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Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars

Year 2022, Volume: 9 Issue: 2, 469 - 476, 23.04.2022
https://doi.org/10.30910/turkjans.1063279

Abstract

Leguminous Broad bean (Vicia faba L.) is an excellent source of minerals, vitamins and proteins that makes it higly valuable food for human and feed for animal consumption. The plant has an important characteristic to fix atmospheric Nitrogen and play an important role to establish a natural balance of N in the atmosphere. It is a highly self-pollinated plant and has problem of low variation with limited genetic pool. Moreover, the previous studies identify problem of recalcitrance in broad bean. Therefore, there is need to establish a repeatable micropropagation protocol that could ensure an increase in genetic variability to overwhelm problems in breeding. This system must also be able for efficient gene delivery and could be integrated with the conventional breeding programs through direct organogenesis. The study aimed to develop a tissue culture protocol on two important Turkish broad bean cultivars Filiz99 and Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mg l-1 TDZ using embryonic axis as explant. Maximum number of 5.33 and 3 shoots per explant were noted on cv. Filiz99 and Eresen87 on MS medium containing 0.15 mg l-1 TDZ. The developing shoots were rooted on MS medium containing 1 mg l-1 IAA after three weeks of culture. The rooted plants were transferred to pots containing peat under maintained under controlled greenhouse conditions for acclimatization The acclimatized plants bloomed and set seeds. Present results underscore importance of seed hydropriming before taking of explants to achieve high micropropagation on faba beans to overcome recalcitrance.

References

  • Abdelwahd, R., Hakam, N., Labhilili, M., Udupa, M. 2008. Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in In vitro plantlet regeneration of faba bean. African Journal of Biotechnology. 2008; 7: 997-1002.
  • Abdelwahd, R., Udupa, M.S., Gaboun, F., Diria, G., Mentag, R., Ibriz, M., Iraqi, D. 2014. Agrobacterıum-mediated transformation of cotyledonary node of Vicia faba L. Romanıan Agrıcultural Research, Nardi Fundulea, Romania. 31: 2067-5720.
  • Almaghrabi, O.A. 2014. Effect of growth hormone 2,4-D on some callus traits of different faba bean (Vicia faba L.) cultivars. Life Sciences. 11: 98 – 102.
  • Anwar, F. 2007. Genetic transformation of chickpea with bacterial codA gene enhancing drought tolerance. Ph.D. Thesis, University of Delhi, Delhi, India. Anwar, F., Sharmila, P., Pardha, S.P. 2010. No more recalcitrant: Chickpea regeneration and genetic transformation. African Journal of Biotechnology. 9: 782-797.
  • Bahgat, S., Shabban, A.O., El-Shihy, O., Lightfoot, A.D., El-Shemyi, A.H. 2009. Establishment of the regeneration system for Vicia faba L. Current Issues in Molecular Biology. 11: (Suppl. 1). 47–54.
  • Bond, D.A. 1987. Recent developments in breeding field beans (Vicia faba L.). Plant Breeding. 99: 1–26.
  • Böttinger, P., Steinmetz, A., Schieder, O., Pickardt, T. 2001. Agrobacterium-mediated transformation of Vicia faba. Molecular Breeding. 8: 243-254.
  • Christou, P. 1994. The Biotechnology of Crop Legumes. Euphytica. 74: 165-185.
  • Edyta, A., Liona, C., Izaela, I. 2012. Indirect organogenesis of faba bean (Vicia faba L. Minor). Acta Biologica Cracoviensia Series Botanica. 54: 102–108.
  • Fahn, A. 1977. Plant Anatomy. Pergamon Press. Oxford. 2. 13.
  • Ford, Y., Bonham. Y.E.C., Cameron. P.S., Blake, H.L. 2001. Adventitious rooting: examining the role of auxin in an easy and a difficult-to-root plant. Plant Growth Regul. 36:1-11.
  • Fukaki, H., Tasaka, M. 2009. Hormon interactions during lateral root formation. Plant Molecular Biology. 69: 437-49.
  • Hanafy, M., Thomas. P., Heiko, K,. Jacobsen, H.J. 2005. Agrobacterium-mediated Transformation of Faba bean (Vicia faba L.) using embriyo axes. Euphytica. 142, 227- 236.
  • Klenotıčova, H., Smykalova, I., Švabova, L., Griga, M. 2013. Resolving browning during the establishment of explant cultures in Vicia faba L. for Genetic Transformation. Acta Universitatis Agriculturae Facultas Silviculturae. Mendelianae Brunensis. 5: 1279–1288.
  • Metry, E.A., Ismail, R.M., Hussien, G.M., Nasr El-Din, T.M., El-Itriby, H.A. 2007. Regeneration and microprojectile - mediated transformation in Vicia faba L. Arab journal of biotechnology.10: 23-36.
  • Miean, K.H., Mohamed, S. 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. Journal of Agricultural and Food Chemistry. 49: 3106-12.
  • Nofouzi, F., Çağri OĞUZ, M., Delpasand Khabbazi, S., Ergül, A. 2019. Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L. Turkish Journal of Agriculture and Forestry. 43: 96-104.
  • Roba, M.I., Heba, E.M.E., Gihan, M.H.H., Emad, A.M. 2011. In vitro root induction of faba bean (Vicia faba L.). GM Crops & Food. 2: 176-181.
  • Selva, E., Stouffs, B., Briquet, M. 1989. In vitro propagation of Vicia faba L. by micro-cutting and multiple shoot induction. Plant Cell, Tissue and Organ Culture. 18: 167-179.
  • Shimaa, B., Omer, A., Osama, E., David, A., Hany, E. 2008. Establishment of the regeneration system for Vicia faba L. Current Issues in Molecular Biology. 11: 47–54.
  • Srinath, R., Patil, R., Kaviraj, C. 2005. Callus induction and organogenesis from various explants in Vigna radiate (L.) Wilczek. Indian Journal of Biotechnology. 4: 556-560.
  • Taha, R.M., Francis, D. 1990. The relationship between polyploidy and organogenetic potential in embryo- and root-derived tissue culture of Vicia faba L. Plant Cell, Tissue and Organ Culture. 22: 229-236.
  • Takahoshni, W., Takomizo, T. 2012. Molecular breeding of grasses by transgenic approaches for biofuels production. In Transgenic Plants-Advances and Limitation, Y.O., Cafici ed. (Pijeka, Croutia: In Tech), pp. 91-116.

Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars

Year 2022, Volume: 9 Issue: 2, 469 - 476, 23.04.2022
https://doi.org/10.30910/turkjans.1063279

Abstract

Leguminous Broad bean (Vicia faba L.) is an excellent source of minerals, vitamins and proteins that makes it higly valuable food for human and feed for animal consumption. The plant has an important characteristic to fix atmospheric Nitrogen and play an important role to establish a natural balance of N in the atmosphere. It is a highly self-pollinated plant and has problem of low variation with limited genetic pool. Moreover, the previous studies identify problem of recalcitrance in broad bean. Therefore, there is need to establish a repeatable micropropagation protocol that could ensure an increase in genetic variability to overwhelm problems in breeding. This system must also be able for efficient gene delivery and could be integrated with the conventional breeding programs through direct organogenesis. The study aimed to develop a tissue culture protocol on two important Turkish broad bean cultivars Filiz99 and Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mg l-1 TDZ using embryonic axis as explant. Maximum number of 5.33 and 3 shoots per explant were noted on cv. Filiz99 and Eresen87 on MS medium containing 0.15 mg l-1 TDZ. The developing shoots were rooted on MS medium containing 1 mg l-1 IAA after three weeks of culture. The rooted plants were transferred to pots containing peat under maintained under controlled greenhouse conditions for acclimatization The acclimatized plants bloomed and set seeds. Present results underscore importance of seed hydropriming before taking of explants to achieve high micropropagation on faba beans to overcome recalcitrance.

References

  • Abdelwahd, R., Hakam, N., Labhilili, M., Udupa, M. 2008. Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in In vitro plantlet regeneration of faba bean. African Journal of Biotechnology. 2008; 7: 997-1002.
  • Abdelwahd, R., Udupa, M.S., Gaboun, F., Diria, G., Mentag, R., Ibriz, M., Iraqi, D. 2014. Agrobacterıum-mediated transformation of cotyledonary node of Vicia faba L. Romanıan Agrıcultural Research, Nardi Fundulea, Romania. 31: 2067-5720.
  • Almaghrabi, O.A. 2014. Effect of growth hormone 2,4-D on some callus traits of different faba bean (Vicia faba L.) cultivars. Life Sciences. 11: 98 – 102.
  • Anwar, F. 2007. Genetic transformation of chickpea with bacterial codA gene enhancing drought tolerance. Ph.D. Thesis, University of Delhi, Delhi, India. Anwar, F., Sharmila, P., Pardha, S.P. 2010. No more recalcitrant: Chickpea regeneration and genetic transformation. African Journal of Biotechnology. 9: 782-797.
  • Bahgat, S., Shabban, A.O., El-Shihy, O., Lightfoot, A.D., El-Shemyi, A.H. 2009. Establishment of the regeneration system for Vicia faba L. Current Issues in Molecular Biology. 11: (Suppl. 1). 47–54.
  • Bond, D.A. 1987. Recent developments in breeding field beans (Vicia faba L.). Plant Breeding. 99: 1–26.
  • Böttinger, P., Steinmetz, A., Schieder, O., Pickardt, T. 2001. Agrobacterium-mediated transformation of Vicia faba. Molecular Breeding. 8: 243-254.
  • Christou, P. 1994. The Biotechnology of Crop Legumes. Euphytica. 74: 165-185.
  • Edyta, A., Liona, C., Izaela, I. 2012. Indirect organogenesis of faba bean (Vicia faba L. Minor). Acta Biologica Cracoviensia Series Botanica. 54: 102–108.
  • Fahn, A. 1977. Plant Anatomy. Pergamon Press. Oxford. 2. 13.
  • Ford, Y., Bonham. Y.E.C., Cameron. P.S., Blake, H.L. 2001. Adventitious rooting: examining the role of auxin in an easy and a difficult-to-root plant. Plant Growth Regul. 36:1-11.
  • Fukaki, H., Tasaka, M. 2009. Hormon interactions during lateral root formation. Plant Molecular Biology. 69: 437-49.
  • Hanafy, M., Thomas. P., Heiko, K,. Jacobsen, H.J. 2005. Agrobacterium-mediated Transformation of Faba bean (Vicia faba L.) using embriyo axes. Euphytica. 142, 227- 236.
  • Klenotıčova, H., Smykalova, I., Švabova, L., Griga, M. 2013. Resolving browning during the establishment of explant cultures in Vicia faba L. for Genetic Transformation. Acta Universitatis Agriculturae Facultas Silviculturae. Mendelianae Brunensis. 5: 1279–1288.
  • Metry, E.A., Ismail, R.M., Hussien, G.M., Nasr El-Din, T.M., El-Itriby, H.A. 2007. Regeneration and microprojectile - mediated transformation in Vicia faba L. Arab journal of biotechnology.10: 23-36.
  • Miean, K.H., Mohamed, S. 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. Journal of Agricultural and Food Chemistry. 49: 3106-12.
  • Nofouzi, F., Çağri OĞUZ, M., Delpasand Khabbazi, S., Ergül, A. 2019. Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L. Turkish Journal of Agriculture and Forestry. 43: 96-104.
  • Roba, M.I., Heba, E.M.E., Gihan, M.H.H., Emad, A.M. 2011. In vitro root induction of faba bean (Vicia faba L.). GM Crops & Food. 2: 176-181.
  • Selva, E., Stouffs, B., Briquet, M. 1989. In vitro propagation of Vicia faba L. by micro-cutting and multiple shoot induction. Plant Cell, Tissue and Organ Culture. 18: 167-179.
  • Shimaa, B., Omer, A., Osama, E., David, A., Hany, E. 2008. Establishment of the regeneration system for Vicia faba L. Current Issues in Molecular Biology. 11: 47–54.
  • Srinath, R., Patil, R., Kaviraj, C. 2005. Callus induction and organogenesis from various explants in Vigna radiate (L.) Wilczek. Indian Journal of Biotechnology. 4: 556-560.
  • Taha, R.M., Francis, D. 1990. The relationship between polyploidy and organogenetic potential in embryo- and root-derived tissue culture of Vicia faba L. Plant Cell, Tissue and Organ Culture. 22: 229-236.
  • Takahoshni, W., Takomizo, T. 2012. Molecular breeding of grasses by transgenic approaches for biofuels production. In Transgenic Plants-Advances and Limitation, Y.O., Cafici ed. (Pijeka, Croutia: In Tech), pp. 91-116.
There are 23 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Articles
Authors

Ferzat Turan 0000-0001-5960-6478

Khalıd Mahmood Khawar 0000-0001-5110-6014

Publication Date April 23, 2022
Submission Date January 26, 2022
Published in Issue Year 2022 Volume: 9 Issue: 2

Cite

APA Turan, F., & Khawar, K. M. (2022). Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars. Türk Tarım Ve Doğa Bilimleri Dergisi, 9(2), 469-476. https://doi.org/10.30910/turkjans.1063279