wheat germ. Biochimica et Biophysica Acta, 317: 50-64.
  • Bradford, M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
  • Catola, S., Marino, G., Emiliani, G., Huseynova, T., Musayev, Y., Akparov, Z., Maserti, B.E., 2016. Physiological and metabolomic analysis of Punica granatum (L.) under drought stress. Planta, 243(2): 441-449.
  • Doupis, G., Chartzoulakis, K., Beis, A., Patakas, A. 2011. Allometric and biochemical responses of grapevines subjected to drought and enhanced ultraviolet-B radiation. Australian Journal of Grape and Wine Research, 17: 36-42.
  • Duman, Y.A., Acemi, A., Toygar, H.İ., Yüzügüllü, Y., Özen, F. 2016. Tuz stresi ve BAP varlığında Amsonia orientalis’in antioksidan enzimlerinin incelenmesi, Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 12(3): 543-551.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A. 2009. Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212.
  • George, E.F. 2008. Plant propagation by tissue culture. Springer (3rd ed), Dordrecht.
  • Gurmani, A.R., Bano, A., Khan, S.U., Din, J., Zhang, J.L. 2011. Alleviation of salt stress by seed treatment with abscisic acid (ABA), 6-benzylaminopurine (BA) and chlormequat chloride (CCC) optimizes ion and organic matter accumulation and increases yield of rice (Oryza sativa L.). Australian Journal of Crop Science, 5: 1278-1285.
  • Gürkanlı, C.T., Özkoç, İ., Aydın, E.B., Acemi, A., Özen, F. 2014. Genetic diversity of Amsonia orientalis. Biologia, 69: 742-749.
  • Kotov, A.A., Kotova, L.M. 2015. Role of acropetal water transport in regulation of cytokinin levels in stems of pea seedlings. Russian Journal of Plant Physiology, 62(3): 390-400.
  • Kumari, S., Kumar, S., Prakash, P. 2018. Exogenous application of cytokinin (6-BAP) ameliorates the adverse effect of combined drought and high temperature stress in wheat seedling. Journal of Pharmacognosy and Phytochemistry, 7(1): 1176-1180.
  • Mohammadkhani, N., Heidari, R. 2008. Effects of drought stress on soluble proteins in two maize varieties. Turkish Journal of Biology, 32: 23-30.
  • Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497.
  • Rivero, R.M., Kojima M., Gepstein, A., Sakakibara, H., Mittler, R., Gepstein, S., Blumwald, E. 2007. Delayed leaf senescence induces extreme drought tolerance in a flowering plant. Proceedings of the National Academy of Sciences of The United States of America, 104: 19631-19636.
  • Robert-Seilaniantz A., Bari R., Jones, J.D.G. 2010. A Biotic or Abiotic Stress?. “Alınmıştır: Abiotic Stress Adaptation In Plants. (ed) Pareek A., Sopory S.K., Bohnert H.J., Govindjee, Springer, Dordrecht, The Netherlands, 103-117.
  • Rulcová, J., Pospíšilová, J. 2001. Effect of benzylaminopurine on rehydration of bean plants after water stress. Biologia Plantarum, 44: 75-81.
  • Sedghi, M., Sharifi, R.S., Pirzad, A.R., Amanpour-Balaneji, B. 2012. Phytohormonal regulation of antioxidant systems in petals of drought stressed pot marigold (Calendula officinalis L.). Journal of Agricultural Science and Technology, 14: 869-878.
  • Skórzyńska-Polit, E. 2007. Lipid Peroxidation in plant cells, its physiological role and changes in heavy metal stress. Acta Societatis Botanicorum Poloniae, 76(1): 49-54.
  • Targovnik, A.M., Cascone, O., Miranda, M.V. 2012. Extractive purification of recombinant peroxidase isozyme c from insect larvae in aqueous two-phase systems. Separation and Purification Technology, 98: 199-205.
  • Turan, S., Tripathy, B.C. 2013. Salt and genotype impact on antioxidative enzymes and lipid peroxidation in two rice cultivars during de-etiolation. Protoplasma, 250: 209-222.
  • Valetti, N.W., Picó, G. 2013. A friendly method for Raphanus sativus L. (wild radish) peroxidase purification by polyelectrolyte precipitation. Seperation and Purification Technology, 119: 1-6.
  • Wu, G.Q., Zhang, L.N., Wang, Y.Y., 2012. Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings. Plant, Soil and Environment, 58(12): 534-539.
  • Xu, Q., Lu, Y., Jing, L., Cai, L., Zhu, X., Xie, J., Hu, X. 2014. Specific binding and inhibition of 6-benzylaminopurine to catalase: Multiple spectroscopic methods combined with molecular docking study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 123: 327-335.
  • Yüzügüllü Karakuş, Y., Toygar, H.İ., Acemi, A., Avcı Duman, Y., Özen, F. 2018. UV-C stresinin 6-benzilaminopürin varlığında çoğaltılmış Amsonia orientalis’in in vitro sürgün gelişimi ve antioksidan enzimleri üzerine etkileri. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 18: 44-52.

Morphological and Physiological Effects of Drought Stress on 6-Benzylaminopurine-Assisted In vitro Shoot Multiplication in Amsonia orientalis

Year 2019, Volume: 6 Issue: 3, 372 - 378, 23.07.2019
https://doi.org/10.30910/turkjans.595149

Abstract

In this study, the morphological and physiological effects of drought
stress on Amsonia orientalis Decne. were
investigated during 6-benzylaminopurine
(BAP) -assisted in vitro shoot
multiplication. At the end of the incubation period, mean shoot lengths and
numbers, H2O2 (hydrogen peroxide) and MDA
(malondialdehyde) levels and the activities of SOD (superoxide dismutase), POD
(peroxidase) and CAT (catalase) enzymes were
analyzed. The osmotic potentials of -0.15, -0.49, -1.03, and -1.76 MPa
obtained using polyethylene glycol (PEG-6000) were tested on shoots, and the
results were compared with two control groups, with and without BAP. Although
the mean number of shoots declined with decreasing osmotic potential compared
to control with BAP, it remained at the statistically same level with control
without BAP. The mean shoot length was found to be lower than that of the
control without BAP in response to the severe drought application. SOD activity
showed a significant reduction compared to control values ​​in response to mild
drought. However, the activity increased
up to the control values ​​in severe drought. POD and CAT activities also
decreased under drought while the decrease in CAT activity was more pronounced.
H2O2 and MDA levels were found to be increased similarly
with elevated drought stress. However, this increase was found to be limited to
low levels.
The presence of higher antioxidant
enzyme activities in the absence of drought stress but unexpected lower
activities in lower osmotic potentials (during drought stress) suggested that
the application of BAP in A. orientalis
may stimulate the non-enzymatic defense mechanism which employs the cellular
osmotic regulators, and it may allow this mechanism to be used more efficiently
than enzymatic defense system. For this reason, the molecular and physiological
functioning of BAP application in plant drought stress needs to be explained in
detail. Also, BAP application may be preferred to alleviate the potential
adverse effects of drought stress on shoots of A. orientalis in nature.



 

References

  • Acemi, A., Özen, F., Kıran, R. 2012. Development of an efficient callus production protocol for Amsonia orientalis: A critically endangered medicinal plant. Eurasian Journal of Biosciences, 6: 105-112.
  • Acemi, A., Özen, F., Kıran, R. 2013. In vitro propagation of Amsonia orientalis Decne. from nodal segments of adult plants. Propagation of Ornamental Plants, 13: 25-32.
  • Acemi, A., Duruksu, G., Özen, F. 2017a. Cytostatic effects of methanolic extracts of Amsonia orientalis Decne. on MCF-7 and DU145 cancer cell lines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1): 36-42.
  • Acemi, A., Duman, Y., Karakuş, Y.Y., Kömpe, Y.Ö., Özen, F. 2017b. Analysis of plant growth and biochemical parameters in Amsonia orientalis after in vitro salt stress. Horticulture, Environment, and Biotechnology, 58: 231-239.
  • Acemi, A., Duman, Y., Karakuş, Y.Y., Özen, F. 2018. Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.). Folia Horticulturae, 30(2): 405-414.
  • Aebi, H. 1974. Methods of enzymatic analysis. “Alınmıştır: Catalase. (ed) Bergmeyer, H.U., Academic Press, New York, 673-675.
  • Anjum, S.A., Xie, X., Wang, L., Saleem, M.F., Man, C., Lei, W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research, 6: 2026-2032.
  • Beauchamp, C.O., Fridovich, I. 1973. Isozymes of superoxide dismutase from wheat germ. Biochimica et Biophysica Acta, 317: 50-64.
  • Bradford, M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
  • Catola, S., Marino, G., Emiliani, G., Huseynova, T., Musayev, Y., Akparov, Z., Maserti, B.E., 2016. Physiological and metabolomic analysis of Punica granatum (L.) under drought stress. Planta, 243(2): 441-449.
  • Doupis, G., Chartzoulakis, K., Beis, A., Patakas, A. 2011. Allometric and biochemical responses of grapevines subjected to drought and enhanced ultraviolet-B radiation. Australian Journal of Grape and Wine Research, 17: 36-42.
  • Duman, Y.A., Acemi, A., Toygar, H.İ., Yüzügüllü, Y., Özen, F. 2016. Tuz stresi ve BAP varlığında Amsonia orientalis’in antioksidan enzimlerinin incelenmesi, Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 12(3): 543-551.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A. 2009. Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212.
  • George, E.F. 2008. Plant propagation by tissue culture. Springer (3rd ed), Dordrecht.
  • Gurmani, A.R., Bano, A., Khan, S.U., Din, J., Zhang, J.L. 2011. Alleviation of salt stress by seed treatment with abscisic acid (ABA), 6-benzylaminopurine (BA) and chlormequat chloride (CCC) optimizes ion and organic matter accumulation and increases yield of rice (Oryza sativa L.). Australian Journal of Crop Science, 5: 1278-1285.
  • Gürkanlı, C.T., Özkoç, İ., Aydın, E.B., Acemi, A., Özen, F. 2014. Genetic diversity of Amsonia orientalis. Biologia, 69: 742-749.
  • Kotov, A.A., Kotova, L.M. 2015. Role of acropetal water transport in regulation of cytokinin levels in stems of pea seedlings. Russian Journal of Plant Physiology, 62(3): 390-400.
  • Kumari, S., Kumar, S., Prakash, P. 2018. Exogenous application of cytokinin (6-BAP) ameliorates the adverse effect of combined drought and high temperature stress in wheat seedling. Journal of Pharmacognosy and Phytochemistry, 7(1): 1176-1180.
  • Mohammadkhani, N., Heidari, R. 2008. Effects of drought stress on soluble proteins in two maize varieties. Turkish Journal of Biology, 32: 23-30.
  • Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497.
  • Rivero, R.M., Kojima M., Gepstein, A., Sakakibara, H., Mittler, R., Gepstein, S., Blumwald, E. 2007. Delayed leaf senescence induces extreme drought tolerance in a flowering plant. Proceedings of the National Academy of Sciences of The United States of America, 104: 19631-19636.
  • Robert-Seilaniantz A., Bari R., Jones, J.D.G. 2010. A Biotic or Abiotic Stress?. “Alınmıştır: Abiotic Stress Adaptation In Plants. (ed) Pareek A., Sopory S.K., Bohnert H.J., Govindjee, Springer, Dordrecht, The Netherlands, 103-117.
  • Rulcová, J., Pospíšilová, J. 2001. Effect of benzylaminopurine on rehydration of bean plants after water stress. Biologia Plantarum, 44: 75-81.
  • Sedghi, M., Sharifi, R.S., Pirzad, A.R., Amanpour-Balaneji, B. 2012. Phytohormonal regulation of antioxidant systems in petals of drought stressed pot marigold (Calendula officinalis L.). Journal of Agricultural Science and Technology, 14: 869-878.
  • Skórzyńska-Polit, E. 2007. Lipid Peroxidation in plant cells, its physiological role and changes in heavy metal stress. Acta Societatis Botanicorum Poloniae, 76(1): 49-54.
  • Targovnik, A.M., Cascone, O., Miranda, M.V. 2012. Extractive purification of recombinant peroxidase isozyme c from insect larvae in aqueous two-phase systems. Separation and Purification Technology, 98: 199-205.
  • Turan, S., Tripathy, B.C. 2013. Salt and genotype impact on antioxidative enzymes and lipid peroxidation in two rice cultivars during de-etiolation. Protoplasma, 250: 209-222.
  • Valetti, N.W., Picó, G. 2013. A friendly method for Raphanus sativus L. (wild radish) peroxidase purification by polyelectrolyte precipitation. Seperation and Purification Technology, 119: 1-6.
  • Wu, G.Q., Zhang, L.N., Wang, Y.Y., 2012. Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings. Plant, Soil and Environment, 58(12): 534-539.
  • Xu, Q., Lu, Y., Jing, L., Cai, L., Zhu, X., Xie, J., Hu, X. 2014. Specific binding and inhibition of 6-benzylaminopurine to catalase: Multiple spectroscopic methods combined with molecular docking study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 123: 327-335.
  • Yüzügüllü Karakuş, Y., Toygar, H.İ., Acemi, A., Avcı Duman, Y., Özen, F. 2018. UV-C stresinin 6-benzilaminopürin varlığında çoğaltılmış Amsonia orientalis’in in vitro sürgün gelişimi ve antioksidan enzimleri üzerine etkileri. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 18: 44-52.
There are 31 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Arda Acemi

Halil Toygar This is me

Yonca Yüzügüllü Karakuş This is me

Yonca Avcı Duman This is me

Fazıl Özen This is me

Publication Date July 23, 2019
Submission Date October 9, 2018
Published in Issue Year 2019 Volume: 6 Issue: 3

Cite

APA Acemi, A., Toygar, H., Yüzügüllü Karakuş, Y., Avcı Duman, Y., et al. (2019). Amsonia orientalis’de 6-Benzilaminopürin Destekli In vitro Sürgün Çoğaltımı Üzerine Kuraklık Stresinin Morfolojik ve Fizyolojik Etkileri. Türk Tarım Ve Doğa Bilimleri Dergisi, 6(3), 372-378. https://doi.org/10.30910/turkjans.595149