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Ekinezyada (Echinacea pallida) Farklı Eksplant Tiplerinden İndirekt Sürgün Rejenerasyonu

Year 2020, Volume: 7 Issue: 4, 885 - 895, 20.10.2020

Münüre Tanur Mustafa Yorgancılar

https://doi.org/10.30910/turkjans.784396

Abstract

Araştırmada, Echinacea pallida türünde yaprak, yaprak sapı, kotiledon ve kök eksplantlarından türetilmiş kalluslar aracılığıyla indirekt sürgün rejenerasyonu çalışılmıştır. Dört farklı eksplant tipinden türetilmiş kalluslar, sürgün gelişimini teşvik etmek amacıyla, farklı konsantrasyonlarda (0.1, 0.5, 1.0, 2.0 veya 4.0 mg l-1) ve çeşitte sitokinin (BAP, TDZ, KIN) içeren MS besin ortamlarında kültüre alınmıştır. En yüksek sürgün reenerasyonu, sırasıyla %81 ve %59 ile 0.5 mg l-1 BAP içeren ortamda ve kotiledon eksplantlarından türetilmiş kalluslar ile 1.0 mg l-1 BAP içeren ortamda kök eksplantlarından türetilmiş kalluslardan elde edilmiştir. Eksplant başına en yüksek sürgün sayısı (4.83 adet) 0.5 mg l-1 BAP içeren besin ortamında kök eksplantlarından elde edilen kalluslardan elde edilmiştir. Elde edilen sürgünler, kök gelişimini teşvik etmek ve rejenerasyon sürecini tamamlamak amacıyla farklı konsantrasyonlarda (0.5, 1.0 veya 1.5 mg l-1) ve tiplerde oksin (NAA, IBA, IAA) içeren ve içermeyen MS besin ortamlarında kültüre alınmıştır. En yüksek köklenme oranı (%6) büyüme düzenleyicisi içermeyen ve 0.5 mg l-1 IBA içeren besin ortamından elde edilmiştir. Diğer oksin tipleri ve konsantrasyonlarında köklenme tespit edilmemiştir.

Keywords

echinacea eksplant tipi köklenme organogenesis sürgün rejenerasyonu

Supporting Institution

S.Ü. BİLİMSEL ARAŞTIRMA FONU

Project Number

18101018

Thanks

Bu çalışma, Münüre TANUR ERKOYUNCU’nun doktora tezinin bir parçası olup, Selçuk Üniversitesi Bilimsel Araştırma Fonu tarafından 18101018 no’lu proje kapsamında desteklenmiştir. Desteklerinden dolayı teşekkür ederiz.

References

  • Barnes, J., Anderson, L. A., Gibbons, S. ve Phillipson, J. D. 2005. Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology, 57 (8), 929-954.
  • Barrett, B. 2003. Medicinal properties of Echinacea: a critical review. Phytomedicine, 10 (1), 66-86.
  • Çelik, S. A. ve Kan, Y. 2019. Ekinezya Türlerinde Uçucu Yağ Verim ve Bileşenlerinin Belirlenmesi. Erciyes Tarım ve Hayvan Bilimleri Dergisi, 2 (2), 7-14.
  • Y. Gao, Y., Wu, C.H., Wu., Piao, X.C., Han, L., Gao, R. ve Lian, M.L. 2018. Optimization of culture medium components and culture period for production of adventitious roots of Echinacea pallida (Nutt.) Nutt. Plant Cell, Tissue and Organ Culture (PCTOC), 135:299–307.
  • Harbage, J. F. 2001. Micropropagation of Echinacea angustifolia, E. pallida, and E. purpurea from stem and seed explants. HortScience, 36 (2), 360-364.
  • Jamshidi, M., Barzegar, M. ve Sahari, M.A. 2014. Effect of gamma and microwave irradiation on antioxidant and antimicrobial activities of Cinnamomum zeylanicum and Echinacea purpurea. Int Food Res J., 21:1289–1296.
  • Koroch, A., Kapteyn, J., Juliani, H. ve Simon, J. 2003. In vitro regeneration of Echinacea pallida from leaf explants. In Vitro Cellular & Developmental Biology-Plant, 39 (4), 415-418.
  • Kumar, V., Gururaj, H., Prasad, B. N., Giridhar, P. ve Ravishankar, G. 2005. Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L, Scientia Horticulturae, 106 (2), 237-246.
  • Lakshmanan, P., Danesh, M. ve Taji, A. 2002. Production of four commercially cultivated Echinacea species by different methods of in vitro regeneration. The Journal of Horticultural Science and Biotechnology, 77 (2), 158-163.
  • McKeown, K. A. 1999. A review of the taxonomy of the genus Echinacea. Perspectives on new crops and new uses, 482-489.
  • Mirici, S. 2004. Endemik Geven (Astragalus polemoniacus Bunge) Bitkisinin Yaprak Sapı ve Yaprak Eksplantlarından Yüksek Oranda Adventif Sürgün Rejenerasyonu. Selcuk Journal of Agriculture and Food Sciences, 18 (34), 31-34.
  • Mishima, S., Saito, K., Maruyama, H., Inoue, M., Yamashita, T., Ishida, T. ve Gu, Y. 2004. Antioxidant and immuno-enhancing effects of Echinacea purpurea. Biol Pharm Bull, 27:1004–1009.
  • Murashige, T. ve Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15 (3), 473-497.
  • Raman, P., Patino, L. C. ve Nair, M. G. 2004. Evaluation of metal and microbial contamination in botanical supplements, Journal of Agricultural and Food Chemistry, 52 (26), 7822-7827.
  • Rao, S. R. ve Ravishankar, G. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology advances, 20 (2), 101-153.
  • Shen, X., Kane, M. E. ve Chen, J. 2008. Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars. In Vitro Cell.Dev.Biol.-Plant, 44:282–288.
  • Speroni, E., Govoni, P., Guizzardi, S., Renzulli, C. ve Guerra, M. 2002. Anti-inflammatory and cicatrizing activity of Echinacea pallida Nutt. root extract. Journal of ethnopharmacology, 79 (2), 265-272.
  • Smith, T., Lynch, M., Johnson, J., Kawa, K., Bauman, H. ve Blumenthal, M. 2015. Herbal dietary supplement sales in US increase 6.8% in 2014. HerbalGram, 107, 52-59.
  • Toselli, F., Matthias, A. ve Gillam, E. M. 2009. Echinacea metabolism and drug interactions: the case for standardization of a complementary medicine. Life Sci, 85 (3-4), 97-106.
  • Türker, A. U., Yücesan, B. ve Gürel, E. 2010. Adventitious shoot regeneration from stem internode explants of Verbena officinalis L., a medicinal plant. Turkish Journal of Biology, 34 (3), 297-304.
  • Uranbey, S., Çöçü, S., Sancak, C., Parmaksız, İ., Khawar, K., Mirici, S. ve Özcan, S. 2003. Efficient adventitious shoot regeneration in cicer milkvetch. Biotechnology & Biotechnological Equipment, 17 (1), 33-37.
  • Wang, H.M., Jeng, S.T. ve To, K. Y. 2017. In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida. Plant Cell Tiss Organ Cult., 130:117–130.
  • Wichtl, M. 2004. Herbal drugs and phytopharmaceuticals: a handbook for practice on a scientific basis. Ed. 3, Medpharm GmbH Scientific Publishers.
  • Yucesan, B., Turker, A. U. ve Gurel, E. 2007. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant cell, tissue and organ culture, 91 (3), 243-250.

Year 2020, Volume: 7 Issue: 4, 885 - 895, 20.10.2020

Münüre Tanur Mustafa Yorgancılar

https://doi.org/10.30910/turkjans.784396

Abstract

Project Number

18101018

References

  • Barnes, J., Anderson, L. A., Gibbons, S. ve Phillipson, J. D. 2005. Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology, 57 (8), 929-954.
  • Barrett, B. 2003. Medicinal properties of Echinacea: a critical review. Phytomedicine, 10 (1), 66-86.
  • Çelik, S. A. ve Kan, Y. 2019. Ekinezya Türlerinde Uçucu Yağ Verim ve Bileşenlerinin Belirlenmesi. Erciyes Tarım ve Hayvan Bilimleri Dergisi, 2 (2), 7-14.
  • Y. Gao, Y., Wu, C.H., Wu., Piao, X.C., Han, L., Gao, R. ve Lian, M.L. 2018. Optimization of culture medium components and culture period for production of adventitious roots of Echinacea pallida (Nutt.) Nutt. Plant Cell, Tissue and Organ Culture (PCTOC), 135:299–307.
  • Harbage, J. F. 2001. Micropropagation of Echinacea angustifolia, E. pallida, and E. purpurea from stem and seed explants. HortScience, 36 (2), 360-364.
  • Jamshidi, M., Barzegar, M. ve Sahari, M.A. 2014. Effect of gamma and microwave irradiation on antioxidant and antimicrobial activities of Cinnamomum zeylanicum and Echinacea purpurea. Int Food Res J., 21:1289–1296.
  • Koroch, A., Kapteyn, J., Juliani, H. ve Simon, J. 2003. In vitro regeneration of Echinacea pallida from leaf explants. In Vitro Cellular & Developmental Biology-Plant, 39 (4), 415-418.
  • Kumar, V., Gururaj, H., Prasad, B. N., Giridhar, P. ve Ravishankar, G. 2005. Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L, Scientia Horticulturae, 106 (2), 237-246.
  • Lakshmanan, P., Danesh, M. ve Taji, A. 2002. Production of four commercially cultivated Echinacea species by different methods of in vitro regeneration. The Journal of Horticultural Science and Biotechnology, 77 (2), 158-163.
  • McKeown, K. A. 1999. A review of the taxonomy of the genus Echinacea. Perspectives on new crops and new uses, 482-489.
  • Mirici, S. 2004. Endemik Geven (Astragalus polemoniacus Bunge) Bitkisinin Yaprak Sapı ve Yaprak Eksplantlarından Yüksek Oranda Adventif Sürgün Rejenerasyonu. Selcuk Journal of Agriculture and Food Sciences, 18 (34), 31-34.
  • Mishima, S., Saito, K., Maruyama, H., Inoue, M., Yamashita, T., Ishida, T. ve Gu, Y. 2004. Antioxidant and immuno-enhancing effects of Echinacea purpurea. Biol Pharm Bull, 27:1004–1009.
  • Murashige, T. ve Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15 (3), 473-497.
  • Raman, P., Patino, L. C. ve Nair, M. G. 2004. Evaluation of metal and microbial contamination in botanical supplements, Journal of Agricultural and Food Chemistry, 52 (26), 7822-7827.
  • Rao, S. R. ve Ravishankar, G. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology advances, 20 (2), 101-153.
  • Shen, X., Kane, M. E. ve Chen, J. 2008. Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars. In Vitro Cell.Dev.Biol.-Plant, 44:282–288.
  • Speroni, E., Govoni, P., Guizzardi, S., Renzulli, C. ve Guerra, M. 2002. Anti-inflammatory and cicatrizing activity of Echinacea pallida Nutt. root extract. Journal of ethnopharmacology, 79 (2), 265-272.
  • Smith, T., Lynch, M., Johnson, J., Kawa, K., Bauman, H. ve Blumenthal, M. 2015. Herbal dietary supplement sales in US increase 6.8% in 2014. HerbalGram, 107, 52-59.
  • Toselli, F., Matthias, A. ve Gillam, E. M. 2009. Echinacea metabolism and drug interactions: the case for standardization of a complementary medicine. Life Sci, 85 (3-4), 97-106.
  • Türker, A. U., Yücesan, B. ve Gürel, E. 2010. Adventitious shoot regeneration from stem internode explants of Verbena officinalis L., a medicinal plant. Turkish Journal of Biology, 34 (3), 297-304.
  • Uranbey, S., Çöçü, S., Sancak, C., Parmaksız, İ., Khawar, K., Mirici, S. ve Özcan, S. 2003. Efficient adventitious shoot regeneration in cicer milkvetch. Biotechnology & Biotechnological Equipment, 17 (1), 33-37.
  • Wang, H.M., Jeng, S.T. ve To, K. Y. 2017. In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida. Plant Cell Tiss Organ Cult., 130:117–130.
  • Wichtl, M. 2004. Herbal drugs and phytopharmaceuticals: a handbook for practice on a scientific basis. Ed. 3, Medpharm GmbH Scientific Publishers.
  • Yucesan, B., Turker, A. U. ve Gurel, E. 2007. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant cell, tissue and organ culture, 91 (3), 243-250.
There are 24 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Münüre Tanur 0000-0001-5004-4771

Mustafa Yorgancılar 0000-0003-4938-8547

Project Number 18101018
Publication Date October 20, 2020
Submission Date August 23, 2020
Published in Issue Year 2020 Volume: 7 Issue: 4

Cite

APA Tanur, M., & Yorgancılar, M. (2020). Ekinezyada (Echinacea pallida) Farklı Eksplant Tiplerinden İndirekt Sürgün Rejenerasyonu. Türk Tarım Ve Doğa Bilimleri Dergisi, 7(4), 885-895. https://doi.org/10.30910/turkjans.784396
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