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Investigation of Antimicrobial and Antioxidant Effects of Kılıç Otu Plant (Hypericum perforatum) in Ardahan Region

Year 2020, Volume: 7 Issue: 1, 27 - 31, 25.01.2020
https://doi.org/10.30910/turkjans.679896

Abstract

Hypericum perforatum, which is found in many parts of the world, is known as "kılıç otu" or "sarı kantaron" in our country. The climate of Turkey is the most suitable climate for kılıç otu. It is known to be beneficial in asthma, stomach ulcer, excess acid, lung diseases, and arteriosclerosis and nerve inflammation. In this study the antimicrobial activities and antioxidant capacity of kılıç otu samples which belongs to Hypericaceae family was investigated. The dried plant samples were extracted with ethanol and methanol. Methanol and ethanol extracts of kılıç otu was tested for antimicrobial activity against Pseudomonas aeruginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538), Bacillus megaterium, Escherichia coli, Yarrovia lipolytica, Candida albicans and Saccharomyces cerevisiae. The measurements of glutathione (GSH) levels which participate in vital mechanism of plants were done spectrophometrically and total antioxidant capacity (TAC) was done by ELISA method. The methanolic extract of kılıç otu exhibited higher antibacterial activity than ethanolic extract, but both extracts did not display antifungal effect. GSH levels are various in different solvents. The results showed that the all the studied species have significant results of total antioxidant capacity.

References

  • Altun, M.L., Sever Yılmaz, B., Erdogan Orhan, I., Saltan Citoglu, G. 2013. Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L. (St. John’s wort). Industrial Crops and Products, 43: 87– 92.
  • Behl, C., Mosmann, B. 2002. Antioxidant neuroprotection in Alzheimer’s disease as preventive and therapeutic approach. Free Radical Biology and Medicine, 33: 182–191.
  • Benedí , J., Arroyo, R., Romero, C., Martín-Aragón, S., Villar A.M. 2004. Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. Life Science Journal, 75(10): 1263-76.
  • Collins, C.M., Lyne, P.M., Grange, J.M. 1989. Antimicrobial sensitivity and assay tests. Collins and Lyne’s Microbiological Methods. Butterworths, London.
  • Davis, P.H., Cullen, J. 1984. Hypericaceae. In: Davis, P.H. (Ed.), Flora of Turkey and the East Aegean Islands, vol. 10. Edinburgh University Press, Edinburgh, pp. 96–103.
  • Dost, T., Ozkayran, H., Gokalp, F., Yenisey, C., Birincioğlu M. 2009. The Effect of Hypericum perforatum (St. John’s Wort) on Experimental Colitis in Rat. Digestive Diseases and Sciences, 54:1214–1221.
  • El-Sherbiny, D.A., Khalifa, A.E., Attia, A.S., Eldenshary, E.E.D.S. 2003. Hypericum perforatum extract demonstrates antioxidant properties against elevated rat brain oxidative status induced by amnestic dose of scopolamine. Pharmacology Biochemistry and Behavior, 76(3-4): 525-533.
  • Halliwell, B. 1999. Establishing the significance and optimal intake of dietary antioxidants: the biomarker concept. Nutrition Reviews, 57: 104–113.
  • Heinrich, M., Vikuk, V., Daniels, R., Stintzing, F.C. 2017. Characterization of Hypericum perforatum L. (St. John’s wort) macerates prepared with different fatty oils upon processing and storage. Phytochemistry, Letters 20: 470–480.
  • Lavagna, S.M., Secci, D., Chimenti, P., Bonsignore, L., Ottaviani, A., Bizzarri, B. 2001. Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section. Farmaco, 56: 451-453.
  • Lister, E., Wilson, P. 2001. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity (personel communication). Crop Research Institute Lincoln, New Zealand.
  • Marelli, M., Conforti, F., Toniolo, C., Nicoletti, M., Statti, G., Menichini, F. 2014. Hypericum perforatum : Influences of the habitat on chemical composition, photo-induced cytotoxicity, and antiradical activity. Pharmacological Biology, 52(7): 909–918.
  • Orcic, D.Z., Mimika-Dukic, N.M., Franciskovic, M.M., Petrovic, S.S., Jovin, E.D. 2011. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L. Chemistry Central Journal, 5: 34–42.
  • Orhan, G., Orhan, I., Sener, B. 2006. Recent developments in natural and synthetic drug research for Alzheimer’s disease. Letters in Drug Design and Discovery, 3: 268–274.
  • Orhan, I.E., Kartal, M., Gülpinar, A.R., Cos, P., Matheeussen, A., Maes, L., Tasdemir, D., 2013. Assessment of antimicrobial and antiprotozoal activity of the olive oil macerate samples of Hypericum perforatum and their LC-DAD-MS analyses. Food Chemistry, 138: 870–875.
  • Rahiman, S., Tantry, B.A., Kumar, A. 2013. Variation of Antioxidant Activity and Phenolic Content of Some Common Home Remedies with Storage Time. African Journal of Traditional Complementary and Alternative Medicines, 10(1): 124–127.
  • Sagratini, G., Ricciutelli, M., Vittori, S., Öztürk, N., Öztürk, Y., Maggi, Y. 2008. Phytochemical and antioxidant analysis of eight Hypericum taxa from Central Italy Fitoterapia, 79: 210–213.
  • Sánchez-Reus, M.I., Gómez del Rio, M.A., Iglesias, I., Elorza, M., Slowing, K., Benedí, J. 2007. Standardized Hypericum perforatum reduces oxidative stress and increases gene expression of antioxidant enzymes on rotenone-exposed rats. Neuropharmacology, 52 : 606-616.
  • Sedlak, J., Lindsay, R.H. 1968. Estimation of Total protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry, 25(1): 192-205.
  • Silva, B. A., Ferreres, F., Malva, J. O., Dias, A.C.P. 2005. Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry, 90: 157–167.
  • Süntar, I.P., Akkol, E.K., Yılmazer, D., Baykal, T., Kırmızıbekmez, H., Alper, M., Yeşilada, E. 2010. Investigations on the in vivo wound healing potential of Hypericum perforatum L. Journal of Ethnopharmacology, 127: 468-477.
  • Zheng, W., Wang, S. Y. 2001. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry, 49: 5165–5170.
  • Zou, Y., Lu, Y., Wei, D. 2004. Antioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L. in Vitro. Journal of Agricultural and Food Chemistry, 52(16): 5032-5039.

Ardahan Yöresinde Yetişen Kılıç Otu Bitkisinin (Hypericum perforatum) Antimikrobiyal ve Antioksidan Etkilerinin Araştırılması

Year 2020, Volume: 7 Issue: 1, 27 - 31, 25.01.2020
https://doi.org/10.30910/turkjans.679896

Abstract

Dünyanın birçok yerinde bulunan Hypericum perforatum ülkemizde “kılıç otu” ya da “sarı kantaron” olarak bilinmektedir. Türkiye’nin iklimi kılıç otu için oldukça uygundur. Astım, mide ülseri, midede asit fazlalığı, akciğer hastalıkları, damar sertliği ve sinir iltihaplarında da faydalı olduğu bilinmektedir. Bu çalışmada Ardahan yöresinden toplanan ve Hypericaceae familyasına ait olan kılıç otunun antioksidan ve antimikrobiyal aktiviteleri incelenmiştir. Kurutulmuş bitki numunesi ethanol ve metanol ile ekstrakte edilmiştir. Kılıç otu ethanol ve metanol ekstraktlarının antimikrobiyal aktivitesi Pseudomonas aeruginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538), Bacillus megaterium, Escherichia coli, Yarrovia lipolytica, Candida albicans ve Saccharomyces cerevisiae’ya karşı test edilmiştir. Bitkilerin hayati mekanizmalarına katılan glutatyon (GSH) düzeyi spektrofotometrik olarak, toplam antioksidan kapasiteleri (TAK) ise ELISA yöntemi ile ölçülmüştür. Analizler sonucunda kılıç otu metanol ekstraktında etanol ekstraktına oranla daha yüksek antibakteriyel aktiviteye rastlanırken, her iki ekstraktta da antifungal etki gözlenmemiştir. Örnekler GSH düzeyi bakımından çözücülere göre farklılık göstermiştir. Toplam antioksidan kapasite ölçümlerinde ise anlamlı sonuçlar elde edilmiştir.

References

  • Altun, M.L., Sever Yılmaz, B., Erdogan Orhan, I., Saltan Citoglu, G. 2013. Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L. (St. John’s wort). Industrial Crops and Products, 43: 87– 92.
  • Behl, C., Mosmann, B. 2002. Antioxidant neuroprotection in Alzheimer’s disease as preventive and therapeutic approach. Free Radical Biology and Medicine, 33: 182–191.
  • Benedí , J., Arroyo, R., Romero, C., Martín-Aragón, S., Villar A.M. 2004. Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. Life Science Journal, 75(10): 1263-76.
  • Collins, C.M., Lyne, P.M., Grange, J.M. 1989. Antimicrobial sensitivity and assay tests. Collins and Lyne’s Microbiological Methods. Butterworths, London.
  • Davis, P.H., Cullen, J. 1984. Hypericaceae. In: Davis, P.H. (Ed.), Flora of Turkey and the East Aegean Islands, vol. 10. Edinburgh University Press, Edinburgh, pp. 96–103.
  • Dost, T., Ozkayran, H., Gokalp, F., Yenisey, C., Birincioğlu M. 2009. The Effect of Hypericum perforatum (St. John’s Wort) on Experimental Colitis in Rat. Digestive Diseases and Sciences, 54:1214–1221.
  • El-Sherbiny, D.A., Khalifa, A.E., Attia, A.S., Eldenshary, E.E.D.S. 2003. Hypericum perforatum extract demonstrates antioxidant properties against elevated rat brain oxidative status induced by amnestic dose of scopolamine. Pharmacology Biochemistry and Behavior, 76(3-4): 525-533.
  • Halliwell, B. 1999. Establishing the significance and optimal intake of dietary antioxidants: the biomarker concept. Nutrition Reviews, 57: 104–113.
  • Heinrich, M., Vikuk, V., Daniels, R., Stintzing, F.C. 2017. Characterization of Hypericum perforatum L. (St. John’s wort) macerates prepared with different fatty oils upon processing and storage. Phytochemistry, Letters 20: 470–480.
  • Lavagna, S.M., Secci, D., Chimenti, P., Bonsignore, L., Ottaviani, A., Bizzarri, B. 2001. Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section. Farmaco, 56: 451-453.
  • Lister, E., Wilson, P. 2001. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity (personel communication). Crop Research Institute Lincoln, New Zealand.
  • Marelli, M., Conforti, F., Toniolo, C., Nicoletti, M., Statti, G., Menichini, F. 2014. Hypericum perforatum : Influences of the habitat on chemical composition, photo-induced cytotoxicity, and antiradical activity. Pharmacological Biology, 52(7): 909–918.
  • Orcic, D.Z., Mimika-Dukic, N.M., Franciskovic, M.M., Petrovic, S.S., Jovin, E.D. 2011. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L. Chemistry Central Journal, 5: 34–42.
  • Orhan, G., Orhan, I., Sener, B. 2006. Recent developments in natural and synthetic drug research for Alzheimer’s disease. Letters in Drug Design and Discovery, 3: 268–274.
  • Orhan, I.E., Kartal, M., Gülpinar, A.R., Cos, P., Matheeussen, A., Maes, L., Tasdemir, D., 2013. Assessment of antimicrobial and antiprotozoal activity of the olive oil macerate samples of Hypericum perforatum and their LC-DAD-MS analyses. Food Chemistry, 138: 870–875.
  • Rahiman, S., Tantry, B.A., Kumar, A. 2013. Variation of Antioxidant Activity and Phenolic Content of Some Common Home Remedies with Storage Time. African Journal of Traditional Complementary and Alternative Medicines, 10(1): 124–127.
  • Sagratini, G., Ricciutelli, M., Vittori, S., Öztürk, N., Öztürk, Y., Maggi, Y. 2008. Phytochemical and antioxidant analysis of eight Hypericum taxa from Central Italy Fitoterapia, 79: 210–213.
  • Sánchez-Reus, M.I., Gómez del Rio, M.A., Iglesias, I., Elorza, M., Slowing, K., Benedí, J. 2007. Standardized Hypericum perforatum reduces oxidative stress and increases gene expression of antioxidant enzymes on rotenone-exposed rats. Neuropharmacology, 52 : 606-616.
  • Sedlak, J., Lindsay, R.H. 1968. Estimation of Total protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry, 25(1): 192-205.
  • Silva, B. A., Ferreres, F., Malva, J. O., Dias, A.C.P. 2005. Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry, 90: 157–167.
  • Süntar, I.P., Akkol, E.K., Yılmazer, D., Baykal, T., Kırmızıbekmez, H., Alper, M., Yeşilada, E. 2010. Investigations on the in vivo wound healing potential of Hypericum perforatum L. Journal of Ethnopharmacology, 127: 468-477.
  • Zheng, W., Wang, S. Y. 2001. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry, 49: 5165–5170.
  • Zou, Y., Lu, Y., Wei, D. 2004. Antioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L. in Vitro. Journal of Agricultural and Food Chemistry, 52(16): 5032-5039.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Vesile Düzgüner

Nurcan Erbil This is me

Publication Date January 25, 2020
Submission Date June 14, 2019
Published in Issue Year 2020 Volume: 7 Issue: 1

Cite

APA Düzgüner, V., & Erbil, N. (2020). Ardahan Yöresinde Yetişen Kılıç Otu Bitkisinin (Hypericum perforatum) Antimikrobiyal ve Antioksidan Etkilerinin Araştırılması. Türk Tarım Ve Doğa Bilimleri Dergisi, 7(1), 27-31. https://doi.org/10.30910/turkjans.679896